Journal of Cell Biology - Institute of Cancer Research Repository
... linker between actin filaments and transmembrane proteins such as CD44, thereby providing an anchoring point in the plasma membrane for actin-myosin fibers. ROCK-induced contraction of NIH 3T3 cells was reported to be dependent on phosphorylation of Ezrin Thr567 (Tran Quang et al., 2000), which allo ...
... linker between actin filaments and transmembrane proteins such as CD44, thereby providing an anchoring point in the plasma membrane for actin-myosin fibers. ROCK-induced contraction of NIH 3T3 cells was reported to be dependent on phosphorylation of Ezrin Thr567 (Tran Quang et al., 2000), which allo ...
Platelet releasate increases the proliferation and
... Abstract: Concentrated platelets and their products are currently being used as a clinical tool to accelerate endosseous wound healing. However, there is little understanding regarding the actions of platelets and platelet-released products on osteogenic cells. We show, herein, that releasate from t ...
... Abstract: Concentrated platelets and their products are currently being used as a clinical tool to accelerate endosseous wound healing. However, there is little understanding regarding the actions of platelets and platelet-released products on osteogenic cells. We show, herein, that releasate from t ...
The World of Cells Kinds of Cells Tour of a Eukaryotic Cell Transport
... use of stains that bind to specific molecular targets. This approach has been used in the analysis of tissue samples, or histology, for many years and has been improved dramatically with the use of antibiotics that bind to very specific molecular structures. This process, called immunocytochemistry, u ...
... use of stains that bind to specific molecular targets. This approach has been used in the analysis of tissue samples, or histology, for many years and has been improved dramatically with the use of antibiotics that bind to very specific molecular structures. This process, called immunocytochemistry, u ...
When Cells Become Organelle Donors
... mice, mesenchymal stem cells injected into the trachea successfully transfer mitochondria to alveolar epithelial cells of the lung when it is injured with endotoxin but not when it is uninjured (12). When injected into the healthy lung, the mesenchymal stem cells quickly migrate to the interstitium ...
... mice, mesenchymal stem cells injected into the trachea successfully transfer mitochondria to alveolar epithelial cells of the lung when it is injured with endotoxin but not when it is uninjured (12). When injected into the healthy lung, the mesenchymal stem cells quickly migrate to the interstitium ...
Regulation of ion channels
... • Local depolarization of membrane spreads to adjacent region (purple) opening voltage-gated Na+ channels • This causes further depolarization of membrane and opening of additional voltage-gated Na+ channels • Na+ channels in the region of the membrane (green) where the action potential originated r ...
... • Local depolarization of membrane spreads to adjacent region (purple) opening voltage-gated Na+ channels • This causes further depolarization of membrane and opening of additional voltage-gated Na+ channels • Na+ channels in the region of the membrane (green) where the action potential originated r ...
Mechanisms of Hormone Action
... other proteins. Insulin is an example of a hormone whose receptor is a tyrosine kinase. The hormone binds to domains exposed on the cell's surface, resulting in a conformational change that activates kinase domains located in the cytoplasmic regions of the receptor. In many cases, the receptor phosp ...
... other proteins. Insulin is an example of a hormone whose receptor is a tyrosine kinase. The hormone binds to domains exposed on the cell's surface, resulting in a conformational change that activates kinase domains located in the cytoplasmic regions of the receptor. In many cases, the receptor phosp ...
Gamete Handling and Fertilization
... sea urchin embryos, but its effects on fertilization have remained unexplored. To address this, sea urchin eggs (Arabacia punculata) were incubated for 10 minutes in varying concentrations of Lithium Chloride (LiCl); 0.01M, 0.1M, 0.3M, and 0.5M. After the incubation period, fertilization was attempt ...
... sea urchin embryos, but its effects on fertilization have remained unexplored. To address this, sea urchin eggs (Arabacia punculata) were incubated for 10 minutes in varying concentrations of Lithium Chloride (LiCl); 0.01M, 0.1M, 0.3M, and 0.5M. After the incubation period, fertilization was attempt ...
Cajal bodies and coilin—moving towards function
... CBs can separate into two daughter bodies and join to form larger bodies. CB-joining events appear to be common and can involve CBs translocating large distances through the nucleoplasm. Separation of CBs into smaller structures can result in the asymmetric segregation of resident CB proteins betwee ...
... CBs can separate into two daughter bodies and join to form larger bodies. CB-joining events appear to be common and can involve CBs translocating large distances through the nucleoplasm. Separation of CBs into smaller structures can result in the asymmetric segregation of resident CB proteins betwee ...
