Gonococcal outer-membrane protein PIB
... those from other serovars reveals that sequence variations occur in two discrete regions of the molecule centred on residues 1% (Varl) and 237 (Var2). A series of peptides spanning the amino acid sequence of the protein were synthesized on solid-phase supports and reacted with a panel of monoclonal ...
... those from other serovars reveals that sequence variations occur in two discrete regions of the molecule centred on residues 1% (Varl) and 237 (Var2). A series of peptides spanning the amino acid sequence of the protein were synthesized on solid-phase supports and reacted with a panel of monoclonal ...
Protein sequence analysis
... The filter option, if set to true, will allow you to mask out various segments of the query sequence for regions which are nonspecific for sequence similarity searches. Filtering can eliminate statistically significant but biologically uninteresting reports from the output, for example hits against ...
... The filter option, if set to true, will allow you to mask out various segments of the query sequence for regions which are nonspecific for sequence similarity searches. Filtering can eliminate statistically significant but biologically uninteresting reports from the output, for example hits against ...
Molecular characterization of MHC class II in a nonmodel anuran
... histocompatibility complex (MHC), the organization of this gene complex has never been studied in other anurans, and information on amphibian MHC (other than Xenopus) is generally very scarce. Here, we describe the characterization of the first MHC class II B cDNA sequences from a nonmodel anuran sp ...
... histocompatibility complex (MHC), the organization of this gene complex has never been studied in other anurans, and information on amphibian MHC (other than Xenopus) is generally very scarce. Here, we describe the characterization of the first MHC class II B cDNA sequences from a nonmodel anuran sp ...
Identification of sixteen single-nucleotide polymorphism markers in
... was extracted from adductor muscle using the E.Z.N.A Mollusc DNA Kit (Omega Bio-Tek, Norcross, USA). Primers were first examined for PCR in eight wild individuals. PCR was performed in 20 µL reaction volume containing 12.3 µL H2 O miliQ, 2 µL PCR buffer (Mg2+ Plus) 10×, 1.6 µL dNTP 2.5 mM, 0.8 µL of ...
... was extracted from adductor muscle using the E.Z.N.A Mollusc DNA Kit (Omega Bio-Tek, Norcross, USA). Primers were first examined for PCR in eight wild individuals. PCR was performed in 20 µL reaction volume containing 12.3 µL H2 O miliQ, 2 µL PCR buffer (Mg2+ Plus) 10×, 1.6 µL dNTP 2.5 mM, 0.8 µL of ...
Document
... A popular way to identify similarities between proteins is to perform a pairwise alignment (Blast, Fasta). When the identity is higher than 40% this method gives good results. ...
... A popular way to identify similarities between proteins is to perform a pairwise alignment (Blast, Fasta). When the identity is higher than 40% this method gives good results. ...
... For measuring the similarity between sequences two approaches generally are used; global alignment and local alignment. In global similarity algorithms, the entire lengths of sequences are subject for representing the best alignment. This method will produce an alignment with large stretches of low ...
Analyses of 16S rRNA and RuBisCO large subunit genes from an
... cbbL.2, X70355; Thiobacillus denitrificans, L42940; Synechococcus sp. PCC6301, X03220; Rhodospirillum rubrum, X00286; Methanococcus jannaschii, U67564. The residue identities in all alignment sequences are marked with asterisks, conserved substitutions are marked with colons, and semiconserved subst ...
... cbbL.2, X70355; Thiobacillus denitrificans, L42940; Synechococcus sp. PCC6301, X03220; Rhodospirillum rubrum, X00286; Methanococcus jannaschii, U67564. The residue identities in all alignment sequences are marked with asterisks, conserved substitutions are marked with colons, and semiconserved subst ...
2 Identifying Conserved Elements in the Toxin Sensor and
... Genome sequences are stored in the gene databank (GenBank) of the National Institute of Health (NIH). GenBank is the annotated collection of publicly available gene sequences. By the end of 2013 there was 169,331,407 sequences stored in the GenBank, which represents a 17-fold increase in data since ...
