A role for the Drosophila Bag-of-marbles protein in
... detected both BamC and BamF protein (anti-Bam2.0). Cytoplasmic staining (BamC) is clearly visible in a cluster of 8 cystocytes (5 visible) towards the top of the micrograph (labeled no. 3) as well as fainter staining in a 2-cell cluster (no. 2) towards the bottom. Fusome-associated antigen appears a ...
... detected both BamC and BamF protein (anti-Bam2.0). Cytoplasmic staining (BamC) is clearly visible in a cluster of 8 cystocytes (5 visible) towards the top of the micrograph (labeled no. 3) as well as fainter staining in a 2-cell cluster (no. 2) towards the bottom. Fusome-associated antigen appears a ...
From filaments to function: The role of the plant actin cytoskeleton in
... trafficking, while actin-dependent, functions independently of microtubules. In a parallel study, Ribeiro et al. (2013) came to a similar conclusion, demonstrating that nucleoprotein trafficking is actin-dependent and microtubule-independent, while further showing that actin was not required for the a ...
... trafficking, while actin-dependent, functions independently of microtubules. In a parallel study, Ribeiro et al. (2013) came to a similar conclusion, demonstrating that nucleoprotein trafficking is actin-dependent and microtubule-independent, while further showing that actin was not required for the a ...
Focusing on unpolymerized actin.
... 1988; Morgan et al., 1993; Ohta et al., 1989). The phosphorylated form of ADF lacks the ability to bind monomeric actin, and to affect the rate and extent of actin assembly. The fraction of ADF in the phosphorylated form varies from 0.15 to 0.6 in different cells and tissues (Morgan et al., 1993). I ...
... 1988; Morgan et al., 1993; Ohta et al., 1989). The phosphorylated form of ADF lacks the ability to bind monomeric actin, and to affect the rate and extent of actin assembly. The fraction of ADF in the phosphorylated form varies from 0.15 to 0.6 in different cells and tissues (Morgan et al., 1993). I ...
the fine-tuning of the endomembrane system
... the infected cells become mature N2-fixing cells. The vacuoles of the infected cells contract at this stage of cell development. This contraction permits the expansion of symbiosomes. Trafficking of tonoplast-targeted proteins in infected cells is also altered, as shown by retargeting of the aquapor ...
... the infected cells become mature N2-fixing cells. The vacuoles of the infected cells contract at this stage of cell development. This contraction permits the expansion of symbiosomes. Trafficking of tonoplast-targeted proteins in infected cells is also altered, as shown by retargeting of the aquapor ...
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... aggressive dissociation methods required to prepare isolated cell suspensions often affect the function of membrane ion channels and receptors with deterministic roles in cellular integration. Alternatively, both clinical and animal studies are too complex to reveal the mechanisms underlying the out ...
... aggressive dissociation methods required to prepare isolated cell suspensions often affect the function of membrane ion channels and receptors with deterministic roles in cellular integration. Alternatively, both clinical and animal studies are too complex to reveal the mechanisms underlying the out ...
Cosuppression of Eukaryotic Release Factor 1-1
... produced that overexpress an endogenous eRF1-1 cDNA (GenBank accession no. H37361). The eRF1-1 overexpression lines predominantly produced phenotypically normal plants, and we refer to these transgenics as nonphenotype plants. However, a limited percentage of plants (3%–60%) across multiple transgen ...
... produced that overexpress an endogenous eRF1-1 cDNA (GenBank accession no. H37361). The eRF1-1 overexpression lines predominantly produced phenotypically normal plants, and we refer to these transgenics as nonphenotype plants. However, a limited percentage of plants (3%–60%) across multiple transgen ...
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... Formation of the spindle is a third event central to meiotic cell division. During the spermatocyte growth phase, microtubules form a diffuse cytoplasmic network; this network persists until the cells mature. Late in meiotic prophase the centrosomes separate and move towards the poles (Fig. 3A, arro ...
... Formation of the spindle is a third event central to meiotic cell division. During the spermatocyte growth phase, microtubules form a diffuse cytoplasmic network; this network persists until the cells mature. Late in meiotic prophase the centrosomes separate and move towards the poles (Fig. 3A, arro ...
