December 8, 2006 - Brandeis University
... suppression of the Ptpmeg rough-eye phenotype. Vulcan was expected to regulate protein function level rather than gene level expression since the Ptpmeg constructs over-expressed Ptpmeg with a recombinant promoter, introduced with P elements. After analyzing Vulcan’s conserved domain, as shown in Ta ...
... suppression of the Ptpmeg rough-eye phenotype. Vulcan was expected to regulate protein function level rather than gene level expression since the Ptpmeg constructs over-expressed Ptpmeg with a recombinant promoter, introduced with P elements. After analyzing Vulcan’s conserved domain, as shown in Ta ...
The role of Dby mRNA in early development of male mouse zygotes
... Ejaculated mammalian spermatozoa contain a complex yet specific population of mRNA. However, the possible roles that mRNA has in early zygotic and embryonic development remain unclear. We found that Dby mRNA is selectively retained in capacitated mouse spermatozoa, and is transferred into the oocyte ...
... Ejaculated mammalian spermatozoa contain a complex yet specific population of mRNA. However, the possible roles that mRNA has in early zygotic and embryonic development remain unclear. We found that Dby mRNA is selectively retained in capacitated mouse spermatozoa, and is transferred into the oocyte ...
PowerPoint Slides - Evo-Ed
... same or similar license to this one. With the understanding that: Waiver — Any of the above conditions can be waived if you get permission from Jim Smith, Merle Heidemann or Peter White at Michigan State University, [email protected]. Public Domain — Where the work or any of its elements is in the publi ...
... same or similar license to this one. With the understanding that: Waiver — Any of the above conditions can be waived if you get permission from Jim Smith, Merle Heidemann or Peter White at Michigan State University, [email protected]. Public Domain — Where the work or any of its elements is in the publi ...
meiosis I - CARNES AP BIO
... Sets of Chromosomes in Human Cells • Human somatic cells (any cell other than a gamete) have 23 pairs of chromosomes. • A karyotype is an ordered display of the pairs of chromosomes from a cell . • The two chromosomes in each pair are called homologous chromosomes, or homologs. ...
... Sets of Chromosomes in Human Cells • Human somatic cells (any cell other than a gamete) have 23 pairs of chromosomes. • A karyotype is an ordered display of the pairs of chromosomes from a cell . • The two chromosomes in each pair are called homologous chromosomes, or homologs. ...
The murine homologue of HIRA, a DiGeorge
... gene HIRA because the most significant peptide homology found at the time was with Hir1p and Hir2p, two histone gene repressor proteins from the yeast Saccharomyces cerevisiae (32). The predicted HIRA protein is characterized by the presence of seven N-terminal WD40 repeats, two bipartite nuclear lo ...
... gene HIRA because the most significant peptide homology found at the time was with Hir1p and Hir2p, two histone gene repressor proteins from the yeast Saccharomyces cerevisiae (32). The predicted HIRA protein is characterized by the presence of seven N-terminal WD40 repeats, two bipartite nuclear lo ...
Green Fluorescent Protein-Glucocorticoid Receptor Knockin Mice
... manipulation of GR levels can have an impact on this phenomenon, one testable hypothesis is that relative levels of endogenous GR set the threshold for sensitivity to steroid-induced apoptosis. Studies using receptor binding techniques and intracellular immunofluorescent staining to address this hyp ...
... manipulation of GR levels can have an impact on this phenomenon, one testable hypothesis is that relative levels of endogenous GR set the threshold for sensitivity to steroid-induced apoptosis. Studies using receptor binding techniques and intracellular immunofluorescent staining to address this hyp ...
shows altered stratification and cell polarity
... kinase (JNK) (26–28). The loss of Vangl2 as well as other components of PCP results in Pr–Di limb defects (26,29–31). At the ...
... kinase (JNK) (26–28). The loss of Vangl2 as well as other components of PCP results in Pr–Di limb defects (26,29–31). At the ...
Seed-Specific Gene Activation Mediated by the Cre//ox Site
... Inactive marker GUS genes with a lox-bounded blocking fragment (Iox’) between the promoter and coding; region were constructed as follows (Fig. 1B). Before adding ihe Iox2 fragment to each promoter, the NcoI site at the 3’ end of each promoter was deleted so that there would be no translationinitiat ...
... Inactive marker GUS genes with a lox-bounded blocking fragment (Iox’) between the promoter and coding; region were constructed as follows (Fig. 1B). Before adding ihe Iox2 fragment to each promoter, the NcoI site at the 3’ end of each promoter was deleted so that there would be no translationinitiat ...
Spatially restricted domains of homeo-gene
... a segmentally restricted manner and that their expression presages morphological differentiation of segmental structures (e.g. Levine et a|.1983; Akam & Martinez-Arias, 1985; Chadwick & McGinnis, 1987). The discovery of homeo-genes in a wide variety of metazoz, including vertebrates, led to the sugg ...
