In-Cell Solid-State NMR - Institut de Biologie Physico
... versus specific labeling of the alanines) and many parameters were optimized to reduce the intensity of lipid signals versus protein signals. Purified membrane signals were also compared with signals of the same protein reconstituted in detergent micelles. The following year, in-cell 13C and 15N MAS ...
... versus specific labeling of the alanines) and many parameters were optimized to reduce the intensity of lipid signals versus protein signals. Purified membrane signals were also compared with signals of the same protein reconstituted in detergent micelles. The following year, in-cell 13C and 15N MAS ...
Detection of aneuploidy in a single cell using the Ion ReproSeq PGS
... Algorithm parameters have been tuned for low-pass, whole-genome sequencing data using technical sample replicate data as well as samples with known aneuploidies. Users may select from three levels of sensitivity: low, medium, and high. High sensitivity enables more permissive aneuploidy assessment f ...
... Algorithm parameters have been tuned for low-pass, whole-genome sequencing data using technical sample replicate data as well as samples with known aneuploidies. Users may select from three levels of sensitivity: low, medium, and high. High sensitivity enables more permissive aneuploidy assessment f ...
Metabolic production and renal disposal of hydrogen ions
... obvious in the above formulations. adding another 15 mEq of HCI from their metabolism. The total The required destruction of carboxylates occurs in the kidney acid load arising from cationic amino acid metabolism in the during glutamine conversion to uncharged end products, either liver is thus esti ...
... obvious in the above formulations. adding another 15 mEq of HCI from their metabolism. The total The required destruction of carboxylates occurs in the kidney acid load arising from cationic amino acid metabolism in the during glutamine conversion to uncharged end products, either liver is thus esti ...
New Insight into the Main Metabolic Regulation of Escherichia Coli
... Undoubtedly, it is quite important to understand the regulatory processes which govern the cellular metabolism, and this is one of the key focuses in the recent microbial systems biology. Although transcriptional regulation is typically analyzed by measurement of mRNA abundance, the intracellular re ...
... Undoubtedly, it is quite important to understand the regulatory processes which govern the cellular metabolism, and this is one of the key focuses in the recent microbial systems biology. Although transcriptional regulation is typically analyzed by measurement of mRNA abundance, the intracellular re ...
M.Pharm. Branch II - Pharmaceutical Chemistry
... factors affecting chemical shift, spin-spin coupling, coupling constant, and factors influencing the value of coupling constant, spin-spin decoupling, proton exchange reactions, FT-NMR, 2D -NMR, NMDR, NOE, NOESY, COSY and applications in Pharmacy, interpretation of spectra, C13 NMR-Introduction, Nat ...
... factors affecting chemical shift, spin-spin coupling, coupling constant, and factors influencing the value of coupling constant, spin-spin decoupling, proton exchange reactions, FT-NMR, 2D -NMR, NMDR, NOE, NOESY, COSY and applications in Pharmacy, interpretation of spectra, C13 NMR-Introduction, Nat ...
+1/2
... INTEGRATION OF A PEAK Not only does each different type of hydrogen give a distinct peak in the NMR spectrum, but we can also tell the relative numbers of each type of hydrogen by a process called integration. ...
... INTEGRATION OF A PEAK Not only does each different type of hydrogen give a distinct peak in the NMR spectrum, but we can also tell the relative numbers of each type of hydrogen by a process called integration. ...
GeneCensus - Gerstein Lab Publications
... mode consists of a minimal set of enzymes that could operate at steady state with all irreversible reactions proceeding in the appropriate direction and further reduced to omit extraneous metabolites not necessary for the net reaction (25). One should note that there is more than one elementary mode ...
... mode consists of a minimal set of enzymes that could operate at steady state with all irreversible reactions proceeding in the appropriate direction and further reduced to omit extraneous metabolites not necessary for the net reaction (25). One should note that there is more than one elementary mode ...
Predicting the impact of diet and enzymopathies on human small
... absorption of dietary components by the small intestine and to analyze changes in the intestinal metabolism under different genetic backgrounds. We therefore, reconstructed a metabolic network specific for sIECs and subjected it to two different diets to obtain condition-specific metabolic models. S ...
... absorption of dietary components by the small intestine and to analyze changes in the intestinal metabolism under different genetic backgrounds. We therefore, reconstructed a metabolic network specific for sIECs and subjected it to two different diets to obtain condition-specific metabolic models. S ...