The pelota locus encodes a protein required for meiotic cell division
... Formation of the spindle is a third event central to meiotic cell division. During the spermatocyte growth phase, microtubules form a diffuse cytoplasmic network; this network persists until the cells mature. Late in meiotic prophase the centrosomes separate and move towards the poles (Fig. 3A, arro ...
... Formation of the spindle is a third event central to meiotic cell division. During the spermatocyte growth phase, microtubules form a diffuse cytoplasmic network; this network persists until the cells mature. Late in meiotic prophase the centrosomes separate and move towards the poles (Fig. 3A, arro ...
Nucleolus: the fascinating nuclear body
... appears necessary to generate a morphologically deWned nucleolus with its three distinct components, thereby maintaining the nucleolus in its usual organization. This suggests that such an organization results from the activity of ribosome biogenesis. Indeed nucleolar reorganization is induced when ...
... appears necessary to generate a morphologically deWned nucleolus with its three distinct components, thereby maintaining the nucleolus in its usual organization. This suggests that such an organization results from the activity of ribosome biogenesis. Indeed nucleolar reorganization is induced when ...
Early Morphogenesis of the Caenorhabditis elegans Pharynx
... morphogenesis to produce the characteristic bilobed structure of the mature pharynx. Because cell division is largely complete at this stage, pharyngeal extension is driven by forces other than cell proliferation (Sulston et al., 1983). We have used a combination of Nomarski differential interferenc ...
... morphogenesis to produce the characteristic bilobed structure of the mature pharynx. Because cell division is largely complete at this stage, pharyngeal extension is driven by forces other than cell proliferation (Sulston et al., 1983). We have used a combination of Nomarski differential interferenc ...
Sphingolipids Containing Very-Long
... attributed to depletion of VLCFA in sphingolipids. These mutants include cer10 (Zheng et al., 2005), gurke/pasticcino3 (pas3; Baud et al., 2004), pas2 (Bach et al., 2008), and pas1 (Roudier et al., 2010). In all these mutants, the level of VLCFA in sphingolipids is reduced, and this reduction is cou ...
... attributed to depletion of VLCFA in sphingolipids. These mutants include cer10 (Zheng et al., 2005), gurke/pasticcino3 (pas3; Baud et al., 2004), pas2 (Bach et al., 2008), and pas1 (Roudier et al., 2010). In all these mutants, the level of VLCFA in sphingolipids is reduced, and this reduction is cou ...
Novel role for the Lu/BCAM–spectrin interaction in actin cytoskeleton
... of specialized membrane domains and in vesicle trafficking. Spectrins exist as elongated flexible heterotetramers of 200 nm made up of two α- and β-subunits that constitute the filaments of the network, the nodes of which are cross-linked by actin filaments. In mammals, two genes encode for α-chains ...
... of specialized membrane domains and in vesicle trafficking. Spectrins exist as elongated flexible heterotetramers of 200 nm made up of two α- and β-subunits that constitute the filaments of the network, the nodes of which are cross-linked by actin filaments. In mammals, two genes encode for α-chains ...
What is new in tympanoplasty? - Romanian Journal of Rhinology
... The number of studies is growing rapidly: at the recent 10th International Conference on Cholesteatoma and Ear Surgery (5-8 June), Edinburgh, UK, a new study was reported by John J. Rosowski. The research group of Wyss Institute, Harvard University and Massachusetts Eye and Ear Infirmary has been ab ...
... The number of studies is growing rapidly: at the recent 10th International Conference on Cholesteatoma and Ear Surgery (5-8 June), Edinburgh, UK, a new study was reported by John J. Rosowski. The research group of Wyss Institute, Harvard University and Massachusetts Eye and Ear Infirmary has been ab ...
Observations of green fluorescent protein as a fusion partner in
... Cell growth was monitored by optical density (at 600 nm, OD600) on a UV/vis spectrophotometer (DU640, Beckman, Fullerton, CA). The total protein assay was performed using a protein assay kit (Bio-Rad Lab., Hercules, CA) with bovine serum albumin as a standard. The GFPuv whole cell assay was performe ...
