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Homology Modelling
Homology Modelling

... CENTER FOR BIOLOGICAL SEQUENCE ANALYSIS TECHNICAL UNIVERSITY OF DENMARK DTU ...
Degradation of fluorobenzene by Rhizobiales strain F11 via
Degradation of fluorobenzene by Rhizobiales strain F11 via

... release was seen only after 29 h (Fig. 1). This indicates that fluorinated intermediates did temporarily accumulate but that there was no formation of high levels of fluorinated dead-end metabolites. Samples (20 l) from the same culture fluid were also analyzed by HPLC and liquid chromatography-mas ...
Flux-balance Analysis - Systems Biology Research Group
Flux-balance Analysis - Systems Biology Research Group

... Systems Biology Research Group http://systemsbiology.ucsd.edu ...
Covalently Bonded Platinum(II) Complexes of [alpha]
Covalently Bonded Platinum(II) Complexes of [alpha]

... are direct consequences of the steric and electronic environment around the observed nuclei, and different values are therefore usually obtained, depending on the R group attached to the organometallic site. Thus, peptide functionalization with these complexes provides a biomarker not only for bioch ...
Analysis of 16 amino acids in tobacco by ion pair
Analysis of 16 amino acids in tobacco by ion pair

...  Amino acids (AA) are chemical markers of tobacco type, maturity, origin...  Two drawbacks for their analysis : lack of chromophoric agents and polarity  Derivation before their detection by UV or fluorescence : This step is often time consuming and can lead to extra errors  No derivation by usi ...
A new metabolomic assay to examine inflammation and redox
A new metabolomic assay to examine inflammation and redox

... The Arg and SAA metabolic pathways are also impacted by metabolism of amino acids that are not directly a part of those pathways. For example, on Arg deficiency, amino acids such as aspartate, glutamate and proline (Figure 1: circles) can be utilized to regenerate Arg [28,29]. Cystathionine (Cysth), ...
Mechanistic model of cardiac energy metabolism predicts
Mechanistic model of cardiac energy metabolism predicts

... are bound together in specific intracellular structures to form a multienzyme complex near the sarcolemma and sarcoplasmic reticulum (8, 19, 42). Because the glycolytic enzymes are not freely distributed, glycolysis can be considered localized in a subdomain within the cytosol. Unfortunately, at pre ...
Alternative isoleucine synthesis pathway in
Alternative isoleucine synthesis pathway in

... experiments are below 0.05 (n52). * represents the labelled carbon. Kdc, 2-keto acid decarboxylase; Adh, alcohol dehydrogenase; LeuABCD, isopropylmalate-related enzymes. The detected labelling patterns in pyruvate, threonine, isoleucine and leucine exclude a biosynthetic pathway via threonine as an ...


... • Sample preparation technique with principles similar to those of HPLC for selective adsorption of analytes or interferences from complex matrices • Used for sample cleanup and analyte concentration preceding LC, GC, ion chromatography and other techniques • Cost-effective alternative to/replacemen ...
The Secrets of Solid Phase Extraction (SPE) for Sample
The Secrets of Solid Phase Extraction (SPE) for Sample

... • Sample preparation technique with principles similar to those of HPLC for selective adsorption of analytes or interferences from complex matrices • Used for sample cleanup and analyte concentration preceding LC, GC, ion chromatography and other techniques • Cost-effective alternative to/replacemen ...
2 Applications
2 Applications

... There are different types of ion sources producing either analyte anions (negative ion mode) or cations (positive ion mode). Generating ions is convenient as they can be efficiently detected and navigated using electric or magnetic fields. The most commonly used ionization methods to generate protei ...
Amino acid analysis in biofluids using LC
Amino acid analysis in biofluids using LC

... under certain conditions (growth, strong physical strain) a number of AA are essential and must be taken by food. AA play an important role in the biochemistry of any living organism. They function as building blocks of proteins and also as energy sources. Some AA in proteins act as catalysts, other ...
HomologyModelling_TB..
HomologyModelling_TB..

... CENTER FOR BIOLOGICAL SEQUENCE ANALYSIS TECHNICAL UNIVERSITY OF DENMARK DTU ...
Secrets from the microbiome: molecular biology meets microbiology
Secrets from the microbiome: molecular biology meets microbiology

... Until recently, the microbiome has been an overlooked entity, largely because 60%-80% of bacterial species could not be cultured. Advances in molecular biology, such as Next Generation Sequencing, have changed that. Bacteria are now typed by sequencing 16SrRNA, a highly conserved molecule with varia ...
Methods and techniques for assessing exposure to antimalarial
Methods and techniques for assessing exposure to antimalarial