Chromatography
... Removal of salts and small molecules from macromolecules can be easily performed by using gel filtration . It is used in the determination of molecular weight of macromolecules . It is used for the purpose of separation of biological molecules leading to their ultimate purification . ...
... Removal of salts and small molecules from macromolecules can be easily performed by using gel filtration . It is used in the determination of molecular weight of macromolecules . It is used for the purpose of separation of biological molecules leading to their ultimate purification . ...
Progress in plant metabolic engineering
... Over the past few years, there has been a growing realization that metabolic pathways must be studied in the context of the whole cell rather than at the single pathway level, and that even the simplest modifications can send ripples throughout the entire system. Attention has therefore shifted away ...
... Over the past few years, there has been a growing realization that metabolic pathways must be studied in the context of the whole cell rather than at the single pathway level, and that even the simplest modifications can send ripples throughout the entire system. Attention has therefore shifted away ...
2nd Amino Acid Workshop - Maastricht Proteomics Center
... LC-MS. The development of modern liquid chromatographic mass spectrometers (LC-MS) dates from the beginning of the 1990s (13). Up until then, all the processes enabling a mass analysis took place in a high vacuum. The development of matrix assisted laser dissociation ionization (MALDI), atmospheric ...
... LC-MS. The development of modern liquid chromatographic mass spectrometers (LC-MS) dates from the beginning of the 1990s (13). Up until then, all the processes enabling a mass analysis took place in a high vacuum. The development of matrix assisted laser dissociation ionization (MALDI), atmospheric ...
Regulation of Primary Metabolism in Response to
... survive short-term hypoxia during flooding or waterlogging, plants must generate sufficient ATP and regenerate NADP+ and NAD+, which are required for glycolysis (Narsai et al., 2011; van Dongen et al., 2011). In addition to the accumulation of ethanol and lactate in oxygen-deprived plant tissues, meta ...
... survive short-term hypoxia during flooding or waterlogging, plants must generate sufficient ATP and regenerate NADP+ and NAD+, which are required for glycolysis (Narsai et al., 2011; van Dongen et al., 2011). In addition to the accumulation of ethanol and lactate in oxygen-deprived plant tissues, meta ...
Supplemental notes in pdf
... body weight due to excess fat stores or a large muscle mass (figure 5). Moreover, BMI values do not provide information about the relative amounts of visceral fat and subcutaneous fat stores. Despite these shortcomings, epidemiological studies have shown that on average, people with a BMI value of > ...
... body weight due to excess fat stores or a large muscle mass (figure 5). Moreover, BMI values do not provide information about the relative amounts of visceral fat and subcutaneous fat stores. Despite these shortcomings, epidemiological studies have shown that on average, people with a BMI value of > ...
PDF
... in the cellular structure, for example, a fixation of enzymes in mitochondria and thus an increased respiratory activity. From these results it seems clear that the period of metamorphosis can be regarded as consisting of three physiologically different parts: first a period of predominating histoly ...
... in the cellular structure, for example, a fixation of enzymes in mitochondria and thus an increased respiratory activity. From these results it seems clear that the period of metamorphosis can be regarded as consisting of three physiologically different parts: first a period of predominating histoly ...
How Toxins Change Metabolism and May Lead to Obesity
... individuality as noted biochemist Dr. Jeremy K. Nicholson of Imperial College London believed that a multitude of factors aside from genetics have a huge influence of how our bodies process medications. • While his study was on the processing of drugs, the ramifications of his paper shows that in th ...
... individuality as noted biochemist Dr. Jeremy K. Nicholson of Imperial College London believed that a multitude of factors aside from genetics have a huge influence of how our bodies process medications. • While his study was on the processing of drugs, the ramifications of his paper shows that in th ...
Table of Available Analyses - NHS Greater Glasgow and Clyde
... Please keep the requests for urgent results to those required only for true clinical emergencies. These requests must be made by a Registrar or Consultant. Do not send “routine” screens, which could be collected the following day. Only by approaching the problem in a spirit of co-operation can t ...
... Please keep the requests for urgent results to those required only for true clinical emergencies. These requests must be made by a Registrar or Consultant. Do not send “routine” screens, which could be collected the following day. Only by approaching the problem in a spirit of co-operation can t ...
Lecture 9 Mass Spectrommetry Techniques
... introduced into trap, collisions will cause peptides to fragment usually the C-N peptide bond to produce b and y ions (see later) -Infrared multiphoton dissociation (IRMPD) – IR laser is fired into ions to excite the vibrations of peptides. -Electron capture dissociation (ECD) – electron beam is fir ...
