Curriculum Vitae
... 16. 20123131; PubMed Central PMCID: PMC2892030.Zhang H, Wang P, Papangelopoulos N, Xu Y, Sette A, Bourne PE, Lund O, Ponomarenko J, Nielsen M, Peters B. Limitations of Ab initio predictions of peptide binding to MHC class II molecules. PLoS One. 2010 Feb 17;5(2):e9272. PMCID: PMC2822856 17. Moutaft ...
... 16. 20123131; PubMed Central PMCID: PMC2892030.Zhang H, Wang P, Papangelopoulos N, Xu Y, Sette A, Bourne PE, Lund O, Ponomarenko J, Nielsen M, Peters B. Limitations of Ab initio predictions of peptide binding to MHC class II molecules. PLoS One. 2010 Feb 17;5(2):e9272. PMCID: PMC2822856 17. Moutaft ...
Rhesus macaque and mouse models for down
... human malaria vaccine formulations, but the rhesus model is expensive and does not allow for direct efficacy testing of human malaria vaccines. Transgenic rodent parasites expressing genes of human Plasmodium are now routinely used for efficacy studies of human malaria vaccines. Mice have however ra ...
... human malaria vaccine formulations, but the rhesus model is expensive and does not allow for direct efficacy testing of human malaria vaccines. Transgenic rodent parasites expressing genes of human Plasmodium are now routinely used for efficacy studies of human malaria vaccines. Mice have however ra ...
Generation of Monoclonal Antibodies to Cryptic Collagen Sites by
... (MMP) family of enzymes. To date little is known concerning the biochemical consequences of collagen metabolism on human diseases. This is due in part to the lack of specific reagents that can distinguish between proteolyzed and triple helical forms of collagen. Here we used the technique of Subtrac ...
... (MMP) family of enzymes. To date little is known concerning the biochemical consequences of collagen metabolism on human diseases. This is due in part to the lack of specific reagents that can distinguish between proteolyzed and triple helical forms of collagen. Here we used the technique of Subtrac ...
Licentiate thesis from the Department of Immunology,
... macrophages, and NK cells (Coban et al., 2005, Parroche et al., 2007). The mannose, scavenger and dectin receptors mediate binding and phagocytosis of microorganisms and foreign particles. Fc, complement and scavenger receptors further facilitate the clearance of microorganisms that are coated with ...
... macrophages, and NK cells (Coban et al., 2005, Parroche et al., 2007). The mannose, scavenger and dectin receptors mediate binding and phagocytosis of microorganisms and foreign particles. Fc, complement and scavenger receptors further facilitate the clearance of microorganisms that are coated with ...
Early cytokine release in response to live largely complement independent
... . These authors contributed equally to this work. ...
... . These authors contributed equally to this work. ...
Full-Text PDF
... structure motif and is unaffected by small sequence gaps between these units [24]. Therefore, as most CA125 tandem repeats are polymorphic, the SEA domain may not necessarily be 131 amino acids long. This is exemplified by the identification of a SEA domain consensus sequence with a length of 59 ami ...
... structure motif and is unaffected by small sequence gaps between these units [24]. Therefore, as most CA125 tandem repeats are polymorphic, the SEA domain may not necessarily be 131 amino acids long. This is exemplified by the identification of a SEA domain consensus sequence with a length of 59 ami ...
(CD32) Phenotype and Level of Expression
... of FcyRII (CD32)Phenotype and Level of Expression By Yoshiaki Tomiyama, Thomas J. Kunicki, Theodore F. Zipf, Sheila 6.Ford, and Richard H. Aster Certain monoclonal antibodies (MoAbs) specific for platelet membrane glycoproteins are known t o be capable of activating platelets, and it is generally th ...
... of FcyRII (CD32)Phenotype and Level of Expression By Yoshiaki Tomiyama, Thomas J. Kunicki, Theodore F. Zipf, Sheila 6.Ford, and Richard H. Aster Certain monoclonal antibodies (MoAbs) specific for platelet membrane glycoproteins are known t o be capable of activating platelets, and it is generally th ...
bacteriophages
... production, and most of the serological work with phage has been done with rabbit sera. The phages themselves are nontoxic and nonpathogenic for animals and can usually be injected in large amounts without damage to the recipient. Crude phage lysates, however, may contain toxic bacterial substances, ...
... production, and most of the serological work with phage has been done with rabbit sera. The phages themselves are nontoxic and nonpathogenic for animals and can usually be injected in large amounts without damage to the recipient. Crude phage lysates, however, may contain toxic bacterial substances, ...
