Paclitaxel-induced microtubule stabilization causes mitotic block
... strain. In the presence of paclitaxel, cytoplasmic microtubules were stable to cold depolymerization. Paclitaxel-treated cells showed evidence of a mitotic block, with an increase in large-budded cells and cells with a 2N DNA content and DNA fragmentation, identified by FACS analysis and the TUNEL a ...
... strain. In the presence of paclitaxel, cytoplasmic microtubules were stable to cold depolymerization. Paclitaxel-treated cells showed evidence of a mitotic block, with an increase in large-budded cells and cells with a 2N DNA content and DNA fragmentation, identified by FACS analysis and the TUNEL a ...
ERdj5, an Endoplasmic Reticulum (ER)
... Catalysis of oxidative folding is necessary to rapidly generate the correct disulfide bonds in newly synthesized proteins (15). A crucial component of the ER folding machinery is PDI. This multifunctional protein catalyzes disulfide bond formation and isomerization within the ER and displays both ch ...
... Catalysis of oxidative folding is necessary to rapidly generate the correct disulfide bonds in newly synthesized proteins (15). A crucial component of the ER folding machinery is PDI. This multifunctional protein catalyzes disulfide bond formation and isomerization within the ER and displays both ch ...
The linkage between cell wall metabolism and fruit
... release of oligosaccharides from a polymer followed by their derivatisation with a fluorophore and then separation by electrophoresis in polyacrylamide gels.21 The migration of different oligosaccharides will depend on their mass and monosaccharide composition, so polysaccharides will generate a spe ...
... release of oligosaccharides from a polymer followed by their derivatisation with a fluorophore and then separation by electrophoresis in polyacrylamide gels.21 The migration of different oligosaccharides will depend on their mass and monosaccharide composition, so polysaccharides will generate a spe ...
PPT - 서울대 Biointelligence lab
... The key to ICPs lies in the recurrent nature of neural circuits. Repeated co-activation of a group of neurons during behavior will lead to the formation of a cell assembly – an anatomically dispersed set of neurons amongst which excitatory connections have been potentiated. © 2008 SNU CSE Biointelli ...
... The key to ICPs lies in the recurrent nature of neural circuits. Repeated co-activation of a group of neurons during behavior will lead to the formation of a cell assembly – an anatomically dispersed set of neurons amongst which excitatory connections have been potentiated. © 2008 SNU CSE Biointelli ...
CRISPR/Cas9 Screens Reveal Requirements for Host Cell Sulfation
... Letchumanan et al., 2014). All V. parahaemolyticus strains harbor genes encoding a T3SS on their large chromosome, T3SS1. Nearly all clinical isolates produce a second, evolutionarily distinct T3SS, T3SS2 (Abby and Rocha, 2012; Hazen et al., 2015), which is encoded within a pathogenicity island on t ...
... Letchumanan et al., 2014). All V. parahaemolyticus strains harbor genes encoding a T3SS on their large chromosome, T3SS1. Nearly all clinical isolates produce a second, evolutionarily distinct T3SS, T3SS2 (Abby and Rocha, 2012; Hazen et al., 2015), which is encoded within a pathogenicity island on t ...
Nucleation and Dynamics of Golgi-derived Microtubules
... obviously numerous and probably redundant, likely serve to extend Golgi-derived MT lifetime, allowing for their robust function. Thus, we consider MT stabilization as an important step in MT formation at the Golgi (Figure 1). That being said, while we have grouped known molecular factors in two grou ...
... obviously numerous and probably redundant, likely serve to extend Golgi-derived MT lifetime, allowing for their robust function. Thus, we consider MT stabilization as an important step in MT formation at the Golgi (Figure 1). That being said, while we have grouped known molecular factors in two grou ...
Full-Text PDF
... also revealed that the regenerating central vacuole responsible for cell turgor is generated mainly by the lytic vacuolar compartment, labeled by AleuGFP. In contrast, GFPChi labeled small peripheral vacuoles, which we called pre-vacuoles. These fused with the central vacuole in a second step, proba ...
... also revealed that the regenerating central vacuole responsible for cell turgor is generated mainly by the lytic vacuolar compartment, labeled by AleuGFP. In contrast, GFPChi labeled small peripheral vacuoles, which we called pre-vacuoles. These fused with the central vacuole in a second step, proba ...
PDF
... chaperones, such as HJURP in mammals, Scm3 in fission yeast, Sim3 in budding yeast (reviewed in De Rop et al., 2012) or more recently CAL1 in Drosophila (Chen et al., 2014), were found to be required for CENH3 deposition at centromeres. Each of these chaperones was shown to selectively bind CENH3, an ...
... chaperones, such as HJURP in mammals, Scm3 in fission yeast, Sim3 in budding yeast (reviewed in De Rop et al., 2012) or more recently CAL1 in Drosophila (Chen et al., 2014), were found to be required for CENH3 deposition at centromeres. Each of these chaperones was shown to selectively bind CENH3, an ...
Pomegranate Ellagitannin–Derived Compounds Exhibit
... that both UA and UB were taken up intact in MCF-7 cells and metabolized to their sulfate and glucuronide forms; however, UB uptake was shown to be greater than that of UA. This result could explain the lower activity of UA in the live cell system. In addition to the differences in UB and UA absorpti ...
