Fractionation of Membranes from Metschnikowi`a
... cell membrane homogenate and correlated with a high specific activity of cytochrome c oxidase and an ATPase with maximum activity at pH 8.5. These two enzyme markers were characteristic of mitochondria, designated M, (Fig. 3a, 6). A subsequent centrifugation of the two membrane fractions, the plasma ...
... cell membrane homogenate and correlated with a high specific activity of cytochrome c oxidase and an ATPase with maximum activity at pH 8.5. These two enzyme markers were characteristic of mitochondria, designated M, (Fig. 3a, 6). A subsequent centrifugation of the two membrane fractions, the plasma ...
supplementary information
... to reside within the cytoplasm1,2 and presumably hydrolyzes any sucrose that is directly imported by the non-specific AGT1 permease3. We find that an AGT1 knockout strain is able to grow on sucrose in dilute cellular conditions but with a slight growth defect (~15%, data not shown), suggesting that ...
... to reside within the cytoplasm1,2 and presumably hydrolyzes any sucrose that is directly imported by the non-specific AGT1 permease3. We find that an AGT1 knockout strain is able to grow on sucrose in dilute cellular conditions but with a slight growth defect (~15%, data not shown), suggesting that ...
New and Old Hereditary Forms of Renal Cell Carcinoma (RCC)
... Cysts are frequently present in VHL, with a multilocular growth pattern. They vary in sized and are separated by fibrous / hyalinized septa. The cysts are usually lined by a single row of low nuclear grade tumor cells. The VHL gene is known to be located in 3p25-p26, but changes have been reported i ...
... Cysts are frequently present in VHL, with a multilocular growth pattern. They vary in sized and are separated by fibrous / hyalinized septa. The cysts are usually lined by a single row of low nuclear grade tumor cells. The VHL gene is known to be located in 3p25-p26, but changes have been reported i ...
A Geminivirus lnduces Expression of a Host DNA Synthesis Protein
... In some cases, labeled cells were severely necrotic, as indicated by a uniform loss of chlorophyll autofluorescence throughout the cell (Figure 21). Cells showing different degrees of necrosis were seen in leaf tissue, as demonstrated by the necrotic cells in Figures 2C, 2H, and 21. Necrotic cells, ...
... In some cases, labeled cells were severely necrotic, as indicated by a uniform loss of chlorophyll autofluorescence throughout the cell (Figure 21). Cells showing different degrees of necrosis were seen in leaf tissue, as demonstrated by the necrotic cells in Figures 2C, 2H, and 21. Necrotic cells, ...
Vacuole Membrane Protein 1 Is an Endoplasmic
... One of the hallmarks of eukaryotic cells is the presence of complex intracellular membrane-bound organelles dedicated to specific functions. Part of the structural and functional specificity of these organelles is based on their distinct complement of proteins and membrane lipids. The link between m ...
... One of the hallmarks of eukaryotic cells is the presence of complex intracellular membrane-bound organelles dedicated to specific functions. Part of the structural and functional specificity of these organelles is based on their distinct complement of proteins and membrane lipids. The link between m ...
CELL DIVISION AND DNA SYNTHESIS IN TETRAHYMENA
... at which D N A synthesis begins is also demonstrated by the change in the utilization of H 3thymidine of the medium for macronuclear D N A synthesis. Amino acid deprivation before D N A synthesis begins prevents the subsequent uptake of H3-thymidine into macronuclear D N A although macronuclear D N ...
... at which D N A synthesis begins is also demonstrated by the change in the utilization of H 3thymidine of the medium for macronuclear D N A synthesis. Amino acid deprivation before D N A synthesis begins prevents the subsequent uptake of H3-thymidine into macronuclear D N A although macronuclear D N ...
Effect of Gibberellic Acid and Actinomycin D on the Formation and
... does not grant permission for this article to be further copied/distributed or hosted elsewhere without the express permission from American Society of Plant Biologists. ...
... does not grant permission for this article to be further copied/distributed or hosted elsewhere without the express permission from American Society of Plant Biologists. ...
Isolation, Characterization, and Expression of cDNAs Encoding
... Saccharomyces cerevisiae. Partial human a-mannosidase II cDNA clones were also isolated and the gene was localized to human chromosome 5. ...
... Saccharomyces cerevisiae. Partial human a-mannosidase II cDNA clones were also isolated and the gene was localized to human chromosome 5. ...
Phosphotyrosine dependent proteinprotein interaction network
... 2008; Koytiger et al, 2013; Tinti et al, 2013). Though amino acid residues surrounding the phospho-tyrosine clearly contribute to recognition specificity, their predictive value on a proteome scale for whether a tyrosine phosphorylation promotes an SH2- or PTBmediated protein interaction is relative ...
... 2008; Koytiger et al, 2013; Tinti et al, 2013). Though amino acid residues surrounding the phospho-tyrosine clearly contribute to recognition specificity, their predictive value on a proteome scale for whether a tyrosine phosphorylation promotes an SH2- or PTBmediated protein interaction is relative ...