Biology: Concepts and Connections, 6e (Campbell)
... A) have hydrophobic heads that face the center of the membrane and are shielded from water. B) have hydrophilic tails that face outward and are exposed to water. C) often have "kinks" in their tails caused by the presence of a double bond between carbons. D) remain fluid because they are tightly pac ...
... A) have hydrophobic heads that face the center of the membrane and are shielded from water. B) have hydrophilic tails that face outward and are exposed to water. C) often have "kinks" in their tails caused by the presence of a double bond between carbons. D) remain fluid because they are tightly pac ...
Investigating the organization, assembly and physical properties of
... School of Physics & Astronomy, University of Leeds Astbury Centre for Structural Molecular Biology, University of Leeds Biological cells and some internal structures are surrounded by membranes comprised of lipid bilayers and membrane proteins. Certain specialized biomembranes are stacked into multi ...
... School of Physics & Astronomy, University of Leeds Astbury Centre for Structural Molecular Biology, University of Leeds Biological cells and some internal structures are surrounded by membranes comprised of lipid bilayers and membrane proteins. Certain specialized biomembranes are stacked into multi ...
The Plastidial 2-C-Methyl-D-Erythritol 4-Phosphate
... solely responsible for the biosynthesis of the isoprenyl diphosphates used by plant cells for protein isoprenylation. Indeed, incubation of BY-2 cells in the presence of [2-14C]1-deoxy-Dxylulose (DX), the dephosphorylated form of the first product of the MEP pathway, resulted in significant labeling ...
... solely responsible for the biosynthesis of the isoprenyl diphosphates used by plant cells for protein isoprenylation. Indeed, incubation of BY-2 cells in the presence of [2-14C]1-deoxy-Dxylulose (DX), the dephosphorylated form of the first product of the MEP pathway, resulted in significant labeling ...
DLG5 in Cell Polarity Maintenance and Cancer Development
... PDZ3-PDZ4 and SH3-GUK domains of DLG5 also have self-association potential and can bind to the N-terminus of DL5 [22]. The characteristics of the coiled coil, PDZ3-PDZ4 and SH3-GUK domains suggest that DLG5 can self-associate and form a large protein complex containing multiple β-cateninbinding doma ...
... PDZ3-PDZ4 and SH3-GUK domains of DLG5 also have self-association potential and can bind to the N-terminus of DL5 [22]. The characteristics of the coiled coil, PDZ3-PDZ4 and SH3-GUK domains suggest that DLG5 can self-associate and form a large protein complex containing multiple β-cateninbinding doma ...
Actin Microfilaments Regulate Vacuolar Structures and Dynamics
... Fig. 2 Localization of MFs and VMs in living BY-2 cells. (A–C) A BY-GF11 cell was observed by CLSM in bright field (A), and by GFP– fimbrin (B) and FM4-64 (C). Cortical MFs (small arrows) and the MFs close to the VM (large arrows) were observed. In the cytoplasmic strands, the MFs were localized clo ...
... Fig. 2 Localization of MFs and VMs in living BY-2 cells. (A–C) A BY-GF11 cell was observed by CLSM in bright field (A), and by GFP– fimbrin (B) and FM4-64 (C). Cortical MFs (small arrows) and the MFs close to the VM (large arrows) were observed. In the cytoplasmic strands, the MFs were localized clo ...
chapter3_part1 Membrane lecture
... • The rate of endocytosis and exocytosis must be balanced to maintain a constant membrane surface area and cell volume • Caveolae may play a role in transport of substances and cell signaling ...
... • The rate of endocytosis and exocytosis must be balanced to maintain a constant membrane surface area and cell volume • Caveolae may play a role in transport of substances and cell signaling ...
Tissue Engineering - Gateway Coalition
... minimize the use of animals, using animal models only when necessary and at a later stage of material development. ...
... minimize the use of animals, using animal models only when necessary and at a later stage of material development. ...
Characterization of Dependencies Between Growth and
... These time-lapse datasets also allow investigation of correlations between measurements made at different cell cycles, an important gap in our understanding of coordination between growth and division. In multicellular systems, coordination of division among cells has important implications for high ...
... These time-lapse datasets also allow investigation of correlations between measurements made at different cell cycles, an important gap in our understanding of coordination between growth and division. In multicellular systems, coordination of division among cells has important implications for high ...
Isolation of N-Acetylmuramoyl-L-Alanine Amidase Gene (amiB) from
... Compared with the wild-type strain, the mutant strain also showed susceptibility to growth inhibition and lysis by toxic molecules [8]. In this study, we compared the survival rate of the amiB deletion mutant constructed by the allelic exchange method in the presence of organic acids. When acetate a ...
... Compared with the wild-type strain, the mutant strain also showed susceptibility to growth inhibition and lysis by toxic molecules [8]. In this study, we compared the survival rate of the amiB deletion mutant constructed by the allelic exchange method in the presence of organic acids. When acetate a ...
