evidence indicating independent assortment of
... also show that these nucleotides code only for a portion of a domain and therefore constitute a minigene. It has been suggested (22, 23) that it evolved from a complete domain by a shift of a splice site followed by mutational divergence of the left end of the domain to become a portion of an interv ...
... also show that these nucleotides code only for a portion of a domain and therefore constitute a minigene. It has been suggested (22, 23) that it evolved from a complete domain by a shift of a splice site followed by mutational divergence of the left end of the domain to become a portion of an interv ...
Differential mRNA expression levels and gene sequences of a
... of activity against α-naphthyl acetate. We are currently attempting to purify the MCE but are also interested in characterizing the molecular basis of the resistance at the gene level. In this paper we describe the full sequence of carboxylesterase-like enzyme cDNA clones from both malathion-resista ...
... of activity against α-naphthyl acetate. We are currently attempting to purify the MCE but are also interested in characterizing the molecular basis of the resistance at the gene level. In this paper we describe the full sequence of carboxylesterase-like enzyme cDNA clones from both malathion-resista ...
Nucleic Acids Research
... mitochondria of all eucaryotic cells. It is one of a series of proteins involved in the electron transport chain, and is therefore required for aerobic respiration. Due to structural and functional constraints, the cytochrome c proteins of widely divergent organisms exhibit considerable amino acid h ...
... mitochondria of all eucaryotic cells. It is one of a series of proteins involved in the electron transport chain, and is therefore required for aerobic respiration. Due to structural and functional constraints, the cytochrome c proteins of widely divergent organisms exhibit considerable amino acid h ...
User`s Guide - Structural Bioinformatics Group
... header and click Tools ClustalW. As usual, the preferences window will appear allowing you to modify some of the most important parameters of the algorithm. We can just keep values at their default and submit. A dialog box will appear asking if we want to keep the previously obtained structural al ...
... header and click Tools ClustalW. As usual, the preferences window will appear allowing you to modify some of the most important parameters of the algorithm. We can just keep values at their default and submit. A dialog box will appear asking if we want to keep the previously obtained structural al ...
Identification of genes from flat oyster Ostrea
... they must be carefully validated as pointed out in the MIQE guideline. This guideline suggests a minimum set of information that researcher should provide for their quantitative real time PCR data [8]. The ideal housekeeping gene should present a stable mRNA expression and should be minimally regula ...
... they must be carefully validated as pointed out in the MIQE guideline. This guideline suggests a minimum set of information that researcher should provide for their quantitative real time PCR data [8]. The ideal housekeeping gene should present a stable mRNA expression and should be minimally regula ...
blast
... • DNA sequences contain less information with which to deduce homology than do the encoded protein sequences when compared using simple nucleotide substitution scores, 20 aa vs 4 nt! • Protein comparisons give more meaningful results • Moderately similar nt sequences often a highly similar protein s ...
... • DNA sequences contain less information with which to deduce homology than do the encoded protein sequences when compared using simple nucleotide substitution scores, 20 aa vs 4 nt! • Protein comparisons give more meaningful results • Moderately similar nt sequences often a highly similar protein s ...
The pyruvate dehydrogenase complex of the chemol
... Bglll; E, EcoRI; H, Hindlll; K, Kpnl; 51, Sad; SII, Sacll; and Sm, Smal. ...
... Bglll; E, EcoRI; H, Hindlll; K, Kpnl; 51, Sad; SII, Sacll; and Sm, Smal. ...
Molecular cloning and nucleotide sequence of another variant of the
... cytotoxicity to HeLa cells compared with Vero cells, it is appropriateto consider both toxins as variants of SLTII. SLTIIva M e r s from SLTIIv in that it is more heat-stable. Further, SLTIIv-producingstrains of E. cofi have only been isolated from pigs while the SLTIIva-producingE. coli strain exam ...
... cytotoxicity to HeLa cells compared with Vero cells, it is appropriateto consider both toxins as variants of SLTII. SLTIIva M e r s from SLTIIv in that it is more heat-stable. Further, SLTIIv-producingstrains of E. cofi have only been isolated from pigs while the SLTIIva-producingE. coli strain exam ...
