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PDF
PDF

... TT(AG)TT(TC)TG(AG)AACCAGAT(TC)TT(AGT)AT ...
pptx - Central Web Server 2
pptx - Central Web Server 2

... community. At the community level the outcome is a higher diversity, and a richer network of metabolic reactions. Presumably the more diverse communities are more stable towards perturbations, and provided the community can propagate as a whole, this would provide a selective advantage to the commun ...
Document
Document

... • Over long periods of time a sequence will acquire random mutations. – These mutations may result in a new amino acid at a given position, the deletion of an amino acid, or the introduction of a new one. – Over VERY long periods of time two sequences may diverge so much that their relationship can ...
Sequencing and Phylogeny - World Health Organization
Sequencing and Phylogeny - World Health Organization

... Sequencing Process to determine the exact order of nucleotides in DNA ...
Allele-Level Sequencing and Phasing of Full
Allele-Level Sequencing and Phasing of Full

... genetic analysis to determine histocompatibility. They are highly polymorphic and have thousands of alleles implicated in disease resistance and susceptibility. The importance of full-length HLA gene sequencing for genotyping, detection of null alleles, and phasing is now widely acknowledged. While ...
DNA Sequence Analysis
DNA Sequence Analysis

... Cycle Sequencing ...
Taxonomic distribution of Large DNA viruses in the sea
Taxonomic distribution of Large DNA viruses in the sea

... large viruses enclosed in the GOS metagenomic data.  The highest genetic richness corresponded to phages.  The Mimiviridae represent a major and ubiquitous ...
Specialties: Microbial Diversity and Bacterial Membrane Proteins
Specialties: Microbial Diversity and Bacterial Membrane Proteins

... as possible from the “high-risk” areas indicated by the survey study of IDEXX, (2) amplify the 16S rRNA genes and compare the RFPL (restriction fragment length polymorphism) patterns to study the genetic diversity of the collection, and (3) generate a dendrogram based upon the diversity of the RFLP ...
Challenge:
Challenge:

... to classify organisms. To gain a better understanding of what they are using, define the following: a. Homology b. Conserved sequence c. Phylogenic tree When we have DNA or protein sequences from many organisms, we can compare them to one another in order to determine which organisms are more closel ...
Identification and Classification of Prokaryote
Identification and Classification of Prokaryote

... Identify Prokaryotes  Nucleic acid probes can locate unique ...
Genetic_Research_Lesson9_Slides_Single_Sequence_NWABR
Genetic_Research_Lesson9_Slides_Single_Sequence_NWABR

... Circle #1: Example of a series of the same nucleotide (many T’s in a row). Notice the highest peaks are visible at each position. Circle #2: Example of an ambiguous base call. Notice the T (Red) at position 57 (highlighted in blue) is just below a green peak (A) at the same position. Look at the poo ...
Genetic Research Lesson 9 Single Sequence
Genetic Research Lesson 9 Single Sequence

... PowerPoint slides to accompany ...
Naming Conventions The NCBI RefSeq human mRNA
Naming Conventions The NCBI RefSeq human mRNA

... Naming Conventions The NCBI RefSeq human mRNA database represents the best effort in defining the most complete and authentic mRNA sequences encoded by the human genome. It currently contains approximately 17,500 "NM" sequences, which have at least some cDNA sequence support, and about 10,000 "XM" s ...
Characterization of the protein recognized by the monoclonal
Characterization of the protein recognized by the monoclonal

... In Europe, Borrelia burgdorferi sensu lato isolates belong to 4 major species: B. burgdorferi sensu stricto, B. afzelii, B. garinii and B. valaisiana. The objective of this study was to characterize low molecular weight proteins of B. burgdorferi sensu lato. Our main focus was a protein around 12 kD ...
PS401 – Lec 10
PS401 – Lec 10

... patterns and phenotypes  Molecular modeling database, conserved domain database, conserved domain architecture retrieval tool. ...
Lab
Lab

... • For proteins a different scoring matrix used, optimized for smaller evolutionary distances ...
Freshman Seminar
Freshman Seminar

... chain termination method – Also known as Sanger sequencing, after its ...
Summary of sixth lesson
Summary of sixth lesson

... – The Red Queen Hypothesis: selection pressure will increase number of resistant plant genotypes ...
Phylogenetics workshop 2
Phylogenetics workshop 2

... • Use DNA sequences that are evolving slowly – mutations happen rarely. • Use long stretches of DNA. • Align sequences, use the parts of the alignment that show a high degree of ...
Molecular Typing Of microorganisms
Molecular Typing Of microorganisms

... characterize a microbial isolate beyond species level and determine its sub-species, strain, or even sub-strain. To relate individual cases to an outbreak of infectious disease To establish an association between an outbreak of food poisoning and a specific food vehicle To study variations in the pa ...
Chapter 17: Microbial taxonomy
Chapter 17: Microbial taxonomy

...  Oligonucleotide signature sequences are short conserved sequences specific for a phylogenetically defined group of organisms  either complete or, more often, specific rRNA fragments can be compared  when comparing rRNA sequences between 2 organisms, their relatedness is represented by an associa ...
(Pulse-field Gel Electrophoresis)
(Pulse-field Gel Electrophoresis)

... PFGE for enteric bacteria – Salmonella, E. coli (STEC), Shigella, & Campylobacter, and coming soon….Listeria!! Pure enteric isolates from General Microbiology (human/veterinary) or Environmental Microbiology (food/environmental) are suspended in agar plugs (jello-like matrix) and treated to release ...
Note_on_isolation_and_DNA_extraction_of_rhizobia
Note_on_isolation_and_DNA_extraction_of_rhizobia

... “dominant marker” data that may be used to characterises the core-genome: for example using, “ERIC-PCR”. c. Diversity may also be assessed using sequence data gathered for key symbiotic genes such as “nodD-PCR” and “nodA-PCR”, and we have used these predominantly for typing isolates for Rhizobium le ...
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Multilocus sequence typing

Multilocus sequence typing (MLST) is a technique in molecular biology for the typing of multiple loci. The procedure characterizes isolates of microbial species using the DNA sequences of internal fragments of multiple housekeeping genes. Approximately 450-500 bp internal fragments of each gene are used, as these can be accurately sequenced on both strands using an automated DNA sequencer. For each housekeeping gene, the different sequences present within a bacterial species are assigned as distinct alleles and, for each isolate, the alleles at each of the loci define the allelic profile or sequence type (ST).The first MLST scheme to be developed was for Neisseria meningitidis, the causative agent of meningococcal meningitis and septicaemia. Since its introduction for the research of evolutionary history, MLST has been used not only for human pathogens but also for plant pathogens. To assist the gathering and formatting of the utilized sequences a simple and free plug-in for Firefox has been developed (link).
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