Pathways to abscisic acid-regulated gene expression
... similar to the amino-termini of ARF1, a transcription factor that binds to auxin response elements (Ulmasov et al., 1997), the ARF1-like MONOPTEROS gene involved in embryo axis formation and vascular development (Table 1) (Hardtke & Berleth, 1998), and several Arabidopsis DNA-binding proteins of unk ...
... similar to the amino-termini of ARF1, a transcription factor that binds to auxin response elements (Ulmasov et al., 1997), the ARF1-like MONOPTEROS gene involved in embryo axis formation and vascular development (Table 1) (Hardtke & Berleth, 1998), and several Arabidopsis DNA-binding proteins of unk ...
Pentose phosphates in nucleoside interconversion and catabolism
... During the course of experiments designed to isolate deoxyRib-1-P formed by the reversible enzymatic phosphorolysis of deoxyguanosine catalysed by PNP, in 1952 Friedkin tried to increase the yield of deoxyRib-1-P by coupling deoxyguanosine phosphorolysis with the irreversible guanine deamination, ca ...
... During the course of experiments designed to isolate deoxyRib-1-P formed by the reversible enzymatic phosphorolysis of deoxyguanosine catalysed by PNP, in 1952 Friedkin tried to increase the yield of deoxyRib-1-P by coupling deoxyguanosine phosphorolysis with the irreversible guanine deamination, ca ...
Activation of Peroxisome Proliferator-Activated Receptors
... agent in dry cleaning, paint stripping, and health effects, including liver, kidney, and at high doses. This response is accompanied industrial cleaning that is of particular central nervous system toxicity (2). The tox- by a substantial increase in peroxisomal interest to Superfund deanup efforts. ...
... agent in dry cleaning, paint stripping, and health effects, including liver, kidney, and at high doses. This response is accompanied industrial cleaning that is of particular central nervous system toxicity (2). The tox- by a substantial increase in peroxisomal interest to Superfund deanup efforts. ...
Vaccinia Virus G1L Protein Expression and Purification
... ymxG (Hypothetical zinc protease from B. subtilis) ...
... ymxG (Hypothetical zinc protease from B. subtilis) ...
A non-canonical pathway for aromatic amino acid biosynthesis in
... Expression and purification of OE1472F, OE2019F & OE1477R ............ 100 Expression of OE1472F in H. salinarum......................................................... 100 ...
... Expression and purification of OE1472F, OE2019F & OE1477R ............ 100 Expression of OE1472F in H. salinarum......................................................... 100 ...
Desert hedgehog is a mammal-specific gene expressed during testicular and ovarian
... and in the adult ovaries where Ihh and Dhh are coexpressed [11,12]. Shh has an essential role in early fetal development, and is required for correct formation of the limbs, phallus, somites and neural tube [8-10]. Ihh has a more restricted developmental role, and is essential for chondrocyte develo ...
... and in the adult ovaries where Ihh and Dhh are coexpressed [11,12]. Shh has an essential role in early fetal development, and is required for correct formation of the limbs, phallus, somites and neural tube [8-10]. Ihh has a more restricted developmental role, and is essential for chondrocyte develo ...
Quantitative RT-PCR Platform to Measure Transcript Levels of C and
... down-regulated the photosynthetic capacity and led to the loss of N compounds, including Rubisco; ...
... down-regulated the photosynthetic capacity and led to the loss of N compounds, including Rubisco; ...
Desert hedgehogis a mammal-specific gene expressed during
... and in the adult ovaries where Ihh and Dhh are coexpressed [11,12]. Shh has an essential role in early fetal development, and is required for correct formation of the limbs, phallus, somites and neural tube [8-10]. Ihh has a more restricted developmental role, and is essential for chondrocyte develo ...
... and in the adult ovaries where Ihh and Dhh are coexpressed [11,12]. Shh has an essential role in early fetal development, and is required for correct formation of the limbs, phallus, somites and neural tube [8-10]. Ihh has a more restricted developmental role, and is essential for chondrocyte develo ...
Molecular Cloning and Expression Analysis of IgD in Nile
... Abstract: IgD is considered to be a recently-evolved Ig and a puzzling molecule, being previously found in all vertebrate taxa, except for birds. Although IgD likely plays an important role in vertebrate immune responses, the function of IgD in Nile tilapia (Oreochromis niloticus) is virtually unkno ...
... Abstract: IgD is considered to be a recently-evolved Ig and a puzzling molecule, being previously found in all vertebrate taxa, except for birds. Although IgD likely plays an important role in vertebrate immune responses, the function of IgD in Nile tilapia (Oreochromis niloticus) is virtually unkno ...