... Cell growth was monitored by optical density (at 600 nm, OD600) on a UV/vis spectrophotometer (DU640, Beckman, Fullerton, CA). The total protein assay was performed using a protein assay kit (Bio-Rad Lab., Hercules, CA) with bovine serum albumin as a standard. The GFPuv whole cell assay was performe ...
The Amino-terminal Domain of the Golgi Protein Giantin Interacts
... FIG. 2. Association of full-length Giantin and Giantin fragments with p115. Panel A, coupled in vitro transcription/translation using [35S]methionine as the label was performed of plasmids encoding full-length Giantin (lanes 1–3), full-length Giantin lacking the NH2-terminal 449 residues (Gtn450 –31 ...
... FIG. 2. Association of full-length Giantin and Giantin fragments with p115. Panel A, coupled in vitro transcription/translation using [35S]methionine as the label was performed of plasmids encoding full-length Giantin (lanes 1–3), full-length Giantin lacking the NH2-terminal 449 residues (Gtn450 –31 ...
Uptake of glutamate, not glutamine synthetase, regulates adaptation
... of cell extract containing between 25 µg and 100 µg protein was incubated with 2.5 mM L-[U- 14C]glutamic acid (0.68 Ci/mol), 7.5 mM ATP, 30 mM MgCl2, 25 mM NH4Cl and 50 mM imidazoleHCl buffer (pH 7.5) in a final volume of 250 µl. After incubation for 60 min at 37oC the reaction was terminated by add ...
... of cell extract containing between 25 µg and 100 µg protein was incubated with 2.5 mM L-[U- 14C]glutamic acid (0.68 Ci/mol), 7.5 mM ATP, 30 mM MgCl2, 25 mM NH4Cl and 50 mM imidazoleHCl buffer (pH 7.5) in a final volume of 250 µl. After incubation for 60 min at 37oC the reaction was terminated by add ...
Heat Shock Responses for Understanding Diseases of
... and killing foreign microorganisms in phagocytic cells, have been extensively studied. The ROS defense system requires the NADPH oxidase complex (Nox), which generates H2O2 within the phagosome. Invasion by microorganisms leads to assembly of an active Nox complex, which comprises a catalytic subuni ...
... and killing foreign microorganisms in phagocytic cells, have been extensively studied. The ROS defense system requires the NADPH oxidase complex (Nox), which generates H2O2 within the phagosome. Invasion by microorganisms leads to assembly of an active Nox complex, which comprises a catalytic subuni ...
The FluidMosaic Model of the Structure of Cell Membranes
... possibility that the cooperative unit inThe phospholipids of membranes. volved in the phase transition is quite There is now substantial evidence that -small, consisting perhaps of only 100. the major portion of the phospholipids lipid molecules on the average. (ii) None is in bilayer form in a vari ...
... possibility that the cooperative unit inThe phospholipids of membranes. volved in the phase transition is quite There is now substantial evidence that -small, consisting perhaps of only 100. the major portion of the phospholipids lipid molecules on the average. (ii) None is in bilayer form in a vari ...
- Columbia University Medical Center
... transfection of plasmids into COS cells. Cells were harvested and media collected after 48 hr, separated by SDS-polyacrylamide gel electrophoresis (SDS-PAGE), transferred to nitrocellulose, and probed with antibodies direct against the amino-terminal (anti-NH2) (lanes 1-9) or carboxy-terminal (anti- ...
... transfection of plasmids into COS cells. Cells were harvested and media collected after 48 hr, separated by SDS-polyacrylamide gel electrophoresis (SDS-PAGE), transferred to nitrocellulose, and probed with antibodies direct against the amino-terminal (anti-NH2) (lanes 1-9) or carboxy-terminal (anti- ...
Slide 1
... and causes the insertion of several C9 molecules. bind to this complex and lead to formation of a hole in the membrane resulting in cell lysis. • The lysis of target cell by C5b6789 complex is nonenzymatic and is believed to be due to a physical change in the ...
