Separation of Recombinant Human Erythropoietin (rEPO
... and clinical research use calls for sensitive and analytical techniques. In addition, ...
... and clinical research use calls for sensitive and analytical techniques. In addition, ...
Metabolic profiling during nutrient limited growth in bakers` yeast
... linked to ammonium via glutamine (produced by reaction of ammonia with glutamate) or glutamate (produced by reaction of ammonia with α-ketoglutarate). Glutamine, but not glutamate, was identified as potentially growth-limiting. The growth-limiting pattern of glutamine was strong, highest in the rank ...
... linked to ammonium via glutamine (produced by reaction of ammonia with glutamate) or glutamate (produced by reaction of ammonia with α-ketoglutarate). Glutamine, but not glutamate, was identified as potentially growth-limiting. The growth-limiting pattern of glutamine was strong, highest in the rank ...
Integrating metabolic, transcriptional regulatory and
... large-scale modeling of carbon and energy metabolism is called fluxbalance analysis (FBA). FBA has been used to model metabolism in a host of microbial species, and has been expanded for a variety of applications (reviewed in Price et al., 2004). Two extensions of FBA of interest here are the use of ...
... large-scale modeling of carbon and energy metabolism is called fluxbalance analysis (FBA). FBA has been used to model metabolism in a host of microbial species, and has been expanded for a variety of applications (reviewed in Price et al., 2004). Two extensions of FBA of interest here are the use of ...
New Insight into the Role of the Calvin Cycle: Reutilization
... CO2 fixation was mediated by both the Rubiscos17. These observations strongly suggested some role of the CBB cycle in the heterotrophic P(3HB) biosynthesis from sugars. We assumed that, when the CBB cycle is functional under heterotrophic conditions in the presence of sugars, it may act on fixation ...
... CO2 fixation was mediated by both the Rubiscos17. These observations strongly suggested some role of the CBB cycle in the heterotrophic P(3HB) biosynthesis from sugars. We assumed that, when the CBB cycle is functional under heterotrophic conditions in the presence of sugars, it may act on fixation ...
1 Biosynthesis and Chemical Properties of Natural Substances in
... tissues; however, if these compounds were to exert any toxic effect on the plant, their compartmentation would provide protection against self-poisoning. Membrane targets can be found at the plasma membrane, ER or Golgi apparatus, where the functions of molecules such as transporter and channels, an ...
... tissues; however, if these compounds were to exert any toxic effect on the plant, their compartmentation would provide protection against self-poisoning. Membrane targets can be found at the plasma membrane, ER or Golgi apparatus, where the functions of molecules such as transporter and channels, an ...
msb145697-sup-0001-Supp_Info
... To estimate the fraction of total protein mass covered by mass spectrometry, we rely on two pieces of information: 1) the highly non-uniform distribution of individual protein mass as given by the method of spectral counting (shown above); and 2) the absolute protein quantitation results from the 2D ...
... To estimate the fraction of total protein mass covered by mass spectrometry, we rely on two pieces of information: 1) the highly non-uniform distribution of individual protein mass as given by the method of spectral counting (shown above); and 2) the absolute protein quantitation results from the 2D ...
1 The hydrolysis pattern of procasomorphin by gut proteases from
... steps. This feature is commonly accepted as a prerogative of Fast Atom Bombardment Mass spectrometry and let this technique have a great success in the last 10 years in resolving various kind of problems regarding proteins and peptides [10, 17-22]. On the contrary, even if some examples of sequencin ...
... steps. This feature is commonly accepted as a prerogative of Fast Atom Bombardment Mass spectrometry and let this technique have a great success in the last 10 years in resolving various kind of problems regarding proteins and peptides [10, 17-22]. On the contrary, even if some examples of sequencin ...
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... Urine FeCl3 test and DNPH test used in clinical screening for PKU Blood Phe measurement Urinary pterin analysis by HPLC BH4 loading test PAH gene analysis, common PAH gene mutations are in exon 7 in Chinese PKUs ...
... Urine FeCl3 test and DNPH test used in clinical screening for PKU Blood Phe measurement Urinary pterin analysis by HPLC BH4 loading test PAH gene analysis, common PAH gene mutations are in exon 7 in Chinese PKUs ...
Full text
... according to Varian procedure was prepared by dilution with Milli-Q DI water. Inductively coupled plasma mass spectrometric measurements for multielement determination of trace elements were performed with a Varian 820 –MS (Varian, Australia). The extract solution was introduced by pneumatic nebuliz ...