Classification of plant-pathogenic mycoplasma
... Based on Southern hybridization with a DNA fragment of an MLO associated with aster yellows, a differentiation between organisms inducing decline symptoms and those causing floral virescence has been proposed (Kuske et al., 1991). Although several organisms have been differentiated using these metho ...
... Based on Southern hybridization with a DNA fragment of an MLO associated with aster yellows, a differentiation between organisms inducing decline symptoms and those causing floral virescence has been proposed (Kuske et al., 1991). Although several organisms have been differentiated using these metho ...
LecturesPart07
... and X17254 (human) erythroid transcription factors using Entrez Do Pustell Protein Matrix Analysis ...
... and X17254 (human) erythroid transcription factors using Entrez Do Pustell Protein Matrix Analysis ...
PDF - Oxford Academic
... of the Lbc varieties. Available amino acid sequence data suggest that in this case the determined DNA sequence corresponds to Lbc^. However, this assignment is not conclusive since amino acid sequence analysis has not yet been completed on homogenous Lbc varieties (Whittaker, R.G., personal communic ...
... of the Lbc varieties. Available amino acid sequence data suggest that in this case the determined DNA sequence corresponds to Lbc^. However, this assignment is not conclusive since amino acid sequence analysis has not yet been completed on homogenous Lbc varieties (Whittaker, R.G., personal communic ...
Document
... • the two-hit method • gapped BLAST • PSI-BLAST all are aimed at increasing sensitivity while keeping run-times minimal Altschul, S.F., Madden, T.L., Schäffer, A.A., Zhang, J., Zhang, Z., Miller, W. & Lipman, D.J. (1997). Gapped BLAST and PSI-BLAST: a new generation of protein database search progra ...
... • the two-hit method • gapped BLAST • PSI-BLAST all are aimed at increasing sensitivity while keeping run-times minimal Altschul, S.F., Madden, T.L., Schäffer, A.A., Zhang, J., Zhang, Z., Miller, W. & Lipman, D.J. (1997). Gapped BLAST and PSI-BLAST: a new generation of protein database search progra ...
BioInformatics at FSU
... specify a particular biological molecular entity, be it polypeptide or nucleotide. The symbols are the one letter alphabetic codes for all of the biological nitrogenous bases and amino acid residues and their ambiguity codes. Biological carbohydrates, lipids, and structural information are not inclu ...
... specify a particular biological molecular entity, be it polypeptide or nucleotide. The symbols are the one letter alphabetic codes for all of the biological nitrogenous bases and amino acid residues and their ambiguity codes. Biological carbohydrates, lipids, and structural information are not inclu ...
Sequence and phylogenetic analysis of the gp200 protein of
... Ehrlichia (E.) canis is a Gram-negative obligate intracellular bacterium responsible for canine monocytic ehrlichiosis. Currently, the genetic diversity of E. canis strains worldwide is poorly defined. In the present study, sequence analysis of the nearly full-length 16S rDNA (1,620 bp) and the comp ...
... Ehrlichia (E.) canis is a Gram-negative obligate intracellular bacterium responsible for canine monocytic ehrlichiosis. Currently, the genetic diversity of E. canis strains worldwide is poorly defined. In the present study, sequence analysis of the nearly full-length 16S rDNA (1,620 bp) and the comp ...
Database Searching
... lineages. Each COG consists of individual proteins or groups of paralogs from at least 3 lineages and thus corresponds to an ancient conserved domain. Proteins from two eukaryotic genomes (Drosophila melanogaster and Caenorhabditis elegans) were assigned to COGs and can be reached from each individu ...
... lineages. Each COG consists of individual proteins or groups of paralogs from at least 3 lineages and thus corresponds to an ancient conserved domain. Proteins from two eukaryotic genomes (Drosophila melanogaster and Caenorhabditis elegans) were assigned to COGs and can be reached from each individu ...
Evolution of b-type cytochromes in prokaryotes
... cytochrome b561 (K12262) sequences (dataset as in Figure 4), was generated using Muscle, and visualized in Jalview. The four helices (pink) predicted by PSIpred for the E. coli sequence, along with the 6 helices based on the structure of the Arabidopsis protein, as reported in Figure S3 of (Lu et al ...
