Immunology 4
... about an effective immune response against the particular pathogen by interfering with its reproduction or killing it, etc. Two different kinds of epitopes exist, discontinuous epitopes occur due to amino acids or different points in a given antigen molecule being brought together from some distance ...
... about an effective immune response against the particular pathogen by interfering with its reproduction or killing it, etc. Two different kinds of epitopes exist, discontinuous epitopes occur due to amino acids or different points in a given antigen molecule being brought together from some distance ...
Immunological Memory
... 5. How long is the latent period for this infection? 6. Explain this delay in production of antibodies. 7. The person was infected with the same antigen at 4 weeks. Use an arrow to mark on the graph the time of the second infection. 8. The person does not suffer any symptoms from the second infectio ...
... 5. How long is the latent period for this infection? 6. Explain this delay in production of antibodies. 7. The person was infected with the same antigen at 4 weeks. Use an arrow to mark on the graph the time of the second infection. 8. The person does not suffer any symptoms from the second infectio ...
Architect Annoucement
... The ARCHITECT HIV Ag/Ab Combo assay is a chemiluminescent microparticle immunoassay (CMIA) for the simultaneous qualitative detection of human immunodeficiency virus (HIV) p24 antigen and antibodies to HIV type 1 (HIV-1 group M and group O) and/or type 2 (HIV-2) in human serum and plasma (EDTA and h ...
... The ARCHITECT HIV Ag/Ab Combo assay is a chemiluminescent microparticle immunoassay (CMIA) for the simultaneous qualitative detection of human immunodeficiency virus (HIV) p24 antigen and antibodies to HIV type 1 (HIV-1 group M and group O) and/or type 2 (HIV-2) in human serum and plasma (EDTA and h ...
MCB 181 (Nov 4 – Dec 4) Information and Heredity
... animals such as rabbits, sheep, goats, donkeys – Antibodies recovered from blood at a certain time-point ...
... animals such as rabbits, sheep, goats, donkeys – Antibodies recovered from blood at a certain time-point ...
LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
... BI 3951 - IMMUNOTECHNOLOGY Date & Time : 30-10-2006/9.00-12.00 ...
... BI 3951 - IMMUNOTECHNOLOGY Date & Time : 30-10-2006/9.00-12.00 ...
LOYOLA COLLEGE (AUTONOMOUS), CHENNAI
... (a) B cell (b) Macrophage (c) Mast cell (d) Dendritic cell (2) Which one of the following has antiviral activity? (a) Interferons (b) Interleukins (c) Cytokines (d) Chemokines (3) Tissue typing can be done using (a) Immunodiffusion (b) Mixed lymphocyte reaction (c) Agglutination (d) Elispot assay (4 ...
... (a) B cell (b) Macrophage (c) Mast cell (d) Dendritic cell (2) Which one of the following has antiviral activity? (a) Interferons (b) Interleukins (c) Cytokines (d) Chemokines (3) Tissue typing can be done using (a) Immunodiffusion (b) Mixed lymphocyte reaction (c) Agglutination (d) Elispot assay (4 ...
Monoclonal Antibodies
... (cancer) cells + antigen immunized mouse spleen cells. Monoclonal antibodies can be used for diagnostic tests, treatment and research. ...
... (cancer) cells + antigen immunized mouse spleen cells. Monoclonal antibodies can be used for diagnostic tests, treatment and research. ...
Slide 1
... Purpose Describe antibodies (ab) and antigens (ag) and how they work. Purify IgY antibodies from egg yolk and identify using the dot-blot method of immunodetection. Describe immune response and relate to vaccination. Explain how principles of immunology are used in clinical diagnostics. ...
... Purpose Describe antibodies (ab) and antigens (ag) and how they work. Purify IgY antibodies from egg yolk and identify using the dot-blot method of immunodetection. Describe immune response and relate to vaccination. Explain how principles of immunology are used in clinical diagnostics. ...
TIRP Antibody
... TIRP is a member of the Toll/interleukin-1 receptor (TIR) family, a group of proteins that include the Toll-like receptors (TLRs) (1-3). TLRs are signaling molecules that recognize different pathogen-associated molecular patterns (PAMPs) and serve as an important link between the innate and adaptive ...
... TIRP is a member of the Toll/interleukin-1 receptor (TIR) family, a group of proteins that include the Toll-like receptors (TLRs) (1-3). TLRs are signaling molecules that recognize different pathogen-associated molecular patterns (PAMPs) and serve as an important link between the innate and adaptive ...
Immune System
... Indirect contact - through the air or by touching objects that have been infected and than touching your nose or mouth. Contaminated Food and Water – caused by eating food containing pathogens or drinking un-sanitized water. Infected Animals - Vectors carry diseases like Lyme disease, West Nil ...
... Indirect contact - through the air or by touching objects that have been infected and than touching your nose or mouth. Contaminated Food and Water – caused by eating food containing pathogens or drinking un-sanitized water. Infected Animals - Vectors carry diseases like Lyme disease, West Nil ...
12.2 Review Questions What happens when serum containing B
... 4. Briefly describe how antibodies capable of reacting with drugs are produced in animals. 5. What immunoassay technique has gained widespread popularity among toxicologists because of its speed and high sensitivity for detecting drugs in urine? What drug is this technique frequently used to screen ...
... 4. Briefly describe how antibodies capable of reacting with drugs are produced in animals. 5. What immunoassay technique has gained widespread popularity among toxicologists because of its speed and high sensitivity for detecting drugs in urine? What drug is this technique frequently used to screen ...
Chapter 10 Classification
... • Based on cell shape, gram reaction, biochemical testing • Enzyme activities • Urea --- urease--- ammonium ions ...
