The split Grainyhead protein of Cnidaria Grainyhead
... Nine motifs were shared between GRH and LSF proteins. Two were unique to LSF. Three were unique to GRH. Based on the motif architecture, the basal metazoan sequences appeared to be bona fide GRH proteins Timothy L. Bailey and Charles Elkan, "Fitting a mixture model by expectation maximization to dis ...
... Nine motifs were shared between GRH and LSF proteins. Two were unique to LSF. Three were unique to GRH. Based on the motif architecture, the basal metazoan sequences appeared to be bona fide GRH proteins Timothy L. Bailey and Charles Elkan, "Fitting a mixture model by expectation maximization to dis ...
The proteome of the infectious bronchitis virus Beau
... To compare whether there were any changes in the groups of proteins that could be identified between uninfected and mock-infected allantoic fluid, lists of proteins identified in each sample were analysed using the PANTHER classification system (Mi et al., 2013) to identify classes of protein functi ...
... To compare whether there were any changes in the groups of proteins that could be identified between uninfected and mock-infected allantoic fluid, lists of proteins identified in each sample were analysed using the PANTHER classification system (Mi et al., 2013) to identify classes of protein functi ...
computer handout - GEP Community Server
... 2. Go to Webct and open the Vibrio sequencing folder. You will randomly select 2 of these sequencing files to analyze. 3. Click on one of the files. It should open in the 4Peaks program. You should see a series of peaks running from left to right, with the base above the peak. Using the blue bar jus ...
... 2. Go to Webct and open the Vibrio sequencing folder. You will randomly select 2 of these sequencing files to analyze. 3. Click on one of the files. It should open in the 4Peaks program. You should see a series of peaks running from left to right, with the base above the peak. Using the blue bar jus ...
Become Hot Spot Healthy
... Coenzyme Q10 which is good for heart muscle. Cooking the carcass to make soup provides hyaluronic acid which is used to make collagen to keep our skin youthful and elastic. • On the other hand, white meat is still meat and eating too much can create acidity, inflammation, and constipation. • In the ...
... Coenzyme Q10 which is good for heart muscle. Cooking the carcass to make soup provides hyaluronic acid which is used to make collagen to keep our skin youthful and elastic. • On the other hand, white meat is still meat and eating too much can create acidity, inflammation, and constipation. • In the ...
Fragment Screening by WAC - Transientic Interactions
... A WACTM column is usually very stable as the immobilized protein is significantly more stable than when present in solution. Proteins such as thrombin and Hsp90 has proven to maintain 90% ...
... A WACTM column is usually very stable as the immobilized protein is significantly more stable than when present in solution. Proteins such as thrombin and Hsp90 has proven to maintain 90% ...
Gene Section SS18 (synovial sarcoma translocation, chromosome 18) Atlas of Genetics and Cytogenetics
... SYT-SSX1, SYT-SSX2, SYT-SSX4; the exon 10 of the SS18 (SYT) gene is fused to exon 6 of the SSX genes. Abnormal Protein The last 8 amino acid residues of the SS18 (SYT) protein are replaced by 78 amino acids from the Cterminal part of SSX proteins. ...
... SYT-SSX1, SYT-SSX2, SYT-SSX4; the exon 10 of the SS18 (SYT) gene is fused to exon 6 of the SSX genes. Abnormal Protein The last 8 amino acid residues of the SS18 (SYT) protein are replaced by 78 amino acids from the Cterminal part of SSX proteins. ...
Rapid Enrichment of Phosphopeptides and Phosphoproteins from
... ions to improve the enzymatic activity of pepsin. Shin et al.20 employed aluminum-impregnated mesoporous silicates to remove small molecules such as fertilizers and detergents containing phosphate groups from wastewater. Lately, Wolschin et al.21 employed Al(OH)3 slurry as adsorbents to extract phos ...
... ions to improve the enzymatic activity of pepsin. Shin et al.20 employed aluminum-impregnated mesoporous silicates to remove small molecules such as fertilizers and detergents containing phosphate groups from wastewater. Lately, Wolschin et al.21 employed Al(OH)3 slurry as adsorbents to extract phos ...