Marxreiter EMBO2014 - Carl Thummel`s
... well as the Pdf peptide hormone, which is related to mammalian vasoactive intestinal polypeptide (Fig 1). Genetic inactivation of these neurons resulted in reduced posterior gut tracheation, while increasing their neuronal activity resulted in increased terminal cell branching in this region, indica ...
... well as the Pdf peptide hormone, which is related to mammalian vasoactive intestinal polypeptide (Fig 1). Genetic inactivation of these neurons resulted in reduced posterior gut tracheation, while increasing their neuronal activity resulted in increased terminal cell branching in this region, indica ...
New insights into the evolutionary history of photoreceptor cells
... Metazoan photoreceptors can be divided into ciliary and rhabdomeric classes. Ciliary photoreceptors are commonly associated with vertebrates, whereas rhabdomeric photoreceptors are usually considered to be an invertebrate characteristic. Both cell types are neurons that are specialized for photorece ...
... Metazoan photoreceptors can be divided into ciliary and rhabdomeric classes. Ciliary photoreceptors are commonly associated with vertebrates, whereas rhabdomeric photoreceptors are usually considered to be an invertebrate characteristic. Both cell types are neurons that are specialized for photorece ...
Exine dehiscing induces rape microspore polarity
... zygotic division (Haecker et al., 2004), suggesting that the two daughter cells may possess different transcripts from the zygote. After zygote division, WOX8 is expressed in the basal cell lineage (Minako et al., 2011), and WOX8 can be used as a marker for the basal cell lineage (Haecker et al., ...
... zygotic division (Haecker et al., 2004), suggesting that the two daughter cells may possess different transcripts from the zygote. After zygote division, WOX8 is expressed in the basal cell lineage (Minako et al., 2011), and WOX8 can be used as a marker for the basal cell lineage (Haecker et al., ...
Insulin-Resistance, Browning
... relatively scarce in the adult as an identifiable tissue. Brown fat cells are interspersed within WAT of rodents and humans. Activation of BAT requires 3-adrenergic receptor agonism. ...
... relatively scarce in the adult as an identifiable tissue. Brown fat cells are interspersed within WAT of rodents and humans. Activation of BAT requires 3-adrenergic receptor agonism. ...
I can - Coldwater Community Schools
... ATP--How do cells get energy from phosphates? What is the energy used for? Know the reaction on how to make and break ATP. ...
... ATP--How do cells get energy from phosphates? What is the energy used for? Know the reaction on how to make and break ATP. ...
Direct interaction of two polarity complexes implicated in epithelial
... In summary, our results identify an unexpected direct interaction between PALS1 and Par6 that links two protein complexes important for epithelial cell polarity. Crb is localized to the apical surface of mammalian and fly epithelia and colocalizes at the tight junction with PALS1 and PATJ2–4,21. Thr ...
... In summary, our results identify an unexpected direct interaction between PALS1 and Par6 that links two protein complexes important for epithelial cell polarity. Crb is localized to the apical surface of mammalian and fly epithelia and colocalizes at the tight junction with PALS1 and PATJ2–4,21. Thr ...
Gram Stain
... components; the primary stain, a mordant that sets the stain, a decolorizing agent to remove the primary stain, and a counter stain. In the Gram stain, the primary stain is crystal violet. This gives the cell an intense purple color. The mordant, iodine, forms a complex with the crystal violet insid ...
... components; the primary stain, a mordant that sets the stain, a decolorizing agent to remove the primary stain, and a counter stain. In the Gram stain, the primary stain is crystal violet. This gives the cell an intense purple color. The mordant, iodine, forms a complex with the crystal violet insid ...
Cytokinesis
Cytokinesis (cyto- + kinesis) is the process during cell division in which the cytoplasm of a single eukaryotic cell is divided to form two daughter cells. It usually initiates during the early stages of mitosis, and sometimes meiosis, splitting a mitotic cell in two, to ensure that chromosome number is maintained from one generation to the next. After cytokinesis two (daughter) cells will be formed that are exact copies of the (parent) original cell. After cytokinesis, each daughter cell is in the interphase portion of the cell cycle. In animal cells, one notable exception to the normal process of cytokinesis is oogenesis (the creation of an ovum in the ovarian follicle of the ovary), where the ovum takes almost all the cytoplasm and organelles, leaving very little for the resulting polar bodies, which then die. Another form of mitosis without cytokinesis occurs in the liver, yielding multinucleate cells. In plant cells, a dividing structure known as the cell plate forms within the centre of the cytoplasm and a new cell wall forms between the two daughter cells.Cytokinesis is distinguished from the prokaryotic process of binary fission.