... according to Varian procedure was prepared by dilution with Milli-Q DI water. Inductively coupled plasma mass spectrometric measurements for multielement determination of trace elements were performed with a Varian 820 –MS (Varian, Australia). The extract solution was introduced by pneumatic nebuliz ...
L-Carnitine in human metabolism
... AND PROTEIN ENZYMES ATP DE-INHIBITION OF ATP-ase IN SARCOLEMMA ...
... AND PROTEIN ENZYMES ATP DE-INHIBITION OF ATP-ase IN SARCOLEMMA ...
University of Birmingham Fundamentals, achievements and
... performed in a microbiology laboratory.1 Therefore, these techniques lack versatility and portability and cannot be used for on-site monitoring.2 In addition, conventional culturing techniques are time-consuming and require trained personnel, thus compromising a timely response to possible outbreaks ...
... performed in a microbiology laboratory.1 Therefore, these techniques lack versatility and portability and cannot be used for on-site monitoring.2 In addition, conventional culturing techniques are time-consuming and require trained personnel, thus compromising a timely response to possible outbreaks ...
Document
... a more systematic manner are STINT-NMR (STructural INTeractions using NMR spectroscopy) and SMILINMR (Small Molecule Inhibitor Library by In-cell NMR). In STINT-NMR, the intracellular binding event of two sequentially expressed polypeptides is observed in vivo (Figure 2b). One of the two proteins is ...
... a more systematic manner are STINT-NMR (STructural INTeractions using NMR spectroscopy) and SMILINMR (Small Molecule Inhibitor Library by In-cell NMR). In STINT-NMR, the intracellular binding event of two sequentially expressed polypeptides is observed in vivo (Figure 2b). One of the two proteins is ...
Selective Amino Acid-Type Labeling(continued)
... undesired amino acids (also called as “isotope scrambling”).3 This happens due to metabolic conversion of one amino acid to another in the bio-synthetic pathway of the cell. The problem becomes more severe for amino acids higher up or intermediates in the metabolic pathway such as Asp, Glu and Gln ( ...
... undesired amino acids (also called as “isotope scrambling”).3 This happens due to metabolic conversion of one amino acid to another in the bio-synthetic pathway of the cell. The problem becomes more severe for amino acids higher up or intermediates in the metabolic pathway such as Asp, Glu and Gln ( ...
Genetic influence on enantiomeric drug disposition: Focus on venlafaxine and citalopram
... of VEN and its three major metabolites was developed and applied in plasma from patients and whole blood samples from forensic autopsy cases. In Papers II and III, the genetic influence on enantiomeric drug disposition in serum and brain following administration of racemic CIT and ...
... of VEN and its three major metabolites was developed and applied in plasma from patients and whole blood samples from forensic autopsy cases. In Papers II and III, the genetic influence on enantiomeric drug disposition in serum and brain following administration of racemic CIT and ...
Unexpected similarities between the
... of the 14 newly identified blood metabolites were methylated or acetylated amino acids. Trimethylated or acetylated free amino acids were also abundant in white blood cells. It may be possible to investigate ...
... of the 14 newly identified blood metabolites were methylated or acetylated amino acids. Trimethylated or acetylated free amino acids were also abundant in white blood cells. It may be possible to investigate ...
Determination of amino acid enantiomers in human urine and blood
... derivatization with OPA together with the chiral thiols Nisobutyryl-L-cysteine and N-isobutyryl-D-cysteine followed by separation of the resulting diastereomeric isoindol derivatives on an ODS stationary phase was reported (Brückner et al., 1994a). After derivatization or urine AAs with 4-fluoro-7- ...
... derivatization with OPA together with the chiral thiols Nisobutyryl-L-cysteine and N-isobutyryl-D-cysteine followed by separation of the resulting diastereomeric isoindol derivatives on an ODS stationary phase was reported (Brückner et al., 1994a). After derivatization or urine AAs with 4-fluoro-7- ...
Get PDF - Wiley Online Library
... Fi3a, Fi3b, and Fi3c, and one that belongs to the T-Superfamily, namely, Fi5a. The other three peptides are contryphans, namely, contryphans fib, fic, and fid. Of these Fi3b, Fi3c, Fi5a, and contryphan fib are novel and are reported for the first time from venom of C. figulinus. The details of the s ...