Carbohydrate profiling reveals a distinctive role for
... and function within the immune system. It has been postulated that C-type lectins function in cell–cell adhesion, antigen recognition and serve as signaling molecules influencing the balance between tolerance and immunity (6). C-type lectin stimulation can either enhance or inhibit TLR signaling, th ...
... and function within the immune system. It has been postulated that C-type lectins function in cell–cell adhesion, antigen recognition and serve as signaling molecules influencing the balance between tolerance and immunity (6). C-type lectin stimulation can either enhance or inhibit TLR signaling, th ...
Common measures of immune function vary with time of day and
... therefore, it is possible that the variation in immune function with handling time reflects influences other than handling stress. For example, average time until blood sample collection increased with the number of birds captured at a given time. Therefore, a relationship between gregariousness and ...
... therefore, it is possible that the variation in immune function with handling time reflects influences other than handling stress. For example, average time until blood sample collection increased with the number of birds captured at a given time. Therefore, a relationship between gregariousness and ...
EndoS and SpeB from Streptococcus pyogenes
... using the glutathione S-transferase (GST) gene fusion system (Amersham-Pharmacia Biotech, Uppsala, Sweden) as described previously (5). A 2,929-bp PCR product including bases 304 to 3232 of the ndoS sequence was amplified from S. pyogenes genomic DNA by using primer 5⬘-ACT-GGG-ATC-CCG-GAGGAG-AAG-ACT ...
... using the glutathione S-transferase (GST) gene fusion system (Amersham-Pharmacia Biotech, Uppsala, Sweden) as described previously (5). A 2,929-bp PCR product including bases 304 to 3232 of the ndoS sequence was amplified from S. pyogenes genomic DNA by using primer 5⬘-ACT-GGG-ATC-CCG-GAGGAG-AAG-ACT ...
Transmission-Blocking Immunity against Malaria: From Antigen
... vector; secondly, restricted antigenic diversity is necessary for TBV antigens; furthermore, adjuvants should be capable to induce the output of excessive antibody titers after just one injection from the vaccinated individual as no natural boosting will take place. Molecules from the parasite or mo ...
... vector; secondly, restricted antigenic diversity is necessary for TBV antigens; furthermore, adjuvants should be capable to induce the output of excessive antibody titers after just one injection from the vaccinated individual as no natural boosting will take place. Molecules from the parasite or mo ...
ImmunoPursuit - Manchester Immunology Group
... intestines by diarrhoea that is symptomatic of the disease ...
... intestines by diarrhoea that is symptomatic of the disease ...
Monoclonal Antibodies Specific for Oxidative Phosphorylation
... Molecular Probes offers a range of subunit-specific antibodies for the study of mitochondrial function and dysfunction (Tables 13 and Figure 1), including monoclonal antibodies specific for subunits of human COX (Complex IV) and for representative subunits of human Complexes I, II, III and V. These ...
... Molecular Probes offers a range of subunit-specific antibodies for the study of mitochondrial function and dysfunction (Tables 13 and Figure 1), including monoclonal antibodies specific for subunits of human COX (Complex IV) and for representative subunits of human Complexes I, II, III and V. These ...
Development of an Antigen-independent Affinity Assay to Study the
... [4] (not indicated in Figure 1). FcγRs are expressed on all immune competent cells and most cells co-express activating and inhibiting receptors. It is generally accepted that NK cells, platelets and B-cells are exceptions to this as the two former only express activating receptors while the latter ...
... [4] (not indicated in Figure 1). FcγRs are expressed on all immune competent cells and most cells co-express activating and inhibiting receptors. It is generally accepted that NK cells, platelets and B-cells are exceptions to this as the two former only express activating receptors while the latter ...
Definition of the disease
... position (Seshadri et al., 2003). In general, the genomes of C. burnetii isolates from a wide range of biologically and geographically diverse sources are highly conserved, but notable polymorphism occurs such as rearrangement of syntenic blocks (Beare et al., 2009). This genomic plasticity might co ...
... position (Seshadri et al., 2003). In general, the genomes of C. burnetii isolates from a wide range of biologically and geographically diverse sources are highly conserved, but notable polymorphism occurs such as rearrangement of syntenic blocks (Beare et al., 2009). This genomic plasticity might co ...
Identification of Antigen-Specific B Cell Receptor Sequences Using
... Output files were converted into FASTA files, and sequences were assigned a sample and isotype based on the MID sequence. For two individuals at both time points, isotype-specific amplicons were sequenced without separate MID tags and, therefore, sequences were assigned an isotype by C region sequen ...
... Output files were converted into FASTA files, and sequences were assigned a sample and isotype based on the MID sequence. For two individuals at both time points, isotype-specific amplicons were sequenced without separate MID tags and, therefore, sequences were assigned an isotype by C region sequen ...
