Figure 8-9
... cations so both Na+ and K+ are permeable? (e.g., the Ach muscarinic receptor in the motor end plate of skeletal muscle.) • If ACh were to open an ion channel permeable only to K+, then the reversal potential of the end plate would be at the equilibrium potential for K+, which for a muscle cell is cl ...
... cations so both Na+ and K+ are permeable? (e.g., the Ach muscarinic receptor in the motor end plate of skeletal muscle.) • If ACh were to open an ion channel permeable only to K+, then the reversal potential of the end plate would be at the equilibrium potential for K+, which for a muscle cell is cl ...
Single-Cell Isolation and Gene Analysis: Pitfalls and
... several additional lasers may be used including 375 nm (>7 mW), 405 nm (>50 mW), and 561 nm (>18 mW). The fast flowing liquid allows for single-cell separating before passing between one or several lasers and a detector. As individual cells pass, the detector measures light scatter from the emitting ...
... several additional lasers may be used including 375 nm (>7 mW), 405 nm (>50 mW), and 561 nm (>18 mW). The fast flowing liquid allows for single-cell separating before passing between one or several lasers and a detector. As individual cells pass, the detector measures light scatter from the emitting ...
Isolation and Characterization of Conditional-Lethal Mutations in the TUB1 alpha-Tubulin Gene of the Yeast Saccharomyces cerevisiae .
... demonstrated the role of microtubules in chromothat are components of yeast microtubules (SCHATZet some separation on spindles and in nuclear movemental. 1986). T h e functional differences between these during mitoticgrowthandmating (DELGADOand two genes have been examined through the construcCONDE ...
... demonstrated the role of microtubules in chromothat are components of yeast microtubules (SCHATZet some separation on spindles and in nuclear movemental. 1986). T h e functional differences between these during mitoticgrowthandmating (DELGADOand two genes have been examined through the construcCONDE ...
07 Interneuronal connections
... 4. Ca++ removed from synaptic knob by mitochondria or calcium-pumps. 5. NT diffuses across synaptic cleft and binds to receptor on postsynaptic membran 6. Receptor changes shape of ion channel opening it and changing membrane potential 7. NT is quickly destroyed by enzymes or taken back up by astroc ...
... 4. Ca++ removed from synaptic knob by mitochondria or calcium-pumps. 5. NT diffuses across synaptic cleft and binds to receptor on postsynaptic membran 6. Receptor changes shape of ion channel opening it and changing membrane potential 7. NT is quickly destroyed by enzymes or taken back up by astroc ...
Chapter ONE - VU Research Portal
... Soni et al., 2008). Thus cyclin A2 appears required to initiate the first mitotic events. The localization of cyclin A is restricted to the nucleus, whereas cyclin B1 is cytoplasmic in G2 phase (Figure 3). The activation of cyclin B1-Cdk1 initially takes place on centrosomes, the organizing centers ...
... Soni et al., 2008). Thus cyclin A2 appears required to initiate the first mitotic events. The localization of cyclin A is restricted to the nucleus, whereas cyclin B1 is cytoplasmic in G2 phase (Figure 3). The activation of cyclin B1-Cdk1 initially takes place on centrosomes, the organizing centers ...
Efficient Endosomal Localization of Major Histocompatibility
... the cell lines were immunoprecipitated with 10.2-16, P4H5, or In-1 (Fig. 2). In all cases, anti-class II coprecipitates the relevant Ii molecules expressed by the cell lines. Furthermore, the appearance of c~, [3, and Ii in an approximately equal molar ratio indicates that the majority of class II i ...
... the cell lines were immunoprecipitated with 10.2-16, P4H5, or In-1 (Fig. 2). In all cases, anti-class II coprecipitates the relevant Ii molecules expressed by the cell lines. Furthermore, the appearance of c~, [3, and Ii in an approximately equal molar ratio indicates that the majority of class II i ...