... analysis methods between studies. Differences in clinical protocols (e.g. eligibility criteria, standardization of diet, presence of other drugs, comparison groups, source and quality of drug) also contribute to variance in PK estimates. A further limitation is the use of different biological matric ...
A Method To Define the Carboxyl Terminal of Proteins
A Method To Define the Carboxyl Terminal of Proteins

... precursor m/z value for the entire LC-MS/MS run. The m/z values used in these MS/MS analyses were determined by MALDI-MS characterization, initially assuming that the charge state of the peptide ions of interest observed in the electrospray ionization measurement was 2+. For the MS/MS analyses shown ...
Chemistry written examination 1 2008–2012-specifications
Chemistry written examination 1 2008–2012-specifications

... a. Consider the following paragraph. Australian scientists in the forefront of medical research Much research is taking place in Australia into the field of Proteomics. Proteomics is the large scale study of the proteins present in a living organism. The DNA of a cell provides the blueprint for the ...
Semmelweis University Department of Medical Biochemistry
Semmelweis University Department of Medical Biochemistry

... facultative practical exams are graded by 0,1 or 2 points. Only 1 and 2 points are accepted for the semifinal examination. (II)Practical exam as a part of the semifinal examination: Students get one question based on the attached “List of practical questions” and answer it in written form (in 15 min ...
biologically important isotope hybrid
biologically important isotope hybrid

... are intimately connected, probably by the agency of a phenylalanine hydroxylase. Figure 9 illustrates the pmr spectrum of 2H-cytochrome c ('H-leucine) in the oxidized form. In comparison to the spectra of Figure 1, a very simple pattern indeed is obtained. Three methyl groups (tentative assignment) ...
Methyl Jasmonate-Elicited Transcriptional
Methyl Jasmonate-Elicited Transcriptional

... medicine for the treatment of various ailments. Although a variety of secondary metabolites with potent biological activities are identified, our understanding of the biosynthetic pathways that produce them has remained largely incomplete. We studied transcriptional changes in sweet basil after methy ...
Carnitine-acylcarnitine translocase deficiency: metabolic
Carnitine-acylcarnitine translocase deficiency: metabolic

... esters were prepared and FAB-MS was done using methanol–glycerol–3nitrobenzylalcohol (2:1:1, v / v / v) as matrix. Quantitative analysis of plasma acylcarnitines was performed by electrospray tandem mass spectrometry (ESI-MS–MS) using a modification of the method of Chace et al. [18]. A 3.0 mm-diame ...
iRsp1095: A genome-scale reconstruction of the Rhodobacter
iRsp1095: A genome-scale reconstruction of the Rhodobacter

... produce large amounts of carotenoids or isoprenoids as a source of biocommodities, H2 as a potential biofuel, or polyhydroxybutyrate (PHB) as raw material for biodegradable plastics [6]. Furthermore, the autotrophic metabolism of R. sphaeroides makes it a potential organism for use in the synthesis ...
Ammonia (plasma, blood) - Association for Clinical Biochemistry
Ammonia (plasma, blood) - Association for Clinical Biochemistry

... a specimen tube (preferably pre‐chilled) containing either lithium  heparin or EDTA as an anticoagulant and which has been determined to  be free of ammonia contamination. Ideally, the patient should be non‐ stressed, as difficult venepuncture can cause a spurious increase in  [ammonia].     The sam ...
Determination and changes of free amino acids in royal
Determination and changes of free amino acids in royal

... volatility of their derivatives. These compounds eluted very early in gas chromatography and their rsd decreased with the increasing GC retention time. The recovery efficiency of the clean-up procedure was also evaluated by performing the complete analysis on three replications of the standard mixtu ...
Localized in vivo 13C-NMR of Glutamate Metabolism in the Human
Localized in vivo 13C-NMR of Glutamate Metabolism in the Human

... with a standard clinical body gradient coil and amplifier (Siemens AS25, Erlangen, Germany). The magnet and gradient system was interfaced with a spectrometer console (Varian, Palo Alto, Calif., USA) using a manufacturer-supplied interface board. Subjects were positioned supine on the patient bed ab ...
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Metabolomics

Metabolomics is the scientific study of chemical processes involving metabolites. Specifically, metabolomics is the ""systematic study of the unique chemical fingerprints that specific cellular processes leave behind"", the study of their small-molecule metabolite profiles. The metabolome represents the collection of all metabolites in a biological cell, tissue, organ or organism, which are the end products of cellular processes. mRNA gene expression data and proteomic analyses reveal the set of gene products being produced in the cell, data that represents one aspect of cellular function. Conversely, metabolic profiling can give an instantaneous snapshot of the physiology of that cell. One of the challenges of systems biology and functional genomics is to integrate proteomic, transcriptomic, and metabolomic information to provide a better understanding of cellular biology.
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