Tetrazine−trans-cyclooctene Mediated Conjugation of Antibodies to
... of the bonds under tension19 as well as at high gliding velocities,20 and reduced velocity of transport.21 The noncovalent biotin−streptavidin interaction is short-ranged and reversible, limiting its practical applications: examples are an almost million fold increase in the dissociation constant (K ...
... of the bonds under tension19 as well as at high gliding velocities,20 and reduced velocity of transport.21 The noncovalent biotin−streptavidin interaction is short-ranged and reversible, limiting its practical applications: examples are an almost million fold increase in the dissociation constant (K ...
Free Full Text ( Final Version , 5mb ) - EUR RePub
... Intracellular catabolism of macromolecules ensures a counterbalance to the pro· cesses of biosynthesis and endocytosis and prevents the improper accumulation of products that would in turn impair the normal physiology of the cell. By means of degradation a cell can remove toxic or damaged components ...
... Intracellular catabolism of macromolecules ensures a counterbalance to the pro· cesses of biosynthesis and endocytosis and prevents the improper accumulation of products that would in turn impair the normal physiology of the cell. By means of degradation a cell can remove toxic or damaged components ...
Pepsin-Mediated Processing of the Cytoplasmic Histone H2A to
... encode antimicrobial peptides is tissue specific. Antimicrobial peptides can function intracellularly, as in circulating leukocytes or in the external environment after release by secretory cells and other granulated epithelia (8). Certain antimicrobial peptides have diverse functions beyond microbi ...
... encode antimicrobial peptides is tissue specific. Antimicrobial peptides can function intracellularly, as in circulating leukocytes or in the external environment after release by secretory cells and other granulated epithelia (8). Certain antimicrobial peptides have diverse functions beyond microbi ...
Structural differences in two biochemically-defined
... complexes situated in crista membranes are affected by membrane conformation. For example, Schàgger17 has described ‘supercomplexes,’ assemblies of the electron-transport chain complexes that require close association to operate at maximum efficiency. The possibility also exists that the biochemical ...
... complexes situated in crista membranes are affected by membrane conformation. For example, Schàgger17 has described ‘supercomplexes,’ assemblies of the electron-transport chain complexes that require close association to operate at maximum efficiency. The possibility also exists that the biochemical ...
Protein Interaction Technical Handbook
... The natural affinity of binding partners for each other is at the core of in vitro methods widely adopted for both interaction discovery and confirmation. In vitro methods span a broad range of techniques. At one end of the range are those methods that can be performed at the bench with basic labora ...
... The natural affinity of binding partners for each other is at the core of in vitro methods widely adopted for both interaction discovery and confirmation. In vitro methods span a broad range of techniques. At one end of the range are those methods that can be performed at the bench with basic labora ...
Platelet isolation and incubation with LDL - HAL
... patients, were the most effective triggers of platelet AA signaling cascade. Although the presence of glucose has been shown to accelerate copper-induced LDL oxidation (17), we did not observe any significant differences between oxidized LDL and glycoxidized LDL in terms of vitamin E and MDA levels. ...
... patients, were the most effective triggers of platelet AA signaling cascade. Although the presence of glucose has been shown to accelerate copper-induced LDL oxidation (17), we did not observe any significant differences between oxidized LDL and glycoxidized LDL in terms of vitamin E and MDA levels. ...
Pericentriolar material structure and dynamics
... in mitosis How does the expansive outer PCM layer assemble around the established inner PCM? Decades of cell biology and molecular genetics have indicated that this process of expansion is driven, in part, by kinase-regulated incorporation of core scaffolding proteins, MT-associated proteins (MAPs) ...
... in mitosis How does the expansive outer PCM layer assemble around the established inner PCM? Decades of cell biology and molecular genetics have indicated that this process of expansion is driven, in part, by kinase-regulated incorporation of core scaffolding proteins, MT-associated proteins (MAPs) ...
curriculum vitae- michael hippler
... Thiol trapping experiments indicate iron-dependent and redox-induced conformational changes in PGRL1 that may interconnect iron metabolism and CEF and thereby partitioning between linear photosynthetic electron transfer and CEF. We propose therefore that PGRL1 in C. reinhardtii possesses a dual func ...
... Thiol trapping experiments indicate iron-dependent and redox-induced conformational changes in PGRL1 that may interconnect iron metabolism and CEF and thereby partitioning between linear photosynthetic electron transfer and CEF. We propose therefore that PGRL1 in C. reinhardtii possesses a dual func ...
Role of Template Activating Factor-I as a
... separated by 10% SDS-PAGE and visualized with Coomassie Brilliant Blue staining. Lane M contains molecular size markers. (B) Formation of histone H1.1NCPs complexes was examined by nucleoprotein gel analyses. The 196 bp 5S rRNA gene fragments or NCPs (0.4 pmol of DNA) assembled on the same DNA with ...
... separated by 10% SDS-PAGE and visualized with Coomassie Brilliant Blue staining. Lane M contains molecular size markers. (B) Formation of histone H1.1NCPs complexes was examined by nucleoprotein gel analyses. The 196 bp 5S rRNA gene fragments or NCPs (0.4 pmol of DNA) assembled on the same DNA with ...
