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Functional analysis of the human CDC5L complex
Functional analysis of the human CDC5L complex

... antibody and the protein revealed using ECL (Amersham). Lanes 1 and 2 are duplicates containing supernatants of mock immunodepletions using pre-immune IgG. Lane 3 contains the supernatant from antiCDC5L antibody immunodepleted nuclear extract and lane 4 is similar to lane 3 except that the antibody ...
Poster
Poster

... of the CaaX box motif to Ftase. By inhibiting the farnesylation of the Pre-Lamin A protein, it is mislocalized away from the nucleus. These inhibitors have recently been shown to prevent the formation of misshapen nuclei in mouse fibroblasts containing a targeted progeria syndrome mutation. ...
FEEDING YOUR PET IGUANA
FEEDING YOUR PET IGUANA

... amounts), and flowers such as carnations, hibiscus, and roses (avoid azaleas as they are toxic). Vegetables can be offered cooked or raw (thoroughly wash raw vegetables); experiment with your iguana to see if he prefers his vegetables raw or cooked. Flowers can be home grown or purchased from floral ...
Functional Anthology of Intrinsic Disorder. 1. Biological Processes
Functional Anthology of Intrinsic Disorder. 1. Biological Processes

... disordered proteins/regions revealed that they can be further divided into two groups, random coil-like and pre-molten globule-like conformations.7 Recently, more than 150 proteins have been identified as containing functional disordered regions, or being completely disordered, yet performing vital ...
translation - My Site Dr Neda Bogari
translation - My Site Dr Neda Bogari

... 3. Are there gaps in the code? 4. What are the code words? ...
Translation - Peoria Public Schools / Overview
Translation - Peoria Public Schools / Overview

... Once the mRNA has been synthesized from transcription, it moves to a ribosome in the cytoplasm.  A ribosome consists of two parts, the LARGE SUBUNIT and the SMALL SUBUNIT. These pieces come together during translation.  These subunits are made of protein and ___?____ ...
(PTH) and PTH-Related Protein (PTHrP)
(PTH) and PTH-Related Protein (PTHrP)

... Parathyroid Hormone (PTH) and PTH-Related Protein (PTHrP) Peptides Innovative peptides that bind and activate the PTH/PTHrP receptor (PTH1R). ...
BIOCHEMISTRY Medical biochemistry is an essential component of
BIOCHEMISTRY Medical biochemistry is an essential component of

... II- Classification according to polarity of side chain (R): A- Polar amino acids: in which R contains polar hydrophilic group so can forms hydrogen bond with H2O. In those amino acids, R may contain: 1- OH group : as in serine, threonine and tyrosine 2- SH group : as in cysteine 3- amide group: as i ...
Vasopressin
Vasopressin

... medullary collecting ducts. Description: Cedarlane’s 8-L-Arginine Vasopressin’s molecular weight is 1084.23 Dalton having an amino acid sequence of Cys-Tyr-Phe-Gln-Asn-Cys-Pro-Arg-Gly-NH2 and a molecular formula: C46H65N15O12S2. Presentation: 5 mg (CLHOR280), 25 mg (CLHOR280-2), or 100 mg (CLHOR280- ...
TRANSLATION
TRANSLATION

... 3. Are there gaps in the code? 4. What are the code words? ...
Home Brewing
Home Brewing

... • The glucose is then fermented by the yeast, in an oxygen free environment, into ethyl alcohol ...
A typical hemoglobin molecule consists of a "heme" part and a
A typical hemoglobin molecule consists of a "heme" part and a

... occupied by a nitrogen atom from a histidine side chain on one of the amino acids in the globin. The last coordination site is available to bind an O2 molecule. The heme is therefore the oxygen-carrying portion of the hemoglobin molecules. This raises the question: What is the function of the globul ...
Abstract Example
Abstract Example

... Note: These percentages are not written in stone - they provide a rough guide only. For example, if the work is mostly concerned with a novel methodology, the "Methods" section should be increased accordingly. ...
北京聚合美生物科技有限公司 Mei5 Biotechnology, Co., Ltd M5 GFP
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... localization in vivo. Several GFP mutants have been developed, including EGFP, which fluoresce more intensely than the wildtype GFP and have shifted excitation maxima, making them useful for FACS and fluorescence microscopy as well as double-labeling applications. GFP is widely used in expression ve ...
Amino Acids, Proteins, and Enzymes
Amino Acids, Proteins, and Enzymes

... • Quaternary structure: results from the aggregation (combination) of two or more polypeptide subunits held together by non-covalent interaction like Hbonds, ionic or hydrophobic interactions. • Examples on protein having quaternary structure: – Collagen is a fibrous protein of three polypeptides ( ...
AMINO ACID OVERVIEW There are a total of 20 amino acids
AMINO ACID OVERVIEW There are a total of 20 amino acids

