Supplementary Information
... by Geneart AG with the restriction sites NcoI and NotI at the 5’ and 3’ ends respectively in the vector pMA. The D-AAO was excised from the vector using NcoI and NotI and inserted into pRSF-Duet using the same restriction sites, to create pRSF-D-AAO. The AlaR gene was cloned into pET16b as described ...
... by Geneart AG with the restriction sites NcoI and NotI at the 5’ and 3’ ends respectively in the vector pMA. The D-AAO was excised from the vector using NcoI and NotI and inserted into pRSF-Duet using the same restriction sites, to create pRSF-D-AAO. The AlaR gene was cloned into pET16b as described ...
Submitted by Bryan Smith LaLumiere School LaPorte, IN A
... Except for the lab items list in materials section, each kit comes with all the necessary supplies. If you have micropipettes, these can be used in place of the ones that come in the Edvotek and Carolina kits. The Peyer kit is different in that company leases a thermocycler for PCR, a long wave UV l ...
... Except for the lab items list in materials section, each kit comes with all the necessary supplies. If you have micropipettes, these can be used in place of the ones that come in the Edvotek and Carolina kits. The Peyer kit is different in that company leases a thermocycler for PCR, a long wave UV l ...
Genetic Engineering
... one particular gene out of the tens of thousands of genes in an individual's genome. ...
... one particular gene out of the tens of thousands of genes in an individual's genome. ...
03 Biotechnology Note
... least 2 different sources – scientists use this DNA to mess with (manipulate) genes and proteins ...
... least 2 different sources – scientists use this DNA to mess with (manipulate) genes and proteins ...
Document
... • Technique used to maintain a stock of similar organisms Individuals with similar characteristics are crossed so the traits will appear again in next generation PROBLEM: Increased risk of recessive genetic defects. Example(s): Purebred dogs ...
... • Technique used to maintain a stock of similar organisms Individuals with similar characteristics are crossed so the traits will appear again in next generation PROBLEM: Increased risk of recessive genetic defects. Example(s): Purebred dogs ...
recombinant dna technology
... • FIRST, THE PLASMID IS TREATED WITH THE SAME RESTRICTION ENZYME AS WAS USED TO CREATE THE DNA FRAGMENT • THE RESTRICTION ENZYME WILL CUT THE PLASMID AT THE SAME RECOGNITION SEQUENCES, PRODUCING THE SAME STICKY ENDS CARRIED BY THE FRAGMENTS • MIXING THE FRAGMENTS WITH THE CUT PLASMIDS ALLOWS BASE-PA ...
... • FIRST, THE PLASMID IS TREATED WITH THE SAME RESTRICTION ENZYME AS WAS USED TO CREATE THE DNA FRAGMENT • THE RESTRICTION ENZYME WILL CUT THE PLASMID AT THE SAME RECOGNITION SEQUENCES, PRODUCING THE SAME STICKY ENDS CARRIED BY THE FRAGMENTS • MIXING THE FRAGMENTS WITH THE CUT PLASMIDS ALLOWS BASE-PA ...
Slide 1
... Recombinant DNA Vectors • autonomously-replicating DNA used to ‘carry’ and amplify foreign DNA within host cell • eg: plasmids, phage/viruses, or combinations Plasmids ...
... Recombinant DNA Vectors • autonomously-replicating DNA used to ‘carry’ and amplify foreign DNA within host cell • eg: plasmids, phage/viruses, or combinations Plasmids ...
Aim
... autofluorescence can be a defence mechanism to frighten away enemies or a lightning system in darkness. Yet autoflourescence is not needed for fierce animals, e.g. lions and tigers. A species preserves its integrity by mechanisms to actively destroy any non-self invaders. Bacteria biosynthesize rest ...
... autofluorescence can be a defence mechanism to frighten away enemies or a lightning system in darkness. Yet autoflourescence is not needed for fierce animals, e.g. lions and tigers. A species preserves its integrity by mechanisms to actively destroy any non-self invaders. Bacteria biosynthesize rest ...
Bacterial Transformation and Transfection Bacterial transformation is
... Bacterial cells containing plasmids with the antibiotic resistance gene are selected in bacterial transformations, and cells in an area of M13 infection are recognized as plaques against a lawn of non-infected cells. However, the object of most transformations and transfections is to clone foreign D ...
... Bacterial cells containing plasmids with the antibiotic resistance gene are selected in bacterial transformations, and cells in an area of M13 infection are recognized as plaques against a lawn of non-infected cells. However, the object of most transformations and transfections is to clone foreign D ...
