Stimulation Do Not Alter TTP Function Protein Kinase and
... received the following: 1 g of pGL3 luciferase vector and varying concentrations of the specified pcDNA 3.1 His C-TTP vector, with the difference in DNA transfected made up with the pcDNA3.1 His-C parental for a total of 1 g in 100 l of total, serum free RPMI 1640 medium. The DNA mixture was mixe ...
... received the following: 1 g of pGL3 luciferase vector and varying concentrations of the specified pcDNA 3.1 His C-TTP vector, with the difference in DNA transfected made up with the pcDNA3.1 His-C parental for a total of 1 g in 100 l of total, serum free RPMI 1640 medium. The DNA mixture was mixe ...
Osmo- and thermo-adaptation in hyperthermophilic Archaea
... isolated from a variety of hot environments, most species originate from marine geothermal areas, hence they are slightly halophilic. The accumulation of low-molecular mass organic solutes, i. e., compatible solutes, is one of the most common strategies developed by cells to cope with fluctuations o ...
... isolated from a variety of hot environments, most species originate from marine geothermal areas, hence they are slightly halophilic. The accumulation of low-molecular mass organic solutes, i. e., compatible solutes, is one of the most common strategies developed by cells to cope with fluctuations o ...
Cdc6 in S phase in human cells - Journal of Cell Science
... factors can be lost from isolated nuclei (Kehlenbach et al., 1998) it is likely that nuclear protein export does not function optimally in our system. This has allowed identification of another clear level of regulation (see later). Exogenous Cdc6 does not accumulate in the nucleus in S phase We exa ...
... factors can be lost from isolated nuclei (Kehlenbach et al., 1998) it is likely that nuclear protein export does not function optimally in our system. This has allowed identification of another clear level of regulation (see later). Exogenous Cdc6 does not accumulate in the nucleus in S phase We exa ...
Chromatin Association of Gcn4 Is Limited by Post
... ImageJ quantification software. Antibodies used include rabbit and mouse HA (NEB), Smt3/SUMO (Santa Cruz), and FK1/Ub (Cayman Chemical). ...
... ImageJ quantification software. Antibodies used include rabbit and mouse HA (NEB), Smt3/SUMO (Santa Cruz), and FK1/Ub (Cayman Chemical). ...
Sanguinarine (Pseudochelerythrine) Is a Potent Inhibitor of NF
... unlabeled oligo was added. Also no binding took place when we used a mutant oligonucleotide in which CTC was substituted for GGG at the NF-kB-binding site (Fig. 3B). Sanguinarine Does Not Interfere with the Binding of NF-kB to DNA—It has been shown that both TPCK, a serine protease inhibitor, and he ...
... unlabeled oligo was added. Also no binding took place when we used a mutant oligonucleotide in which CTC was substituted for GGG at the NF-kB-binding site (Fig. 3B). Sanguinarine Does Not Interfere with the Binding of NF-kB to DNA—It has been shown that both TPCK, a serine protease inhibitor, and he ...
The p57 CDKi integrates stress signals into cellcycle progression to
... In contrast, the p57T143A mutant was not phosphorylated by p38 (Figure 3A). Importantly, incubation of the cells with the p38 SAPK inhibitor SB203580 precluded p57 phosphorylation indicating that in vivo p57 phosphorylation required p38 activation (Figure 3B). To rule out that p57 phosphorylation wa ...
... In contrast, the p57T143A mutant was not phosphorylated by p38 (Figure 3A). Importantly, incubation of the cells with the p38 SAPK inhibitor SB203580 precluded p57 phosphorylation indicating that in vivo p57 phosphorylation required p38 activation (Figure 3B). To rule out that p57 phosphorylation wa ...
Nuclear export signal located within the DNAbinding domain of the
... the cytoplasm was comparable to that in untreated cells. The redistribution kinetics of STAT1±GFP were similar in cells not treated with cycloheximide (data not shown). These results suggest that reappearance of STAT1±GFP in the cytoplasm following IFN-g is not a result of newly synthesized protein, ...
