Engineering Acetyl Coenzyme A Supply: Functional Expression of a
... subunits presumably have no mitochondrial localization sequences; E. faecalis PDH is relatively insensitive to high NADH/ NAD⫹ ratios, which allows it to function under anaerobic as well as aerobic conditions in its natural host; the E. faecalis PDH complex has been shown to self-assemble from purif ...
... subunits presumably have no mitochondrial localization sequences; E. faecalis PDH is relatively insensitive to high NADH/ NAD⫹ ratios, which allows it to function under anaerobic as well as aerobic conditions in its natural host; the E. faecalis PDH complex has been shown to self-assemble from purif ...
ETO-Thesis-Final 061214. - JScholarship
... There are multiple routes for transmission of HIV-1, but the most prevalent is through vaginal or anal intercourse [1]. Through efforts including male circumcision and increased condom use, the amount of new human immunodeficiency virus (HIV) infections decreased from 3.4 million in 2001 to 2.3 mill ...
... There are multiple routes for transmission of HIV-1, but the most prevalent is through vaginal or anal intercourse [1]. Through efforts including male circumcision and increased condom use, the amount of new human immunodeficiency virus (HIV) infections decreased from 3.4 million in 2001 to 2.3 mill ...
ISOLATION AND FUNCTIONAL GENETIC EUCALYPTUS Magister Scientiae
... world-wide demand. The pulp and papermaking industry is also a large source of pollution mainly due to the use of many toxic chemicals when wood fibre is processed. The amount of chemicals required for pulping can be reduced by altering wood properties such as lignin content and composition. Paper q ...
... world-wide demand. The pulp and papermaking industry is also a large source of pollution mainly due to the use of many toxic chemicals when wood fibre is processed. The amount of chemicals required for pulping can be reduced by altering wood properties such as lignin content and composition. Paper q ...
Absence of translationally selected synonymous codon usage
... used for all amino acids (where there is a choice) in all genes (Andersson & Sharp, 1996a ; Ohkubo et al., 1987). Generally, in these species there is little heterogeneity among genes ; all feature similar extremely biased codon usage. However, two types of intragenomic variation in codon usage rela ...
... used for all amino acids (where there is a choice) in all genes (Andersson & Sharp, 1996a ; Ohkubo et al., 1987). Generally, in these species there is little heterogeneity among genes ; all feature similar extremely biased codon usage. However, two types of intragenomic variation in codon usage rela ...
GPR91: expanding the frontiers of Krebs cycle intermediates
... These amino acids are all located in helices and agglomerate in the central area of the receptor - a rhodopsinlike structure - in a way that the positive charge attracts succinate [11]. We now show the result of an in-silico study of GRP91 and its possible succinate-binding site (Fig. 2). Although G ...
... These amino acids are all located in helices and agglomerate in the central area of the receptor - a rhodopsinlike structure - in a way that the positive charge attracts succinate [11]. We now show the result of an in-silico study of GRP91 and its possible succinate-binding site (Fig. 2). Although G ...
The Nrf2/ARE Pathway as a Potential Therapeutic Target in
... from systemic 3-NP administration. Nrf2-deficient mice receiving 3-NP (50 mg/kg every 12 h for seven injections) performed poorly on the rotarod task (Fig. 2C) and lost an average of 12% of their starting weight over the course of 4 days (7). Furthermore, measurable striatal lesions were present in ...
... from systemic 3-NP administration. Nrf2-deficient mice receiving 3-NP (50 mg/kg every 12 h for seven injections) performed poorly on the rotarod task (Fig. 2C) and lost an average of 12% of their starting weight over the course of 4 days (7). Furthermore, measurable striatal lesions were present in ...
A PEST-like Sequence in the N-Terminal Cytoplasmic Domain of
... MAL61. A mal61/HA null derivative of CMY1001 was constructed by PCR-based gene disruption as follows (15). The upstream (5′) primer consists of 45 bases of sequence complementary to the upstream region of MAL61/HA, followed by 19 bases of sequence complementary to the HIS3 gene 5′ sequence. Its sequ ...
... MAL61. A mal61/HA null derivative of CMY1001 was constructed by PCR-based gene disruption as follows (15). The upstream (5′) primer consists of 45 bases of sequence complementary to the upstream region of MAL61/HA, followed by 19 bases of sequence complementary to the HIS3 gene 5′ sequence. Its sequ ...
AMP-activated protein kinase phosphorylates transcription factors of
... promoter region of the hexokinase II gene, we hypothesized that the cAMP-response element (CRE) binding protein (CREB) family of transcription factors could be targets of AMPK. Using radioisotopic kinase assays, we found that recombinant and rat liver and muscle AMPK phosphorylated CREB1 at the same ...