... a segmentally restricted manner and that their expression presages morphological differentiation of segmental structures (e.g. Levine et a|.1983; Akam & Martinez-Arias, 1985; Chadwick & McGinnis, 1987). The discovery of homeo-genes in a wide variety of metazoz, including vertebrates, led to the sugg ...
Genetic Basis for Osmosensitivity and Genetic Instability of the Wine
... by osmotic stress (Rep et al. 1999). IPP1 was also expressed at the same levels in ST and VIN7. Samples were cycled 40 times between 95°C for 15 sec and 57°C for 1 min. To cal‑ culate the fold change, the CT value of IPP1 was subtracted from the gene of interest in the same cDNA sample (e.g., GeneX ...
... by osmotic stress (Rep et al. 1999). IPP1 was also expressed at the same levels in ST and VIN7. Samples were cycled 40 times between 95°C for 15 sec and 57°C for 1 min. To cal‑ culate the fold change, the CT value of IPP1 was subtracted from the gene of interest in the same cDNA sample (e.g., GeneX ...
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... pulmonary obstruction measurement and one of the most significant predictors for chronic obstructive pulmonary disease (COPD), which is a heritable multi-factorial disease. We present genome-wide association study (GWAS) to map the genetic architecture of this trait and investigate the networks betw ...
... pulmonary obstruction measurement and one of the most significant predictors for chronic obstructive pulmonary disease (COPD), which is a heritable multi-factorial disease. We present genome-wide association study (GWAS) to map the genetic architecture of this trait and investigate the networks betw ...
PDF
... and polarized light sensors (Hardie, 1985). According to their differentiated function, R1-6 innervate the first OL neuropil, which is the lamina, whereas R7 and R8 axons run across the lamina to innervate the neuropil beneath, which is the medulla. Next, the lobula and lobula plate (together called ...
... and polarized light sensors (Hardie, 1985). According to their differentiated function, R1-6 innervate the first OL neuropil, which is the lamina, whereas R7 and R8 axons run across the lamina to innervate the neuropil beneath, which is the medulla. Next, the lobula and lobula plate (together called ...
Genes involved in asexual sporophyte development in Ceratopteris
... bypass meiosis and fertilization, have evolved in many plants representing diverse taxa among the land plant phylogeny. Apogamy is an asexual reproductive strategy that produces a sporophyte directly from a gametophyte cell without the usual ploidy change associated with syngamy. The genes that driv ...
... bypass meiosis and fertilization, have evolved in many plants representing diverse taxa among the land plant phylogeny. Apogamy is an asexual reproductive strategy that produces a sporophyte directly from a gametophyte cell without the usual ploidy change associated with syngamy. The genes that driv ...
4 Mapping Eukaryotic Chromosomes by
... a. In Figure 4-21, let GC = A and AT = a, then draw the fungal octad that would result from the final structure (5). b. (Challenging) Insert some closely linked flanking markers into the diagram, say P/p to the left and Q/q to the right (assume either cis or trans arrangements). Assume neither of th ...
... a. In Figure 4-21, let GC = A and AT = a, then draw the fungal octad that would result from the final structure (5). b. (Challenging) Insert some closely linked flanking markers into the diagram, say P/p to the left and Q/q to the right (assume either cis or trans arrangements). Assume neither of th ...
mRNA Export - e
... simultaneous knock down of Aly/REF and THOC5 block the nuclear export of mRNA Taken together, these data suggest that, at least in mammalian cells, the TREX component Thoc5 could be the factor that cooperates with Aly/REF. Recent studies indicated that the nuclear export of only a subset of genes is ...
... simultaneous knock down of Aly/REF and THOC5 block the nuclear export of mRNA Taken together, these data suggest that, at least in mammalian cells, the TREX component Thoc5 could be the factor that cooperates with Aly/REF. Recent studies indicated that the nuclear export of only a subset of genes is ...
GROW`N`GLOW: THE ACE1 TWO-HYBRID
... has proven to be a powerful tool for identifying proteins from an expression library which can interact with one's protein of interest. The technology is based on the fact that many eukaryotic transcriptional activators, such as GAL4, consist of two physically separable modular domains, one DNA-bind ...
... has proven to be a powerful tool for identifying proteins from an expression library which can interact with one's protein of interest. The technology is based on the fact that many eukaryotic transcriptional activators, such as GAL4, consist of two physically separable modular domains, one DNA-bind ...
Ch15 ppt - WEB . WHRSD . ORG
... most traits are controlled by a single gene each gene has only 2 alleles, 1 of which is completely dominant to the other ...
