A Chromosome Assay Method for the Detection of
... evidence for the diploid nature of the RD6 material. A progeny sample of 222 haploid segregants (RD6-1 to RD6-222) was collected. The segregants were purified, transferred to 4 x 4 master plates and replicated on to a range of differential media to analyse the segregation of standard markers (Table ...
... evidence for the diploid nature of the RD6 material. A progeny sample of 222 haploid segregants (RD6-1 to RD6-222) was collected. The segregants were purified, transferred to 4 x 4 master plates and replicated on to a range of differential media to analyse the segregation of standard markers (Table ...
A Novel Multigene Family May Encode Odorant Receptors: A
... How are the diversity and specificity of olfactory perception accomplished? The detection of chemically distinct odorants presumably results from the association of odorous ligands with specific receptors on olfactory neurons, which reside in a specialized epithelium in the nose. Since these recepto ...
... How are the diversity and specificity of olfactory perception accomplished? The detection of chemically distinct odorants presumably results from the association of odorous ligands with specific receptors on olfactory neurons, which reside in a specialized epithelium in the nose. Since these recepto ...
Sex-specific Trans-regulatory Variation on the Drosophila melanogaster X Chromosome
... genes, n = 121), and another set of genes that shows a high degree of sexually concordant genetic variation (SCV genes, n = 151) (S1 Fig.; S1 Table). SDV and SCV genes were evenly distributed, with respect to each other, over the X chromosome and the autosomes (twoproportion z-test: χ2 = 0, d.f. = 1 ...
... genes, n = 121), and another set of genes that shows a high degree of sexually concordant genetic variation (SCV genes, n = 151) (S1 Fig.; S1 Table). SDV and SCV genes were evenly distributed, with respect to each other, over the X chromosome and the autosomes (twoproportion z-test: χ2 = 0, d.f. = 1 ...
Protein A gene expression is regulated by DNA supercoiling which
... As the expression of the protein A gene is dramatically repressed by high salt concentrations (1 M NaCl) and as high osmolarity alters the degree of plasmid DNA supercoiling, we looked at the effect of changing the degree of DNA supercoiling on protein A gene expression. We first determined the conc ...
... As the expression of the protein A gene is dramatically repressed by high salt concentrations (1 M NaCl) and as high osmolarity alters the degree of plasmid DNA supercoiling, we looked at the effect of changing the degree of DNA supercoiling on protein A gene expression. We first determined the conc ...
Phenotypic Stability of trp Operon Recombinant
... Plasmid preparatiorr. Escherichia coli carrying RSF2124-trp was grown ovei night in PBB and diluted 10fold in 100 ml M-9 medium (Clowes & Hayes, 1968) supplemented with Casamino acids (20 g 1-lj and thiamin (1Opg ml-l) or thymine (1Opg ml-l), when required. The culture was grown for 2 h a t 37 "Cwit ...
... Plasmid preparatiorr. Escherichia coli carrying RSF2124-trp was grown ovei night in PBB and diluted 10fold in 100 ml M-9 medium (Clowes & Hayes, 1968) supplemented with Casamino acids (20 g 1-lj and thiamin (1Opg ml-l) or thymine (1Opg ml-l), when required. The culture was grown for 2 h a t 37 "Cwit ...
Ribosome profiling reveals post-transcriptional buffering of divergent
... (A) Ribosome profiling was performed on log-phase cultures of S. cerevisiae, S. paradoxus, and their F1 hybrid. Ribosome protected fragments (RPFs) were purified and cloned into Illumina high-throughput sequencing libraries (left). Poly-adenylated mRNA sequencing libraries were prepared in parallel ...
... (A) Ribosome profiling was performed on log-phase cultures of S. cerevisiae, S. paradoxus, and their F1 hybrid. Ribosome protected fragments (RPFs) were purified and cloned into Illumina high-throughput sequencing libraries (left). Poly-adenylated mRNA sequencing libraries were prepared in parallel ...
MEIOSIS I
... Inheritance of Genes Genes are the units of heredity and are made up of segments of DNA Genes are passed to the next generation via reproductive cells called gametes (sperm and eggs) ...
... Inheritance of Genes Genes are the units of heredity and are made up of segments of DNA Genes are passed to the next generation via reproductive cells called gametes (sperm and eggs) ...
Plant centromeres: structure and control Eric J Richards and R Kelly
... controlling and/or mediating sister chromatid cohesion in metaphase (of mitosis and meiosis II) [19,20]. A number of highly reiterated repeats that reside in centromere regions in plant species have been described [21–24,25•,26•,27,28] (see Table 1 for a partial list). Because most of these DNA repe ...
... controlling and/or mediating sister chromatid cohesion in metaphase (of mitosis and meiosis II) [19,20]. A number of highly reiterated repeats that reside in centromere regions in plant species have been described [21–24,25•,26•,27,28] (see Table 1 for a partial list). Because most of these DNA repe ...
