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The DNA sequence of the gene and genetic control sites for the
The DNA sequence of the gene and genetic control sites for the

... frequently follows the sequence ala-X-ala (13) although there is considerable variation in the cleavage site sequence. The five Bacillus signal sequences previously reported include four for which the cleavage site is known from comparisons between the sequence of the precursor protein inferred from ...
Discriminating E. coli Isolated from Various Human and Nonhuman
Discriminating E. coli Isolated from Various Human and Nonhuman

... Ten sewage isolates not in data base were grouped in HM cluster on MONOVA plot. Seven of the 10 sewage isolates were identified as human isolates based on maximum similarity. ...
Phylogenetic analysis
Phylogenetic analysis

... • 1) BLOSUM (Henikoff). These matrices appear to be the best available for carrying out data base similarity (homology searches). • 2) PAM (Dayhoff). These have been extremely widely used since the late '70s. • 3) GONNET. These matrices were derived using almost the same procedure as the Dayhoff one ...
Guidelines for ISBT Naming of Blood Group Alleles
Guidelines for ISBT Naming of Blood Group Alleles

... Alleles that encode some ISBT numbered antigens, but which do not differ from the Reference Allele, will not be given an allele name, e.g., LW6 (LWab). Alleles not relevant to transfusion medicine are not necessarily listed. Phenotypes and alleles may be listed in more than one place. Information wi ...
local alignments - LSU School of Medicine
local alignments - LSU School of Medicine

... (nr) protein sequence database is very large but sometimes confusing. ...
We have developed, for the IBM-PC
We have developed, for the IBM-PC

... page main menu is shown in Figure 1. The individual programs are described below, along with the amount of memory each occupies when manipulating 3,000 nucleotides, and their approximate execution times. Enter is used to type sequence data into the computer. It is designed to facilitate typing with ...
PowerPoint
PowerPoint

... First, we need a way to assign numbers to “shades of grey” matches. Genetic code scoring system – This assumes that changes in protein sequence arise from mutations. If only one point mutation is needed to change a given AA to another (at a specific position in alignment), the two amino-acids are mo ...
gyrA AND SEQUENCING METHOD
gyrA AND SEQUENCING METHOD

... contribution. In this study, all of the isolates resistant to nalidixic acid had a single mutation in gyrA. However, efflux pump activity could be less important in decreasing ciprofloxacin susceptibility levels, atleast the low level of resistance observed in these isolates. It seems likely that ov ...
Felis domesticus papillomavirus, isolated from a skin lesion, is
Felis domesticus papillomavirus, isolated from a skin lesion, is

... 1994), among others. The FdPV NCR-1 contains a variety of putative regulatory elements, although the FdPV NCR-1 does not include a TATA box within the E6\E7 promoter region. However, multiple SP-1 binding sites were identified that could serve as the transcription initiation site (Peterson et al., 1 ...
Identification of several different lineages of measles virus
Identification of several different lineages of measles virus

... number of divergent sequences. The data show that the MV strains analysed so far fall into a number of more or less closely related groups. Group A consists of the laboratory-adapted Edmonston vaccine strains, including the Moraten and Schwarz vaccine-associated cases and the Hall6 strain of SSPE vi ...
A phage library and two cosmid libraries were
A phage library and two cosmid libraries were

... Two cosmid libraries were prepared according to different experimental protocols (Materials and Methods). For the construction of both libraries (I and II) Mspl partial digests of the same human placenta DNA were used. The insert of the M13 subclone 1-1 was used to screen both cosmid libraries. A to ...
JCOOPIS-diagrams
JCOOPIS-diagrams

... Screen shot of the myGrid form with which a user can find services and construct simple workflows. 1) User selects values from a drop down list to create a property based description of their required service. Values are constrained to provide only sensible alternatives. 2) Once the user has entere ...
Roseobacter gallaeciensis sp. nov., a new marine - HAL
Roseobacter gallaeciensis sp. nov., a new marine - HAL

... representatives of the Proteobacteria (data not shown), indicated that BS107T was clustered within the a subclass of the Proteobacteria. The position of this bacterium within the a subclass was investigated. The three methods described in Methods were congruent and showed that the new bacterium clus ...
Analyses of complete nucleotide sequence of Iranian isolate of
Analyses of complete nucleotide sequence of Iranian isolate of

