Host cell protein analysis by mass spectrometry and its application
... HCP analysis in a comparability exercise Case: Exchange of the depth filter in the manufacturing process ELISA results showed comparable levels of HCP in the drug substance batches before and after the filter change ...
... HCP analysis in a comparability exercise Case: Exchange of the depth filter in the manufacturing process ELISA results showed comparable levels of HCP in the drug substance batches before and after the filter change ...
RSC Advances
... Advances in the molecular biology field in the early 1980s resulted in novel approaches for microbial identification and characterization based on polymerase chain reaction (PCR) to amplify specific gene sequences of bacteria. PCR allows in vitro amplification of specific DNA or RNA sequences, the l ...
... Advances in the molecular biology field in the early 1980s resulted in novel approaches for microbial identification and characterization based on polymerase chain reaction (PCR) to amplify specific gene sequences of bacteria. PCR allows in vitro amplification of specific DNA or RNA sequences, the l ...
Insertion of SO2 into the Metal−Carbon Bonds of Rhodium and
... both Cp* and PMe3 resonances. As in the case of the phenylrhodium compound 2a, the PMe3 resonance of 5 (δ 1.79) appears downfield relative to the Cp* peak (δ 1.75) and the CH3 peak appears as a singlet at δ 2.77 ppm, close to a methyl resonance in methane sulfinic acid. These spectroscopic observati ...
... both Cp* and PMe3 resonances. As in the case of the phenylrhodium compound 2a, the PMe3 resonance of 5 (δ 1.79) appears downfield relative to the Cp* peak (δ 1.75) and the CH3 peak appears as a singlet at δ 2.77 ppm, close to a methyl resonance in methane sulfinic acid. These spectroscopic observati ...
Preparation of Human Metabolites of Propranolol Using Laboratory-Evolved Bacterial Cytochromes P450
... Bioconversion of Propranolol by P450 BM3-H 9C1 In a previous study, we created variants of BM3-H with high peroxygenase activity and thermostability by directed evolution, using multiple rounds of mutagenesis and screening (Cirino and Arnold, 2003). In each round, a mutant library created by error-p ...
... Bioconversion of Propranolol by P450 BM3-H 9C1 In a previous study, we created variants of BM3-H with high peroxygenase activity and thermostability by directed evolution, using multiple rounds of mutagenesis and screening (Cirino and Arnold, 2003). In each round, a mutant library created by error-p ...
RSC Advances - thomson mass spectrometry laboratory
... Advances in the molecular biology field in the early 1980s resulted in novel approaches for microbial identification and characterization based on polymerase chain reaction (PCR) to amplify specific gene sequences of bacteria. PCR allows in vitro amplification of specific DNA or RNA sequences, the l ...
... Advances in the molecular biology field in the early 1980s resulted in novel approaches for microbial identification and characterization based on polymerase chain reaction (PCR) to amplify specific gene sequences of bacteria. PCR allows in vitro amplification of specific DNA or RNA sequences, the l ...
Effects of Anions on the Positive Ion Electrospray Ionization Mass
... phase (by positive ion electrospray ionization mass spectrometry) with the net charge residing on the protein in solution will require that the described anion effect be taken into account. In addition, it appears that some control over the distribution of charge states on peptides and protein ions ...
... phase (by positive ion electrospray ionization mass spectrometry) with the net charge residing on the protein in solution will require that the described anion effect be taken into account. In addition, it appears that some control over the distribution of charge states on peptides and protein ions ...
Print this article - Bangladesh Journals Online
... assignable for protons Hd and Ha respectively. The two doublets of doublet at δ 6.5 (JHa-Hb = JHb-Hc = J = 8.0 Hz) and 6.9 (JHb-Hc= JHc-Hd = J = 8.0 Hz) accounts for the Ha and Hd respectively, while the relatively downfield signal at δ 8.5 has been assigned for the imine (=N-H) proton of 2-mercapto ...
... assignable for protons Hd and Ha respectively. The two doublets of doublet at δ 6.5 (JHa-Hb = JHb-Hc = J = 8.0 Hz) and 6.9 (JHb-Hc= JHc-Hd = J = 8.0 Hz) accounts for the Ha and Hd respectively, while the relatively downfield signal at δ 8.5 has been assigned for the imine (=N-H) proton of 2-mercapto ...
NMR Spectroscopy
... •Electronegative groups are "deshielding" and tend to move NMR signals from attached carbons further "downfield" (to higher ppm values). •The -system of alkenes, aromatic compounds and carbonyls strongly deshield C nuclei and move them "downfield" to higher ppm values. •Carbonyl carbons are strongl ...
... •Electronegative groups are "deshielding" and tend to move NMR signals from attached carbons further "downfield" (to higher ppm values). •The -system of alkenes, aromatic compounds and carbonyls strongly deshield C nuclei and move them "downfield" to higher ppm values. •Carbonyl carbons are strongl ...
