Unraveling Biochemical Pathways Affected by Mitochondrial
... first briefly review the major types of mitochondrial dysfunction(s) with a strong emphasis on mitochondrial uncoupling. We will also discuss the impact of several metabolites on uncoupling proteins’ (UCP) function and expression. We will then summarise the different metabolomic techniques/technolog ...
... first briefly review the major types of mitochondrial dysfunction(s) with a strong emphasis on mitochondrial uncoupling. We will also discuss the impact of several metabolites on uncoupling proteins’ (UCP) function and expression. We will then summarise the different metabolomic techniques/technolog ...
Figure 1 - Drake University
... bupropion concentration, cytosolic protein and incubation time that resulted in linear EBUP and TBUP formation. NADPH dependence was tested in the presence of NADPH regeneration system, NADPH, NADH or no co-factor. All reactions were terminated with a solution of 90% acetonitrile/0.10 M HCl, centrif ...
... bupropion concentration, cytosolic protein and incubation time that resulted in linear EBUP and TBUP formation. NADPH dependence was tested in the presence of NADPH regeneration system, NADPH, NADH or no co-factor. All reactions were terminated with a solution of 90% acetonitrile/0.10 M HCl, centrif ...
Alcohol Metabolism
... o is rate-limiting factor (limits EtOH metabolism to about 8g/h) o leads to competition between EtOH and other metabolic substrates for available NAD+, which may be factor in EtOH-induced liver damage accumulation of acetaldehyde (intermediate metabolite) contributes to hepatotoxicity and is assoc ...
... o is rate-limiting factor (limits EtOH metabolism to about 8g/h) o leads to competition between EtOH and other metabolic substrates for available NAD+, which may be factor in EtOH-induced liver damage accumulation of acetaldehyde (intermediate metabolite) contributes to hepatotoxicity and is assoc ...
Detection of cis-trans Isomers of a Synthetic Peptide Fragment of
... isomers has been confirmed by the CE and NMR studies. It is possible to alter the ratio of the conformers in a reversible manner by heating a solution of the peptide (9–11). With two Pro residues in the peptide a total of four isomers is possible, and this is what was seen with CE. The nature of the ...
... isomers has been confirmed by the CE and NMR studies. It is possible to alter the ratio of the conformers in a reversible manner by heating a solution of the peptide (9–11). With two Pro residues in the peptide a total of four isomers is possible, and this is what was seen with CE. The nature of the ...
HA Convention 2016 Master course How to Handle Abnormal
... Metabolic Screening Results – Causes, Management and Follow up • “Inborn errors of metabolism” (IEM) – a class of genetic disorders with defects of metabolism which are mostly due to single gene defects resulting in defective function of particular enzymes that are essential for conversion of substr ...
... Metabolic Screening Results – Causes, Management and Follow up • “Inborn errors of metabolism” (IEM) – a class of genetic disorders with defects of metabolism which are mostly due to single gene defects resulting in defective function of particular enzymes that are essential for conversion of substr ...
Chronic Lymphocytic Leukemia Cells May Interfere in a Glycated
... from Schleicher & Schuell. Acetonitrile (HPLC grade) was purchased from EM Industries. Trifluoroacetic acid (TFA; photometric grade) was purchased from Aldrich Chemical Co. The blood-spot sample consisted of a single paper disc, 6.3 mm in diameter, punched out from a blood spot dried onto the paper; ...
... from Schleicher & Schuell. Acetonitrile (HPLC grade) was purchased from EM Industries. Trifluoroacetic acid (TFA; photometric grade) was purchased from Aldrich Chemical Co. The blood-spot sample consisted of a single paper disc, 6.3 mm in diameter, punched out from a blood spot dried onto the paper; ...
Metabolic modeling of muscle metabolism identifies key reactions
... Objective: Dysregulated muscle metabolism is a cardinal feature of human insulin resistance (IR) and associated diseases, including type 2 diabetes (T2D). However, specific reactions contributing to abnormal energetics and metabolic inflexibility in IR are unknown. Methods: We utilize flux balance comp ...
