Membrane Proteins
... can be exchanged with the solvent outside the cell; Occluded state when the transported ions are enclosed in LeuT; Open to inside when leucine and sodium can be exchanged with the solvent inside the cell. TCA inhibitors lock the transporter in the occluded state (Adapted with permission from Singh S ...
... can be exchanged with the solvent outside the cell; Occluded state when the transported ions are enclosed in LeuT; Open to inside when leucine and sodium can be exchanged with the solvent inside the cell. TCA inhibitors lock the transporter in the occluded state (Adapted with permission from Singh S ...
Three-Point Binding Model
... • Diphosphate must leave • Cannot “see” this step PPi has already left the enzyme site in the crystal structure • However, can use model building to include P & P of ATP: ...
... • Diphosphate must leave • Cannot “see” this step PPi has already left the enzyme site in the crystal structure • However, can use model building to include P & P of ATP: ...
Document
... molecule • Used to bind biotinylated nucleic acids or peptides McDevitt, 1999 Big idea: Streptavidin protein expressed on the cell surface can be used to target any biotinylated DNA/protein to the cell surface. ...
... molecule • Used to bind biotinylated nucleic acids or peptides McDevitt, 1999 Big idea: Streptavidin protein expressed on the cell surface can be used to target any biotinylated DNA/protein to the cell surface. ...
Assignment_week_3_for_AR
... dihydrotestosterone, which are responsible for primary and for secondary male characteristics, respectively. The primary mechanism of action of ARs is by direct regulation of gene transcription. The binding of androgen results in a conformational change in the androgen receptor which causes its tran ...
... dihydrotestosterone, which are responsible for primary and for secondary male characteristics, respectively. The primary mechanism of action of ARs is by direct regulation of gene transcription. The binding of androgen results in a conformational change in the androgen receptor which causes its tran ...
No Slide Title
... component of nickel metabolism. These experiments demonstrate that: • In addition to being a peptidyl-prolyl isomerase, SlyD acts as a protein folding chaperone. • The folding chaperone motif in SlyD and the metal-binding domain are both essential in vivo, whereas the peptidyl-prolyl isomerase activ ...
... component of nickel metabolism. These experiments demonstrate that: • In addition to being a peptidyl-prolyl isomerase, SlyD acts as a protein folding chaperone. • The folding chaperone motif in SlyD and the metal-binding domain are both essential in vivo, whereas the peptidyl-prolyl isomerase activ ...
Model Description Sheet 1
... Primary Citation: Fan, C., Chou, D.Y., Tong, L., Xiang, S. (2012). Crystal structure of urea carboxylase provides insights into the carboxyltransfer reaction. Journal of Biological Chemistry 287:9389-9398. Format: Alpha carbon backbone RP: Zcorp with plaster Description: According to Rice University ...
... Primary Citation: Fan, C., Chou, D.Y., Tong, L., Xiang, S. (2012). Crystal structure of urea carboxylase provides insights into the carboxyltransfer reaction. Journal of Biological Chemistry 287:9389-9398. Format: Alpha carbon backbone RP: Zcorp with plaster Description: According to Rice University ...
NADH-coupled ATPase assay Make the following stock solutions
... If a reaction ends (i.e., runs out of NADH) in < 10 min, it may be approaching the maximal rate of the PK and LDH enzymes, and therefore, they may become rate limiting. In this case, the reaction needs to be adjusted to increase the time domain. If the reaction cannot be slowed, the PK and LDH enz ...
... If a reaction ends (i.e., runs out of NADH) in < 10 min, it may be approaching the maximal rate of the PK and LDH enzymes, and therefore, they may become rate limiting. In this case, the reaction needs to be adjusted to increase the time domain. If the reaction cannot be slowed, the PK and LDH enz ...
Lecture 10 Powerpoint Presentation
... Sensing domain: N-terminal domain that senses external stimuli Usually periplasmic receptor - not always In many cases the ligand or stimulas is unknown Little or no sequence similarity. ...
... Sensing domain: N-terminal domain that senses external stimuli Usually periplasmic receptor - not always In many cases the ligand or stimulas is unknown Little or no sequence similarity. ...
SMART Notebook
... Domain Eukarya- Kingdom Protista Single or simple multicellular Includes: protozoans (animal-like), algae (plant-like), euglena (plant/animal), slime mold ...
... Domain Eukarya- Kingdom Protista Single or simple multicellular Includes: protozoans (animal-like), algae (plant-like), euglena (plant/animal), slime mold ...
Nanodevices
... Nanodevices are found (a) within cell membranes (lipid bilayer), (b) loose in the watery cytoplasm; or (c) clustered in organelles & working together as micromachines Nanodevices are synthesized de novo and then, after varying time, are degraded; these processes require input of energy and a nearly ...
... Nanodevices are found (a) within cell membranes (lipid bilayer), (b) loose in the watery cytoplasm; or (c) clustered in organelles & working together as micromachines Nanodevices are synthesized de novo and then, after varying time, are degraded; these processes require input of energy and a nearly ...
Biological Membranes Transport
... • The main electrogenic pump of plants, fungi, and bacteria is a proton pump ...
... • The main electrogenic pump of plants, fungi, and bacteria is a proton pump ...
chem_1 ILO 2013-9-19 - Faculty Members Websites
... Hemoglobin Transports Oxygen Efficiently by Binding Oxygen cooperatively though non-covalently. Oxygen binding at the Iron sites in Hemoglobin induces substantial changes in quaternary structure of Hemoglobin; Transition from T to R state upon oxygenation Tuning the oxygen affinity of Hemoglob ...