... promoter region of the hexokinase II gene, we hypothesized that the cAMP-response element (CRE) binding protein (CREB) family of transcription factors could be targets of AMPK. Using radioisotopic kinase assays, we found that recombinant and rat liver and muscle AMPK phosphorylated CREB1 at the same ...
Short- and long-range functions of Goosecoid in
... in the late blastula/early gastrula by regulating the expression of the transcriptional repressors Vox and Vent, whereas BMP signaling is required to maintain expression of these genes during late gastrulation (Ramel et al., 2005; Ramel and Lekven, 2004). Thus, Wnt and BMP act together to limit the ...
... in the late blastula/early gastrula by regulating the expression of the transcriptional repressors Vox and Vent, whereas BMP signaling is required to maintain expression of these genes during late gastrulation (Ramel et al., 2005; Ramel and Lekven, 2004). Thus, Wnt and BMP act together to limit the ...
The Role of the Bundle Sheath in the Leaf Development of C3 plants
... step of de novo purine biosynthesis (Manuscript 2). Enzyme activity tests demonstrated lowered activity for the mutated protein. The purine-derived cytokinins, phytohormones promoting plant growth, are present at lower levels in the mutant. Cytokinin feeding and hormone reporter assays showed that l ...
... step of de novo purine biosynthesis (Manuscript 2). Enzyme activity tests demonstrated lowered activity for the mutated protein. The purine-derived cytokinins, phytohormones promoting plant growth, are present at lower levels in the mutant. Cytokinin feeding and hormone reporter assays showed that l ...
Regulation of 7-Dehydrocholesterol Reductase by Vitamin D3
... It has been well established that DHCR7 is regulated by the SREBP pathway in the regulation of cholesterol synthesis, but little is known about the regulation of DHCR7 by the vitamin D pathway. In this study, the regulation of DHCR7 activity by vitamin D was explored. Treatment of adult human epider ...
... It has been well established that DHCR7 is regulated by the SREBP pathway in the regulation of cholesterol synthesis, but little is known about the regulation of DHCR7 by the vitamin D pathway. In this study, the regulation of DHCR7 activity by vitamin D was explored. Treatment of adult human epider ...
Identification and Analysis of Dicer Associated Proteins in
... Dicer’s N-terminal helicase domain and TRBP’s third dsRBD. TRBP has previously been assigned several different functions, including inhibition of the interferon (IFN)-induced dsRNA-regulated protein kinase PKR, modulation of ...
... Dicer’s N-terminal helicase domain and TRBP’s third dsRBD. TRBP has previously been assigned several different functions, including inhibition of the interferon (IFN)-induced dsRNA-regulated protein kinase PKR, modulation of ...
Engineering Cytosolic Acetyl-CoA Metabolism in Saccharomyces
... A Saccharomyces cerevisiae strain carrying deletions in all three pyruvate decarboxylase genes (also called Pdc negative yeast) represents a non-ethanol producing platform strain for biochemical production. However, it cannot grow on glucose as the sole carbon source due to the lack of cytosolic ace ...
... A Saccharomyces cerevisiae strain carrying deletions in all three pyruvate decarboxylase genes (also called Pdc negative yeast) represents a non-ethanol producing platform strain for biochemical production. However, it cannot grow on glucose as the sole carbon source due to the lack of cytosolic ace ...
Gene Section FST (follistatin) Atlas of Genetics and Cytogenetics
... bound activin is unable to initiate signal transduction and consequently follistatin is a potent antagonist of physiological activin signals. Of the three follistatin domains present in all follistatin isoforms, (Shimasaki et al., 1988) the first two, but not the third, are necessary for activin A b ...
... bound activin is unable to initiate signal transduction and consequently follistatin is a potent antagonist of physiological activin signals. Of the three follistatin domains present in all follistatin isoforms, (Shimasaki et al., 1988) the first two, but not the third, are necessary for activin A b ...
Lactococcus lactis as expression host for the biosynthetic
... in CDM. In this case, the growth was partially restored after the addition of Trp. Glyphosate inhibits the function of the EPSP (enzyme 5-enolpyruvylshikimate-3-phosphate synthase) [19], which acts upstream in the formation of chorismate, a precursor of all aromatic amino acids. Growth inhibition ma ...
... in CDM. In this case, the growth was partially restored after the addition of Trp. Glyphosate inhibits the function of the EPSP (enzyme 5-enolpyruvylshikimate-3-phosphate synthase) [19], which acts upstream in the formation of chorismate, a precursor of all aromatic amino acids. Growth inhibition ma ...
Role of CD3 /in T Cell Receptor Assembly
... have shown that glycosylation might be involved in stabilizing the structure of proteins, in modifying the functional activity of proteins, in recognition events, and in signal transduction after ligand-receptor binding (reviewed in reference 53). In addition, it has recently been demonstrated that ...