... Genome sequences are stored in the gene databank (GenBank) of the National Institute of Health (NIH). GenBank is the annotated collection of publicly available gene sequences. By the end of 2013 there was 169,331,407 sequences stored in the GenBank, which represents a 17-fold increase in data since ...
DNA Sequencing
... (Sanger) Sequencing • With addition of enzyme (DNA polymerase), the primer is extended until a ddNTP is encountered. • The chain will end with the incorporation of the ...
... (Sanger) Sequencing • With addition of enzyme (DNA polymerase), the primer is extended until a ddNTP is encountered. • The chain will end with the incorporation of the ...
Complementary DNA Sequence of a Human Cytoplasmic Actin
... muscle tissue. The other two, /3- and y-a&in, are called cytoplasmic actins and are typical of non-muscle tissue (Vandekerckhove & Weber, 19786). In the human genome. there are more than 20 actin genes as estimated by hybridization studies using cloned complementary DNA probes derived from mouse or ...
... muscle tissue. The other two, /3- and y-a&in, are called cytoplasmic actins and are typical of non-muscle tissue (Vandekerckhove & Weber, 19786). In the human genome. there are more than 20 actin genes as estimated by hybridization studies using cloned complementary DNA probes derived from mouse or ...
Populus trichocarpa
... – Direct positive selection – are some of these alleles conditionally advantageous? ...
... – Direct positive selection – are some of these alleles conditionally advantageous? ...
Multiple Alignment
... protein family (motifs) MSA can help us to reveal biological facts about proteins, like analysis of the secondary/tertiary structure) ...
... protein family (motifs) MSA can help us to reveal biological facts about proteins, like analysis of the secondary/tertiary structure) ...
IOSR Journal of Pharmacy and Biological Sciences (IOSRJPBS)
... physiological characteristics. Molecular techniques involving ribosomal RNA sequence analysis are commonly used to investigate evolutionary relationships within different genera of bacteria. In a routine antibiotic screening strategy, the strains producing interesting metabolites in screening are us ...
... physiological characteristics. Molecular techniques involving ribosomal RNA sequence analysis are commonly used to investigate evolutionary relationships within different genera of bacteria. In a routine antibiotic screening strategy, the strains producing interesting metabolites in screening are us ...
The Art of Multiple Sequence Alignment in R - decipher
... of the same kind. In this case, multiple alignment works by aligning two sequences, merging with another sequence, merging with another set of sequences, and so-forth until all the sequences are aligned. This process is iterated to further refine the alignment. There are other functions that extend ...
... of the same kind. In this case, multiple alignment works by aligning two sequences, merging with another sequence, merging with another set of sequences, and so-forth until all the sequences are aligned. This process is iterated to further refine the alignment. There are other functions that extend ...
Identification, cloning and sequence determination of genes specifying hexokinase A and B from yeast.
... site was translated to give -Thr-Gly-His-Ile-Ala-Ala-, where the carboxy terminal Ala overlapped the amino terminal Ala from the first subclone. Since this tryptic peptide had been tentatively assigned as spanning residues 385-396 in the approximated structure derived from x-ray crystallography (5), ...
... site was translated to give -Thr-Gly-His-Ile-Ala-Ala-, where the carboxy terminal Ala overlapped the amino terminal Ala from the first subclone. Since this tryptic peptide had been tentatively assigned as spanning residues 385-396 in the approximated structure derived from x-ray crystallography (5), ...
The Amino Acid Sequences of Cytochrome c from Four Plant Sources
... been of great value in studying the phylogenetic relationships among living organisms (Dayhoff, 1972). The protein studied most extensively in this connexion has been mitochondrial cytochrome c. A computer-generated phylogenetic tree for a range of higher plant species has been established (Boulter ...
... been of great value in studying the phylogenetic relationships among living organisms (Dayhoff, 1972). The protein studied most extensively in this connexion has been mitochondrial cytochrome c. A computer-generated phylogenetic tree for a range of higher plant species has been established (Boulter ...