Microscopic Level—The Sarcomere
... Thin filaments, composed of actin, attach to a protein in the Z disc (or Z line) called alpha-actinin, and they are present across the entire length of the I band and a portion of the A band. The region where thick and thin filaments overlap has a dense appearance, as there is little space between ...
... Thin filaments, composed of actin, attach to a protein in the Z disc (or Z line) called alpha-actinin, and they are present across the entire length of the I band and a portion of the A band. The region where thick and thin filaments overlap has a dense appearance, as there is little space between ...
PDF - SAGE Journals
... Methanococcus jannaschii is an autotrophic archaeon originally isolated from an oceanic thermal vent. The primary metabolic pathway for energy production in this hyperthermophilic microbe is methanogenesis from H2 and CO2. As an autotroph, M. jannaschii requires only CO2 as a carbon source for synth ...
... Methanococcus jannaschii is an autotrophic archaeon originally isolated from an oceanic thermal vent. The primary metabolic pathway for energy production in this hyperthermophilic microbe is methanogenesis from H2 and CO2. As an autotroph, M. jannaschii requires only CO2 as a carbon source for synth ...
Villi: Structure and Fun!(ction)
... network of capillaries which surround a specialized lymphatic vessel known as a lacteal. The epithelium of an intestinal villus consists of columnar cells which are covered with microvilli. This succession of folds and projections increases the surface of the intestinal lining for efficient absorpti ...
... network of capillaries which surround a specialized lymphatic vessel known as a lacteal. The epithelium of an intestinal villus consists of columnar cells which are covered with microvilli. This succession of folds and projections increases the surface of the intestinal lining for efficient absorpti ...
CCAO Recruitment Notice 2012 (20 Nov)
... Agilent’s Life Sciences and Applied Group (LSAG) is creating the tools and technologies that are changing the way we understand the world of our customer. Through a comprehensive set of lab productivity and total workflow solutions, LSAG helps scientist advance basic research unlock the genetic caus ...
... Agilent’s Life Sciences and Applied Group (LSAG) is creating the tools and technologies that are changing the way we understand the world of our customer. Through a comprehensive set of lab productivity and total workflow solutions, LSAG helps scientist advance basic research unlock the genetic caus ...
Tay Sachs: A Protein Targeting Disease
... functions. Other proteins are secreted. If proteins end up in the wrong place or don’t get to the right place this can lead to abnormal cell function and/or serious diseases. One means by which the location of proteins is determined is through the process of protein targeting. The mis-sorting of rho ...
... functions. Other proteins are secreted. If proteins end up in the wrong place or don’t get to the right place this can lead to abnormal cell function and/or serious diseases. One means by which the location of proteins is determined is through the process of protein targeting. The mis-sorting of rho ...
Exam Questions_230516_final
... help of dedicated protein-translocation machines, a few proteins can insert into membranes on their own. You are studying a protein that inserts itself into the bacterial membrane independent of the normal translocation machinery. This protein has an N-terminal, 18-amino-acid hydrophilic segment tha ...
... help of dedicated protein-translocation machines, a few proteins can insert into membranes on their own. You are studying a protein that inserts itself into the bacterial membrane independent of the normal translocation machinery. This protein has an N-terminal, 18-amino-acid hydrophilic segment tha ...
The Methylosome, a 20S Complex Containing JBP1 and pICln
... Purification of the SMN complex. For purification of the SMN complex, Flag-Gemin2, myc-SMN, myc-Gemin3, and myc-Gemin4 were transiently expressed in 293 cells, and cytoplasmic extract was prepared from these cells and incubated with anti-Flag Sepharose (Sigma) for 2 h at 4°C. After extensive washing ...
... Purification of the SMN complex. For purification of the SMN complex, Flag-Gemin2, myc-SMN, myc-Gemin3, and myc-Gemin4 were transiently expressed in 293 cells, and cytoplasmic extract was prepared from these cells and incubated with anti-Flag Sepharose (Sigma) for 2 h at 4°C. After extensive washing ...