... the cytoplasm was comparable to that in untreated cells. The redistribution kinetics of STAT1±GFP were similar in cells not treated with cycloheximide (data not shown). These results suggest that reappearance of STAT1±GFP in the cytoplasm following IFN-g is not a result of newly synthesized protein, ...
Damage response of XRCC1 at sites of DNA single strand breaks is
... cannot repair; pAR localized with CPD at sites of SSBs (Fig. 1C). X1w/oBII dissociated from damage faster and X1 pm was retained at sites of damage longer than full-length XRCC1 (Fig. 1C). pAR could not be detected 10 minutes after UVC irradiation nor could XRCC1BII, supporting the observation that ...
... cannot repair; pAR localized with CPD at sites of SSBs (Fig. 1C). X1w/oBII dissociated from damage faster and X1 pm was retained at sites of damage longer than full-length XRCC1 (Fig. 1C). pAR could not be detected 10 minutes after UVC irradiation nor could XRCC1BII, supporting the observation that ...
Antibody purification handbook
... long serum half-life, low immunogenicity, a high affinity for the antigen, and be able to neutralize the antigen's activity. These are all features that can be enhanced by genetic manipulation. To reduce immunogenicity, mouse-human chimeric antibodies have been produced, containing some human consta ...
... long serum half-life, low immunogenicity, a high affinity for the antigen, and be able to neutralize the antigen's activity. These are all features that can be enhanced by genetic manipulation. To reduce immunogenicity, mouse-human chimeric antibodies have been produced, containing some human consta ...
Sensitive Interaction between Raptor a
... contains an N-terminal RNC (raptor N-terminal conserved) domain found in all its eukaryotic homologs, followed by three HEAT repeats and seven WD-40 repeats in the C-terminal third of the protein (Kim et al., 2002). The mTOR binding site on raptor is not easily defined and, based on mutagenesis and ...
... contains an N-terminal RNC (raptor N-terminal conserved) domain found in all its eukaryotic homologs, followed by three HEAT repeats and seven WD-40 repeats in the C-terminal third of the protein (Kim et al., 2002). The mTOR binding site on raptor is not easily defined and, based on mutagenesis and ...
Protein kinase C- modulates mitochondrial function and active Na
... important target for PKC-⑀ during cardiac ischemia-reperfusion injury. Ischemic preconditioning or phorbol ester induces the translocation of PKC-⑀ to the mitochondria followed by opening of the mitochondrial ATP-sensitive K⫹ channel (37, 45). Active PKC-⑀ also inhibits cytochrome c release from mit ...
... important target for PKC-⑀ during cardiac ischemia-reperfusion injury. Ischemic preconditioning or phorbol ester induces the translocation of PKC-⑀ to the mitochondria followed by opening of the mitochondrial ATP-sensitive K⫹ channel (37, 45). Active PKC-⑀ also inhibits cytochrome c release from mit ...
Identification and characterization of novel interaction
... C-terminal domain of the α subunit of the RNA polymerase C-terminal domain of the α subunit of the RNA polymerase Adenosindiphosphate Ampicillin Adenosinmomophosphate Ammoniumperoxodisulfate Adenosintriphosphate Bacillus base pair bovine serum albumin casamino acids cyclic Adenosinmomophosphate Cata ...
... C-terminal domain of the α subunit of the RNA polymerase C-terminal domain of the α subunit of the RNA polymerase Adenosindiphosphate Ampicillin Adenosinmomophosphate Ammoniumperoxodisulfate Adenosintriphosphate Bacillus base pair bovine serum albumin casamino acids cyclic Adenosinmomophosphate Cata ...
The contractile apparatus and mechanical properties of airway smooth muscle
... transmembrane components of these sites are transmembrane integrins, which attach to ECM proteins at one end and to cytoskeletal proteins at the other end. In nonmuscle cells, several dozen proteins that localize to these membrane adhesion sites have been identified. In smooth muscle, vinculin, tali ...