Peptide fragmentation - University of Szeged, Department of
... composition. Amino acids may form immonium ions with a structure of +NH2=CH-R, where the mass and the stability of the ion depend on the side chain structure [Nomenclature: 26]. Immonium ions sometimes undergo sequential fragmentation reactions yielding ion series characteristic for a particular ami ...
... composition. Amino acids may form immonium ions with a structure of +NH2=CH-R, where the mass and the stability of the ion depend on the side chain structure [Nomenclature: 26]. Immonium ions sometimes undergo sequential fragmentation reactions yielding ion series characteristic for a particular ami ...
Measurement and data processing and analysis
... Measurement and data processing and analysis the visible region. This has allowed us to discover how atoms are bonded in different molecules, and to detect minute quantities of substances in mixtures down to levels of parts per billion. No one method supplies us with all the information we need, so ...
... Measurement and data processing and analysis the visible region. This has allowed us to discover how atoms are bonded in different molecules, and to detect minute quantities of substances in mixtures down to levels of parts per billion. No one method supplies us with all the information we need, so ...
Single Nucleotide Polymorphism (SNP) Genotyping Techniques
... products of the discrimination assay with a matrix compound on a metal plate. The mixture is then heated with a short laser pulse, causing it to expand into the gas phase where ionization is achieved by applying a strong potential difference. Ions are accelerated toward the detector and the time of ...
... products of the discrimination assay with a matrix compound on a metal plate. The mixture is then heated with a short laser pulse, causing it to expand into the gas phase where ionization is achieved by applying a strong potential difference. Ions are accelerated toward the detector and the time of ...
Metabolism of Selenoamino Acids and Contribution of Selenium
... and CySeH is decomposed to H2Se by selenocysteine β-lyase. H2Se is further methylated to MMSe, DMSe and TMSe. It was found that TMSe is produced quickly in mouse liver after oral administration of SeMet, in spite of many metabolic steps from SeMet to CySeH in the methionine-like metabolism. A hypoth ...
... and CySeH is decomposed to H2Se by selenocysteine β-lyase. H2Se is further methylated to MMSe, DMSe and TMSe. It was found that TMSe is produced quickly in mouse liver after oral administration of SeMet, in spite of many metabolic steps from SeMet to CySeH in the methionine-like metabolism. A hypoth ...
Metabolism - Cedarlane
... consistency. With over 300 immunoassay and detection kits, 3,000 antibodies, and a complete set of reagents, Enzo enables sensitive and specific analysis of metabolism biomarkers. ...
... consistency. With over 300 immunoassay and detection kits, 3,000 antibodies, and a complete set of reagents, Enzo enables sensitive and specific analysis of metabolism biomarkers. ...
Making basic science clinically relevant for learners: the biochemistry example Eric Niederhoffer
... Devlin, T. M. (ed.). 2006. Textbook of biochemistry with clinical correlations, 6th ed. John Wiley & Sons, Inc., New York. This is very good for most of what you need. Mehta, A. B., and A. V. Hoffbrand. 2000. Haematology at a glance, Blackwell Science, Malden, Mass. Salway, J. G. 2006. Medical bioch ...
... Devlin, T. M. (ed.). 2006. Textbook of biochemistry with clinical correlations, 6th ed. John Wiley & Sons, Inc., New York. This is very good for most of what you need. Mehta, A. B., and A. V. Hoffbrand. 2000. Haematology at a glance, Blackwell Science, Malden, Mass. Salway, J. G. 2006. Medical bioch ...
ppt
... – Δ: set of possible ion types – m: parent mass – Parameter k (# of mutations/modifications) Output: – A peptide of mass m that is at most k mutations/modifications apart from a database peptide and whose theoretical spectrum matches the experimental S spectrum the best ...
... – Δ: set of possible ion types – m: parent mass – Parameter k (# of mutations/modifications) Output: – A peptide of mass m that is at most k mutations/modifications apart from a database peptide and whose theoretical spectrum matches the experimental S spectrum the best ...
Secondary metabolism is a term for pathways and products
... Metabolites: Metabolites are the intermediates and products of metabolism. The term metabolite is usually restricted to small molecules. A primary metabolite is directly involved in the normal growth, development, and reproduction. A secondary metabolite is not directly involved in those processes, ...
... Metabolites: Metabolites are the intermediates and products of metabolism. The term metabolite is usually restricted to small molecules. A primary metabolite is directly involved in the normal growth, development, and reproduction. A secondary metabolite is not directly involved in those processes, ...
Engineering primary metabolic pathways of industrial
... are most commonly used techniques to create mathematical models. Depending on the method, certain principles need to be taken into account (Voit, 2003). Metabolic flux quantification is a crucial prerequisite to direct as much carbon as possible to the desired product. In the simplest concept of met ...