Architecture of the trypanosome RNA editing accessory complex
... Both MRB8180/MRB4150 and MRB8170/MRB4160 are the result of a chromosomal duplication in T. brucei (but not L. major), and therefore these two proteins have the same single L. major homolog. g Some of the predicted sizes for the T. brucei proteins are different from those listed on TriTrypDB because ...
... Both MRB8180/MRB4150 and MRB8170/MRB4160 are the result of a chromosomal duplication in T. brucei (but not L. major), and therefore these two proteins have the same single L. major homolog. g Some of the predicted sizes for the T. brucei proteins are different from those listed on TriTrypDB because ...
Poster - Physiology and Endocrinology: Male reproduction, deers
... upregulated proteins in the testis of cattle-yak compared with that of yak. Interestingly, approximately 30% of the top 50 downregulated proteins in cattle-yak testis are testis-specific or highly expressed in testis, and most likely exert crucial effects on spermatogenesis. Gene Ontology analysis o ...
... upregulated proteins in the testis of cattle-yak compared with that of yak. Interestingly, approximately 30% of the top 50 downregulated proteins in cattle-yak testis are testis-specific or highly expressed in testis, and most likely exert crucial effects on spermatogenesis. Gene Ontology analysis o ...
(Protein Synthesis) Steps Initiation Elongation Termination
... elongation factor-like protein (EFL) for the same purpose.] It brings the tRNA-AA complex to the large ribosome subunit Aminoacyl (A) site for the tRNA anticodon to pair with the mRNA codon. Once pairing of complementary bases occurs, the EF-1α GTPase domain catalyzes GTP to GDP and EF-1α releases t ...
... elongation factor-like protein (EFL) for the same purpose.] It brings the tRNA-AA complex to the large ribosome subunit Aminoacyl (A) site for the tRNA anticodon to pair with the mRNA codon. Once pairing of complementary bases occurs, the EF-1α GTPase domain catalyzes GTP to GDP and EF-1α releases t ...
Nucleotide sequences of genome segments $8, encoding a capsid
... isolated by HPLC with a reverse-phase column and were subjected to amino acid sequence analysis using automatic protein sequencers (Applied Biosystems, 477A and Shimadzu PSQ-1). As shown in Fig. 1 (a), amino acid sequences of the polypeptide fragments obtained by digestion with trypsin correlated wi ...
... isolated by HPLC with a reverse-phase column and were subjected to amino acid sequence analysis using automatic protein sequencers (Applied Biosystems, 477A and Shimadzu PSQ-1). As shown in Fig. 1 (a), amino acid sequences of the polypeptide fragments obtained by digestion with trypsin correlated wi ...
Nucleotide sequences of genome segments $8, encoding a capsid
... isolated by HPLC with a reverse-phase column and were subjected to amino acid sequence analysis using automatic protein sequencers (Applied Biosystems, 477A and Shimadzu PSQ-1). As shown in Fig. 1 (a), amino acid sequences of the polypeptide fragments obtained by digestion with trypsin correlated wi ...
... isolated by HPLC with a reverse-phase column and were subjected to amino acid sequence analysis using automatic protein sequencers (Applied Biosystems, 477A and Shimadzu PSQ-1). As shown in Fig. 1 (a), amino acid sequences of the polypeptide fragments obtained by digestion with trypsin correlated wi ...
2-D Quant Kit - GE Healthcare Life Sciences
... contaminants in solution. The protein is pelleted by centrifugation and resuspended in an alkaline solution of cupric ions. The cupric ions bind to the polypeptide backbones of any protein present. A colorimetric agent which reacts with unbound cupric ions is then added. The color density is inverse ...
... contaminants in solution. The protein is pelleted by centrifugation and resuspended in an alkaline solution of cupric ions. The cupric ions bind to the polypeptide backbones of any protein present. A colorimetric agent which reacts with unbound cupric ions is then added. The color density is inverse ...
