willink biochemical genetics unit - Manchester Centre for Genomic
... also be fully identified with the patient name, date of birth, identification number and the date/time of sample collection. All requests must have 3 individual identifiers which will usually be name, date of birth and NHS/hospital number (first name and surname are classed as two identifiers). Requ ...
... also be fully identified with the patient name, date of birth, identification number and the date/time of sample collection. All requests must have 3 individual identifiers which will usually be name, date of birth and NHS/hospital number (first name and surname are classed as two identifiers). Requ ...
Deriving phylogenetic trees from the similarity analysis of metabolic
... result in phylogenetic trees with the wrong topology. The recently completed sequences of several organism genomes provide an enormous amount of data with which to address some of these problems. Phylogenetic analysis can also be performed using the whole genome [14, 29], leading to more precise stu ...
... result in phylogenetic trees with the wrong topology. The recently completed sequences of several organism genomes provide an enormous amount of data with which to address some of these problems. Phylogenetic analysis can also be performed using the whole genome [14, 29], leading to more precise stu ...
Multiomics of tomato glandular trichomes reveals
... water exchange with the outside are limited. ...
... water exchange with the outside are limited. ...
Understanding fatty acid synthesis in developing - Shachar
... must either generate these compounds internally or import them from the cytosol (Browse and Slack, 1985; Hill and Smith, 1991; Smith et al., 1992; Kleppinger-Sparace et al., 1992; Neuhaus et al., 1993; Kang and Rawsthorne, 1996). The ATP necessary for fatty acid synthesis in non-photosynthetic tissu ...
... must either generate these compounds internally or import them from the cytosol (Browse and Slack, 1985; Hill and Smith, 1991; Smith et al., 1992; Kleppinger-Sparace et al., 1992; Neuhaus et al., 1993; Kang and Rawsthorne, 1996). The ATP necessary for fatty acid synthesis in non-photosynthetic tissu ...
Mol. Cell. Proteomics
... Search engine: MS-GF+ v9733 Peptide Variants Modifications: [STY] 80 [M] 16, max PTMs=4 Data: 468 Q-Exactive LC-MS/MS Experiments Site: Broad Institute, TCGA Breast Collection PSM Filters: 0.01 PSM aggregate FDR, 2 spectra per peptide Result: Identified 316,3440 spectra to 291,382 peptide variants ...
... Search engine: MS-GF+ v9733 Peptide Variants Modifications: [STY] 80 [M] 16, max PTMs=4 Data: 468 Q-Exactive LC-MS/MS Experiments Site: Broad Institute, TCGA Breast Collection PSM Filters: 0.01 PSM aggregate FDR, 2 spectra per peptide Result: Identified 316,3440 spectra to 291,382 peptide variants ...
Supporting Information - Royal Society of Chemistry
... substrate (p-nitrophenyl acetate) were first determined (25 mM HEPES buffer, pH = 7.0; absorbance followed at 450 nm). The kinetic data were analyzed via the double reciprocal plots of the initial rates of the enzyme catalyses and the substrate concentrations in the presence of different concentrati ...
... substrate (p-nitrophenyl acetate) were first determined (25 mM HEPES buffer, pH = 7.0; absorbance followed at 450 nm). The kinetic data were analyzed via the double reciprocal plots of the initial rates of the enzyme catalyses and the substrate concentrations in the presence of different concentrati ...
Molecular evolution of the hyaluronan synthase 2 gene in mammals
... Department of Zoology and Entomology, University of Pretoria, Pretoria, South Africa The naked mole-rat (NMR) Heterocephalus glaber is a unique and fascinating mammal exhibiting many unusual adaptations to a subterranean lifestyle. The recent discovery of their resistance to cancer and exceptional l ...
... Department of Zoology and Entomology, University of Pretoria, Pretoria, South Africa The naked mole-rat (NMR) Heterocephalus glaber is a unique and fascinating mammal exhibiting many unusual adaptations to a subterranean lifestyle. The recent discovery of their resistance to cancer and exceptional l ...
Integration of omics data with biochemical reaction
... The appearance of omics data sets has contributed to the rapid development of systems biology, which seeks the understanding of complex biological systems. Constraint-based modeling is one modeling formalism applied in systems biology, which relies on genome-scale network reconstructions. Metabolic ...
... The appearance of omics data sets has contributed to the rapid development of systems biology, which seeks the understanding of complex biological systems. Constraint-based modeling is one modeling formalism applied in systems biology, which relies on genome-scale network reconstructions. Metabolic ...