... are most commonly used techniques to create mathematical models. Depending on the method, certain principles need to be taken into account (Voit, 2003). Metabolic flux quantification is a crucial prerequisite to direct as much carbon as possible to the desired product. In the simplest concept of met ...
Metabolism and Glycolysis
... Glycolysis is an universal metabolic pathway. It occurs, with minor variations, in nearly all organisms, both aerobic and anaerobic. The wide occurrence of glycolysis indicates that it is one of the most ancient known metabolic pathways. In 1860 Louis Pasteur discovered that microorganisms are resp ...
... Glycolysis is an universal metabolic pathway. It occurs, with minor variations, in nearly all organisms, both aerobic and anaerobic. The wide occurrence of glycolysis indicates that it is one of the most ancient known metabolic pathways. In 1860 Louis Pasteur discovered that microorganisms are resp ...
Reversed-Phase High-Performance Liquid Chromatography
... n-alkylsilica supports which can result in low mass yields or the loss of biological activity of larger polypeptides and proteins have often discouraged practitioners from using RP-HPLC methods for large-scale protein separations. The loss of enzymatic activity, the formation of multiple peaks for c ...
... n-alkylsilica supports which can result in low mass yields or the loss of biological activity of larger polypeptides and proteins have often discouraged practitioners from using RP-HPLC methods for large-scale protein separations. The loss of enzymatic activity, the formation of multiple peaks for c ...
Reversed-Phase High-Performance Liquid
... n-alkylsilica supports which can result in low mass yields or the loss of biological activity of larger polypeptides and proteins have often discouraged practitioners from using RP-HPLC methods for large-scale protein separations. The loss of enzymatic activity, the formation of multiple peaks for c ...
... n-alkylsilica supports which can result in low mass yields or the loss of biological activity of larger polypeptides and proteins have often discouraged practitioners from using RP-HPLC methods for large-scale protein separations. The loss of enzymatic activity, the formation of multiple peaks for c ...
A1988L783100001
... even though we used redistilled solvents, antioxidants, and nitrogen atmospheres and carried out the extractions in near darkness in a coldroom. We still have the characteristic optical rotatory dispersion spectrum obtained on that first sample, but we have never managed to repeat that isolation. Pe ...
... even though we used redistilled solvents, antioxidants, and nitrogen atmospheres and carried out the extractions in near darkness in a coldroom. We still have the characteristic optical rotatory dispersion spectrum obtained on that first sample, but we have never managed to repeat that isolation. Pe ...
The road to knowledge: from biology to databases and back again
... HumanCyc enables selecting, for instance, only small molecule reactions. The exact representation of a metabolic reaction differs per database. For example, each database uses a different terminology in its data model to refer to the metabolites before the arrow, e.g., ‘substrates’ or ‘input’, and a ...
... HumanCyc enables selecting, for instance, only small molecule reactions. The exact representation of a metabolic reaction differs per database. For example, each database uses a different terminology in its data model to refer to the metabolites before the arrow, e.g., ‘substrates’ or ‘input’, and a ...
Solid-State and High-Resolution Liquid 119Sn NMR Spectroscopy
... downfield and upfield edges of the observed NMR spectrum in the solid state whereas δ22 represents the frequency that corresponds to a singularity in the powder pattern where the spectral amplitude tends to infinity. Physically, these δnn values describe the shape of an ellipsoid in three dimensions ...
... downfield and upfield edges of the observed NMR spectrum in the solid state whereas δ22 represents the frequency that corresponds to a singularity in the powder pattern where the spectral amplitude tends to infinity. Physically, these δnn values describe the shape of an ellipsoid in three dimensions ...
COPYRIGHTED MATERIAL
... macronutrients is valuable in conditions of altered dietary composition (e.g. low-carbohydrate diets) where a state of energy balance might exist over a few days but be the result of negative carbohydrate balance (using the body’s glycogen reserves to satisfy the brain’s requirement for glucose) mat ...
... macronutrients is valuable in conditions of altered dietary composition (e.g. low-carbohydrate diets) where a state of energy balance might exist over a few days but be the result of negative carbohydrate balance (using the body’s glycogen reserves to satisfy the brain’s requirement for glucose) mat ...
Met1 - Viktor`s Notes for the Neurosurgery Resident
... see p. 750 >> C) purine metabolism disorders - myoadenylate deaminase deficiency see below >> D) mitochondrial myopathies - succinate dehydrogenase deficiency exercise intolerance in childhood; exertion-induced symptoms (muscle pain, weakness, myoglobinuria) in 2-3rd decade. – contractures cause i ...
... see p. 750 >> C) purine metabolism disorders - myoadenylate deaminase deficiency see below >> D) mitochondrial myopathies - succinate dehydrogenase deficiency exercise intolerance in childhood; exertion-induced symptoms (muscle pain, weakness, myoglobinuria) in 2-3rd decade. – contractures cause i ...