J-Domain Protein CDJ2 and HSP70B Are a Plastidic Chaperone
... to the most conserved proteins known. Except for some Archaea, Hsp70s are found in all known organisms and are present in every compartment of the eukaryotic cell (Bukau and Horwich, 1998). Principally, Hsp70s consist of an Nterminal ATPase domain and a C-terminal substrate-binding domain. ATP hydro ...
... to the most conserved proteins known. Except for some Archaea, Hsp70s are found in all known organisms and are present in every compartment of the eukaryotic cell (Bukau and Horwich, 1998). Principally, Hsp70s consist of an Nterminal ATPase domain and a C-terminal substrate-binding domain. ATP hydro ...
Introduction
... side chains of specific amino acid residues and also can be produced by the corresponding free amino acids. 1.1. Biuret reaction Principle of the method. It is a qualitative reaction of a peptide bond (-CO-NH-). This reaction is produced by any compound having at least 2 peptide linkages in its mole ...
... side chains of specific amino acid residues and also can be produced by the corresponding free amino acids. 1.1. Biuret reaction Principle of the method. It is a qualitative reaction of a peptide bond (-CO-NH-). This reaction is produced by any compound having at least 2 peptide linkages in its mole ...
Chapter 4. Unnatural amino acids with caged side chains
... unnatural amino acid is related to the nature of its side chain, but in a complex fashion. Most investigators have employed relatively small caging groups, because the charged tRNA must pass through the ribosome in order for the amino acid to be incorporated into the receptor. As will be discussed f ...
... unnatural amino acid is related to the nature of its side chain, but in a complex fashion. Most investigators have employed relatively small caging groups, because the charged tRNA must pass through the ribosome in order for the amino acid to be incorporated into the receptor. As will be discussed f ...
Anti-OPN Monoclonal Antibodies as Probes of OPN Structure and
... OVERVIEW: Our characterization of the newer MAbs has used uncloned hybridomas because of our desire to identify MAbs that might not be effectively detected by Western Blotting or ELISAs (because the relevant epitope was bound to the plastic or PVDF membrane). Thus we use two additional screening met ...
... OVERVIEW: Our characterization of the newer MAbs has used uncloned hybridomas because of our desire to identify MAbs that might not be effectively detected by Western Blotting or ELISAs (because the relevant epitope was bound to the plastic or PVDF membrane). Thus we use two additional screening met ...
Proteomics methods for subcellular proteome analysis
... Single-organelle proteomics The combination of MS-based proteomics methods and traditional biochemical fractionation protocols has been a logical step in the characterization of subcellular organization. The protein contents of specific subcellular compartments can thus be identified following speci ...
... Single-organelle proteomics The combination of MS-based proteomics methods and traditional biochemical fractionation protocols has been a logical step in the characterization of subcellular organization. The protein contents of specific subcellular compartments can thus be identified following speci ...
Possible Mechanism of Hepatocyte Injury Induced by
... involvement of the reactive metabolites, Bort et al. (1999) recently reported that diclofenac metabolism into an N-hyReceived for publication July 29, 1999. ...
... involvement of the reactive metabolites, Bort et al. (1999) recently reported that diclofenac metabolism into an N-hyReceived for publication July 29, 1999. ...
Metabolic changes in the glucose-induced apoptotic blastocyst
... experiments was discarded. Previous studies have shown that cells with low ATP undergo necrosis rather then apoptosis, since apoptosis requires energy(6, 15). ATP levels are placed at the outset of each table or figure to demonstrate the equality of the embryos tested. Assay conditions: All assays f ...
... experiments was discarded. Previous studies have shown that cells with low ATP undergo necrosis rather then apoptosis, since apoptosis requires energy(6, 15). ATP levels are placed at the outset of each table or figure to demonstrate the equality of the embryos tested. Assay conditions: All assays f ...
Chapter 5 Endocrine Regulation of Glucose - Rose
... hexokinase reaction is limited by the rate of glucose import into the cell, or by glucose-6-phosphate inhibition of the enzyme. In the liver and pancreas, another enzyme, glucokinase, also catalyzes this reaction. Unlike hexokinase, glucokinase has relatively low affinity for glucose and is regulate ...
... hexokinase reaction is limited by the rate of glucose import into the cell, or by glucose-6-phosphate inhibition of the enzyme. In the liver and pancreas, another enzyme, glucokinase, also catalyzes this reaction. Unlike hexokinase, glucokinase has relatively low affinity for glucose and is regulate ...
ch 9ppt
... The role of glycolysis in oxidizing glucose to two molecules of pyruvate. The process that brings pyruvate from the cytosol into the mitochondria and introduces it into the citric acid cycle. How the process of chemiosmosis utilizes the electrons from NADH and FADH2 to produce ATP. ...