... most traits are controlled by a single gene each gene has only 2 alleles, 1 of which is completely dominant to the other ...
Hormone Autotrophic Growth and Differentiation
... sensitivity of certain tissues to cytokinins or activates growth-factor-independent pathways. Other examples of genes that deregulate proper control of cell division and growth are the oncogenes 6b and lso of A. tumefaciens T-DNA. Infection with either gene leads to the formation of undifferentiated ...
... sensitivity of certain tissues to cytokinins or activates growth-factor-independent pathways. Other examples of genes that deregulate proper control of cell division and growth are the oncogenes 6b and lso of A. tumefaciens T-DNA. Infection with either gene leads to the formation of undifferentiated ...
Chapter 14.
... most traits are controlled by a single gene each gene has only 2 alleles, 1 of which is completely dominant to the other ...
... most traits are controlled by a single gene each gene has only 2 alleles, 1 of which is completely dominant to the other ...
Comparative analysis of two-component signal transduction systems
... that end, two bootstrapped NJ trees were constructed, a HK tree and a RR tree. The HK tree was constructed with all B. cereus group and B. subtilis HK phosphotransferase domains, while the RR tree was constructed with all RR receiver domains. In addition to this initial set of sequences, homologous ...
... that end, two bootstrapped NJ trees were constructed, a HK tree and a RR tree. The HK tree was constructed with all B. cereus group and B. subtilis HK phosphotransferase domains, while the RR tree was constructed with all RR receiver domains. In addition to this initial set of sequences, homologous ...
Monday, November 17, 2014 Agenda: Cell Organelle Analogy
... • Intimate contact provides route for infection by parasites (AIDS, syphillis, etc.) • Genetic costs: in sex, we pass on only half of genes to offspring. • Males are an expensive luxury - in most species they contribute little to rearing offspring. ...
... • Intimate contact provides route for infection by parasites (AIDS, syphillis, etc.) • Genetic costs: in sex, we pass on only half of genes to offspring. • Males are an expensive luxury - in most species they contribute little to rearing offspring. ...
PDF
... of auxin signaling (Guilfoyle and Hagen, 2007; Guilfoyle, 2015). Most of the Aux/IAA proteins contain four conserved domains, namely, Domain I, Domain II, Domain III, and Domain IV. Domain I is an active repression domain containing a conserved LxLxL motif. Domain II contains a conserved degron and ...
... of auxin signaling (Guilfoyle and Hagen, 2007; Guilfoyle, 2015). Most of the Aux/IAA proteins contain four conserved domains, namely, Domain I, Domain II, Domain III, and Domain IV. Domain I is an active repression domain containing a conserved LxLxL motif. Domain II contains a conserved degron and ...
Jigsaw handout - the Biology Scholars Program Wiki
... binds to lac repressor and causes a conformation change that alters the structure of lac repressor so that it cannot bind to DNA. Therefore, in the presence of lactose, lac repressor does not bind to the operator and RNA polymerase can transcribe the genes in the lac operon. Case study (homework or ...
... binds to lac repressor and causes a conformation change that alters the structure of lac repressor so that it cannot bind to DNA. Therefore, in the presence of lactose, lac repressor does not bind to the operator and RNA polymerase can transcribe the genes in the lac operon. Case study (homework or ...
The DUET gene is necessary for chromosome
... (Siddiqi et al., 2000). A 1.26 kb coding region of DUET cDNA lacking the PHD-finger domain was amplified using the primers STMF1 (5′-GGTCATTTGGTATGTGTCAATGGTATGG-3′) and STMR3 (5′-TCAATCTCAGTCACTACAAAATTTGACAAG-3′) and subcloned into pGEM-T (Promega) for synthesis of RNA probes. Molecular analysis o ...
... (Siddiqi et al., 2000). A 1.26 kb coding region of DUET cDNA lacking the PHD-finger domain was amplified using the primers STMF1 (5′-GGTCATTTGGTATGTGTCAATGGTATGG-3′) and STMR3 (5′-TCAATCTCAGTCACTACAAAATTTGACAAG-3′) and subcloned into pGEM-T (Promega) for synthesis of RNA probes. Molecular analysis o ...
Report Number of Nuclear Divisions in the Drosophila Blastoderm
... divisions, comparable to uninjected X161 embryos (Figure 4B and Table 2). This experiment demonstrates that the reduced division number in X161 embryos requires zygotic gene expression. The expression of many early zygotic genes is controlled by the zinc-finger protein Vfl (also called Zelda) [13]. ...
... divisions, comparable to uninjected X161 embryos (Figure 4B and Table 2). This experiment demonstrates that the reduced division number in X161 embryos requires zygotic gene expression. The expression of many early zygotic genes is controlled by the zinc-finger protein Vfl (also called Zelda) [13]. ...