... • Based on cell shape, gram reaction, biochemical testing • Enzyme activities • Urea --- urease--- ammonium ions ...
Immune System Performances
... 1. In your group, read your specific case study. 2. Create a diagram or map that shows the interaction between the invading antigen and the immune system. The diagrams of different groups may be similar, but there are variations in the type antigen causing the reaction, mode of entry, transmission, ...
... 1. In your group, read your specific case study. 2. Create a diagram or map that shows the interaction between the invading antigen and the immune system. The diagrams of different groups may be similar, but there are variations in the type antigen causing the reaction, mode of entry, transmission, ...
LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
... 5. Hybridoma technology was first developed by a) Kohler b) Mittelman c) Yallow ...
... 5. Hybridoma technology was first developed by a) Kohler b) Mittelman c) Yallow ...
SG9 Immune Response
... Differentiate between innate and acquired immunity. Differentiate between immunity and nonspecific resistance. Contrast the four types of acquired immunity. Define antigen. Explain the function of antibodies and describe their structural and chemical characteristics. Name the function of B cells. De ...
... Differentiate between innate and acquired immunity. Differentiate between immunity and nonspecific resistance. Contrast the four types of acquired immunity. Define antigen. Explain the function of antibodies and describe their structural and chemical characteristics. Name the function of B cells. De ...
Powerpoint 5
... An ELISA test uses components of the immune system and chemicals to detect immune responses in the body (for example, to infectious microbes). The ELISA test involves an enzyme (a protein that catalyzes a biochemical reaction). It also involves an antibody or antigen (immunologic molecules). What is ...
... An ELISA test uses components of the immune system and chemicals to detect immune responses in the body (for example, to infectious microbes). The ELISA test involves an enzyme (a protein that catalyzes a biochemical reaction). It also involves an antibody or antigen (immunologic molecules). What is ...
TBH1 - Cellabs
... following values to the seven standard curve samples and comparing the optical densities of the test samples with the control samples. Standard No ...
... following values to the seven standard curve samples and comparing the optical densities of the test samples with the control samples. Standard No ...
Weatherall Institute of Molecular Medicine (WIMM) University of
... Cloning, expression, and purification of FcγR-His (I-IV) Immobilize FcγR-His onto a NiNTA plate with antibodies and using ELISA to test for IL-2 resulting from T-cell activation Immobilize FcγR-His onto a supported lipid bilayer with antibodies Calcium assays to test for T-cell activation ...
... Cloning, expression, and purification of FcγR-His (I-IV) Immobilize FcγR-His onto a NiNTA plate with antibodies and using ELISA to test for IL-2 resulting from T-cell activation Immobilize FcγR-His onto a supported lipid bilayer with antibodies Calcium assays to test for T-cell activation ...
LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
... d) nucleic acid II. State whether the following are True or False; state reason 6. CD 45 is a signal transduction molecule found on B lymphocytes. 7. Dendritic cell is not an example of professional antigen presenting cell. 8. Apoptosis is a physiological phenomena rather than a pathological one. 9. ...
... d) nucleic acid II. State whether the following are True or False; state reason 6. CD 45 is a signal transduction molecule found on B lymphocytes. 7. Dendritic cell is not an example of professional antigen presenting cell. 8. Apoptosis is a physiological phenomena rather than a pathological one. 9. ...
ELISA
The enzyme-linked immunosorbent assay (ELISA) (/ɨˈlaɪzə/, /ˌiːˈlaɪzə/) is a test that uses antibodies and color change to identify a substance.ELISA is a popular format of ""wet-lab"" type analytic biochemistry assay that uses a solid-phase enzyme immunoassay (EIA) to detect the presence of a substance, usually an antigen, in a liquid sample or wet sample.The ELISA has been used as a diagnostic tool in medicine and plant pathology, as well as a quality-control check in various industries.Antigens from the sample are attached to a surface. Then, a further specific antibody is applied over the surface so it can bind to the antigen. This antibody is linked to an enzyme, and, in the final step, a substance containing the enzyme's substrate is added. The subsequent reaction produces a detectable signal, most commonly a color change in the substrate.Performing an ELISA involves at least one antibody with specificity for a particular antigen. The sample with an unknown amount of antigen is immobilized on a solid support (usually a polystyrene microtiter plate) either non-specifically (via adsorption to the surface) or specifically (via capture by another antibody specific to the same antigen, in a ""sandwich"" ELISA). After the antigen is immobilized, the detection antibody is added, forming a complex with the antigen. The detection antibody can be covalently linked to an enzyme, or can itself be detected by a secondary antibody that is linked to an enzyme through bioconjugation. Between each step, the plate is typically washed with a mild detergent solution to remove any proteins or antibodies that are non-specifically bound. After the final wash step, the plate is developed by adding an enzymatic substrate to produce a visible signal, which indicates the quantity of antigen in the sample.Of note, ELISA can perform other forms of ligand binding assays instead of strictly ""immuno"" assays, though the name carried the original ""immuno"" because of the common use and history of development of this method. The technique essentially requires any ligating reagent that can be immobilized on the solid phase along with a detection reagent that will bind specifically and use an enzyme to generate a signal that can be properly quantified. In between the washes, only the ligand and its specific binding counterparts remain specifically bound or ""immunosorbed"" by antigen-antibody interactions to the solid phase, while the nonspecific or unbound components are washed away. Unlike other spectrophotometric wet lab assay formats where the same reaction well (e.g. a cuvette) can be reused after washing, the ELISA plates have the reaction products immunosorbed on the solid phase which is part of the plate, and so are not easily reusable.