... Fi3a, Fi3b, and Fi3c, and one that belongs to the T-Superfamily, namely, Fi5a. The other three peptides are contryphans, namely, contryphans fib, fic, and fid. Of these Fi3b, Fi3c, Fi5a, and contryphan fib are novel and are reported for the first time from venom of C. figulinus. The details of the s ...
chromatographic analysis of organic acids, amino acids, and sugars
... Fig. 1) of Ocimum americanum herb the most prevalent was asparagine (353.0 mg/100 g dry weight). It is well known that asparagine is critically required for the development and effective functioning of the human brain (16). γ-Aminobutyric acid, which was also identified (84.0 mg/100 g), is the most ...
... Fig. 1) of Ocimum americanum herb the most prevalent was asparagine (353.0 mg/100 g dry weight). It is well known that asparagine is critically required for the development and effective functioning of the human brain (16). γ-Aminobutyric acid, which was also identified (84.0 mg/100 g), is the most ...
Tools Enabling Metabolic Parents LEarning
... The word mutation means a change or error in the genetic instruction. We inherit particular chromosomes from the egg of the mother and sperm of the father. The genes on those chromosomes carry the instruction that determines characteristics, which are a combination of the parents. ...
... The word mutation means a change or error in the genetic instruction. We inherit particular chromosomes from the egg of the mother and sperm of the father. The genes on those chromosomes carry the instruction that determines characteristics, which are a combination of the parents. ...
Bioenergetics and ioenergetics and Metabolism etabolism
... total energy liberated including heat. As the magnitude of heat energy is small and also that it cannot drive biological reactions, the biochemists are more interested in free energy changes and often use the term exergonic. The corresponding energy-consuming term endergonic refers to the processes ...
... total energy liberated including heat. As the magnitude of heat energy is small and also that it cannot drive biological reactions, the biochemists are more interested in free energy changes and often use the term exergonic. The corresponding energy-consuming term endergonic refers to the processes ...
Effects of Metformin and a Mammalian Target of Rapamycin (mTOR
... targeted LC/MS/MS to characterize the alterations in glycolytic and tricarboxylic acid cycle metabolomics, and employed Western Blot analysis for cell signaling activation by phosphorylation. Comparisons between groups were analyzed using one-way Analysis of Variance followed by secondary post-hoc a ...
... targeted LC/MS/MS to characterize the alterations in glycolytic and tricarboxylic acid cycle metabolomics, and employed Western Blot analysis for cell signaling activation by phosphorylation. Comparisons between groups were analyzed using one-way Analysis of Variance followed by secondary post-hoc a ...
Metabolism—the lost child of cardiology∗
... the heart’s adaptation to pressure overload prevents the adaptive capacity of the organism from being exhausted when environmental requirements are excessive or sustained (25). Part of this adaptive process is a switch from fatty acids to glucose as the main fuel for respiration, which would be a be ...
... the heart’s adaptation to pressure overload prevents the adaptive capacity of the organism from being exhausted when environmental requirements are excessive or sustained (25). Part of this adaptive process is a switch from fatty acids to glucose as the main fuel for respiration, which would be a be ...
Probing the active site of homoserine trans
... was sequenced by MS/MS (Fig. 4). The resulting sequence indicated that the modified lysine in position 45 changed its mass from 228 Da (lysine + 100 Da) to 242 Da (lysine + 100 Da + 14 Da), suggesting the methylation of a single carboxyl group. As succinyl-lysine has one free carboxyl group, the resu ...
... was sequenced by MS/MS (Fig. 4). The resulting sequence indicated that the modified lysine in position 45 changed its mass from 228 Da (lysine + 100 Da) to 242 Da (lysine + 100 Da + 14 Da), suggesting the methylation of a single carboxyl group. As succinyl-lysine has one free carboxyl group, the resu ...
Global Properties of the Metabolic Map of
... competing with its substrates—is defined as a modulator, in the absence of any biological term for this concept.) The information lists all compounds that are known to activate or inhibit an enzyme from in vitro enzymological studies. The database also identifies, for each enzyme, that subset of its ...
... competing with its substrates—is defined as a modulator, in the absence of any biological term for this concept.) The information lists all compounds that are known to activate or inhibit an enzyme from in vitro enzymological studies. The database also identifies, for each enzyme, that subset of its ...