Renal Cell Carcinoma: Recent Advances in Genetics and Imaging
... with types 1 and 2 B VHL disease are at high risk of developing clear cell RCCs. Clear cell RCCs occur in 75% of VHL patients by the sixth decade and are a leading cause of morbidity and mortality.13 VHL gene encodes for pVHL (VHL protein), a key component of cellular oxygen homeostatic mechanism. A ...
... with types 1 and 2 B VHL disease are at high risk of developing clear cell RCCs. Clear cell RCCs occur in 75% of VHL patients by the sixth decade and are a leading cause of morbidity and mortality.13 VHL gene encodes for pVHL (VHL protein), a key component of cellular oxygen homeostatic mechanism. A ...
Homologous Recombination Between Episomal Plasmids and Chromosomes in Yeast.
... otherwise, there seems to be no difference between the cell types. Finally, we examined the effect of sunlamp radiation upon plasmid-chromosome recombination. These results are also shown in Table 3. As reported 1974), sunlamp radiation stimulates previously (LAWRENCE and CHRISTENSEN mitotic recombi ...
... otherwise, there seems to be no difference between the cell types. Finally, we examined the effect of sunlamp radiation upon plasmid-chromosome recombination. These results are also shown in Table 3. As reported 1974), sunlamp radiation stimulates previously (LAWRENCE and CHRISTENSEN mitotic recombi ...
- Lab Fabiana Perocchi
... ADE is a consequence of cis-regulatory variation, which by definition, acts on the allele of the same chromosome (Knight, & 2009 EMBO and Macmillan Publishers Limited ...
... ADE is a consequence of cis-regulatory variation, which by definition, acts on the allele of the same chromosome (Knight, & 2009 EMBO and Macmillan Publishers Limited ...
PKB - Friedrich Miescher Institute for Biomedical Research
... Phosphate and Tensin Homolog Deleted on Chromosome 10 Protein (PTEN) • Protein that controls cell growth and division via regulation of PKB activation • Tumour suppressor protein • 2nd most frequently mutated tumour suppressor in cancer after p53 (Lee et al. 1999) ...
... Phosphate and Tensin Homolog Deleted on Chromosome 10 Protein (PTEN) • Protein that controls cell growth and division via regulation of PKB activation • Tumour suppressor protein • 2nd most frequently mutated tumour suppressor in cancer after p53 (Lee et al. 1999) ...
Disintegrin, hemorrhagic, and proteolytic activities of Mohave
... They inhibit cell – cell, cell – matrix interactions and signal transduction (McLane et al., 2004). Due to their binding abilities, disintegrins have a great potential in biomedical applications such as inhibiting angiogenesis and tumor progression in vivo (Markland and Zhou, 2000). However, heretof ...
... They inhibit cell – cell, cell – matrix interactions and signal transduction (McLane et al., 2004). Due to their binding abilities, disintegrins have a great potential in biomedical applications such as inhibiting angiogenesis and tumor progression in vivo (Markland and Zhou, 2000). However, heretof ...
Williams syndrome deficits in visual spatial Chromosome 7q11.23
... All three subjects in our study were ascertained initially by cardiovascular anomalies. The features of Williams syndrome including the facial features are quite distinct to Japanese physicians. However, the typical physical features of individuals with WS were not observed in our three subjects. Th ...
... All three subjects in our study were ascertained initially by cardiovascular anomalies. The features of Williams syndrome including the facial features are quite distinct to Japanese physicians. However, the typical physical features of individuals with WS were not observed in our three subjects. Th ...
CONSERVATION AND DIVERGENCE IN MOLECULAR
... (165). All four of the maternal coordinate systems are set up during oogenesis, and recognition of and elaboration upon axial maternal cues are among the earliest events after egg activation and fertilization. The anterior system involves cytoplasmic diffusion of morphogens within the syncytial D. m ...
... (165). All four of the maternal coordinate systems are set up during oogenesis, and recognition of and elaboration upon axial maternal cues are among the earliest events after egg activation and fertilization. The anterior system involves cytoplasmic diffusion of morphogens within the syncytial D. m ...
antibody anarchy: a call to order
... is mass spectrometry and the other is a beadbased array with thousands of antibodies. The mass spectrometry data serve as a reference for the results obtained with antibodies. Turning the idea into a refined assay will take considerable work, Lund-Johansen admits. “It is extremely ambitious. It is t ...
... is mass spectrometry and the other is a beadbased array with thousands of antibodies. The mass spectrometry data serve as a reference for the results obtained with antibodies. Turning the idea into a refined assay will take considerable work, Lund-Johansen admits. “It is extremely ambitious. It is t ...