Src1 is a Protein of the Inner Nuclear Membrane Interacting with the
... and proteins of the helix-extension-helix (HeH) superfamily of DNA-binding INM proteins [6]. Among the latter is a group of intensively-studied proteins known as LEM-domain proteins, named for a shared, conserved domain found in lamina-associated polypeptide 2 (LAP2), Emerin, and MAN1 [7]. In metazo ...
... and proteins of the helix-extension-helix (HeH) superfamily of DNA-binding INM proteins [6]. Among the latter is a group of intensively-studied proteins known as LEM-domain proteins, named for a shared, conserved domain found in lamina-associated polypeptide 2 (LAP2), Emerin, and MAN1 [7]. In metazo ...
Experimental taphonomy of giant sulphur bacteria: implications for
... Furthermore, reports of nucleus-like bodies in the fossils [15 –17] are seemingly at odds with a prokaryote hypothesis. In spite of these problems, giant sulphur bacteria offer a potential explanation of the Doushantuo fossils, whose kingdom-level identification remains uncertain. Moreover, giant su ...
... Furthermore, reports of nucleus-like bodies in the fossils [15 –17] are seemingly at odds with a prokaryote hypothesis. In spite of these problems, giant sulphur bacteria offer a potential explanation of the Doushantuo fossils, whose kingdom-level identification remains uncertain. Moreover, giant su ...
Parasitic fungi on roses - Formatex Research Center
... serious pre- and postharvest diseases in more than 200 plant species, including agriculturally important crops and harvested commodities, such as grapes, tomatoes, strawberries, cucumbers, bulb flowers, cut flowers and ornamental plants [24]. The broad host range of B. cinerea results in great econo ...
... serious pre- and postharvest diseases in more than 200 plant species, including agriculturally important crops and harvested commodities, such as grapes, tomatoes, strawberries, cucumbers, bulb flowers, cut flowers and ornamental plants [24]. The broad host range of B. cinerea results in great econo ...
Dynamic Localization of the DNA Replication
... replication fork progresses, the MCM complex is released into the nucleoplasm and must be prevented from reloading onto nascent DNA, as unauthorized loading can result in rereplication (Fujita et al., 1996; Tsuruga et al., 1997; Kearsey and Labib, 1998; Namdar and Kearsey, 2006). Distinct mechanisms ...
... replication fork progresses, the MCM complex is released into the nucleoplasm and must be prevented from reloading onto nascent DNA, as unauthorized loading can result in rereplication (Fujita et al., 1996; Tsuruga et al., 1997; Kearsey and Labib, 1998; Namdar and Kearsey, 2006). Distinct mechanisms ...
Molecular Mechanisms of Transforming Growth Factor
... Deregulation of TGF-b signaling is implicated in the pathogenesis of many diseases including arthritis, atherosclerosis, glomerulonephritis, human hereditary telangiectasia, and carcinogenesis. Loss of cellular sensitivity to TGFb-mediated growth inhibition may contribute directly to these pathologi ...
... Deregulation of TGF-b signaling is implicated in the pathogenesis of many diseases including arthritis, atherosclerosis, glomerulonephritis, human hereditary telangiectasia, and carcinogenesis. Loss of cellular sensitivity to TGFb-mediated growth inhibition may contribute directly to these pathologi ...
Signaling tip growth in plants Zhenbiao Yang
... tubes into bulbous tubes (H Li and Z Yang, unpublished data). These results indicate that Rop controls not only the establishment of cell polarity but also tip growth, as does CDC42 in tip-growing fission yeast [8]. The bulbous phenotype is analogous to tip swelling accompanied by non-localized intr ...
... tubes into bulbous tubes (H Li and Z Yang, unpublished data). These results indicate that Rop controls not only the establishment of cell polarity but also tip growth, as does CDC42 in tip-growing fission yeast [8]. The bulbous phenotype is analogous to tip swelling accompanied by non-localized intr ...
Mechanisms of cell rearrangement and cell
... shortly before puparium formation. Stacks emerge in a twoovaries were stained with phalloidin, which binds to actin dimensional pattern which is oriented along the medial-lateral filaments. Before cluster formation begins, TF cells and apical and dorsal-ventral axis. The first two stacks begin to fo ...
... shortly before puparium formation. Stacks emerge in a twoovaries were stained with phalloidin, which binds to actin dimensional pattern which is oriented along the medial-lateral filaments. Before cluster formation begins, TF cells and apical and dorsal-ventral axis. The first two stacks begin to fo ...
Profibrillin conversion by proprotein convertases
... terminus. Incubation with an R-R/K-R-X endoprotease created additional fragments for both proteins, indicating that the proprotein convertases recognized only the basic amino acid cleavage site in the carboxyl terminus (Fig. 3). Incubation of radiolabelled mini-profibrillin with dermal fibroblasts a ...
... terminus. Incubation with an R-R/K-R-X endoprotease created additional fragments for both proteins, indicating that the proprotein convertases recognized only the basic amino acid cleavage site in the carboxyl terminus (Fig. 3). Incubation of radiolabelled mini-profibrillin with dermal fibroblasts a ...
Establishment of Polarity during Organization of the Acentrosomal
... Monitoring Editor: J. Richard McIntosh ...