The yeast integral membrane protein Apq12 potentially links
... (Fig. 2). We also examined the localization of Nup159/Rat7 and Pom152 by indirect immunofluorescence (IF) using antibodies directed against each protein. Nuclear basket components Nup1 and Nup60 were not mislocalized in apq12∆ cells nor were Sac3 or Mlp1, two proteins that associate with nuclear bas ...
... (Fig. 2). We also examined the localization of Nup159/Rat7 and Pom152 by indirect immunofluorescence (IF) using antibodies directed against each protein. Nuclear basket components Nup1 and Nup60 were not mislocalized in apq12∆ cells nor were Sac3 or Mlp1, two proteins that associate with nuclear bas ...
Role of the p53 Tumor Suppressor Gene in Cell Cycle Arrest and
... p53 gene in 88% of the cases analyzed (15 correct of 17 tested). This approach therefore provides a simple means to assess the functional status of p53 in cultured cell lines. We found that the presence of Epstein-Barr virus in several of the wild type p53 lines tested did not significantly alter th ...
... p53 gene in 88% of the cases analyzed (15 correct of 17 tested). This approach therefore provides a simple means to assess the functional status of p53 in cultured cell lines. We found that the presence of Epstein-Barr virus in several of the wild type p53 lines tested did not significantly alter th ...
Biogenesis of photosynthetic complexes in the chloroplast of
... presence of a single transmembrane (TM) segment, typically of~20 amino acids, very close to the C-terminus, at no more than 30 residues. This TM segment provides both the targeting signal for the delivery of the protein to the correct subcellular compartment and the anchor that retains the polypepti ...
... presence of a single transmembrane (TM) segment, typically of~20 amino acids, very close to the C-terminus, at no more than 30 residues. This TM segment provides both the targeting signal for the delivery of the protein to the correct subcellular compartment and the anchor that retains the polypepti ...
The UDPase activity of the Kluyveromyces lactis Golgi GDPase has
... As can be seen in Figure 3, transport of UDP-GlcNAc into the lumen of Golgi-enriched vesicles from K. lactis Klgda1 null mutants was approximately half compared to that into wildtype vesicles. This reduction was seen in both solutes within vesicles as well as in sugars bound to macromolecules within ...
... As can be seen in Figure 3, transport of UDP-GlcNAc into the lumen of Golgi-enriched vesicles from K. lactis Klgda1 null mutants was approximately half compared to that into wildtype vesicles. This reduction was seen in both solutes within vesicles as well as in sugars bound to macromolecules within ...
The perichromatin region of the plant cell nucleus is the area with
... Barta 2008). Similar to animal CBs, plant CBs participate in the transcription and processing of snRNAs (Schul et al. 1998; Boundonck et al. 1999; Darzacq et al. 2002). Recently, only CB functions that are specific to plant cells have been identified. For example, in plant cells, CBs participate in ...
... Barta 2008). Similar to animal CBs, plant CBs participate in the transcription and processing of snRNAs (Schul et al. 1998; Boundonck et al. 1999; Darzacq et al. 2002). Recently, only CB functions that are specific to plant cells have been identified. For example, in plant cells, CBs participate in ...
REGULATION OF INITIATION OF DNA SYNTHESIS
... placed in F-10 medium supplemented with dialyzed calf serum but singly deficient in either isoleucine or glutamine . Then different quantities of isoleucine were added back to the cultures deficient in isoleucine . Similarly, cultures were set up in F-10 medium containing varying quantities of gluta ...
... placed in F-10 medium supplemented with dialyzed calf serum but singly deficient in either isoleucine or glutamine . Then different quantities of isoleucine were added back to the cultures deficient in isoleucine . Similarly, cultures were set up in F-10 medium containing varying quantities of gluta ...
glycolytic and associated enzymes of rainbow trout (oncorhynchus
... Although the membrane ion exchange processes involved in adrenoceptormediated pH regulation by salmonid red cells are well known (Nikinmaa and Tufts, 1989; Motais and Garcia-Romeu, 1989), less is known about the nature or the response of the cellular metabolic pathways. The mature nucleated red cell ...