ACUTE ANTERIOR UVEITIS AND HLA-B27: INFECTIOUS BACKGROUND, SYSTEMIC INFLAMMATION,
... (TNF)-α production in response to bacterial lipopolysaccharide (LPS) was studied using whole blood culture assay. The levels of TNF-α in culture supernatants and soluble interleukin-2 receptor (sIL-2R) in serum were determined by chemiluminescent immunoassay (Immulite). The monocyte surface express ...
... (TNF)-α production in response to bacterial lipopolysaccharide (LPS) was studied using whole blood culture assay. The levels of TNF-α in culture supernatants and soluble interleukin-2 receptor (sIL-2R) in serum were determined by chemiluminescent immunoassay (Immulite). The monocyte surface express ...
Program - Canadian Society of Transplantation
... Subclinical tacrolimus nephrotoxicity in protocol biopsies obtained more than 5 years after renal allograft transplantation Trana Hussaini Investigating the Association between Hypomagnesaemia and Calcineurin Inhibitor Induced Nephrotoxicity in Liver Transplant Recipients: A Case-Control Study Yasem ...
... Subclinical tacrolimus nephrotoxicity in protocol biopsies obtained more than 5 years after renal allograft transplantation Trana Hussaini Investigating the Association between Hypomagnesaemia and Calcineurin Inhibitor Induced Nephrotoxicity in Liver Transplant Recipients: A Case-Control Study Yasem ...
OglIb~3 Integrin Dissociation Induced by EDTA Results in
... causes tension in others, resulting in their collapsed appearance. On the other hand, when human platelets are incubated for 30 min at 37"C with 5 mM EDTA and then resuspended in a calcium containing medium, they lose their ability to bind fibrinogen and to aggregate in response to ADP stimulation ( ...
... causes tension in others, resulting in their collapsed appearance. On the other hand, when human platelets are incubated for 30 min at 37"C with 5 mM EDTA and then resuspended in a calcium containing medium, they lose their ability to bind fibrinogen and to aggregate in response to ADP stimulation ( ...
Human ProBNP and proBNP-derived Peptides (BNP and NT
... ProBNP studies in human blood revealed that endogenous proBNP, as well as NT-proBNP, is glycosylated in the region 28-56 and is poorly recognized by antibodies specific to this region. However, in contrast to NT-proBNP, proBNP is also glycosylated in the region 61-76. Therefore, in the development o ...
... ProBNP studies in human blood revealed that endogenous proBNP, as well as NT-proBNP, is glycosylated in the region 28-56 and is poorly recognized by antibodies specific to this region. However, in contrast to NT-proBNP, proBNP is also glycosylated in the region 61-76. Therefore, in the development o ...
The Role of Donor‐Specific HLA Alloantibodies in Liver
... potential DSA-associated pathologies that all require further study, preferably as part of prospective clinical trials (Table 1). The relative contribution of antibodies to graft injury has been difficult to tease out because: (1) rejection, currently categorized as ‘‘cellular,’’ may represent a mix ...
... potential DSA-associated pathologies that all require further study, preferably as part of prospective clinical trials (Table 1). The relative contribution of antibodies to graft injury has been difficult to tease out because: (1) rejection, currently categorized as ‘‘cellular,’’ may represent a mix ...
Chromatin as a target antigen in human and murine lupus nephritis
... proposes that only those antibodies which cross-reacted with inherent renal antigens induced the organ disease. A nephritogenic potential of antibodies against DNA (or nucleosomes) is thus today critically challenged by alternative models implying that antibodies cross-react with glomerular antigens ...
... proposes that only those antibodies which cross-reacted with inherent renal antigens induced the organ disease. A nephritogenic potential of antibodies against DNA (or nucleosomes) is thus today critically challenged by alternative models implying that antibodies cross-react with glomerular antigens ...
Cervarix-H-C-721-P46-0088: EPAR - EMA
... and anti HPV-18 antibodies (Cervarix 2-dose schedule divided by Gardasil 2-dose schedule) is above 1. CHMP’s comment The primary objective of the study was met. After Cervarix vaccination as compared to Gardasil vaccination, both administered according to a 2dose schedule in females aged 9-14 years ...
... and anti HPV-18 antibodies (Cervarix 2-dose schedule divided by Gardasil 2-dose schedule) is above 1. CHMP’s comment The primary objective of the study was met. After Cervarix vaccination as compared to Gardasil vaccination, both administered according to a 2dose schedule in females aged 9-14 years ...
Molecular cloning of Per a 1 and definition of the cross
... precursor protein, ANG12, which may be involved in digestion. The Per a 1 cDNA was expressed in Pichia pastoris to produce purified recombinant allergen (yield, 14 mg/L). Conclusion: The results define the molecular structure and antigenic relationships between a new family of cross-reactive “Group ...