... Objective: Dysregulated muscle metabolism is a cardinal feature of human insulin resistance (IR) and associated diseases, including type 2 diabetes (T2D). However, specific reactions contributing to abnormal energetics and metabolic inflexibility in IR are unknown. Methods: We utilize flux balance comp ...
Accurate and Comprehensive Mapping of Multi-omic Data
... Pathway analysis provides a useful biological context for differentially expressed entities resulting from the analysis of high-throughput data in any ‘omics’ (for example genomics, transcriptomics, proteomics, or metabolomics) experiment. Pathways overrepresented or enriched in the entities of inte ...
... Pathway analysis provides a useful biological context for differentially expressed entities resulting from the analysis of high-throughput data in any ‘omics’ (for example genomics, transcriptomics, proteomics, or metabolomics) experiment. Pathways overrepresented or enriched in the entities of inte ...
Environmental enteric dysfunction is associated with carnitine
... storage at − 80 °C until time of analysis. All experimental samples were prepared and analyzed in a masked fashion. Samples were sent in a random order and were prepared at Metabolon, Inc. (Durham, NC) as described elsewhere (Evans et al., 2009). The staff at Metabolon had no access to the study dat ...
... storage at − 80 °C until time of analysis. All experimental samples were prepared and analyzed in a masked fashion. Samples were sent in a random order and were prepared at Metabolon, Inc. (Durham, NC) as described elsewhere (Evans et al., 2009). The staff at Metabolon had no access to the study dat ...
Abstract Here we describe our unprecedented approach in
... The liver samples were subjected to capillary electrophoresis–time of flight-mass spectrometry (CE–TOFMS) and capillary electrophoresis-triple quadrupole mass spectrometry (CE–QqQMS) analyses, and the plasma samples were subjected to CE–TOFMS analysis (Agilent, Palo Alto, CA, USA) as described by So ...
... The liver samples were subjected to capillary electrophoresis–time of flight-mass spectrometry (CE–TOFMS) and capillary electrophoresis-triple quadrupole mass spectrometry (CE–QqQMS) analyses, and the plasma samples were subjected to CE–TOFMS analysis (Agilent, Palo Alto, CA, USA) as described by So ...
Case follow up
... of cecal gas & stool Loss of visualization of tip of liver Paucity of bowel gas (RLQ) ...
... of cecal gas & stool Loss of visualization of tip of liver Paucity of bowel gas (RLQ) ...
M.Sc. Chemistry Syllabus
... UNIT IV : Exclusion Or Gel Chromatography:- Technique in Gel Chromatography, Gel preparation, packing of column, theory and application of gel chromatography UNIT V : H.P.L.C.:- Principle, instrumentation, advantages of HPLC, Effect of temperature in HPLC, HPTLC, Applications of HPLC and HPTLC. UNIT ...
... UNIT IV : Exclusion Or Gel Chromatography:- Technique in Gel Chromatography, Gel preparation, packing of column, theory and application of gel chromatography UNIT V : H.P.L.C.:- Principle, instrumentation, advantages of HPLC, Effect of temperature in HPLC, HPTLC, Applications of HPLC and HPTLC. UNIT ...
Regulation of metabolic pathways at the cellular level
... Compartmentalization of metabolic pathways • Regulation of opposing processes: synthesis of FA takes place in the cytoplasm and their degradation in the mitochondria • Subsequent processes are close to each other (KC and RC) - the local accumulation of substrate • Transport of excess citrate from M ...
... Compartmentalization of metabolic pathways • Regulation of opposing processes: synthesis of FA takes place in the cytoplasm and their degradation in the mitochondria • Subsequent processes are close to each other (KC and RC) - the local accumulation of substrate • Transport of excess citrate from M ...
Guidelines for the Investigation of Hyperammonaemia
... collection or a delay in analysis. (see Appendix –Measurement of Ammonia in Blood/Plasma) Hyperammonaemia can be caused by inherited deficiencies of the enzymes of the urea cycle. They are individually rare disorders but have a combined estimated incidence of approximately 1:30,000. The commonest di ...