Assembly factors as a new class of disease
... and NDUFS8). Despite the lack of mechanistic data, the stages in which the other assembly factors operate can be derived from the accumulation of assembly intermediates upon their mutation or knockdown, and by examining their protein–protein interactions by affinity purification. The proposed involv ...
... and NDUFS8). Despite the lack of mechanistic data, the stages in which the other assembly factors operate can be derived from the accumulation of assembly intermediates upon their mutation or knockdown, and by examining their protein–protein interactions by affinity purification. The proposed involv ...
Chromosomal breakpoint positions suggest a direct role for radiation
... properties, but was not sucient for transformation (Santoro et al., 1993). However, malignant phenotype was obtained by cotransfection with H-RAS or K-RAS oncogenes. An alternative to the direct pathway is that radiation does not act directly to produce the RET/PTC3 fusion, but instead may cause pe ...
... properties, but was not sucient for transformation (Santoro et al., 1993). However, malignant phenotype was obtained by cotransfection with H-RAS or K-RAS oncogenes. An alternative to the direct pathway is that radiation does not act directly to produce the RET/PTC3 fusion, but instead may cause pe ...
Document
... permeability and reduced plasma lipopolysaccharide (LPS) suggesting that the prebiotic diet reduces uptake of bacterial products from the gut lumen. As gut permeability can be regulated at the level of the tight junction between epithelial cells, the investigators examined the expression of the tigh ...
... permeability and reduced plasma lipopolysaccharide (LPS) suggesting that the prebiotic diet reduces uptake of bacterial products from the gut lumen. As gut permeability can be regulated at the level of the tight junction between epithelial cells, the investigators examined the expression of the tigh ...
- University of Bath Opus
... and Ward, 2005) and Drosophila(Gnad and Parsch, 2006) genomes demonstrates an increased number of male-biased genes through duplication. DNA sequences with no coding function could also provide a source for sex-biased expression gene generation. Levine et al. (2006) performed a whole-genome study of ...
... and Ward, 2005) and Drosophila(Gnad and Parsch, 2006) genomes demonstrates an increased number of male-biased genes through duplication. DNA sequences with no coding function could also provide a source for sex-biased expression gene generation. Levine et al. (2006) performed a whole-genome study of ...
Relation of the Wnt/β-catenin signaling pathway
... pathway continues under control, biologic processes like proliferation, differentiation, and regulation of target genes’ transcription occur normally, but these target genes’ aberrant activation causes cancer and other serious diseases. In addition, by the activation of transcription of genes that e ...
... pathway continues under control, biologic processes like proliferation, differentiation, and regulation of target genes’ transcription occur normally, but these target genes’ aberrant activation causes cancer and other serious diseases. In addition, by the activation of transcription of genes that e ...
THE Rh BLOOD GROUP SYSTEM
... performed on the Rh-negative mother before the delivery of the infant if the facility uses microscopic reading of the weak D test, or a rosetting test with anti-D, to detect large D fetal–maternal bleed after delivery. Testing for weak D in an apparent D-negative patient needing large-volume or lon ...
... performed on the Rh-negative mother before the delivery of the infant if the facility uses microscopic reading of the weak D test, or a rosetting test with anti-D, to detect large D fetal–maternal bleed after delivery. Testing for weak D in an apparent D-negative patient needing large-volume or lon ...
Extrapolation to the whole human genome
... pseudogenes based on whether there is a continuous span of homology that is >70% of the length of the closest matching human protein (i.e. with introns removed), or whether there is evidence of polyadenylation. We have applied our approach to chromosomes 21 and 22, the first parts of the human genom ...
... pseudogenes based on whether there is a continuous span of homology that is >70% of the length of the closest matching human protein (i.e. with introns removed), or whether there is evidence of polyadenylation. We have applied our approach to chromosomes 21 and 22, the first parts of the human genom ...
Supplemental Material
... also posted at the AAA site, were also used in this analysis. The FlyBase inferred cytological map locations were assigned to all of the orthologs called in the four species. These associations were then ordered and sorted according to their scaffold assignments and molecular coordinates for each sp ...
... also posted at the AAA site, were also used in this analysis. The FlyBase inferred cytological map locations were assigned to all of the orthologs called in the four species. These associations were then ordered and sorted according to their scaffold assignments and molecular coordinates for each sp ...
Three epigenetic information channels and their different roles in
... classified together mechanistically – for example as being based on DNA methylation – will have quite different evolutionary consequences depending upon the way in which they are deployed. Amongst those transgenerational epigenetic effects that are adaptive and have been selected, we distinguish bet ...
... classified together mechanistically – for example as being based on DNA methylation – will have quite different evolutionary consequences depending upon the way in which they are deployed. Amongst those transgenerational epigenetic effects that are adaptive and have been selected, we distinguish bet ...