... and causes the insertion of several C9 molecules. bind to this complex and lead to formation of a hole in the membrane resulting in cell lysis. • The lysis of target cell by C5b6789 complex is nonenzymatic and is believed to be due to a physical change in the ...
Delivery of a Secreted Soluble Protein to the Vacuole via a
... homogenization of the leaves. We prepared extracellular fluid from leaf tissue samples according to the method of Klement (1965), but found that it contained no yeast invertase (data not shown), whereas isolated protoplasts contained abundant amounts of soluble yeast invertase. To determine if the s ...
... homogenization of the leaves. We prepared extracellular fluid from leaf tissue samples according to the method of Klement (1965), but found that it contained no yeast invertase (data not shown), whereas isolated protoplasts contained abundant amounts of soluble yeast invertase. To determine if the s ...
Title Non-coding functions of alternative pre-mRNA - DR-NTU
... cells and required for proper cardiovascular development [35]. This exon interferes with DLG1 translation at least in part by introducing a short uORF into the 5’UTR sequence. Intron 1 in the 5’UTR of mRNA encoding chick proinsulin, an insulin precursor essential for proper development and metabolis ...
... cells and required for proper cardiovascular development [35]. This exon interferes with DLG1 translation at least in part by introducing a short uORF into the 5’UTR sequence. Intron 1 in the 5’UTR of mRNA encoding chick proinsulin, an insulin precursor essential for proper development and metabolis ...
Modeling the Gastric Mill Central Pattern Generator of the Lobster
... circles represent multiple cells that are regarded as identical for modeling purposes. All but the 2 E cells are in the stomatogastric ganglion (STG). The key to the types of connections in the network appears below the diagram. A functional inhibitory or excitatory connection means that not all the ...
... circles represent multiple cells that are regarded as identical for modeling purposes. All but the 2 E cells are in the stomatogastric ganglion (STG). The key to the types of connections in the network appears below the diagram. A functional inhibitory or excitatory connection means that not all the ...
The YidC/Oxa1/Alb3 protein family Saller, Manfred J.
... For some YidC homologs, the core structure seems to deviate from the general consensus. For example, the core of Synechocystis sp. PCC6803 (Slr1471) is predicted to contain a large periplasmatic domain between core TMS2 and TMS3 that is conserved within cyanobacteria and is essential for activity. T ...
... For some YidC homologs, the core structure seems to deviate from the general consensus. For example, the core of Synechocystis sp. PCC6803 (Slr1471) is predicted to contain a large periplasmatic domain between core TMS2 and TMS3 that is conserved within cyanobacteria and is essential for activity. T ...
Calcium Signaling through Protein Kinases. The Arabidopsis
... Based upon sequence homology, the CDPKs of Arabidopsis cluster into four subgroups (I–IV). Subgroup IV is the least complex, with three members, and subgroup II is the most complex, with 13 members. This pattern of grouping was also found when the tree was constructed based on the sequences of the k ...
... Based upon sequence homology, the CDPKs of Arabidopsis cluster into four subgroups (I–IV). Subgroup IV is the least complex, with three members, and subgroup II is the most complex, with 13 members. This pattern of grouping was also found when the tree was constructed based on the sequences of the k ...
Cytokinesis
Cytokinesis (cyto- + kinesis) is the process during cell division in which the cytoplasm of a single eukaryotic cell is divided to form two daughter cells. It usually initiates during the early stages of mitosis, and sometimes meiosis, splitting a mitotic cell in two, to ensure that chromosome number is maintained from one generation to the next. After cytokinesis two (daughter) cells will be formed that are exact copies of the (parent) original cell. After cytokinesis, each daughter cell is in the interphase portion of the cell cycle. In animal cells, one notable exception to the normal process of cytokinesis is oogenesis (the creation of an ovum in the ovarian follicle of the ovary), where the ovum takes almost all the cytoplasm and organelles, leaving very little for the resulting polar bodies, which then die. Another form of mitosis without cytokinesis occurs in the liver, yielding multinucleate cells. In plant cells, a dividing structure known as the cell plate forms within the centre of the cytoplasm and a new cell wall forms between the two daughter cells.Cytokinesis is distinguished from the prokaryotic process of binary fission.