... The role of glycolysis in oxidizing glucose to two molecules of pyruvate. The process that brings pyruvate from the cytosol into the mitochondria and introduces it into the citric acid cycle. How the process of chemiosmosis utilizes the electrons from NADH and FADH2 to produce ATP. ...
respiration revision quiz
... In the reactions of respiration, coenzymes become …………………. as substrates become …………………….. . These are necessary because the reactions are catalysed by inefficient dehydrogenase ………………… . Hydrogen ATO ...
... In the reactions of respiration, coenzymes become …………………. as substrates become …………………….. . These are necessary because the reactions are catalysed by inefficient dehydrogenase ………………… . Hydrogen ATO ...
RBCs metabolism
... • Hemoglobin, the chief protein of the red cells. • Other proteins are present in combination with lipids and oligosaccharide chains, forming the stroma and cell membrane. • Potassium, magnesium, and zinc concentrations in red cells are much higher than in the plasma. ...
... • Hemoglobin, the chief protein of the red cells. • Other proteins are present in combination with lipids and oligosaccharide chains, forming the stroma and cell membrane. • Potassium, magnesium, and zinc concentrations in red cells are much higher than in the plasma. ...
Metabolism of Red Blood Cells (RBCs)
... • Hemoglobin, the chief protein of the red cells. • Other proteins are present in combination with lipids and oligosaccharide chains, forming the stroma and cell membrane. • Potassium, magnesium, and zinc concentrations in red cells are much higher than in the plasma. ...
... • Hemoglobin, the chief protein of the red cells. • Other proteins are present in combination with lipids and oligosaccharide chains, forming the stroma and cell membrane. • Potassium, magnesium, and zinc concentrations in red cells are much higher than in the plasma. ...
Cellular Respiration: Harvesting Chemical Energy
... • The process that generates most of the ATP is called oxidative phosphorylation because it is powered by redox reactions • Oxidative phosphorylation accounts for almost 90% of the ATP generated by cellular respiration • A smaller amount of ATP is formed in glycolysis and the citric acid cycle by s ...
... • The process that generates most of the ATP is called oxidative phosphorylation because it is powered by redox reactions • Oxidative phosphorylation accounts for almost 90% of the ATP generated by cellular respiration • A smaller amount of ATP is formed in glycolysis and the citric acid cycle by s ...
GLUT2 intracytoplasmic loop transmits glucose signaling
... Accumulation of GLUT2 mRNA in mhAT3F cells The transcriptional effect of glucose on two glucose-dependent liver genes, encoding GLUT2 and L-pyruvate kinase, was assayed in clonal stable mhAT3F transfectants (Fig. 2). The cells were cultured in the absence or presence of 17 mM glucose for 24 hours. I ...
... Accumulation of GLUT2 mRNA in mhAT3F cells The transcriptional effect of glucose on two glucose-dependent liver genes, encoding GLUT2 and L-pyruvate kinase, was assayed in clonal stable mhAT3F transfectants (Fig. 2). The cells were cultured in the absence or presence of 17 mM glucose for 24 hours. I ...
A 29 kDa Intracellular Chloride Channel p64H1 Is Associated with
... has been found on several types of vesicles. These channels, acting in concert with the electrogenic proton pump, regulate the pH of the vesicle interior, which is critical for vesicular function. Here we describe the molecular cloning of p64H1, a p64 homolog, from both human and cow. Northern blot ...
... has been found on several types of vesicles. These channels, acting in concert with the electrogenic proton pump, regulate the pH of the vesicle interior, which is critical for vesicular function. Here we describe the molecular cloning of p64H1, a p64 homolog, from both human and cow. Northern blot ...
Corn Syrups: Clearing up the Confusion
... syrup family are made by carefully controlling acid, acid-enzyme or enzyme-enzyme hydrolysis processes. They are differentiated in functionality by assigning each a unique dextrose equivalent (DE) number, a value inversely related to average polymer chain length. By definition, regular corn syrups r ...
... syrup family are made by carefully controlling acid, acid-enzyme or enzyme-enzyme hydrolysis processes. They are differentiated in functionality by assigning each a unique dextrose equivalent (DE) number, a value inversely related to average polymer chain length. By definition, regular corn syrups r ...
Full-Text PDF
... autoinhibitory interaction between the C-terminus and the kinase domain, thereby activating ROCK. Proteolytic cleavage of ROCK by caspases or granzyme B can generate a constitutively active kinase through the removal of the autoinhibitory region. Although less understood, some studies report that RO ...