... Although the membrane ion exchange processes involved in adrenoceptormediated pH regulation by salmonid red cells are well known (Nikinmaa and Tufts, 1989; Motais and Garcia-Romeu, 1989), less is known about the nature or the response of the cellular metabolic pathways. The mature nucleated red cell ...
Cytostatic factor: an activity that puts the cell cycle on hold
... if one assumes that Emi1 behaves differently in meiosis and mitosis. However, the function of Emi1 as a CSF component was further called into question when Kishimoto and colleagues reported that endogenous Emi1 is undetectable in CSF-arrested Xenopus oocytes (Ohsumi et al., 2004). Furthermore, these ...
... if one assumes that Emi1 behaves differently in meiosis and mitosis. However, the function of Emi1 as a CSF component was further called into question when Kishimoto and colleagues reported that endogenous Emi1 is undetectable in CSF-arrested Xenopus oocytes (Ohsumi et al., 2004). Furthermore, these ...
Characterization of chloroplasts in pavement cells of Arabidopsis
... size to guard cell chloroplasts but are approximately half the size of mesophyll chloroplasts (MCC). In the jigsaw shaped pavement cells in expanded cotyledons and leaves of Arabidopsis, 9 to 15 PCC are found per cell, while a single mesophyll cell may contain over 120 chloroplasts (Pyke and Leech, ...
... size to guard cell chloroplasts but are approximately half the size of mesophyll chloroplasts (MCC). In the jigsaw shaped pavement cells in expanded cotyledons and leaves of Arabidopsis, 9 to 15 PCC are found per cell, while a single mesophyll cell may contain over 120 chloroplasts (Pyke and Leech, ...
The Use of Conditional Lethal Cell Cycle Mutants for
... function. Second, there is the interval of the cycle during which the gene product in question is normally synthesized. [Note that some gene products that function during discrete intervals of the cycle may be synthesized continuously throughout the cycle, while others (as shown in fig. 3) may be sy ...
... function. Second, there is the interval of the cycle during which the gene product in question is normally synthesized. [Note that some gene products that function during discrete intervals of the cycle may be synthesized continuously throughout the cycle, while others (as shown in fig. 3) may be sy ...
Karyotypes
... • Chromosomal mutation: mutation that changes the number or structure of chromosomes (entire genes not just bases are changed). – Deletion: The loss of all or part of a chromosome – Duplication: A segment of the chromosome is repeated – Inversion: part of the chromosome is reverse from its usual dir ...
... • Chromosomal mutation: mutation that changes the number or structure of chromosomes (entire genes not just bases are changed). – Deletion: The loss of all or part of a chromosome – Duplication: A segment of the chromosome is repeated – Inversion: part of the chromosome is reverse from its usual dir ...
Role of the ABC Transporter Ste6 in Cell Fusion during Yeast
... (reviewed in references 25, 28, 40). Pheromone treatment of non-mating cells leads to cell cycle arrest but not to the cell lysis that might be expected with loss of cell wall or membrane integrity (20) suggesting that the end stages of cell fusion may require special signals. Though the process of ...
... (reviewed in references 25, 28, 40). Pheromone treatment of non-mating cells leads to cell cycle arrest but not to the cell lysis that might be expected with loss of cell wall or membrane integrity (20) suggesting that the end stages of cell fusion may require special signals. Though the process of ...
Mastoparan Alters Subcellular Distribution of
... Staiger et al. 1997, Gibbon and Staiger 2000). Profilin is enriched at peripheral cytoplasmic domains of animal cells which have dynamic AFs that rapidly respond to diverse signals (Buß et al. 1992, Mayboroda et al. 1997). Moreover, birch pollen profilin co-localizes with dynamic AFs when expressed ...
... Staiger et al. 1997, Gibbon and Staiger 2000). Profilin is enriched at peripheral cytoplasmic domains of animal cells which have dynamic AFs that rapidly respond to diverse signals (Buß et al. 1992, Mayboroda et al. 1997). Moreover, birch pollen profilin co-localizes with dynamic AFs when expressed ...