... therefore can get to work faster. This is because the peptide chains are broken up unto smaller fragments outside the body before being ingested. Some gastrointestinal upset can accompany either the ingestion of L-Glutamine or Glutamine peptides. It was a well-known fact that the addition of glutami ...
Picky nascent peptides do not talk to foreign ribosomes
Picky nascent peptides do not talk to foreign ribosomes

... of addressing this question through biochemistry and genetics. One can explore which of the MifM amino acid residues need to be changed to make the peptide recognizable by the E. coli ribosome and what changes need to be introduced in SecM so that it can stall the ribosome from B. subtilis. Converse ...
Sequence elements of the fusion peptide of human respiratory
Sequence elements of the fusion peptide of human respiratory

... HRSV F shares structural features with the F protein of other paramyxoviruses, despite limited sequence identity. Thus, all F proteins have three main hydrophobic regions: one, at the N terminus, which acts as the signal peptide for translocation into the ER; another region, which is the membrane an ...
Identification of Signal Peptides with Sequence Motif Learning
Identification of Signal Peptides with Sequence Motif Learning

... To restate the (-3, -1) rule, the residues at positions –3 and –1 (relative to the cleavage site) must be small and neutral for cleavage to occur correctly. In the previous step, along with the best Z score of a sequence, we also get the most possible starting position of the motif, which gives us a ...
Description of the KDD-Cup 2004 Protein Data
Description of the KDD-Cup 2004 Protein Data

... template structure) using the five similarity measures mentioned above (reference Jaroslaw Meller and Ron Elber, "Linear Optimization and a double Statistical Filter for protein threading protocols", Proteins, Structure, Function and Genetics, 45,241-261(2001) 27-31 Scores of Local threading feature ...
Quantitative Host Cell Protein Analysis Using Two
Quantitative Host Cell Protein Analysis Using Two

... product.10 While quantitative information on the total HCP content provided by ELISA analysis is currently acceptable for lot-release, it does not provide any information on the profile or properties of HCPs present which may be used for process design improvement. Hence methods that facilitate ident ...
Structure-Function studies of novel anticoagulants from snake venoms.
Structure-Function studies of novel anticoagulants from snake venoms.

... We have been involved in isolation and characterization of novel toxins from snake venoms and identified a number of new toxins that belong to known family of toxins, but with different biological properties. We have also identified a few new families of snake venom toxins. The new toxins include (a ...
Determination of Diagnostic Antigens in Cattle Am
Determination of Diagnostic Antigens in Cattle Am

... in Iran including Dicrocoelium dendriticum, Fasciola spp. and hydatid cyst, 7 serum sample representative of each single infection and ten from non infected cattle according to necropsy findings as negative control were collected. Positive sera were pooled by combining equal volumes of sera of cattl ...
CHAPTER FOUR Pseudomonas aeruginosa INFLUENCED PLANKTONIC AND BIOFILM POPULATIONS BASED UPON
CHAPTER FOUR Pseudomonas aeruginosa INFLUENCED PLANKTONIC AND BIOFILM POPULATIONS BASED UPON

... Matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) analyses were performed on a TOF-Spec-2E spectrometer (Micromass, Manchester, UK) equipped with a 337-nm nitrogen laser. The peptide fingerprints were matched against in silico digests using the MS-FIT software with the GenePept ...
Biology PBL
Biology PBL

...  Therefore the chicken chop meal offers an insufficient amount of fat (easily corrected) and carbohydrates. This is essential as the projected intake of carbohydrates is 19% short. This shortage of carbohydrates is important even though the overall calories output of the meal is in the recommended ...
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Protein mass spectrometry



Protein mass spectrometry refers to the application of mass spectrometry to the study of proteins. Mass spectrometry is an important emerging method for the characterization of proteins. The two primary methods for ionization of whole proteins are electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI). In keeping with the performance and mass range of available mass spectrometers, two approaches are used for characterizing proteins. In the first, intact proteins are ionized by either of the two techniques described above, and then introduced to a mass analyzer. This approach is referred to as ""top-down"" strategy of protein analysis. In the second, proteins are enzymatically digested into smaller peptides using a protease such as trypsin. Subsequently these peptides are introduced into the mass spectrometer and identified by peptide mass fingerprinting or tandem mass spectrometry. Hence, this latter approach (also called ""bottom-up"" proteomics) uses identification at the peptide level to infer the existence of proteins.Whole protein mass analysis is primarily conducted using either time-of-flight (TOF) MS, or Fourier transform ion cyclotron resonance (FT-ICR). These two types of instrument are preferable here because of their wide mass range, and in the case of FT-ICR, its high mass accuracy. Mass analysis of proteolytic peptides is a much more popular method of protein characterization, as cheaper instrument designs can be used for characterization. Additionally, sample preparation is easier once whole proteins have been digested into smaller peptide fragments. The most widely used instrument for peptide mass analysis are the MALDI time-of-flight instruments as they permit the acquisition of peptide mass fingerprints (PMFs) at high pace (1 PMF can be analyzed in approx. 10 sec). Multiple stage quadrupole-time-of-flight and the quadrupole ion trap also find use in this application.
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