... transmembrane components of these sites are transmembrane integrins, which attach to ECM proteins at one end and to cytoskeletal proteins at the other end. In nonmuscle cells, several dozen proteins that localize to these membrane adhesion sites have been identified. In smooth muscle, vinculin, tali ...
EphA4 Receptor Forward Signaling Inhibits
... with minimal interference from concurrent changes in insulin secretion. DPHBA has been shown to selectively inhibit EphA2/4 forward signaling through the competitive inhibition of the ligand binding pocket (40). Corresponding to relative levels of EphA4 expression in a- and b-cells, DPHBA treatment ...
... with minimal interference from concurrent changes in insulin secretion. DPHBA has been shown to selectively inhibit EphA2/4 forward signaling through the competitive inhibition of the ligand binding pocket (40). Corresponding to relative levels of EphA4 expression in a- and b-cells, DPHBA treatment ...
The N-Terminal Domain of ERK1 Accounts for the Functional
... is continuously dephosphorylated, the maintenance of a significant pool of active nuclear kinases requires a continuous exchange with the cytosolic fraction [24]. In the present study we demonstrate that differences in the nucleocytoplasmic trafficking of ERK1 and 2 significantly contribute to their ...
... is continuously dephosphorylated, the maintenance of a significant pool of active nuclear kinases requires a continuous exchange with the cytosolic fraction [24]. In the present study we demonstrate that differences in the nucleocytoplasmic trafficking of ERK1 and 2 significantly contribute to their ...
Molecular Genetic Analysis of Phototropism in
... to many different environmental stimuli. One of the regulatory mechanisms involved in these responses is phototropism, which allows plants to change their growth direction in response to the location of the light source. Since the study of phototropism by Darwin, many physiological studies of this p ...
... to many different environmental stimuli. One of the regulatory mechanisms involved in these responses is phototropism, which allows plants to change their growth direction in response to the location of the light source. Since the study of phototropism by Darwin, many physiological studies of this p ...
New insights into regulation of p53 protein degradation
... Modifications of p53 regulates p53 degradation through ubiquitination-dependent proteasome pathway Covalent modifications of p53 occur on more than 40 different amino acid residues and lead to different p53 activation. These modifications include phosphorylation, acetylation, methylation, ubiquitina ...
... Modifications of p53 regulates p53 degradation through ubiquitination-dependent proteasome pathway Covalent modifications of p53 occur on more than 40 different amino acid residues and lead to different p53 activation. These modifications include phosphorylation, acetylation, methylation, ubiquitina ...
The Role of Histone Modifications in Transcriptional Regulation upon Stress
... in the cellular adaptive response upon stress. In this study, we systematically analyze by high throughput screens cellular growth and transcription initiation of stress-responsive genes in 569 histone point mutants upon heat and osmostress. These screens provide a novel global map of the histone re ...
... in the cellular adaptive response upon stress. In this study, we systematically analyze by high throughput screens cellular growth and transcription initiation of stress-responsive genes in 569 histone point mutants upon heat and osmostress. These screens provide a novel global map of the histone re ...
EMD Millipore Protease and Phosphatase Inhibitor Cocktails
... Phosphorylation and dephosphorylation of proteins mediate signal transduction events that control a multitude of cellular processes such as cell division, cell proliferation, and apoptosis. Target proteins are phosphorylated by protein kinases that transfer a phosphate group to a specific protein, t ...
... Phosphorylation and dephosphorylation of proteins mediate signal transduction events that control a multitude of cellular processes such as cell division, cell proliferation, and apoptosis. Target proteins are phosphorylated by protein kinases that transfer a phosphate group to a specific protein, t ...