... cytochrome b561 (K12262) sequences (dataset as in Figure 4), was generated using Muscle, and visualized in Jalview. The four helices (pink) predicted by PSIpred for the E. coli sequence, along with the 6 helices based on the structure of the Arabidopsis protein, as reported in Figure S3 of (Lu et al ...
View PDF
... Citation: Perez-Marquez J (2014) SQRestriction: Bio-Informatics Software for Restriction Fragment Length Polymorphism of Batches of Sequences. J Comput Sci Syst Biol 7: 186-192. doi:10.4172/jcsb.1000155 Copyright: © 2014 Perez-Marquez J. This is an open-access article distributed under the terms of ...
... Citation: Perez-Marquez J (2014) SQRestriction: Bio-Informatics Software for Restriction Fragment Length Polymorphism of Batches of Sequences. J Comput Sci Syst Biol 7: 186-192. doi:10.4172/jcsb.1000155 Copyright: © 2014 Perez-Marquez J. This is an open-access article distributed under the terms of ...
Document
... 2. These 10 regions are rescored using Dayhoff PAM-250 residue exchange matrix (Dayhoff et al., 1983) and the best scoring region of the 10 is reported under init1 in the FASTA output. 3. Regions scoring higher than a threshold value and being sufficiently near each other in the sequence are joined, ...
... 2. These 10 regions are rescored using Dayhoff PAM-250 residue exchange matrix (Dayhoff et al., 1983) and the best scoring region of the 10 is reported under init1 in the FASTA output. 3. Regions scoring higher than a threshold value and being sufficiently near each other in the sequence are joined, ...
Molecular Cloning and Nucleotide Sequence of the 3
... clearly read from one end to the other. However, if there were two proximal CluI sites or an EcoRI or BglII site which could not be detected by mapping with restriction endonucleases, they would be missed. To test this possibility we cleaved pYKL45 with one of ClaI, EcoRI and BglII completely, recir ...
... clearly read from one end to the other. However, if there were two proximal CluI sites or an EcoRI or BglII site which could not be detected by mapping with restriction endonucleases, they would be missed. To test this possibility we cleaved pYKL45 with one of ClaI, EcoRI and BglII completely, recir ...
"An Introduction to Sequence Similarity ("Homology") Searching". In
... MAKING AND USING MULTIPLE SEQUENCE ALIGNMENTS The previous sections of this unit have focused on aligning pairs of sequences, or more generally searching an entire database for high-scoring alignments to a query sequence, which requires many pairwise comparisons. However, if one has several members ...
... MAKING AND USING MULTIPLE SEQUENCE ALIGNMENTS The previous sections of this unit have focused on aligning pairs of sequences, or more generally searching an entire database for high-scoring alignments to a query sequence, which requires many pairwise comparisons. However, if one has several members ...
Dehydrogenase in Saccharomyces cerevisiae
... at least 76 amino acids. A search of the NBRF protein data base showed no strong homology between the two predicted amino acid sequences and any known protein. Primary structure of yeast lipoamide dehydrogenase The primary structure, translated from the nucleotide sequence of the LPDl gene, contains ...
... at least 76 amino acids. A search of the NBRF protein data base showed no strong homology between the two predicted amino acid sequences and any known protein. Primary structure of yeast lipoamide dehydrogenase The primary structure, translated from the nucleotide sequence of the LPDl gene, contains ...
Dehydrogenase in Saccharomyces cerevisiae
... at least 76 amino acids. A search of the NBRF protein data base showed no strong homology between the two predicted amino acid sequences and any known protein. Primary structure of yeast lipoamide dehydrogenase The primary structure, translated from the nucleotide sequence of the LPDl gene, contains ...
... at least 76 amino acids. A search of the NBRF protein data base showed no strong homology between the two predicted amino acid sequences and any known protein. Primary structure of yeast lipoamide dehydrogenase The primary structure, translated from the nucleotide sequence of the LPDl gene, contains ...