... Monitoring Editor: J. Richard McIntosh ...
The Balance between Cell Division and Endoreplication Depends
... depends on the coordination of gene expression, posttranslational modification, and specific proteolysis of cell cycle regulators. The G1/S and G2/M transitions are critical checkpoints controlled, in part, by cyclin-dependent kinases in the retinoblastoma (RBR)/E2F/DP pathway. Arabidopsis thaliana ...
... depends on the coordination of gene expression, posttranslational modification, and specific proteolysis of cell cycle regulators. The G1/S and G2/M transitions are critical checkpoints controlled, in part, by cyclin-dependent kinases in the retinoblastoma (RBR)/E2F/DP pathway. Arabidopsis thaliana ...
Is the Cytoskeleton Necessary for Viral Replication?
... lysis. When trafficking out, the viruses must make use of kinesin and/or myosin. The actin cortex can be a barrier to some viruses, which is why brief periods of actin depolymerization increase the release of some viruses (Radtke et al., 2006). The cell cycle is highly regulated by many proteins and ...
... lysis. When trafficking out, the viruses must make use of kinesin and/or myosin. The actin cortex can be a barrier to some viruses, which is why brief periods of actin depolymerization increase the release of some viruses (Radtke et al., 2006). The cell cycle is highly regulated by many proteins and ...
Induction of S phase stasis - Journal of Cell Science
... of control to ensure that replication of nuclear DNA occurs once and only once during a cell cycle. Evidence from cell fusion experiments (Rao and Johnson, 1970) and from in vitro replication assays of isolated nuclei (Krude et al., 1997) has demonstrated that G1 nuclei are competent to engage in re ...
... of control to ensure that replication of nuclear DNA occurs once and only once during a cell cycle. Evidence from cell fusion experiments (Rao and Johnson, 1970) and from in vitro replication assays of isolated nuclei (Krude et al., 1997) has demonstrated that G1 nuclei are competent to engage in re ...
Myosin binding proteins - Journal of Cell Science
... myosin is polymerized in vitro at physiological stoichiometries with MyBP-C, the resulting thick filaments are slightly longer and more regular than in the absence of MyBP-C (Koretz, 1979; Davis, 1988). The presence of MyBP-C reduces the critical concentration required for myosin polymerization sugg ...
... myosin is polymerized in vitro at physiological stoichiometries with MyBP-C, the resulting thick filaments are slightly longer and more regular than in the absence of MyBP-C (Koretz, 1979; Davis, 1988). The presence of MyBP-C reduces the critical concentration required for myosin polymerization sugg ...
Phloem Tubes
... concentration (30%) found in companion cells and sieve tube elements Thus, sucrose moves against a concentration to get into the sieve tube elements Movement occurs by active transport active loading of amino acids, sucrose, phosphates, potassium and ammonium ions into the companion cells are ...
... concentration (30%) found in companion cells and sieve tube elements Thus, sucrose moves against a concentration to get into the sieve tube elements Movement occurs by active transport active loading of amino acids, sucrose, phosphates, potassium and ammonium ions into the companion cells are ...
the single-cell preparation guide
... staining, and quality control that are essential to the preparation of high-quality single-cell suspensions for use with the C1™ system. The guide begins with workflows that are organized by sample. See the Sample Preparation Workflow below. Click a step in the appropriate workflow to find a protoco ...
... staining, and quality control that are essential to the preparation of high-quality single-cell suspensions for use with the C1™ system. The guide begins with workflows that are organized by sample. See the Sample Preparation Workflow below. Click a step in the appropriate workflow to find a protoco ...
Endocytosis Via Caveolae
... more constricted at the mouth, or they may contain a diaphragm that restricts diffusion (15,16). In muscle cells, caveolae are often observed in the form of composite clusters or linear rows of multiple flask-shaped units involved in the formation of T-tubules (17,18). In epithelial tissue culture c ...
... more constricted at the mouth, or they may contain a diaphragm that restricts diffusion (15,16). In muscle cells, caveolae are often observed in the form of composite clusters or linear rows of multiple flask-shaped units involved in the formation of T-tubules (17,18). In epithelial tissue culture c ...
Cytokinesis
Cytokinesis (cyto- + kinesis) is the process during cell division in which the cytoplasm of a single eukaryotic cell is divided to form two daughter cells. It usually initiates during the early stages of mitosis, and sometimes meiosis, splitting a mitotic cell in two, to ensure that chromosome number is maintained from one generation to the next. After cytokinesis two (daughter) cells will be formed that are exact copies of the (parent) original cell. After cytokinesis, each daughter cell is in the interphase portion of the cell cycle. In animal cells, one notable exception to the normal process of cytokinesis is oogenesis (the creation of an ovum in the ovarian follicle of the ovary), where the ovum takes almost all the cytoplasm and organelles, leaving very little for the resulting polar bodies, which then die. Another form of mitosis without cytokinesis occurs in the liver, yielding multinucleate cells. In plant cells, a dividing structure known as the cell plate forms within the centre of the cytoplasm and a new cell wall forms between the two daughter cells.Cytokinesis is distinguished from the prokaryotic process of binary fission.