PPT
... If a merging repeat has references that belong to multiple distinct classes, then those classes are combined into one. If a merging repeat contains no reference to an existing class, then the merging repeat forms a new class. ...
... If a merging repeat has references that belong to multiple distinct classes, then those classes are combined into one. If a merging repeat contains no reference to an existing class, then the merging repeat forms a new class. ...
Clustering Method for Repeat Analysis in DNA sequences
... If a merging repeat has references that belong to multiple distinct classes, then those classes are combined into one. If a merging repeat contains no reference to an existing class, then the merging repeat forms a new class. ...
... If a merging repeat has references that belong to multiple distinct classes, then those classes are combined into one. If a merging repeat contains no reference to an existing class, then the merging repeat forms a new class. ...
Phylogeny of Firmicutes with special reference to Mycoplasma
... From comparison of different substitution models and different tree phylogeny algorithms, molecular phylogenetic analyses of Pgk sequences resulted in identical tree topologies with high bootstrap support values (Fig. 1). The monophyletic Mollicutes (bootstrap support of up to 95 %) are the sister g ...
... From comparison of different substitution models and different tree phylogeny algorithms, molecular phylogenetic analyses of Pgk sequences resulted in identical tree topologies with high bootstrap support values (Fig. 1). The monophyletic Mollicutes (bootstrap support of up to 95 %) are the sister g ...
Lack of homology between two haloacetate dehalogenase genes
... dehH2 gene product corresponded closely to those of the H-2 enzyme (Table 1). The dehH2 gene was preceded by the sequence GGAGA, the putative Shine-Dalgarno sequence, present 6 bp upstream from the ATG initiation codon. A promoter that initiates transcription of dehH2 in E. coli must be present in t ...
... dehH2 gene product corresponded closely to those of the H-2 enzyme (Table 1). The dehH2 gene was preceded by the sequence GGAGA, the putative Shine-Dalgarno sequence, present 6 bp upstream from the ATG initiation codon. A promoter that initiates transcription of dehH2 in E. coli must be present in t ...
That have been aligned so that homologous residues are arranged
... Progressive alignment is fast and simple but it does have one obvious drawback: a local minimum problem. Any alignment errors that occur during the early alignment steps cannot be corrected later as more data are added. This may be due to an incorrect topology in the guide tree, but it is more like ...
... Progressive alignment is fast and simple but it does have one obvious drawback: a local minimum problem. Any alignment errors that occur during the early alignment steps cannot be corrected later as more data are added. This may be due to an incorrect topology in the guide tree, but it is more like ...
Bioinformatics Toolbox
... scientists who need to develop new algorithms or implement published ones, visualize results, and create stand-alone applications. ...
... scientists who need to develop new algorithms or implement published ones, visualize results, and create stand-alone applications. ...
Table 6. Ascus type distribution of Table 4. Linkage of colony morphology
... structural locus (nit1) or the pathway-specific regulatory locus (nit3). Population studies may require pairings of scores of VCG standards with hundreds of field isolates, making VCG testing an arduous task. We have reduced the effort and space required for these pairings by using disposable, plast ...
... structural locus (nit1) or the pathway-specific regulatory locus (nit3). Population studies may require pairings of scores of VCG standards with hundreds of field isolates, making VCG testing an arduous task. We have reduced the effort and space required for these pairings by using disposable, plast ...
Histone genes of Volvox carteri: DNA sequence and organization of
... genes (9), differences in gene structure between replication type (H3.1 and H3.2) and replacement-type (H3.3) gene families are paralleled by specific differences in amino acid sequences, codon usage and mode of expression. Based on these data a heterodox evolutionary model in which the intron-beari ...
... genes (9), differences in gene structure between replication type (H3.1 and H3.2) and replacement-type (H3.3) gene families are paralleled by specific differences in amino acid sequences, codon usage and mode of expression. Based on these data a heterodox evolutionary model in which the intron-beari ...