With No Lysine (WNK) Family Proteins and Their
... cysteine. An alternate lysine in β strand 2 functions in its place. The four mammalian genes of the WNK family code for long proteins, 1200 to 2400 amino acids in length. However, outside of the kinase domain, there are no other known folded domains in the protein. There are some conserved areas, na ...
... cysteine. An alternate lysine in β strand 2 functions in its place. The four mammalian genes of the WNK family code for long proteins, 1200 to 2400 amino acids in length. However, outside of the kinase domain, there are no other known folded domains in the protein. There are some conserved areas, na ...
The LIR motif – crucial for selective autophagy
... Fig. 3. LIR motif consensus and structural determinants of LIR–ATG8 interactions. (A) Surface representation of LC3B bound to the p62-LIR peptide (top left), yeast Atg8 bound to the Atg19-LIR peptide (top right), GABARAP-L1 bound to the NBR1-LIR peptide (bottom left) and LC3C bound to the NDP52-LIR ...
... Fig. 3. LIR motif consensus and structural determinants of LIR–ATG8 interactions. (A) Surface representation of LC3B bound to the p62-LIR peptide (top left), yeast Atg8 bound to the Atg19-LIR peptide (top right), GABARAP-L1 bound to the NBR1-LIR peptide (bottom left) and LC3C bound to the NDP52-LIR ...
Dishevelled: at the crossroads of divergent
... contain PDZ domains which can bind to a conserved stretch of amino acids at the C-termini of receptors or form homotypic complexes with other PDZ domains. Multi-domain PDZ domain proteins have been proposed to integrate signaling molecules into larger complexes. For instance, InaD and GRIP can clust ...
... contain PDZ domains which can bind to a conserved stretch of amino acids at the C-termini of receptors or form homotypic complexes with other PDZ domains. Multi-domain PDZ domain proteins have been proposed to integrate signaling molecules into larger complexes. For instance, InaD and GRIP can clust ...
FOCAL ADHESION KINASE: IN COMMAND AND CONTROL OF
... The best-characterized FAK phosphorylation event is AUTOPHOSPHORYLATION at Tyr397, which can occur in either cis or trans 35. Phosphorylation of FAK at Tyr397 creates a motif that is recognized by various SH2-DOMAINcontaining proteins, such as SRC-FAMILY KINASES (SFKs), phospholipase Cγ (PLCγ), supp ...
... The best-characterized FAK phosphorylation event is AUTOPHOSPHORYLATION at Tyr397, which can occur in either cis or trans 35. Phosphorylation of FAK at Tyr397 creates a motif that is recognized by various SH2-DOMAINcontaining proteins, such as SRC-FAMILY KINASES (SFKs), phospholipase Cγ (PLCγ), supp ...
Factor of Transcription by Upstream Binding CAST/hPAF49 Has a
... homologous across the three polymerase classes. Each class has a set of specific subunits, likely targets of their cognate transcription factors. We have identified and characterized a human RNA polymerase I (Pol I)-specific subunit, previously identified as ASE-1 (antisense of ERCC1) and as CD3-as ...
... homologous across the three polymerase classes. Each class has a set of specific subunits, likely targets of their cognate transcription factors. We have identified and characterized a human RNA polymerase I (Pol I)-specific subunit, previously identified as ASE-1 (antisense of ERCC1) and as CD3-as ...
Novel control of lactate dehydrogenase from the freeze
... a crucial role both in sustaining glycolytic ATP production under oxygen-limiting conditions and in facilitating the catabolism of accumulated lactate when stress conditions are relieved. In this study, the effects on LDH of in vivo freezing and dehydration stresses (both of which impose hypoxia/ano ...
... a crucial role both in sustaining glycolytic ATP production under oxygen-limiting conditions and in facilitating the catabolism of accumulated lactate when stress conditions are relieved. In this study, the effects on LDH of in vivo freezing and dehydration stresses (both of which impose hypoxia/ano ...
RNA Polymerase I-Specific Subunit CAST/hPAF49 Has a Role in the
... homologous across the three polymerase classes. Each class has a set of specific subunits, likely targets of their cognate transcription factors. We have identified and characterized a human RNA polymerase I (Pol I)-specific subunit, previously identified as ASE-1 (antisense of ERCC1) and as CD3-as ...
... homologous across the three polymerase classes. Each class has a set of specific subunits, likely targets of their cognate transcription factors. We have identified and characterized a human RNA polymerase I (Pol I)-specific subunit, previously identified as ASE-1 (antisense of ERCC1) and as CD3-as ...
Site-selective incorporation and ligation of
... product were obtained when using RS under more basic conditions ( pH 7.5–8.5 as opposed to pH 6.5).32 Genetic incorporation of a formylglycine tag Alongside the chemical methods described earlier for installing aldehydes into proteins, enzymatic methods, such as the formylglycine tag, have also been ...
... product were obtained when using RS under more basic conditions ( pH 7.5–8.5 as opposed to pH 6.5).32 Genetic incorporation of a formylglycine tag Alongside the chemical methods described earlier for installing aldehydes into proteins, enzymatic methods, such as the formylglycine tag, have also been ...