... have shown that glycosylation might be involved in stabilizing the structure of proteins, in modifying the functional activity of proteins, in recognition events, and in signal transduction after ligand-receptor binding (reviewed in reference 53). In addition, it has recently been demonstrated that ...
PPT
... •Using perturbagen responses to map signaling pathways in normal and diseased liver: Collaboration with Vertex. ...
... •Using perturbagen responses to map signaling pathways in normal and diseased liver: Collaboration with Vertex. ...
Transcriptome analysis of the two unrelated fungal
... each strain were obtained from two independent biological replicates and analysis was performed using DEseq2 [42]. We chose culture conditions that were previously found to be optimal for measuring the activity of β-lactam antibiotic biosynthesis genes [26, 30, 43–45]. Therefore, we grew all strains ...
... each strain were obtained from two independent biological replicates and analysis was performed using DEseq2 [42]. We chose culture conditions that were previously found to be optimal for measuring the activity of β-lactam antibiotic biosynthesis genes [26, 30, 43–45]. Therefore, we grew all strains ...
709_2010_211_MOESM2_ESM - Springer Static Content Server
... pattern and is involved in vascular strand development in the leaf, control of leaf shape, size and symmetry, male gametophyte development and ovule specification and function. Anthers of double mutants (bam1bam2) appeared abnormal at a very early stage and lack the endothecium, middle, and tapetum ...
... pattern and is involved in vascular strand development in the leaf, control of leaf shape, size and symmetry, male gametophyte development and ovule specification and function. Anthers of double mutants (bam1bam2) appeared abnormal at a very early stage and lack the endothecium, middle, and tapetum ...
Summary
... nasopharyngeal brushings. The study included a total of 217 individuals with various head and neck complaints selected during year 2007-2010 and demonstrated a 34.6% seroprevalence based on EBV IgA serology. After IgG immunoblot and viral load confirmation, further clinical investigation identified ...
... nasopharyngeal brushings. The study included a total of 217 individuals with various head and neck complaints selected during year 2007-2010 and demonstrated a 34.6% seroprevalence based on EBV IgA serology. After IgG immunoblot and viral load confirmation, further clinical investigation identified ...
Identification of Functional Connections Between Calmodulin and the Yeast Actin Cytoskeleton.
... Botstein 1994a). Direct binding between calmodulin and actin had not been detected in a gel overlay assay or by sedimenting F-actin in the presence of calmodulin (Piazza and Wallace 1985), suggesting that the phenotypes of cmd1-226 must be an indirect effect mediated by an as yet unidentified actin- ...
... Botstein 1994a). Direct binding between calmodulin and actin had not been detected in a gel overlay assay or by sedimenting F-actin in the presence of calmodulin (Piazza and Wallace 1985), suggesting that the phenotypes of cmd1-226 must be an indirect effect mediated by an as yet unidentified actin- ...
Cells Phenotype of Human Tolerogenic Dendritic Glycolytic
... Analysis of DC surface marker expression showed that HLA-DR (MHC class II), CD83, and CD86 expression levels in tolerogenic moDCs were similar to immature moDCs. Furthermore, tolerogenic moDCs exhibited a resistance to maturation following LPS stimulation, compared with mature moDCs, which had high ...
... Analysis of DC surface marker expression showed that HLA-DR (MHC class II), CD83, and CD86 expression levels in tolerogenic moDCs were similar to immature moDCs. Furthermore, tolerogenic moDCs exhibited a resistance to maturation following LPS stimulation, compared with mature moDCs, which had high ...
Trade-offs between tRNA abundance and mRNA secondary
... Translation of mRNAs into protein is crucial for cell viability and function and proceeds at a non-uniform rate along transcripts (1). While much focus has been placed on the translation initiation step that is often rate limiting for endogenous genes (2,3), there is growing realization that the var ...
... Translation of mRNAs into protein is crucial for cell viability and function and proceeds at a non-uniform rate along transcripts (1). While much focus has been placed on the translation initiation step that is often rate limiting for endogenous genes (2,3), there is growing realization that the var ...
RNA interference - Bio
... (PTGS) in plants1 and later independently identified in wide variety of eukaryotic organisms. These dsRNAs are rapidly processed into short RNA duplexes of 21 to 28 nucleotides in length, which then guide the recognition and ultimately the cleavage of complementary single-stranded RNAs, such as mess ...
... (PTGS) in plants1 and later independently identified in wide variety of eukaryotic organisms. These dsRNAs are rapidly processed into short RNA duplexes of 21 to 28 nucleotides in length, which then guide the recognition and ultimately the cleavage of complementary single-stranded RNAs, such as mess ...