Coordination of the maize transcriptome by a conserved circadian
... Conclusions: Circadian regulation of the maize genome was widespread and key genes in several major metabolic pathways had circadian expression waveforms. The maize circadian clock coordinated transcription to be coincident with oncoming day or night, which was consistent with the circadian oscillat ...
... Conclusions: Circadian regulation of the maize genome was widespread and key genes in several major metabolic pathways had circadian expression waveforms. The maize circadian clock coordinated transcription to be coincident with oncoming day or night, which was consistent with the circadian oscillat ...
Computational Biology - Bioinformatik
... depending on the cell type and pattern of gene expression. Approximately 50% of all annotated human miRNA genes are located in fragile sites or areas of the genome that are associated with cancer. → Mutations in miRNAs or their binding sites may lead to diseases. E.g. Abelson et al. found that a mut ...
... depending on the cell type and pattern of gene expression. Approximately 50% of all annotated human miRNA genes are located in fragile sites or areas of the genome that are associated with cancer. → Mutations in miRNAs or their binding sites may lead to diseases. E.g. Abelson et al. found that a mut ...
Expression of the LXR Protein in Human Atherosclerotic Lesions
... also stained the lesion with anti–SR-A and anti-CD68 antibodies. Figure 6G indicates that the LXR␣ and SR-A proteins were detected in the same cells. The SR-A protein (purple) was mainly detected in association with the cell membrane, and the LXR␣ protein (brown) was detected mainly in the nucleus. ...
... also stained the lesion with anti–SR-A and anti-CD68 antibodies. Figure 6G indicates that the LXR␣ and SR-A proteins were detected in the same cells. The SR-A protein (purple) was mainly detected in association with the cell membrane, and the LXR␣ protein (brown) was detected mainly in the nucleus. ...
Open the publication - UEF Electronic Publications
... drastically affects bodily functions, such as behavior, metabolism and body temperature16–21. The disruptions of the system have been linked to variety of diseases, including obesity, diabetes, certain cancers and mental problems, such as depression and schizophrenia16,19. Pgc1α, key regulator of ce ...
... drastically affects bodily functions, such as behavior, metabolism and body temperature16–21. The disruptions of the system have been linked to variety of diseases, including obesity, diabetes, certain cancers and mental problems, such as depression and schizophrenia16,19. Pgc1α, key regulator of ce ...
VEGF and endochondral bone formation
... chondrocyte differentation gave evidence supporting this hypothesis (Descalzi et al., 1995): single hypertrophic chondrocytes in suspension have inhibitory effects on endothelial cell migration and invasion; when supplemented with ascorbic acid, a condition that allows organization of an extracellul ...
... chondrocyte differentation gave evidence supporting this hypothesis (Descalzi et al., 1995): single hypertrophic chondrocytes in suspension have inhibitory effects on endothelial cell migration and invasion; when supplemented with ascorbic acid, a condition that allows organization of an extracellul ...
Retina-Specific Expression of 5A11/Basigin-2, a
... ecently, 5A11/Basigin, a member of the immunoglobulin gene superfamily, has emerged as an important glycoprotein in the mouse retina. Mice in which the gene for 5A11/ Basigin has been inactivated are visually impaired from the time of eye opening (2 weeks of age) through maturity, as indicated by ER ...
... ecently, 5A11/Basigin, a member of the immunoglobulin gene superfamily, has emerged as an important glycoprotein in the mouse retina. Mice in which the gene for 5A11/ Basigin has been inactivated are visually impaired from the time of eye opening (2 weeks of age) through maturity, as indicated by ER ...
Isolated Spinach Ribulose-1,5-Bisphosphate
... transgenic tobacco plants,” Proc. Nat. Acad. Sci. USA 89:8394—8398 (1992)). These studies have been inconclu ...
... transgenic tobacco plants,” Proc. Nat. Acad. Sci. USA 89:8394—8398 (1992)). These studies have been inconclu ...
Computational disease gene identification
... plays an important role (12), it is clear that both pre- and post-natal environment and lifestyle are critical contributors to diabetes pathogenesis (13). Several relatively uncommon monogenic forms of diabetes have been characterized. These frequently entail defects in pancreatic b-cells and insuli ...
... plays an important role (12), it is clear that both pre- and post-natal environment and lifestyle are critical contributors to diabetes pathogenesis (13). Several relatively uncommon monogenic forms of diabetes have been characterized. These frequently entail defects in pancreatic b-cells and insuli ...
Design considerations for highly specific and efficient
... In order to understand the parameters affecting CRISPR-Cas9 gene editing efficiency, we systematically transfected synthetic CRISPR RNA (crRNA) and trans-activating CRISPR RNA (tracrRNA) reagents targeting components of the proteasome into a reporter cell line in which knockout of proteasome functio ...