... precursor protein, ANG12, which may be involved in digestion. The Per a 1 cDNA was expressed in Pichia pastoris to produce purified recombinant allergen (yield, 14 mg/L). Conclusion: The results define the molecular structure and antigenic relationships between a new family of cross-reactive “Group ...
Antibody
An antibody (Ab), also known as an immunoglobulin (Ig), is a large, Y-shape protein produced by plasma cells that is used by the immune system to identify and neutralize pathogens such as bacteria and viruses. The antibody recognizes a unique molecule of the harmful agent, called an antigen, via the variable region. Each tip of the ""Y"" of an antibody contains a paratope (analogous to a lock) that is specific for one particular epitope (similarly analogous to a key) on an antigen, allowing these two structures to bind together with precision. Using this binding mechanism, an antibody can tag a microbe or an infected cell for attack by other parts of the immune system, or can neutralize its target directly (for example, by blocking a part of a microbe that is essential for its invasion and survival). The ability of an antibody to communicate with the other components of the immune system is mediated via its Fc region (located at the base of the ""Y""), which contains a conserved glycosylation site involved in these interactions. The production of antibodies is the main function of the humoral immune system.Antibodies are secreted by cells of the adaptive immune system (B cells), and more specifically, differentiated B cells called plasma cells. Antibodies can occur in two physical forms, a soluble form that is secreted from the cell, and a membrane-bound form that is attached to the surface of a B cell and is referred to as the B cell receptor (BCR). The BCR is found only on the surface of B cells and facilitates the activation of these cells and their subsequent differentiation into either antibody factories called plasma cells or memory B cells that will survive in the body and remember that same antigen so the B cells can respond faster upon future exposure. In most cases, interaction of the B cell with a T helper cell is necessary to produce full activation of the B cell and, therefore, antibody generation following antigen binding. Soluble antibodies are released into the blood and tissue fluids, as well as many secretions to continue to survey for invading microorganisms.Antibodies are glycoproteins belonging to the immunoglobulin superfamily; the terms antibody and immunoglobulin are often used interchangeably. Though strictly speaking, an antibody is not the same as an immunoglobulin; B cells can produce two types of immunoglobulins - surface immunoglobulins, which are B cell receptors; and secreted immunoglobulins, which are antibodies. So antibodies are one of two classes of immunoglobulins. Antibodies are typically made of basic structural units—each with two large heavy chains and two small light chains. There are several different types of antibody heavy chains based on five different types of crystallisable fragments (Fc) that may be attached to the antigen-binding fragments. The five different types of Fc regions allow antibodies to be grouped into five isotypes. Each Fc region of a particular antibody isotype is able to bind to its specific Fc Receptor (except for IgD, which is essentially the BCR), thus allowing the antigen-antibody complex to mediate different roles depending on which FcR it binds. The ability of an antibody to bind to its corresponding FcR is further modulated by the structure of the glycan(s) present at conserved sites within its Fc region. The ability of antibodies to bind to FcRs helps to direct the appropriate immune response for each different type of foreign object they encounter. For example, IgE is responsible for an allergic response consisting of mast cell degranulation and histamine release. IgE's Fab paratope binds to allergic antigen, for example house dust mite particles, while its Fc region binds to Fc receptor ε. The allergen-IgE-FcRε interaction mediates allergic signal transduction to induce conditions such as asthma. Though the general structure of all antibodies is very similar, a small region at the tip of the protein is extremely variable, allowing millions of antibodies with slightly different tip structures, or antigen-binding sites, to exist. This region is known as the hypervariable region. Each of these variants can bind to a different antigen. This enormous diversity of antibody paratopes on the antigen-binding fragments allows the immune system to recognize an equally wide variety of antigens. The large and diverse population of antibody paratope is generated by random recombination events of a set of gene segments that encode different antigen-binding sites (or paratopes), followed by random mutations in this area of the antibody gene, which create further diversity. This recombinational process that produces clonal antibody paratope diversity is called V(D)J or VJ recombination. Basically, the antibody paratope is polygenic, made up of three genes, V, D, and J. Each paratope locus is also polymorphic, such that during antibody production, one allele of V, one of D, and one of J is chosen. These gene segments are then joined together using random genetic recombination to produce the paratope. The regions where the genes are randomly recombined together is the hyper variable region used to recognise different antigens on a clonal basis. Antibody genes also re-organize in a process called class switching that changes the one type of heavy chain Fc fragment to another, creating a different isotype of the antibody that retains the antigen-specific variable region. This allows a single antibody to be used by different types of Fc receptors, expressed on different parts of the immune system.