File
... General principles of cell signaling, Extracellular signal molecule and their receptors, Operation of signaling molecules over various distances, Sharing of signal information, Cellular response to specific combinations of extracellular signal molecules; Different response by different cells to same ...
... General principles of cell signaling, Extracellular signal molecule and their receptors, Operation of signaling molecules over various distances, Sharing of signal information, Cellular response to specific combinations of extracellular signal molecules; Different response by different cells to same ...
What traits are carried on mobile
... the ecological factors involved in the persistence of plasmids are directed to a recent paper on the subject by Slater et al., 2008). Infectious elements such as plasmids are likely to face a tradeoff between horizontal transfer and vertical transmission, mediated by the costs that they impose on th ...
... the ecological factors involved in the persistence of plasmids are directed to a recent paper on the subject by Slater et al., 2008). Infectious elements such as plasmids are likely to face a tradeoff between horizontal transfer and vertical transmission, mediated by the costs that they impose on th ...
Lecture #9 Date
... Restriction Enzymes- used to isolate a specific gene Cloning Vectors- a carrier that is used to clone a gene and transfer it from one organism to another. Example: Plasmid ...
... Restriction Enzymes- used to isolate a specific gene Cloning Vectors- a carrier that is used to clone a gene and transfer it from one organism to another. Example: Plasmid ...
Plasmid Purification, Restriction Digest, and Lithium Acetate
... extremely important to be gentle at this step, as the cells are very delicate after the exposure to harsh chemicals and heat!). 18. Pellet the cells by spinning in a microfuge for one minute at 13,000rpm. Be sure to balance the microfuge, perhaps by coordinating with another group. 19. Remove approx ...
... extremely important to be gentle at this step, as the cells are very delicate after the exposure to harsh chemicals and heat!). 18. Pellet the cells by spinning in a microfuge for one minute at 13,000rpm. Be sure to balance the microfuge, perhaps by coordinating with another group. 19. Remove approx ...
Gene Technology – Revision Pack (B6)
... STEP 1 – scientists add genes that make the bacteria resistant to antibiotics STEP 2 – the bacteria are then grown on an agar containing the antibiotic STEP 3 – scientists ONLY choose the bacteria that survive (that are resistant to the antibiotic) because they have taken up the antibiotic plasmid, ...
... STEP 1 – scientists add genes that make the bacteria resistant to antibiotics STEP 2 – the bacteria are then grown on an agar containing the antibiotic STEP 3 – scientists ONLY choose the bacteria that survive (that are resistant to the antibiotic) because they have taken up the antibiotic plasmid, ...
clicker review
... 20. A scientist recovers a bit of tissue from the preserved skin of a 400 year old extinct Dodo bird and would like to compare it to samples from living birds. Which of the following would be most useful for increasing the amount of DNA for testing? A RFLP analysis B Polymerase chain reaction (PCR) ...
... 20. A scientist recovers a bit of tissue from the preserved skin of a 400 year old extinct Dodo bird and would like to compare it to samples from living birds. Which of the following would be most useful for increasing the amount of DNA for testing? A RFLP analysis B Polymerase chain reaction (PCR) ...
Recombinant DNA Technology (b)
... experimental system and how the cloned gene will be screened or utilized subsequently. Commonly used vectors are Plasmid, bacteriophage, cosmid, bacterial artificial chromosome (BAC), yeast artificial chromosome (YAC), yeast 2 micron plasmid, retrovirus, baculovirus vector ...
... experimental system and how the cloned gene will be screened or utilized subsequently. Commonly used vectors are Plasmid, bacteriophage, cosmid, bacterial artificial chromosome (BAC), yeast artificial chromosome (YAC), yeast 2 micron plasmid, retrovirus, baculovirus vector ...
Genetic engineering in budding yeast
... Because the flanks can be as little as 45bp, they can be added as part of a primer in a PCR reaction, so to create the above cassette, PCR amplify the ‘New sequence’ region with the flanks attached to the primers (this makes long oligos of ~65bp, but this does not effect the PCR). The un-purified PC ...
... Because the flanks can be as little as 45bp, they can be added as part of a primer in a PCR reaction, so to create the above cassette, PCR amplify the ‘New sequence’ region with the flanks attached to the primers (this makes long oligos of ~65bp, but this does not effect the PCR). The un-purified PC ...
