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phylogenetic analysis of the rompb genes of rickettsia felis and
phylogenetic analysis of the rompb genes of rickettsia felis and

... on serologic data and subsequently isolates of R. prowazekii from flying squirrels in the eastern United States.5 Its transmission among flying squirrels by their own lice and fleas has also been described.5,6 Since then, reports of human cases of epidemic typhus in the eastern United States have ap ...
Applied and Environmental Microbiology
Applied and Environmental Microbiology

... were not amplified by PCR. Sequence analysis revealed that their corresponding primer targets contain numerous mismatches, especially in the inner parts of the primer regions. The PCR-amplified genes of the isolates were subjected to further characterization by restriction enzyme digestion (Table 1) ...
Bioinformatics in Drug Design
Bioinformatics in Drug Design

... bacteria) that inhabited the earth billions of years ago. It is also believed that eukaryotes evolved from Archaea, because they share many mRNA sequences, have similar RNA polymerases, and have introns. • Therefore, it is generally assumed that the domains Archaea and Bacteria branched from each ot ...
BMC Microbiology
BMC Microbiology

... Ola-51T and Enterobacter sp. CBMB30, which have been isolated from respectively the phyllosphere of wheat, the rhizosphere of groundnut and the endosphere of rice species (i.e. Oryza latifolia and O. sativa), can improve the fitness of their host plants and are therefore known as plant-growth-promot ...
Resistance to Antibiotics Mediated by Target Alterations
Resistance to Antibiotics Mediated by Target Alterations

... Penicillin and other members of the 6-1attam family of antibiotics kill bacteria by inactivating a set of transpeptidases that catalyze the final cross-linking reactions of peptidoglycan synthesis (9). Penicillin inhibits these enzymes by acting as a structural analog, forming an irreversible penici ...
dna sequence analysis: pairwise comparison
dna sequence analysis: pairwise comparison

... BestFit uses the local homology algorithm of Smith and Waterman (Advances in Applied Mathematics 2; 482-489 (1981)) to find the best segment of similarity between two sequences. BestFit reads a scoring matrix that contains values for every possible GCG symbol match. The program uses these values to ...
Characterization of Low Molecular Weight Glutenin Subunits by
Characterization of Low Molecular Weight Glutenin Subunits by

... void volume included all glutenins and some co-gliadins but excluded a-, /3-, and -y-gliadins. The glutenin and w-gliadin mixture was separated further by IE chromatography on carboxymethylcellulose (Whatman CM-32) using the method of Shewry et al (1981) with minor differences; mainly, 6M urea was i ...
PhyPA: Phylogenetic method with pairwise sequence alignment
PhyPA: Phylogenetic method with pairwise sequence alignment

... I have developed and implemented a phylogenetic reconstruction method based on PSA only (named PhyPA for Phylogenetics by Pairwise Alignment) for highly diverged sequences (One should use likelihood-based method whenever sequences are not highly diverged and reliable MSA can be obtained). I evaluate ...
Analysis of similarity of the S1 gene in infectious bronchitis virus (IBV
Analysis of similarity of the S1 gene in infectious bronchitis virus (IBV

... FCoV, CCoV and HCoV 229E) and group 2 (HCoV OC43, BCoV and MHoV) coronaviruses. In addition, higher identity could be observed between PSH and IBV strains compared with TCoV strains. The deduced amino acid sequence of S1 gene of the isolate PSH was most similar to that of field IBV isolates SH1 (87. ...
Document
Document

... as an input to the sequence analysis server. The sequence of this protein is determined before carrying out further studies for analyzing similarity matches with other proteins. ...
GST II: ---Title--- - Digital Biology Laboratory
GST II: ---Title--- - Digital Biology Laboratory

... The first substitution matrices derived by Dayhoff et al. (1978)  PAM (point accepted mutation) distance: Two sequences are defined to have diverged by one PAM unit if they show in average one accepted point mutation (i.e. one amino acid change) per hundred amino acids.  Derived from the pairwise ...
Gene encoding the group B streptococcal protein R4, its
Gene encoding the group B streptococcal protein R4, its

... characterized the prevalence of R4 in invasive disease, but information on the gene sequence of R4 is not known. ...
Discovery of Paralogous Nuclear Gene Sequences Coding for the
Discovery of Paralogous Nuclear Gene Sequences Coding for the

... amino acid changes may be too rare to reveal any information about relationships among closely related taxa, and some synonymous changes may be informative at certain levels of the tree. Agosti, Jacobs, and DeSalle (1996) suggested that nucleic acid sequences and their translated amino acid codings ...
Evidence for evolution of canine parvovirus type 2 in Italy
Evidence for evolution of canine parvovirus type 2 in Italy

... revealed by this panel of MAbs. The PCR typing approach was also unable to identify the variation that occurred in Glu-426 mutants. Type 2b-specific primers have been designed to take advantage of only two nucleotide variations, and the variation A G at nucleotide 4062, responsible for the change As ...
Cloning and Sequence Analysis of the xylL Gene Responsible for
Cloning and Sequence Analysis of the xylL Gene Responsible for