Determination of Total Homocysteine
... these samples to determine reference ranges for tHCY, MMA, and MCA. Samples obtained from the original NBS specimens of confirmed cases with -cystathionine synthase deficiency (n ⫽ 4), propionylCoA carboxylase deficiency (n ⫽ 2), methylmalonyl-CoA mutase deficiency (n ⫽ 4), Cbl C deficiency (n ⫽ 7) ...
... these samples to determine reference ranges for tHCY, MMA, and MCA. Samples obtained from the original NBS specimens of confirmed cases with -cystathionine synthase deficiency (n ⫽ 4), propionylCoA carboxylase deficiency (n ⫽ 2), methylmalonyl-CoA mutase deficiency (n ⫽ 4), Cbl C deficiency (n ⫽ 7) ...
Metabolic decision making by protein-metabolite - ETH E
... transcriptional regulation sets each promoter’s maximal capacity in a largely growth‐dependent manner. Specific regulation then decides how much of this capacity is being used based on the current demand for the pathway’s end product. In chapter 4, we expand our analysis to quan ...
... transcriptional regulation sets each promoter’s maximal capacity in a largely growth‐dependent manner. Specific regulation then decides how much of this capacity is being used based on the current demand for the pathway’s end product. In chapter 4, we expand our analysis to quan ...
Capabilities and limitations of gel electrophoresis for elemental
... the one hand and electrothermal vaporization—or laser ablation (LA)—inductively coupled plasma–mass spectrometry (ICP–MS) on the other. Keywords: gel electrophoresis; electrothermal vaporization; laser ablation; autoradiography; vanadium; selenium; ICP–mass spectrometry; speciation. INTRODUCTION Sla ...
... the one hand and electrothermal vaporization—or laser ablation (LA)—inductively coupled plasma–mass spectrometry (ICP–MS) on the other. Keywords: gel electrophoresis; electrothermal vaporization; laser ablation; autoradiography; vanadium; selenium; ICP–mass spectrometry; speciation. INTRODUCTION Sla ...
Introduction - MRC Laboratory of Molecular Biology
... Circadian rhythms are cell-autonomous phenomena found throughout biology and have been shown to regulate many aspects of health and disease1. Despite this almost ubiquitous observation of cellular timekeeping, the genes generally proposed to be responsible show little or no homology between kingdoms ...
... Circadian rhythms are cell-autonomous phenomena found throughout biology and have been shown to regulate many aspects of health and disease1. Despite this almost ubiquitous observation of cellular timekeeping, the genes generally proposed to be responsible show little or no homology between kingdoms ...
Nonenzymatic glycolysis and pentose phosphate
... A Spontaneous reactivity of glycolytic and pentose phosphate pathway sugar phosphate intermediates as observed in water. One hundred micromolar of the intermediates (the upper panels illustrate the pentose phosphate pathway, the lower panels the combined pentose phosphate pathway and glycolysis) was ...
... A Spontaneous reactivity of glycolytic and pentose phosphate pathway sugar phosphate intermediates as observed in water. One hundred micromolar of the intermediates (the upper panels illustrate the pentose phosphate pathway, the lower panels the combined pentose phosphate pathway and glycolysis) was ...
Degradation of bidentate coordinated platinum(II)
... reacts the fastest (t1/2, 20 h), followed by 3 (t1/2, 31 h), 2 (t1/2, 40 h), then 1 (t1/2, 68 h), in the order ...
... reacts the fastest (t1/2, 20 h), followed by 3 (t1/2, 31 h), 2 (t1/2, 40 h), then 1 (t1/2, 68 h), in the order ...
Acetic acid - Colorado State University
... With the tunable IR light introduced in the experiment approximately 50 ns prior to the VUV pulse, IR absorption 共multiphoton, most likely兲 by a cluster will induce photodissociation of the neutral 共CH3COOH兲n 共An兲 leading to a reduction of the protonated acetic acid cluster ion 关共CH3COOH兲n−1H+兴 sign ...
... With the tunable IR light introduced in the experiment approximately 50 ns prior to the VUV pulse, IR absorption 共multiphoton, most likely兲 by a cluster will induce photodissociation of the neutral 共CH3COOH兲n 共An兲 leading to a reduction of the protonated acetic acid cluster ion 关共CH3COOH兲n−1H+兴 sign ...
Proficiency Test Lyon 2008
... - 10 splice site mutation in the L2HGDH gene (IVS6+1G>A) and her sister, who died at the age of 11 months after a first episode of gastroenteritis, had the same genotype. L-2-hydroxyglutaric aciduria is a rare inborn error of metabolism (> 100 patients described). The biochemical diagnosis relies o ...
... - 10 splice site mutation in the L2HGDH gene (IVS6+1G>A) and her sister, who died at the age of 11 months after a first episode of gastroenteritis, had the same genotype. L-2-hydroxyglutaric aciduria is a rare inborn error of metabolism (> 100 patients described). The biochemical diagnosis relies o ...
