Genome Control - University of California, Los Angeles
... • Engineered E. Coli cells only synthesizes carboyxl terminal of β galactosidase protein • pUC8 plasmid contains gene for amino terminal • If pUC8 transforms cells, gene is fully functional ...
... • Engineered E. Coli cells only synthesizes carboyxl terminal of β galactosidase protein • pUC8 plasmid contains gene for amino terminal • If pUC8 transforms cells, gene is fully functional ...
Bacteriophages
... Vectors based on Bacteriophage λ The λ genome is 48.5 kb, in which 15 kb or so is ‘optional’ it contains genes that are only needed for integration into the E. coli chromosome (controlling lysogenic properties) These segments can therefore be deleted without impairing the ability of the phage to in ...
... Vectors based on Bacteriophage λ The λ genome is 48.5 kb, in which 15 kb or so is ‘optional’ it contains genes that are only needed for integration into the E. coli chromosome (controlling lysogenic properties) These segments can therefore be deleted without impairing the ability of the phage to in ...
Biotechnology Glow Genes
... DNA Fingerprinting Unique system of bands that represent segments of DNA that were cut with restriction enzymes Look for matching bands to identify individuals or ...
... DNA Fingerprinting Unique system of bands that represent segments of DNA that were cut with restriction enzymes Look for matching bands to identify individuals or ...
F - cells
... Nucleoid region -Contains the single, circular chromosome -May also contain plasmids Ribosomes -Smaller than those of eukaryotes and differ in protein and RNA content -Targeted by antibacterial antibiotics ...
... Nucleoid region -Contains the single, circular chromosome -May also contain plasmids Ribosomes -Smaller than those of eukaryotes and differ in protein and RNA content -Targeted by antibacterial antibiotics ...
Calcium Phosphate Transfection Method
... The day before transfection, split fairly confluent (70-90%) culture between 1:10 and 1:15 (the ratio that we use depends on the cell lines being transfected and we typically split faster growing cell lines at the higher ratios – very slow growing cell lines may be split even less than 1:10). The ne ...
... The day before transfection, split fairly confluent (70-90%) culture between 1:10 and 1:15 (the ratio that we use depends on the cell lines being transfected and we typically split faster growing cell lines at the higher ratios – very slow growing cell lines may be split even less than 1:10). The ne ...
Genetic Transformation
... Cells are kept on ice until heat shock treatment Heat shock at 42 ˚C for one minute Recover period in LB broth Cells are spread on appropriate selection plates ...
... Cells are kept on ice until heat shock treatment Heat shock at 42 ˚C for one minute Recover period in LB broth Cells are spread on appropriate selection plates ...
Genetic Technology
... How do we make changes to DNA of an organism??? USING….Restriction Enzymes: bacterial enzymes act like scissors that cut DNA molecules at specific sites create sticky ends that can easily bind to complementary DNA and form new sequences of nucleotides ...
... How do we make changes to DNA of an organism??? USING….Restriction Enzymes: bacterial enzymes act like scissors that cut DNA molecules at specific sites create sticky ends that can easily bind to complementary DNA and form new sequences of nucleotides ...
2005-2006 AP Biology Biotech Tools Review 2005
... You want to produce human insulin cheaply for diabetics How do you find which bacteria carry the recombinant plasmid? amp resistance gene on plasmid LacZ gene plasmid ...
... You want to produce human insulin cheaply for diabetics How do you find which bacteria carry the recombinant plasmid? amp resistance gene on plasmid LacZ gene plasmid ...
![Recombinant DNA technology.ppt [Compatibility Mode]](http://s1.studyres.com/store/data/022508436_1-26bb714d45e9a2e7cd265480e0da1a03-300x300.png)
The Genetics of Bacteria and Their Viruses
... be transferred and about 2 minutes for the transfer of F • The difference reflects the relative sizes of F and the chromosome (100 kb versus 4600 kb) • Regions in the transferred DNA may incorporate into the recipient chromosome and replace homologous regions • This results in recombinant F- cells c ...
... be transferred and about 2 minutes for the transfer of F • The difference reflects the relative sizes of F and the chromosome (100 kb versus 4600 kb) • Regions in the transferred DNA may incorporate into the recipient chromosome and replace homologous regions • This results in recombinant F- cells c ...
Linkage
... • Plasmids are small, circular DNA molecules – Plasmids are found in the cytoplasm of many bacteria – Plasmids are not essential for survival of the cell – They may exist singly or in many copies – Plasmids have a variety of functions • Examples: metabolic, resistance, cryptic • Fertility plasmids, ...
... • Plasmids are small, circular DNA molecules – Plasmids are found in the cytoplasm of many bacteria – Plasmids are not essential for survival of the cell – They may exist singly or in many copies – Plasmids have a variety of functions • Examples: metabolic, resistance, cryptic • Fertility plasmids, ...
File
... Cloning serves two main purposes. 1- It allows a large number of recombinant DNA molecules to be produced from a limited amount of starting material In this way cloning can supply the large amounts of DNA needed for molecular biological studies of gene structure and expression ...
... Cloning serves two main purposes. 1- It allows a large number of recombinant DNA molecules to be produced from a limited amount of starting material In this way cloning can supply the large amounts of DNA needed for molecular biological studies of gene structure and expression ...
Supplemental Methods and Figure Legends
... Supplemental methods. Plasmids for expressing P. angusta H3 and H4 in S. cerevisiae: The S. cerevisiae HHT2 and HHF2 genes (respectively, chr. XIV coordinates 575,265-576,092 and 576,046-577,238) were amplified by PCR and cloned separately into pGEM-T (Promega). An XhoI site was incorporated into th ...