Acetate formation in the photoheterotrophic bacterium Chloroflexus
... conversion of acetyl-CoA/acetate (acetyl-CoA + ADP + Pi ↔ acetate + ATP + CoA) were determined at 55 °C, pH 7.5. In both directions of the reaction, the rate dependence on the substrate concentrations followed Michaelis–Menten kinetics. The apparent Vmax and Km values in the direction of acetate for ...
... conversion of acetyl-CoA/acetate (acetyl-CoA + ADP + Pi ↔ acetate + ATP + CoA) were determined at 55 °C, pH 7.5. In both directions of the reaction, the rate dependence on the substrate concentrations followed Michaelis–Menten kinetics. The apparent Vmax and Km values in the direction of acetate for ...
Effects of macromolecular crowding on protein folding and
... those found when reduced lysozyme is refolded from acid in the absence of these denaturants (data not shown). This observation excludes prevention of aggregation by low concentrations of denaturants as an explanation for the absence of crowding effects during refolding of oxidized lysozyme, and demo ...
... those found when reduced lysozyme is refolded from acid in the absence of these denaturants (data not shown). This observation excludes prevention of aggregation by low concentrations of denaturants as an explanation for the absence of crowding effects during refolding of oxidized lysozyme, and demo ...
Intermediary metabolism of fructose3
... Downloaded from ajcn.nutrition.org by guest on July 14, 2014 ...
... Downloaded from ajcn.nutrition.org by guest on July 14, 2014 ...
An evolutionarily conserved mechanism for cAMP elicited axonal
... 2015; Hollis and Zou, 2012), Smad1 (Zou et al., 2009), Activin (Omura et al., 2015), HIF-1alpha (Cho et al., 2015) and cAMP (Qiu et al., 2002; Neumann et al., 2002; Cai et al., 1999). Impressively, ectopic elevation of cAMP alone is sufficient to strongly enhance regeneration (Xiao et al., 2015; Qiu ...
... 2015; Hollis and Zou, 2012), Smad1 (Zou et al., 2009), Activin (Omura et al., 2015), HIF-1alpha (Cho et al., 2015) and cAMP (Qiu et al., 2002; Neumann et al., 2002; Cai et al., 1999). Impressively, ectopic elevation of cAMP alone is sufficient to strongly enhance regeneration (Xiao et al., 2015; Qiu ...
PDF - The Journal of Immunology
... encode antimicrobial peptides is tissue specific. Antimicrobial peptides can function intracellularly, as in circulating leukocytes or in the external environment after release by secretory cells and other granulated epithelia (8). Certain antimicrobial peptides have diverse functions beyond microbi ...
... encode antimicrobial peptides is tissue specific. Antimicrobial peptides can function intracellularly, as in circulating leukocytes or in the external environment after release by secretory cells and other granulated epithelia (8). Certain antimicrobial peptides have diverse functions beyond microbi ...
Sucrose metabolism: regulatory mechanisms and pivotal roles in
... these conditions is indicated by the capacity of wildtype plants but not sucrose-synthase-deficient mutants to survive flooding [56,57]. Under pronounced low-oxygen conditions, sucrose synthase responds rapidly to early rises in cytosolic Ca2þ [31,50] and can support considerable biosynthesis (of ce ...
... these conditions is indicated by the capacity of wildtype plants but not sucrose-synthase-deficient mutants to survive flooding [56,57]. Under pronounced low-oxygen conditions, sucrose synthase responds rapidly to early rises in cytosolic Ca2þ [31,50] and can support considerable biosynthesis (of ce ...
Phosphorylation
Phosphorylation is the addition of a phosphate (PO43−) group to a protein or other organic molecule. Phosphorylation and its counterpart, dephosphorylation, turn many protein enzymes on and off, thereby altering their function and activity. Protein phosphorylation is one type of post-translational modification.Protein phosphorylation in particular plays a significant role in a wide range of cellular processes. Its prominent role in biochemistry is the subject of a very large body of research (as of March 2015, the Medline database returns over 240,000 articles on the subject, largely on protein phosphorylation).