Characterization of the Cytosolic Proteins Involved in the Amoeboid
... actin, these cells power their movement through modulation of the major sperm protein (MSP) cytoskeleton. MSP forms dense filament meshworks that pack the sperm lamellipod. Protrusion is associated with the assembly of MSP filaments at the leading edge of the lamellipod, and retraction is connected ...
... actin, these cells power their movement through modulation of the major sperm protein (MSP) cytoskeleton. MSP forms dense filament meshworks that pack the sperm lamellipod. Protrusion is associated with the assembly of MSP filaments at the leading edge of the lamellipod, and retraction is connected ...
and Rhizobiales-Like PPP-Family Protein Phosphatases from
... Reversible protein phosphorylation catalyzed by protein kinases and phosphatases represents the most prolific and currently best-characterized post-translational modification. Over the last decade advancements in genome sequencing technologies has massively increased genomic databases, resulting in ...
... Reversible protein phosphorylation catalyzed by protein kinases and phosphatases represents the most prolific and currently best-characterized post-translational modification. Over the last decade advancements in genome sequencing technologies has massively increased genomic databases, resulting in ...
Post-Translational Modifications of the TAK1-TAB Complex
... (NZF) domain of TAB2 and TAB3, which allows the autophosphorylation-dependent activation of TAK1 to occurr after conformational changes [21,23,24]. The structural basis for the interaction between K63-linked polyubiquitin chains and the NZF domains of TAB2 and TAB3 has been determined by crystal str ...
... (NZF) domain of TAB2 and TAB3, which allows the autophosphorylation-dependent activation of TAK1 to occurr after conformational changes [21,23,24]. The structural basis for the interaction between K63-linked polyubiquitin chains and the NZF domains of TAB2 and TAB3 has been determined by crystal str ...
Regulation of Fatty Acid Oxidation by Acetyl
... in cardiac tissues is much higher than that of liver (Cook, 1984). These studies have suggested that activation of PDH by insulin and dichloroacetate (DCA) causes an increase in the malonyl-CoA levels in the cytosol that may lead to inhibition of fatty acid oxidation. Malonyl-CoA is produced from ac ...
... in cardiac tissues is much higher than that of liver (Cook, 1984). These studies have suggested that activation of PDH by insulin and dichloroacetate (DCA) causes an increase in the malonyl-CoA levels in the cytosol that may lead to inhibition of fatty acid oxidation. Malonyl-CoA is produced from ac ...
Biochemistry of Signal Transduction and Regulation - Beck-Shop
... through a number of pathways that receive and process signals originating from the external environment, from other cells within the organism and also from different regions within the cell. In addition to adapting the function of an organism to environmental changes in a signal-directed way, other ...
... through a number of pathways that receive and process signals originating from the external environment, from other cells within the organism and also from different regions within the cell. In addition to adapting the function of an organism to environmental changes in a signal-directed way, other ...
Ornithodoros savignyi CHAPTER 2 SIGNALING PATHWAYS REGULATING PROTEIN SECRETION FROM
... The enhancing effect of GABA on dopamine-stimulated fluid secretion of isolated salivary glands occurred only at high concentrations (Lindsay and Kaufman 1986). Activation of another receptor by GABA may potentiate secretion by another, but poorly understood, mechanism. A model was proposed and expe ...
... The enhancing effect of GABA on dopamine-stimulated fluid secretion of isolated salivary glands occurred only at high concentrations (Lindsay and Kaufman 1986). Activation of another receptor by GABA may potentiate secretion by another, but poorly understood, mechanism. A model was proposed and expe ...
Monoclonal Antibodies Specific for Oxidative Phosphorylation
... Molecular Probes offers a range of subunit-specific antibodies for the study of mitochondrial function and dysfunction (Tables 13 and Figure 1), including monoclonal antibodies specific for subunits of human COX (Complex IV) and for representative subunits of human Complexes I, II, III and V. These ...
... Molecular Probes offers a range of subunit-specific antibodies for the study of mitochondrial function and dysfunction (Tables 13 and Figure 1), including monoclonal antibodies specific for subunits of human COX (Complex IV) and for representative subunits of human Complexes I, II, III and V. These ...
Ageing and the aggregating proteoglycans of
... the internal fragmentation of the link protein. It is possible that this latter type of cleavage may not be due to proteinase action, but may represent the result of reaction with free radicals, as has been postulated for many other age-related changes [87]. ...
... the internal fragmentation of the link protein. It is possible that this latter type of cleavage may not be due to proteinase action, but may represent the result of reaction with free radicals, as has been postulated for many other age-related changes [87]. ...
Phosphorylation
Phosphorylation is the addition of a phosphate (PO43−) group to a protein or other organic molecule. Phosphorylation and its counterpart, dephosphorylation, turn many protein enzymes on and off, thereby altering their function and activity. Protein phosphorylation is one type of post-translational modification.Protein phosphorylation in particular plays a significant role in a wide range of cellular processes. Its prominent role in biochemistry is the subject of a very large body of research (as of March 2015, the Medline database returns over 240,000 articles on the subject, largely on protein phosphorylation).