Introduction to Sequence Similarity
... The next few lectures will deal with the topic of “sequence similarity”, where the sequences under consideration might be DNA, RNA, or amino acid sequences. This is likely the most frequently performed task in computational biology. Its usefulness is predicated on the assumption that a high degree o ...
... The next few lectures will deal with the topic of “sequence similarity”, where the sequences under consideration might be DNA, RNA, or amino acid sequences. This is likely the most frequently performed task in computational biology. Its usefulness is predicated on the assumption that a high degree o ...
15 Tacks and a 4 Foot Toober
... • Pairing blue and red tacks (positive and negative charges that neutralize each other), • And pairing green tacks that form disulfide bonds, • And keeping all of the polar white tacks on the surface of the protein. After everyone has folded their toober as best they can, the teacher can point out: ...
... • Pairing blue and red tacks (positive and negative charges that neutralize each other), • And pairing green tacks that form disulfide bonds, • And keeping all of the polar white tacks on the surface of the protein. After everyone has folded their toober as best they can, the teacher can point out: ...
Repeat proteins challenge the concept of structural domains
... Definition of the repeat-units Over the last years, several algorithms have been used to characterize repetitions in protein sequences. Most methods are based on the self-alignment of the primary structure and more sophisticated implementations use spectral analysis of pseudo-chemical characteristics ...
... Definition of the repeat-units Over the last years, several algorithms have been used to characterize repetitions in protein sequences. Most methods are based on the self-alignment of the primary structure and more sophisticated implementations use spectral analysis of pseudo-chemical characteristics ...
File
... the model for a new generation of advanced bio-materials. The new material, biosilk, which has been spun for the first time by researchers at DuPont, has an enormous range of potential uses in construction and manufacturing. B The attraction of the silk spun by the spider is a combination of great s ...
... the model for a new generation of advanced bio-materials. The new material, biosilk, which has been spun for the first time by researchers at DuPont, has an enormous range of potential uses in construction and manufacturing. B The attraction of the silk spun by the spider is a combination of great s ...
Two decades of studying non-covalent biomolecular assemblies by
... development of both technology and methodology. Electrospray ionization (ESI) was an essential development in MS as it allowed the transfer of protein from solution molecules into gas-phase ions. The above studies all employed ESI, which in the late 1980s had been shown to enable the MS analysis of ...
... development of both technology and methodology. Electrospray ionization (ESI) was an essential development in MS as it allowed the transfer of protein from solution molecules into gas-phase ions. The above studies all employed ESI, which in the late 1980s had been shown to enable the MS analysis of ...
Protein mass spectrometry
Protein mass spectrometry refers to the application of mass spectrometry to the study of proteins. Mass spectrometry is an important emerging method for the characterization of proteins. The two primary methods for ionization of whole proteins are electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI). In keeping with the performance and mass range of available mass spectrometers, two approaches are used for characterizing proteins. In the first, intact proteins are ionized by either of the two techniques described above, and then introduced to a mass analyzer. This approach is referred to as ""top-down"" strategy of protein analysis. In the second, proteins are enzymatically digested into smaller peptides using a protease such as trypsin. Subsequently these peptides are introduced into the mass spectrometer and identified by peptide mass fingerprinting or tandem mass spectrometry. Hence, this latter approach (also called ""bottom-up"" proteomics) uses identification at the peptide level to infer the existence of proteins.Whole protein mass analysis is primarily conducted using either time-of-flight (TOF) MS, or Fourier transform ion cyclotron resonance (FT-ICR). These two types of instrument are preferable here because of their wide mass range, and in the case of FT-ICR, its high mass accuracy. Mass analysis of proteolytic peptides is a much more popular method of protein characterization, as cheaper instrument designs can be used for characterization. Additionally, sample preparation is easier once whole proteins have been digested into smaller peptide fragments. The most widely used instrument for peptide mass analysis are the MALDI time-of-flight instruments as they permit the acquisition of peptide mass fingerprints (PMFs) at high pace (1 PMF can be analyzed in approx. 10 sec). Multiple stage quadrupole-time-of-flight and the quadrupole ion trap also find use in this application.