... Supplemental methods. Plasmids for expressing P. angusta H3 and H4 in S. cerevisiae: The S. cerevisiae HHT2 and HHF2 genes (respectively, chr. XIV coordinates 575,265-576,092 and 576,046-577,238) were amplified by PCR and cloned separately into pGEM-T (Promega). An XhoI site was incorporated into th ...
Ch. 12 Introduction to Biotechnology
... of the crime scenes (above), Narborough, England (left) ...
... of the crime scenes (above), Narborough, England (left) ...
Hfr cells
... How is the bacterial chromosome different from the eukaryotic chromosome? What other molecule contains useful genetic information for prokaryotes? Compare and contrast DNA replication in eukaryotes vs. prokaryotes. Why does the replication of every DNA molecule start with a short segment of RNA? Def ...
... How is the bacterial chromosome different from the eukaryotic chromosome? What other molecule contains useful genetic information for prokaryotes? Compare and contrast DNA replication in eukaryotes vs. prokaryotes. Why does the replication of every DNA molecule start with a short segment of RNA? Def ...
Mixed Questions
... What is this process called? 2. All mRNA produced in bacteria is polycistronic. True or false and explain. 3. List the types of macrolesions. 4. What is an episome? 5. Define the terms permissive and restrictive conditions. 6. Frameshift mutations generally have little effect on the protein product. ...
... What is this process called? 2. All mRNA produced in bacteria is polycistronic. True or false and explain. 3. List the types of macrolesions. 4. What is an episome? 5. Define the terms permissive and restrictive conditions. 6. Frameshift mutations generally have little effect on the protein product. ...
Concept 18.3. How get genetic variation in prokaryotes: • E. coli is
... - A prophage picks up a few adjacent genes as it leaves and transfers to a new host. - Transfer only of adjacent genes. ...
... - A prophage picks up a few adjacent genes as it leaves and transfers to a new host. - Transfer only of adjacent genes. ...
View PDF
... - A prophage picks up a few adjacent genes as it leaves and transfers to a new host. - Transfer only of adjacent genes. ...
... - A prophage picks up a few adjacent genes as it leaves and transfers to a new host. - Transfer only of adjacent genes. ...
Chapter 20 Notes: DNA Technology
... 5) Insert recombinant DNA plasmid back into bacterial cell; 6) As bacterial cell reproduces, it makes copies of the desired gene; -grow cells on a petri dish ...
... 5) Insert recombinant DNA plasmid back into bacterial cell; 6) As bacterial cell reproduces, it makes copies of the desired gene; -grow cells on a petri dish ...
presentation source
... can be similarly digested by restriction enzymes, and can be used as vectors • DNA strands can be spliced into the plasmid, and the plasmid re-inserted into a bacterium • As the bacteria divides, the spliced DNA becomes part of the genome and is also replicated • Certain viruses can also be used as ...
... can be similarly digested by restriction enzymes, and can be used as vectors • DNA strands can be spliced into the plasmid, and the plasmid re-inserted into a bacterium • As the bacteria divides, the spliced DNA becomes part of the genome and is also replicated • Certain viruses can also be used as ...
Restriction Enzyme Sequence
... The major tools of recombinant DNA technology are bacterial enzymes called restriction enzymes. Each enzyme recognizes a short, specific nucleotide sequence in DNA molecules, and cuts the backbones of the molecules at that sequence. The result is a set of double-stranded DNA fragments with single-st ...
... The major tools of recombinant DNA technology are bacterial enzymes called restriction enzymes. Each enzyme recognizes a short, specific nucleotide sequence in DNA molecules, and cuts the backbones of the molecules at that sequence. The result is a set of double-stranded DNA fragments with single-st ...
Plasmid
.svg?width=300)
A plasmid is a small DNA molecule within a cell that is physically separated from a chromosomal DNA and can replicate independently. They are most commonly found in bacteria as small, circular, double-stranded DNA molecules; however, plasmids are sometimes present in archaea and eukaryotic organisms. In nature, plasmids often carry genes that may benefit the survival of the organism, for example antibiotic resistance. While the chromosomes are big and contain all the essential information for living, plasmids usually are very small and contain only additional information. Artificial plasmids are widely used as vectors in molecular cloning, serving to drive the replication of recombinant DNA sequences within host organisms.Plasmids are considered replicons, a unit of DNA capable of replicating autonomously within a suitable host. However, plasmids, like viruses, are not generally classified as life. Plasmids can be transmitted from one bacterium to another (even of another species) via three main mechanisms: transformation, transduction, and conjugation. This host-to-host transfer of genetic material is called horizontal gene transfer, and plasmids can be considered part of the mobilome. Unlike viruses (which encase their genetic material in a protective protein coat called a capsid), plasmids are ""naked"" DNA and do not encode genes necessary to encase the genetic material for transfer to a new host. However, some classes of plasmids encode the conjugative ""sex"" pilus necessary for their own transfer. The size of the plasmid varies from 1 to over 200 kbp, and the number of identical plasmids in a single cell can range anywhere from one to thousands under some circumstances.The relationship between microbes and plasmid DNA is neither parasitic nor mutualistic, because each implies the presence of an independent species living in a detrimental or commensal state with the host organism. Rather, plasmids provide a mechanism for horizontal gene transfer within a population of microbes and typically provide a selective advantage under a given environmental state. Plasmids may carry genes that provide resistance to naturally occurring antibiotics in a competitive environmental niche, or the proteins produced may act as toxins under similar circumstances, or allow the organism to utilize particular organic compounds that would be advantageous when nutrients are scarce.