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... The NCBI page described PSI blast as follows: ...
Protein Sequence Analysis Structure & Function Prediction
Protein Sequence Analysis Structure & Function Prediction

... – pattern recognition is used to detect similarity between sequences & hence to infer related structures & functions – ab initio prediction is used to deduce structure, & to infer ...
Course Outline
Course Outline

... BIOINFORMATICS COURSE OBJECTIVES To equip the students with the requisite background in areas of modern biology (biochemistry, cell biology, genetics and molecular biology) and computer science (programming languages, databases, algorithms, graphics, data mining, data security etc.). To expose the s ...
Sequencing genomes
Sequencing genomes

... A, Hide W. CLU: a new algorithm for EST clustering. BMC Bioinformatics. 2005; 6 Suppl 2:S3. PubMed PMID: 16026600 ...
FAN: fingerprint analysis of nucleotide sequences
FAN: fingerprint analysis of nucleotide sequences

... E-value of one for a database the size of PRINTS—not surprisingly, the fingerprint is unrelated. The sequence shown in the figure has a frameshift-causing sequence error; however, because it does not occur within a motif-matching region, it has had no effect on the significance of the match. The rel ...
Sequencing genomes
Sequencing genomes

... A new species of frog has been introduced into an area where it has too few natural predators. In an attempt to restore the ecological balance, a team of scientists is considering introducing a species of bird which feeds on this frog. Experimental data suggests that the population of frogs and bird ...
Biological sequence analysis
Biological sequence analysis

... translated portion: parts called introns (intervening sequence) are removed, leaving exons (expressed sequence), and yet other regions remain untranslated. The translated sequence comes in triples called codons, beginning and ending with a unique start (ATG) and one of three stop (TAA, TAG, TGA) cod ...
Protocol can be had here.
Protocol can be had here.

... will need to amplify the gene using PCR primers. Designing PCR primers is based on approximately 15 base pair complementary regions to the template DNA. Primer-BLAST2 is an online program that can allow such design. To use this program, you need to use a FASTA format for the sequence of interest. FA ...
Sequencing genomes
Sequencing genomes

... A new species of frog has been introduced into an area where it has too few natural predators. In an attempt to restore the ecological balance, a team of scientists is considering introducing a species of bird which feeds on this frog. Experimental data suggests that the population of frogs and bird ...
PowerPoint - IBIVU - Vrije Universiteit Amsterdam
PowerPoint - IBIVU - Vrije Universiteit Amsterdam

... transferred to X as a good suggestion. ...
BLAST- bioinformatics
BLAST- bioinformatics

... • Probabilities are calculated as being less than, or greater than observed by random chance, hence the negative numbers. ...
Genomics of sensory systems
Genomics of sensory systems

... Genbank has too many links Pull up many things that are not what you want  BLAST ...
Molecular Sequence Programs
Molecular Sequence Programs

... number of species and the number of sites. As with the other programs, options information may follow this. Following this, each species starts on a new line. The first 10 characters of that line are the species name. There then follows the base sequence of that species, each character being one of ...
Karakterisasi Molekular Fragmen Gen mexB Isolat Pseudomonas
Karakterisasi Molekular Fragmen Gen mexB Isolat Pseudomonas

... ABSTRACT Antibiotics have been widely used in the treatment of infectious diseases. However, their effectiveness has been questioned due to the tendency of some bacterial resistance to antibiotics. Pseudomonas aeruginosa among others has been known to be resistant to several antibiotics due to its M ...
Database Searching and Pairwise Alignment
Database Searching and Pairwise Alignment

... • Genetic code is degenerate - silent mutations ...
Genetic Transformation computer exercise v02 r01
Genetic Transformation computer exercise v02 r01

... mutated (GeneB) genes; this is known as a DNA sequence alignment. An alignment uses an algorithm (a step-by-step procedure) to compare the order of nucleotide bases in the sequences and then lines them up so that the number of identical bases is maximized. The alignment program will point out those ...
How to Raise the Dead: The Nuts and Bolts of Ancestral Sequence
How to Raise the Dead: The Nuts and Bolts of Ancestral Sequence

... Homology vs. Identity • Significant BLAST hits inform us about evolutionary relationships • Homologous - share a common ancestor – This is binary, not a percentile – Identity is calculated, homology is a hypothesis – Homology does not ensure common function ...
Introducing DOTUR, a Computer Program for Defining Operational
Introducing DOTUR, a Computer Program for Defining Operational

... • What is the expected number of OTUs in a microbial community? • How to determine the minimum number of sequences to estimate the overall OTUs? ...
Genetic Transformation computer exercise
Genetic Transformation computer exercise

... mutated (GeneB) genes; this is known as a DNA sequence alignment. An alignment uses an algorithm (a step-by-step procedure) to compare the order of nucleotide bases in the sequences and then lines them up so that the number of identical bases is maximized. The alignment program will point out those ...
Phylogenetic analysis
Phylogenetic analysis

... Modern phylogeny is based on genetic data and DNA sequence comparison Advance in DNA sequencing techniques made large-scale sequencing practical and more affordable allowing for a huge accumulation of sequence data for any organism of interest. Data sequences of highly conserved genes across all org ...
Practical 1
Practical 1

... If  you  haven't  already  downloaded  and  installed  R  in  your  computer  you  should  do  it  now.  The   easiest  way  is  to  go  to  http://www.rstudio.com/  and  install  the  latest  version  of  RStudio.   R  is  a  langu ...
Amino acid sequence alignment of a `small` citrate synthase from
Amino acid sequence alignment of a `small` citrate synthase from

... cerevisiae is well documented [3], evidence has now been provided suggesting the presence of two CS genes in Escherichia coli [4] and Bacillus subtilis [S], organisms considered for many years to contain a single molecular form of CS. There is also evidence that CS isoenzymes may have different meta ...
A Tutorial of Sequence Matching and Alignment in Oracle
A Tutorial of Sequence Matching and Alignment in Oracle

... BLASTN_MATCH -- nucleotide sequence BLASTP_MATCH – amino acid sequences TBLAST_MATCH -- sequence involving ...
introducing single molecule real-time (smrt
introducing single molecule real-time (smrt

... HLA typing plays a major role in the selection of haematopoietic stem cell donors and there is published evidence that higher resolution HLA matching is associated with increased overall survival. Presently, many high-throughput molecular HLA typing approaches like PCR-SSOP or SBT lead to ambiguous ...
Identification and Classification of Prokaryote
Identification and Classification of Prokaryote

... Identify Prokaryotes  Nucleic acid probes can locate unique ...
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Multilocus sequence typing

Multilocus sequence typing (MLST) is a technique in molecular biology for the typing of multiple loci. The procedure characterizes isolates of microbial species using the DNA sequences of internal fragments of multiple housekeeping genes. Approximately 450-500 bp internal fragments of each gene are used, as these can be accurately sequenced on both strands using an automated DNA sequencer. For each housekeeping gene, the different sequences present within a bacterial species are assigned as distinct alleles and, for each isolate, the alleles at each of the loci define the allelic profile or sequence type (ST).The first MLST scheme to be developed was for Neisseria meningitidis, the causative agent of meningococcal meningitis and septicaemia. Since its introduction for the research of evolutionary history, MLST has been used not only for human pathogens but also for plant pathogens. To assist the gathering and formatting of the utilized sequences a simple and free plug-in for Firefox has been developed (link).
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