Application Note
... In this application note, the already described HPLC method using 6-aminoquinolyl-Nhydroxysuccinimidyl carbamate (AQC) as the precolumn derivatization reagent is advanced. This highly reactive amine derivatization reagent can be used in an easy one step procedure. The compound reacts with amines thr ...
... In this application note, the already described HPLC method using 6-aminoquinolyl-Nhydroxysuccinimidyl carbamate (AQC) as the precolumn derivatization reagent is advanced. This highly reactive amine derivatization reagent can be used in an easy one step procedure. The compound reacts with amines thr ...
Pan-cancer analysis of the metabolic reaction network
... as a consequence of oncogenic mutations (1, 2). Despite the fact that the regulatory programs ...
... as a consequence of oncogenic mutations (1, 2). Despite the fact that the regulatory programs ...
Novel Class of Heterometallic Cubane and Boride Clusters
... Boron, being the only nonmetal element in group 13, forms a number of different clusters with both main group and transition metal elements. Over the past few decades, extensive efforts have been made to synthesize mixed-metal chalogenide clusters of high nuclearity, and exploitation of new cluster co ...
... Boron, being the only nonmetal element in group 13, forms a number of different clusters with both main group and transition metal elements. Over the past few decades, extensive efforts have been made to synthesize mixed-metal chalogenide clusters of high nuclearity, and exploitation of new cluster co ...
URINE is USEFUL! - Stark, William S.
... • Proteinuria may be the first indication of renovascular, glomerular, or tubulointerstitial renal disease, or may represent the flow of abnormal proteins in the urine in conditions such as multiple myeloma. • Transient proteinuria can be common, especially in the pediatric population and resolves ...
... • Proteinuria may be the first indication of renovascular, glomerular, or tubulointerstitial renal disease, or may represent the flow of abnormal proteins in the urine in conditions such as multiple myeloma. • Transient proteinuria can be common, especially in the pediatric population and resolves ...
Systembiologische Ansätze zur Erforschung des Metabolismus
... • Elementary modes are an appropriate concept to describe biochemical pathways in wild-type and mutants. • Information about network structure can be used to derive far-reaching conclusions about performance of metabolism • Two tendencies in modelling: large-scale vs. mediumscale • Analysis of both ...
... • Elementary modes are an appropriate concept to describe biochemical pathways in wild-type and mutants. • Information about network structure can be used to derive far-reaching conclusions about performance of metabolism • Two tendencies in modelling: large-scale vs. mediumscale • Analysis of both ...
Carbon conversion efficiency and central - Shachar
... embryos (Figure 2b), which was considered in our model by adding a NADP-dependent malic enzyme (EC 1.1.1.40) in the plastid. As previously shown in maize root tips (DieuaideNoubhani et al., 1995), the observation of a substantial loss of label in the C1 carbon position of starch glucosyl units after ...
... embryos (Figure 2b), which was considered in our model by adding a NADP-dependent malic enzyme (EC 1.1.1.40) in the plastid. As previously shown in maize root tips (DieuaideNoubhani et al., 1995), the observation of a substantial loss of label in the C1 carbon position of starch glucosyl units after ...
TD3 5.08 – PKS/NRPS pathways and mass
... “coulombic explosion” (requlsion of charged modecules overcomes droplets surface tension) data is displayed as m/z- mass to charge ratio Ex: insulin has a MV = 5732 Da its specturm has 4 major peaks: 956 (m/6) 1147 (m/5) 1434 (m/4) 1912 (m/3) Software calculates the mass of parent from m/z peaks Eac ...
... “coulombic explosion” (requlsion of charged modecules overcomes droplets surface tension) data is displayed as m/z- mass to charge ratio Ex: insulin has a MV = 5732 Da its specturm has 4 major peaks: 956 (m/6) 1147 (m/5) 1434 (m/4) 1912 (m/3) Software calculates the mass of parent from m/z peaks Eac ...
Title Rapid quantification of tryptophan and tyrosine in
... (Dulbeccos' Modified Eagles Medium), and F12 (a Ham's Nutrient Mixture medium) media, which is often used as a basal medium for mammalian cell fermentation. An enriched basal RDF (eRDF) is formulated by increasing the levels of amino acids and glucose to sustain high density growth. In mammalian cel ...
... (Dulbeccos' Modified Eagles Medium), and F12 (a Ham's Nutrient Mixture medium) media, which is often used as a basal medium for mammalian cell fermentation. An enriched basal RDF (eRDF) is formulated by increasing the levels of amino acids and glucose to sustain high density growth. In mammalian cel ...