... Hemoglobin Transports Oxygen Efficiently by Binding Oxygen cooperatively though non-covalently. Oxygen binding at the Iron sites in Hemoglobin induces substantial changes in quaternary structure of Hemoglobin; Transition from T to R state upon oxygenation Tuning the oxygen affinity of Hemoglob ...
chem_1 ILO 2013-9-19 - Faculty Members Websites
... Hemoglobin Transports Oxygen Efficiently by Binding Oxygen cooperatively though non-covalently. Oxygen binding at the Iron sites in Hemoglobin induces substantial changes in quaternary structure of Hemoglobin; Transition from T to R state upon oxygenation Tuning the oxygen affinity of Hemoglob ...
... Hemoglobin Transports Oxygen Efficiently by Binding Oxygen cooperatively though non-covalently. Oxygen binding at the Iron sites in Hemoglobin induces substantial changes in quaternary structure of Hemoglobin; Transition from T to R state upon oxygenation Tuning the oxygen affinity of Hemoglob ...
STUMBLING UPON ACTIVE TRANSPORT
... titration of the various components of the reaction. Before this can be done, one must be sure the system is free from outside sources of contamination. In order to study the influence of various cations, including three that are critical for the reaction—Na, K, and Mg2 —Skou had to make sure tha ...
... titration of the various components of the reaction. Before this can be done, one must be sure the system is free from outside sources of contamination. In order to study the influence of various cations, including three that are critical for the reaction—Na, K, and Mg2 —Skou had to make sure tha ...
Special ation Present
... inte Biophysical methods are essential tools during the entire drug discovery process – from early stage R&D through to QC and manufacturing. They can be used for optimization of protein constructs and verification of proper and consistent protein folding, identification of optimal assay buffer, screen ...
... inte Biophysical methods are essential tools during the entire drug discovery process – from early stage R&D through to QC and manufacturing. They can be used for optimization of protein constructs and verification of proper and consistent protein folding, identification of optimal assay buffer, screen ...
Document
... operator sites, O64 and OL. The N-terminal domain mediates the interaction with RNA polymerase (lysogenic promoter for repressor maintenance) and thus, activating the CI gene. (Bell et al.) Therefore, above reasons are why the structure of repressor protein (CI, containing CTD and NTD) is important ...
... operator sites, O64 and OL. The N-terminal domain mediates the interaction with RNA polymerase (lysogenic promoter for repressor maintenance) and thus, activating the CI gene. (Bell et al.) Therefore, above reasons are why the structure of repressor protein (CI, containing CTD and NTD) is important ...
general biology syllabus
... C) Coupled channels: active transport followed by facilitated diffusion 1) Proton pump (proton = H+) a) In photosynthesis and cellular respiration, high-energy e– power first transport protein in active transport of H+ through membrane b) As H+ passes through second membrane protein (passive transpo ...
... C) Coupled channels: active transport followed by facilitated diffusion 1) Proton pump (proton = H+) a) In photosynthesis and cellular respiration, high-energy e– power first transport protein in active transport of H+ through membrane b) As H+ passes through second membrane protein (passive transpo ...
Classification
... MULTIPLE CHOICE: Circle ALL that are TRUE. There may be MORE THAN ONE correct answer. Organisms that can make their own food using chemosynthesis or photosynthesis are called ___________. A. heterotrophic B. autotrophic Organisms whose cells have a nucleus and organelles surrounded by membranes are ...
... MULTIPLE CHOICE: Circle ALL that are TRUE. There may be MORE THAN ONE correct answer. Organisms that can make their own food using chemosynthesis or photosynthesis are called ___________. A. heterotrophic B. autotrophic Organisms whose cells have a nucleus and organelles surrounded by membranes are ...
Classification
... MULTIPLE CHOICE: Circle ALL that are TRUE. There may be MORE THAN ONE correct answer. Organisms that can make their own food using chemosynthesis or photosynthesis are called ___________. A. heterotrophic B. autotrophic Organisms whose cells have a nucleus and organelles surrounded by membranes are ...
... MULTIPLE CHOICE: Circle ALL that are TRUE. There may be MORE THAN ONE correct answer. Organisms that can make their own food using chemosynthesis or photosynthesis are called ___________. A. heterotrophic B. autotrophic Organisms whose cells have a nucleus and organelles surrounded by membranes are ...
Overview of Transport Across Biological Membranes
... Density of carriers will be lower Density of channels will be even lower ...
... Density of carriers will be lower Density of channels will be even lower ...
Transport
... • Include a periplasmic binding protein, a transmembrane channel, and an ATP-hydrolyzing enzyme. • High affinity binding system. • Family of related proteins. – Eubacteria, Archaea, Eukaryotes ...
... • Include a periplasmic binding protein, a transmembrane channel, and an ATP-hydrolyzing enzyme. • High affinity binding system. • Family of related proteins. – Eubacteria, Archaea, Eukaryotes ...
P-type ATPase
The P-type ATPases, also known as E1-E2 ATPases, are a large group of evolutionarily related ion and lipid pumps that are found in bacteria, archaea, and eukaryotes. They are α-helical bundle primary transporters referred to as P-type ATPases because they catalyze auto- (or self-) phosphorylation of a key conserved aspartate residue within the pump. In addition, they all appear to interconvert between at least two different conformations, denoted by E1 and E2.Most members of this transporter family are specific for the pumping of a large array of cations, however one subfamily is involved in flipping phospholipids to maintain the asymmetric nature of the biomembrane.Prominent examples of P-type ATPases are the sodium-potassium pump (Na+,K+-ATPase), the plasma membrane proton pump (H+-ATPase), the proton-potassium pump (H+,K+-ATPase), and the calcium pump (Ca2+-ATPase).