... Iran have been investigated by Zare et al. 2004. Similar studies have been made on nucleotide sequence of 5′-region of CP gene of the virus. Here, we analyze the complete genome sequence of MDMV-Ir to show its evolutionary and phylogenetic relationship with other isolates of the virus in the Mediter ...
Phylogenetic analysis of MADS
Phylogenetic analysis of MADS

... their homologs from 5 gymnosperms (specfically, DAL2, DAL11, DAL12, and DAL13 from Picea abies; PrDGL from Pinus radiata; SAG1a from Picea mariana; CyAG from Cycas edentate; and GBM5 from Ginkgo biloba). Based on the results, a phylogenetic tree was constructed using the neighbor-joining (NJ) method ...
Modification of the terminal restriction fragment length polymorphism
Modification of the terminal restriction fragment length polymorphism

... database with the same primers and subsequent calculation of theoretical AluI TRF sizes. The method of multiple colors T-RFLP profiles can be mostly used to identify the percentage of a specific taxonomic group in the total diversity of a particular soil microbial community. In fact, the same appro ...
Sequence Search
Sequence Search

... – In the next iteration, the new PSSM is used to score the alignments • A new PSSM is generated based on the significant alignments ...
pptx - Central Web Server 2
pptx - Central Web Server 2

... community. At the community level the outcome is a higher diversity, and a richer network of metabolic reactions. Presumably the more diverse communities are more stable towards perturbations, and provided the community can propagate as a whole, this would provide a selective advantage to the commun ...
PowerPoint Presentation - MCB 371/372
PowerPoint Presentation - MCB 371/372

... community. At the community level the outcome is a higher diversity, and a richer network of metabolic reactions. Presumably the more diverse communities are more stable towards perturbations, and provided the community can propagate as a whole, this would provide a selective advantage to the commun ...
No Slide Title
No Slide Title

... any better than sum of optimal pairwise alignments. 2. Create heuristic multiple alignment in ad hoc fashion to create a lower bound on MA score (using a guide tree). 3. Search N-dimensional scoring matrix for the best score including i-th element of sequence 1, j-th of sequence 2, k-th of sequence ...
microglobulin gene in the miiuy croaker, Miichthys miiuy
microglobulin gene in the miiuy croaker, Miichthys miiuy

... Major histocompatibility complex (MHC) class I molecules are located on the surface of vertebrate nucleated cells, and are involved in presenting foreign peptide products to cytotoxic CD8+ T cells by the degradation of intracellular pathogens (Srisapoome et al., 2004). They consist of one membrane-s ...
Multiple Sequence Alignments and Sequence Profiles
Multiple Sequence Alignments and Sequence Profiles

... Using motifs ...
BLAST
BLAST

... Compares a nucleotide query sequence translated in all reading frames against a protein sequence database. You could use this option to find potential translation products of an unknown nucleotide sequence. Compares a protein query sequence against a nucleotide sequence database dynamically translat ...
Phaeospirillum oryzae sp. nov., a spheroplast
Phaeospirillum oryzae sp. nov., a spheroplast

... Gram-negative, motile purple non-sulfur bacteria were isolated from rhizosphere soils of paddy and were characterized by a polyphasic taxonomic approach. Bacteriochlorophyll a and carotenoids, rhodopin, lycopene and rhodopin glucoside, were present as photosynthetic pigments. Intracellular photosynt ...
Glossary - ChristopherKing.name
Glossary - ChristopherKing.name

... homology. An E-value of 10-10 for a sequence can be interpreted as, “a 1 in 1010 chance that the sequence was pulled from the database by chance alone (has no homology to the query sequence).” ExPASy – Expert Protein Analysis System - us.expasy.org/ A server maintained by the Swiss Institute of Bioi ...
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Multilocus sequence typing

Multilocus sequence typing (MLST) is a technique in molecular biology for the typing of multiple loci. The procedure characterizes isolates of microbial species using the DNA sequences of internal fragments of multiple housekeeping genes. Approximately 450-500 bp internal fragments of each gene are used, as these can be accurately sequenced on both strands using an automated DNA sequencer. For each housekeeping gene, the different sequences present within a bacterial species are assigned as distinct alleles and, for each isolate, the alleles at each of the loci define the allelic profile or sequence type (ST).The first MLST scheme to be developed was for Neisseria meningitidis, the causative agent of meningococcal meningitis and septicaemia. Since its introduction for the research of evolutionary history, MLST has been used not only for human pathogens but also for plant pathogens. To assist the gathering and formatting of the utilized sequences a simple and free plug-in for Firefox has been developed (link).
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