... pCSP21 digested with ClaI was introduced into the polyclonal region of pBluescript II SK(+) vector to make pRES3. The subclones of pRES301, pRES401, and pRES403 were further constructed by digestion of the pCSP21 with various enzymes. The physical maps of those clones are shown in Fig. 2. Editing an ...
The Nucleotide Sequence of a Type 3 Poliovirus Isolated During a
The Nucleotide Sequence of a Type 3 Poliovirus Isolated During a

... shown in Fig. 1. The genome comprises a 5' non-coding region of 746 nucleotides, a single open reading frame of 6618 nucleotides (2206 codons), a short 3' non-coding region of 71 nucleotides and a poly(A) tract. Sequence comparisons indicate that strain 23127 is highly homologous to the representati ...
The Nucleotide Sequence of a Type 3 Poliovirus Isolated During a
The Nucleotide Sequence of a Type 3 Poliovirus Isolated During a

... shown in Fig. 1. The genome comprises a 5' non-coding region of 746 nucleotides, a single open reading frame of 6618 nucleotides (2206 codons), a short 3' non-coding region of 71 nucleotides and a poly(A) tract. Sequence comparisons indicate that strain 23127 is highly homologous to the representati ...
- Wiley Online Library
- Wiley Online Library

... ~Received August 10, 1999; Final Revision October 20, 1999; Accepted October 22, 1999! ...
Bosque, a software system for phylogenetic analysis
Bosque, a software system for phylogenetic analysis

... Tree Projects and Tree Windows The current molecular phylogenetic analyses use nucleotides and amino-acids sequence data to infer the phylogeny of organisms. These analyses, therefore, begin with the integration of a set of sequences of interest and then by their respective alignment, which is the i ...
Gluconacetobacter entanii sp. nov., isolated from submerged high
Gluconacetobacter entanii sp. nov., isolated from submerged high

... 4560T, LTH 4341, LTH 4551 and LTH 4637) were characterized in more detail and it was revealed that they have in common certain properties such as requirement of acetic acid, ethanol and glucose for growth, and no overoxidation of acetate. Growth occurs only at total concentrations (sum of acetic aci ...
Dot Plot - APBioNET
Dot Plot - APBioNET

...  Homology: When two similar proteins come from a common ancestor. Homology is inferred from Similarity If two sequences are similar, then they are known as homologous sequences. Usually, at least 30% identity over 400 bp for DNA sequences and over 125 amino acids for proteins. ...
Colicins produced by the Escherichia fergusonii strains closely
Colicins produced by the Escherichia fergusonii strains closely

... EF3) was isolated after insertion of Tn7 into the plasmid backbone, resulting in the colicinogenic plasmid DS300. The colicin E1 cea gene encoded by this plasmid had a one-nucleotide replacement (G211A) when compared to cea of pColE1-EF43, resulting in a one-amino acid change (A71T) in colicin E1 pr ...
Dynamic Programming
Dynamic Programming

... The first four sequences possibly derive from closely related species and the last three from three more distant species. Since A occurs with high frequency in the first four sequences, the observed number of pairings of A with A will be higher than is appropriate if we are comparing more distantly ...
Nucleotide Sequence of the DNA Complementary to Avian (Chicken
Nucleotide Sequence of the DNA Complementary to Avian (Chicken

... It was not surprising to find that the greatest conservation in sequence homology was in the amino terminal 1-34 portion of the hormones, since this region has been shown to be responsible for the biological activity of PTH (2, 3). Within this region, the amino-terminal Ser-Val sequence is identical ...
Comparison of Sample Sequences of the Genome to the Sequence K-12
Comparison of Sample Sequences of the Genome to the Sequence K-12

... these differences often contribute to the differences in life strategies of related species (18, 31). If multiple loci are available from multiple related species, then it is also possible to identify some of the loci that appear to have a phylogeny different from that of the rest of the genome. The ...
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Multilocus sequence typing

Multilocus sequence typing (MLST) is a technique in molecular biology for the typing of multiple loci. The procedure characterizes isolates of microbial species using the DNA sequences of internal fragments of multiple housekeeping genes. Approximately 450-500 bp internal fragments of each gene are used, as these can be accurately sequenced on both strands using an automated DNA sequencer. For each housekeeping gene, the different sequences present within a bacterial species are assigned as distinct alleles and, for each isolate, the alleles at each of the loci define the allelic profile or sequence type (ST).The first MLST scheme to be developed was for Neisseria meningitidis, the causative agent of meningococcal meningitis and septicaemia. Since its introduction for the research of evolutionary history, MLST has been used not only for human pathogens but also for plant pathogens. To assist the gathering and formatting of the utilized sequences a simple and free plug-in for Firefox has been developed (link).
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