Biocatalysis - Chatham University
... ESSENTIALS STEPS IN DNA CLONING 1. Cutting target DNA at precise locations. Sequence-specific endonucleases (restriction endonucleases) provide the necessary molecular scissors 2. Selecting a small carrier molecule of DNA capable of selfreplication. These DNAs are called cloning vectors (typically ...
... ESSENTIALS STEPS IN DNA CLONING 1. Cutting target DNA at precise locations. Sequence-specific endonucleases (restriction endonucleases) provide the necessary molecular scissors 2. Selecting a small carrier molecule of DNA capable of selfreplication. These DNAs are called cloning vectors (typically ...
Recombinant DNA Technology
... We can also tweak the plasmids to ensure expression of the new gene Cloning genes in eukaryotes Yeast artificial chromosomes (YACs) contain a yeast origin of replication, a pair of telomeres, and a centromere o These can carry inserted DNA fragments of 600-1000 kb But how do we find that one gen ...
... We can also tweak the plasmids to ensure expression of the new gene Cloning genes in eukaryotes Yeast artificial chromosomes (YACs) contain a yeast origin of replication, a pair of telomeres, and a centromere o These can carry inserted DNA fragments of 600-1000 kb But how do we find that one gen ...
E. coli
... 1970s, were natural plasmids originally from E. coli. • Structure and features: Plasmids are small in size, from 2 to around 200 kb extrachromosomal circular molecules which exist in multiple copies (up to a few hundreds) within the host E. coli cells. They contain an origin of replication ...
... 1970s, were natural plasmids originally from E. coli. • Structure and features: Plasmids are small in size, from 2 to around 200 kb extrachromosomal circular molecules which exist in multiple copies (up to a few hundreds) within the host E. coli cells. They contain an origin of replication ...
lecture 3 notes
... B. Type of reproductive behavior-eukaryotic microbes may be self-fertile, self-sterile (outcrossing), both or only asexual. Reproduction mostly occurs by conjugation. Transfer of nuclear material or whole cell fusion. ...
... B. Type of reproductive behavior-eukaryotic microbes may be self-fertile, self-sterile (outcrossing), both or only asexual. Reproduction mostly occurs by conjugation. Transfer of nuclear material or whole cell fusion. ...
Genetic Engineering
... Genes for herbicide resistance are inserted into crop plants. If the particular herbicide is then sprayed on to a crop, any weeds present will die; the crop plants will not be affected. There is concern that this resistance gene may be able to spread from the crop plants to closely related species o ...
... Genes for herbicide resistance are inserted into crop plants. If the particular herbicide is then sprayed on to a crop, any weeds present will die; the crop plants will not be affected. There is concern that this resistance gene may be able to spread from the crop plants to closely related species o ...
Plasmid
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A plasmid is a small DNA molecule within a cell that is physically separated from a chromosomal DNA and can replicate independently. They are most commonly found in bacteria as small, circular, double-stranded DNA molecules; however, plasmids are sometimes present in archaea and eukaryotic organisms. In nature, plasmids often carry genes that may benefit the survival of the organism, for example antibiotic resistance. While the chromosomes are big and contain all the essential information for living, plasmids usually are very small and contain only additional information. Artificial plasmids are widely used as vectors in molecular cloning, serving to drive the replication of recombinant DNA sequences within host organisms.Plasmids are considered replicons, a unit of DNA capable of replicating autonomously within a suitable host. However, plasmids, like viruses, are not generally classified as life. Plasmids can be transmitted from one bacterium to another (even of another species) via three main mechanisms: transformation, transduction, and conjugation. This host-to-host transfer of genetic material is called horizontal gene transfer, and plasmids can be considered part of the mobilome. Unlike viruses (which encase their genetic material in a protective protein coat called a capsid), plasmids are ""naked"" DNA and do not encode genes necessary to encase the genetic material for transfer to a new host. However, some classes of plasmids encode the conjugative ""sex"" pilus necessary for their own transfer. The size of the plasmid varies from 1 to over 200 kbp, and the number of identical plasmids in a single cell can range anywhere from one to thousands under some circumstances.The relationship between microbes and plasmid DNA is neither parasitic nor mutualistic, because each implies the presence of an independent species living in a detrimental or commensal state with the host organism. Rather, plasmids provide a mechanism for horizontal gene transfer within a population of microbes and typically provide a selective advantage under a given environmental state. Plasmids may carry genes that provide resistance to naturally occurring antibiotics in a competitive environmental niche, or the proteins produced may act as toxins under similar circumstances, or allow the organism to utilize particular organic compounds that would be advantageous when nutrients are scarce.