Distinct cathepsins control necrotic cell death
... necrotic cell death, designated as lysosome-mediated necrosis (LMN), that is characterized by lysosome rupture and broad proteolysis of cytosolic proteins.28,35 Intriguingly, the cathepsin Bselective inhibitor, CA-074-Me, blocks not only all known forms of necrotic cell death, but also apoptotic cel ...
... necrotic cell death, designated as lysosome-mediated necrosis (LMN), that is characterized by lysosome rupture and broad proteolysis of cytosolic proteins.28,35 Intriguingly, the cathepsin Bselective inhibitor, CA-074-Me, blocks not only all known forms of necrotic cell death, but also apoptotic cel ...
questionnaire a - Waisman Biomanufacturing
... cGMP Master Cell Bank Research Cell Bank Plasmid DNA Other d. Waisman recommends using either DH5-α or DH-10B strains of E. coli for producing plasmid DNA. Please indicate if your cell bank is different or would prefer using an alternative bacterium or strain of E. coli. ...
... cGMP Master Cell Bank Research Cell Bank Plasmid DNA Other d. Waisman recommends using either DH5-α or DH-10B strains of E. coli for producing plasmid DNA. Please indicate if your cell bank is different or would prefer using an alternative bacterium or strain of E. coli. ...
Biology: Concepts and Connections, 6e (Campbell)
... blood for WBCs, would could you do to reduce interference from RBCs? A) Mix the blood in a salty solution to cause the RBCs to lyse. B) Mix the blood in an isotonic solution and allow the WBCs to float to the top. C) Mix the blood with a dye that stains the proteins in the cytoplasm. D) Mix the bloo ...
... blood for WBCs, would could you do to reduce interference from RBCs? A) Mix the blood in a salty solution to cause the RBCs to lyse. B) Mix the blood in an isotonic solution and allow the WBCs to float to the top. C) Mix the blood with a dye that stains the proteins in the cytoplasm. D) Mix the bloo ...
Regulation of Vascular Development - Size
... differentiate in appropriate numbers and spatial relationships that give a functional whole. Developing vascular tissues must be able to carry out their functions in expanding organs; this is particularly challenging in grasses and other groups of plants where leaves and stems grow from the base, an ...
... differentiate in appropriate numbers and spatial relationships that give a functional whole. Developing vascular tissues must be able to carry out their functions in expanding organs; this is particularly challenging in grasses and other groups of plants where leaves and stems grow from the base, an ...
Cell dynamics of folding in two
... rapidly under appropriate conditions into their native states. It is believed that rapid folders are sequences for which the folding dynamics entail the exploration of restricted conformations — the phase space can be thought of as a folding funnel. While there are many experimentally accessible pre ...
... rapidly under appropriate conditions into their native states. It is believed that rapid folders are sequences for which the folding dynamics entail the exploration of restricted conformations — the phase space can be thought of as a folding funnel. While there are many experimentally accessible pre ...
Cytokinesis
Cytokinesis (cyto- + kinesis) is the process during cell division in which the cytoplasm of a single eukaryotic cell is divided to form two daughter cells. It usually initiates during the early stages of mitosis, and sometimes meiosis, splitting a mitotic cell in two, to ensure that chromosome number is maintained from one generation to the next. After cytokinesis two (daughter) cells will be formed that are exact copies of the (parent) original cell. After cytokinesis, each daughter cell is in the interphase portion of the cell cycle. In animal cells, one notable exception to the normal process of cytokinesis is oogenesis (the creation of an ovum in the ovarian follicle of the ovary), where the ovum takes almost all the cytoplasm and organelles, leaving very little for the resulting polar bodies, which then die. Another form of mitosis without cytokinesis occurs in the liver, yielding multinucleate cells. In plant cells, a dividing structure known as the cell plate forms within the centre of the cytoplasm and a new cell wall forms between the two daughter cells.Cytokinesis is distinguished from the prokaryotic process of binary fission.