... introduced into trap, collisions will cause peptides to fragment usually the C-N peptide bond to produce b and y ions (see later) -Infrared multiphoton dissociation (IRMPD) – IR laser is fired into ions to excite the vibrations of peptides. -Electron capture dissociation (ECD) – electron beam is fir ...
Generation of genome-scale metabolic reconstructions for
... human metabolism, which will facilitate studies of host–microbiome interactions. Changes in the composition of the human gut microbiota have been associated with the development of chronic diseases including type 2 diabetes, obesity, and colorectal cancer1. Gut bacterial functions, such as synthesis ...
... human metabolism, which will facilitate studies of host–microbiome interactions. Changes in the composition of the human gut microbiota have been associated with the development of chronic diseases including type 2 diabetes, obesity, and colorectal cancer1. Gut bacterial functions, such as synthesis ...
2 Organic Acidemias
... Developmental delay. The differential diagnosis of developmental delay with other neurologic findings unaccompanied by acidosis or hyperammonemia is extremely long. A high index of suspicion is required to keep an organic acidemia in mind when these symptoms prevail. ...
... Developmental delay. The differential diagnosis of developmental delay with other neurologic findings unaccompanied by acidosis or hyperammonemia is extremely long. A high index of suspicion is required to keep an organic acidemia in mind when these symptoms prevail. ...
Analytical Techniques for Elemental Analysis of Minerals
... on a micrometer scale. Furthermore, quantitative analytical techniques that can be performed at the micrometer scale are preferred. Even when minerals have larger sizes so that sample amounts of 50 mg or even grams are available, there is another problem to consider: such “bulk samples” may contain ...
... on a micrometer scale. Furthermore, quantitative analytical techniques that can be performed at the micrometer scale are preferred. Even when minerals have larger sizes so that sample amounts of 50 mg or even grams are available, there is another problem to consider: such “bulk samples” may contain ...
Embryo metabolism: what does it really mean?
... after blastocyst formation there is a sharp increase in glycolytic ability. The Krebs cycle is the main source of energy throughout the preimplantation period. Large increases in oxygen consumption and uptake and incorporation of carbon occur at about the time of blastocyst formation. The embryo goe ...
... after blastocyst formation there is a sharp increase in glycolytic ability. The Krebs cycle is the main source of energy throughout the preimplantation period. Large increases in oxygen consumption and uptake and incorporation of carbon occur at about the time of blastocyst formation. The embryo goe ...
Electrochromatography
... all polymer molecules of the sample and over time Rg determined experimentally with static light scattering as well as with small angle neutron- and x-ray scattering. The hydrodynamic radius is numerically similar, and can be measured with size exclusion chromatography. Wilkes University - CHM 3 ...
... all polymer molecules of the sample and over time Rg determined experimentally with static light scattering as well as with small angle neutron- and x-ray scattering. The hydrodynamic radius is numerically similar, and can be measured with size exclusion chromatography. Wilkes University - CHM 3 ...
Liquid chromatography: a tool for the analysis of metal species
... sample collection, dissolution, clean-up, trace enrichment and, as previously described, matrix elimination. Sample clean-up can be performed off-line, prior to chromatographic analysis, or on-line, incorporated in the chromatographic hardware. Among the clean-up procedures, those using ion-exchange ...
... sample collection, dissolution, clean-up, trace enrichment and, as previously described, matrix elimination. Sample clean-up can be performed off-line, prior to chromatographic analysis, or on-line, incorporated in the chromatographic hardware. Among the clean-up procedures, those using ion-exchange ...
Reconstruction of biochemical networks in microorganisms
... contains this type of information, and is available for download. ...
... contains this type of information, and is available for download. ...
Discriminating E. coli Isolated from Various Human and Nonhuman
... E. coli isolated from different HM sources (sewage, feces, blood and urine) can be further separated into 3 clusters on the MANOVA plot. Ten sewage isolates not in data base were grouped in HM cluster on MONOVA plot. Seven of the 10 sewage isolates were identified as human isolates based on maximum ...
... E. coli isolated from different HM sources (sewage, feces, blood and urine) can be further separated into 3 clusters on the MANOVA plot. Ten sewage isolates not in data base were grouped in HM cluster on MONOVA plot. Seven of the 10 sewage isolates were identified as human isolates based on maximum ...