... In order to understand the parameters affecting CRISPR-Cas9 gene editing efficiency, we systematically transfected synthetic CRISPR RNA (crRNA) and trans-activating CRISPR RNA (tracrRNA) reagents targeting components of the proteasome into a reporter cell line in which knockout of proteasome functio ...
Counting Small RNA in Pathogenic Bacteria
... typically between 0 and 10 transcripts. The numbers of these RNA are both increased (by a factor of 2.5× for YSR35 and 3.5× for YSP8) upon a temperature shift from 25 to 37 °C, suggesting they play a role in pathogenesis. The copy number distribution of sRNAs from bacteria-to-bacteria are well-fit wi ...
... typically between 0 and 10 transcripts. The numbers of these RNA are both increased (by a factor of 2.5× for YSR35 and 3.5× for YSP8) upon a temperature shift from 25 to 37 °C, suggesting they play a role in pathogenesis. The copy number distribution of sRNAs from bacteria-to-bacteria are well-fit wi ...
In Vitro Characterization of Human Growth Hormone
... Growth hormone (GH) is a 22 kD, 191-aa, pituitary-derived peptide hormone that is essential for postnatal growth. GH signals via binding to GH receptor (GHR), which initiates intracellular signal transduction pathways. This leads to activation of target genes, most importantly the one encoding insul ...
... Growth hormone (GH) is a 22 kD, 191-aa, pituitary-derived peptide hormone that is essential for postnatal growth. GH signals via binding to GH receptor (GHR), which initiates intracellular signal transduction pathways. This leads to activation of target genes, most importantly the one encoding insul ...
nitrogen assimilation and global regulation in escherichia coli
... would normally generate glutamate independent of both GOGAT and GDH (e.g., arginine catabolism). Alternatively, the glutamate-starvation hypothesis proposes that glutamate starvation prevents Ntr induction (35). A mechanism by which this occurs is not stated. A hybrid hypothesis is that glutamine ac ...
... would normally generate glutamate independent of both GOGAT and GDH (e.g., arginine catabolism). Alternatively, the glutamate-starvation hypothesis proposes that glutamate starvation prevents Ntr induction (35). A mechanism by which this occurs is not stated. A hybrid hypothesis is that glutamine ac ...
Glutamine and cancer: cell biology, physiology
... glutamine in culture (8). Glutamine catabolism begins with its conversion to glutamate in reactions that either donate the amide nitrogen to biosynthetic pathways or release it as ammonia. The latter reactions are catalyzed by the glutaminases (GLSs), of which several isozymes are encoded by human g ...
... glutamine in culture (8). Glutamine catabolism begins with its conversion to glutamate in reactions that either donate the amide nitrogen to biosynthetic pathways or release it as ammonia. The latter reactions are catalyzed by the glutaminases (GLSs), of which several isozymes are encoded by human g ...
Differential recruitment of Dishevelled provides signaling specificity
... homolog of b-catenin), destabilizing the cytoplasmic form of the protein. In the presence of Wg, the receptor acts through Dsh to antagonize Zw3 activity, and Arm is stabilized, resulting in its interaction with Pangolin (Pan, a TCF/Lef1-type transcription factor), forming a stable transcriptional r ...
... homolog of b-catenin), destabilizing the cytoplasmic form of the protein. In the presence of Wg, the receptor acts through Dsh to antagonize Zw3 activity, and Arm is stabilized, resulting in its interaction with Pangolin (Pan, a TCF/Lef1-type transcription factor), forming a stable transcriptional r ...
Two glucose/xylose transporter genes from the yeast Candida
... observed for the original library clone (results not shown). One of these transformants (strain MJY1) was selected for further characterization. Subcloning and subsequent sequencing revealed the presence of an ORF encoding a putative membrane protein of 547 amino acids. Similarity and domain searche ...
... observed for the original library clone (results not shown). One of these transformants (strain MJY1) was selected for further characterization. Subcloning and subsequent sequencing revealed the presence of an ORF encoding a putative membrane protein of 547 amino acids. Similarity and domain searche ...
PPT - Altogen Biosystems
... 5. Optional: Add 2 µl of Complex Condenser. This reagent reduces the size of transfection complex, therefore increasing transfection efficiency; however it may increase cell toxicity 6. Add prepared transfection complexes to 0.5 ml of complete growth medium with ARPE-19 cells (from step ...
... 5. Optional: Add 2 µl of Complex Condenser. This reagent reduces the size of transfection complex, therefore increasing transfection efficiency; however it may increase cell toxicity 6. Add prepared transfection complexes to 0.5 ml of complete growth medium with ARPE-19 cells (from step ...