... autoinhibitory interaction between the C-terminus and the kinase domain, thereby activating ROCK. Proteolytic cleavage of ROCK by caspases or granzyme B can generate a constitutively active kinase through the removal of the autoinhibitory region. Although less understood, some studies report that RO ...
Structural Insights into Kinase Inhibition Ramesh Sistla
... #39-40, KIADB Industrial Area, Electronic City Phase II Bangalore 560 100 ...
... #39-40, KIADB Industrial Area, Electronic City Phase II Bangalore 560 100 ...
... of new drugs and therapeutics (Boguslavsky, 2002 and Marsh, 2002). Affinity binding techniques based on protein fusions have seen rapid acceptance in process laboratories because of their simplicity and the relative ease with which they can be built into expression vectors. Immobilized metal ion aff ...
Metabolic homeostasis of the heart
... and use some allometric relationships to contribute to the analysis. As discussed by Hill for skeletal muscle, it has been appreciated for years that the cardiac concentration of the high energy phosphate molecules of energy metabolism, ATP and CrP, as well as the metabolic intermediates for their f ...
... and use some allometric relationships to contribute to the analysis. As discussed by Hill for skeletal muscle, it has been appreciated for years that the cardiac concentration of the high energy phosphate molecules of energy metabolism, ATP and CrP, as well as the metabolic intermediates for their f ...
The Bacterial Phosphotransferase System: Structure, Function
... E. coli, multiple sites of interaction with the regulatory PTS protein, IIAGlc, account for the allosteric regulation of these transporters. Another very interesting regulatory function of the PTS concerns the control of glycogen metabolism. This topic, with a focus on regulation of the E. coli glyc ...
... E. coli, multiple sites of interaction with the regulatory PTS protein, IIAGlc, account for the allosteric regulation of these transporters. Another very interesting regulatory function of the PTS concerns the control of glycogen metabolism. This topic, with a focus on regulation of the E. coli glyc ...
Aging and Mitochondria - SENS Research Foundation
... – Design and build billions of little machines to do what fibroblast and mesenchymal lineage cells do, only better. – Digest old collagen and synthesize new… in exactly the right pattern. ...
... – Design and build billions of little machines to do what fibroblast and mesenchymal lineage cells do, only better. – Digest old collagen and synthesize new… in exactly the right pattern. ...
Title：利用甜菜根的Arabino-Oligosaccharides(AOS)在試管發酵，選擇
... betalains. The aim of this study was to examine the effect of long term feeding (28 days) with beetroot juice on phase I and phase II enzymes, DNA damage and liver injury induced by hepatocarcinogenic N-nitrosodiethylamine (NDEA). Long term feeding with beetroot juice decreased the activities of enz ...
... betalains. The aim of this study was to examine the effect of long term feeding (28 days) with beetroot juice on phase I and phase II enzymes, DNA damage and liver injury induced by hepatocarcinogenic N-nitrosodiethylamine (NDEA). Long term feeding with beetroot juice decreased the activities of enz ...
Association of CAD, a multifunctional protein involved in pyrimidine
... transfected with or without FLAG-mLST8 vector was subjected to immunoprecipitation with the anti-FLAG antibody, and the proteins eluted from resin with the FLAG peptide were visualized by silver staining after SDS-PAGE. FLAG-mLST8, mTOR, and the proteins recovered from the immunoprecipitate (No.1 to ...
... transfected with or without FLAG-mLST8 vector was subjected to immunoprecipitation with the anti-FLAG antibody, and the proteins eluted from resin with the FLAG peptide were visualized by silver staining after SDS-PAGE. FLAG-mLST8, mTOR, and the proteins recovered from the immunoprecipitate (No.1 to ...
ID_4450_General principles of metaboli_English_sem_5
... They are unstable, free radicals that can react to form toxic substances They polymerize easily and can cause the cytosol to become too gel-like They inhibit the uptake of pyruvate by mitochondria All of the above They are amphipathic and act as detergents that can degrade membranes Why are triacylg ...
... They are unstable, free radicals that can react to form toxic substances They polymerize easily and can cause the cytosol to become too gel-like They inhibit the uptake of pyruvate by mitochondria All of the above They are amphipathic and act as detergents that can degrade membranes Why are triacylg ...
Phosphorylation
Phosphorylation is the addition of a phosphate (PO43−) group to a protein or other organic molecule. Phosphorylation and its counterpart, dephosphorylation, turn many protein enzymes on and off, thereby altering their function and activity. Protein phosphorylation is one type of post-translational modification.Protein phosphorylation in particular plays a significant role in a wide range of cellular processes. Its prominent role in biochemistry is the subject of a very large body of research (as of March 2015, the Medline database returns over 240,000 articles on the subject, largely on protein phosphorylation).