... collection or a delay in analysis. (see Appendix –Measurement of Ammonia in Blood/Plasma) Hyperammonaemia can be caused by inherited deficiencies of the enzymes of the urea cycle. They are individually rare disorders but have a combined estimated incidence of approximately 1:30,000. The commonest di ...
Metabolic Adaptation and Protein Complexes in Prokaryotes
... Butland et al. [18] analyzed E. coli complexes using affinity tagged proteins of 1,000 open reading frames (nearly a quarter of the genome). 648 were homogeneously purified and analyzed by mass spectrometry. The direct experimental approach revealed new interactions, as well as interactions predicte ...
... Butland et al. [18] analyzed E. coli complexes using affinity tagged proteins of 1,000 open reading frames (nearly a quarter of the genome). 648 were homogeneously purified and analyzed by mass spectrometry. The direct experimental approach revealed new interactions, as well as interactions predicte ...
The RAVEN Toolbox and Its Use for Generating a Genome
... homology. Previously published GEMs represent a solid basis ...
... homology. Previously published GEMs represent a solid basis ...
Examining Escherichia coli glycolytic pathways, catabolite
... more thermodynamically favorable pathway with fewer enzymatic steps than either the Embden–Meyerhof–Parnas pathway (EMPP) or the oxidative pentose phosphate pathway (OPPP). However, Escherichia coli do not use their native EDP for glucose metabolism. Results: Overexpression of edd and eda in E. col ...
... more thermodynamically favorable pathway with fewer enzymatic steps than either the Embden–Meyerhof–Parnas pathway (EMPP) or the oxidative pentose phosphate pathway (OPPP). However, Escherichia coli do not use their native EDP for glucose metabolism. Results: Overexpression of edd and eda in E. col ...
Biosensor-based engineering of biosynthetic pathways
... By engineering metabolism, we can harness these processes for human needs, such as the industrial production of organic chemicals, fuels, and polymers. Indeed, these microbial metabolic products make up a large and rapidly growing segment of the ‘bioeconomy’ [1,2] (Figure 1a). Despite its value, met ...
... By engineering metabolism, we can harness these processes for human needs, such as the industrial production of organic chemicals, fuels, and polymers. Indeed, these microbial metabolic products make up a large and rapidly growing segment of the ‘bioeconomy’ [1,2] (Figure 1a). Despite its value, met ...
Analysis of Binary Relations and Hierarchies of Enzymes in the
... form. The WWW implementation of KEGG serves several purposes. It allows researchers to examine the functional assignment of enzymes and gives a platform to predict the function of gene products. Visualization of possible metabolic pathways speci c to each organism can be used for the interspecies co ...
... form. The WWW implementation of KEGG serves several purposes. It allows researchers to examine the functional assignment of enzymes and gives a platform to predict the function of gene products. Visualization of possible metabolic pathways speci c to each organism can be used for the interspecies co ...
Metabolic acidosis
... • Lactate generated in muscle goes to liver which converts it to glucose which can be cycled back to muscle (Cori cycle) ...
... • Lactate generated in muscle goes to liver which converts it to glucose which can be cycled back to muscle (Cori cycle) ...
Lecture 10 Mass Spectrommetry Interpretation
... Database search is only good as the database and the input data e.g. MALDI spectra often have peaks due to trypsin autolysis and keratin ...
... Database search is only good as the database and the input data e.g. MALDI spectra often have peaks due to trypsin autolysis and keratin ...
Actin
... Dry-cured ham is a traditional food requiring a long processing period for development of its appreciated texture and flavour characteristics. During this time, there is an intense degradation of muscle proteins due to the action of endogenous proteolytic enzymes. This gives rise to an important gen ...
... Dry-cured ham is a traditional food requiring a long processing period for development of its appreciated texture and flavour characteristics. During this time, there is an intense degradation of muscle proteins due to the action of endogenous proteolytic enzymes. This gives rise to an important gen ...