... that both UA and UB were taken up intact in MCF-7 cells and metabolized to their sulfate and glucuronide forms; however, UB uptake was shown to be greater than that of UA. This result could explain the lower activity of UA in the live cell system. In addition to the differences in UB and UA absorpti ...
Encontro de Jovens Investigadores de Biologia Computacional
... electrochemical proton gradient. Over the last 20 years, most of the CCOX research focused on the mechanisms and energetics of reduction and/or proton pumping but, until now, these mechanisms are still elusive. In particular, it is still not clear which are the functionally relevant conformational c ...
... electrochemical proton gradient. Over the last 20 years, most of the CCOX research focused on the mechanisms and energetics of reduction and/or proton pumping but, until now, these mechanisms are still elusive. In particular, it is still not clear which are the functionally relevant conformational c ...
Simian Virus 40 Large T Antigen and p53 Are
... 5, 8, and 9) or 1.0 g (Lanes 2, 3, 6, 7, and 10). Authentic p53 (Lanes 1 and 2), M, 56,000 and 8), and M, 45,000 (Lanes 9 and 10) proteins ...
... 5, 8, and 9) or 1.0 g (Lanes 2, 3, 6, 7, and 10). Authentic p53 (Lanes 1 and 2), M, 56,000 and 8), and M, 45,000 (Lanes 9 and 10) proteins ...
Review Cell Death Signalling Pathways in the
... Abstract. Apoptosis, a Greek descriptive term for falling leaves or petals, plays an important role in the progression of many diseases. Apoptosis is essential for the development and survival of multi-cellular organisms. Malignant diseases, including haematologic malignancies, are associated with d ...
... Abstract. Apoptosis, a Greek descriptive term for falling leaves or petals, plays an important role in the progression of many diseases. Apoptosis is essential for the development and survival of multi-cellular organisms. Malignant diseases, including haematologic malignancies, are associated with d ...
Notochord morphogenesis in Xenopus laevis
... ically coupled cells within a tissue. The rules of eel behavior control the mechanics of individual cells, sc that when a large number of cells are coupled to one another, we can compare the development of the simulated tissue to morphometric data from scanning electron micrographs and time-lapse re ...
... ically coupled cells within a tissue. The rules of eel behavior control the mechanics of individual cells, sc that when a large number of cells are coupled to one another, we can compare the development of the simulated tissue to morphometric data from scanning electron micrographs and time-lapse re ...
Transcription- and translation-dependent changes in membrane
... hand, there was no difference in residual divisions (about ...
... hand, there was no difference in residual divisions (about ...
Lipids of the Plant Plasma Membrane
... Isolating highly purified fractions of organelles or membranes from other cellular compartments is a key requirement for in-depth identification and characterization of membrane proteins and lipids. PM fractions were first purified from microsomal membranes (a mix of various cellular membranes) by t ...
... Isolating highly purified fractions of organelles or membranes from other cellular compartments is a key requirement for in-depth identification and characterization of membrane proteins and lipids. PM fractions were first purified from microsomal membranes (a mix of various cellular membranes) by t ...
Interaction with PI3-kinase contributes to the cytotoxic activity ofApoptin S Maddika
... apoptin is found in the nucleus (Danen-Van Oorschot et al., 2003). Apoptin was described to be specifically phosphorylated in transformed cells by an unknown kinase, which was proposed to be important for the nuclear localization and apoptotic activity of apoptin (Rohn et al., 2002). However, it was ...
... apoptin is found in the nucleus (Danen-Van Oorschot et al., 2003). Apoptin was described to be specifically phosphorylated in transformed cells by an unknown kinase, which was proposed to be important for the nuclear localization and apoptotic activity of apoptin (Rohn et al., 2002). However, it was ...
4T, 3T AND 3T1D DRAM CELL DESIGN ON 32 NM
... threshold voltage and physical dimensions (length and width) of transistors. These variations can be classified depending on their statistics as systematic (inter die and intra-die systematic) or random (intra-die random). To simulate these effects in a single cell we use a Gaussian distribution. Sy ...
... threshold voltage and physical dimensions (length and width) of transistors. These variations can be classified depending on their statistics as systematic (inter die and intra-die systematic) or random (intra-die random). To simulate these effects in a single cell we use a Gaussian distribution. Sy ...
Site directed mutagenesis of Drosophila flightin disrupts
... that is contained within the LMM fragment (residues 1506–1674) that has been shown to bind the M band proteins myomesin and M-Protein (Obermann et al. 1997, 1998). Thus, a small segment of the myosin coiled coil appears to have been evolutionarily conserved as a site of myosin binding protein intera ...
... that is contained within the LMM fragment (residues 1506–1674) that has been shown to bind the M band proteins myomesin and M-Protein (Obermann et al. 1997, 1998). Thus, a small segment of the myosin coiled coil appears to have been evolutionarily conserved as a site of myosin binding protein intera ...
Journal of Bacteriology
... this pleiotropic effect causes the altered phenotype with respect to attachment. The Ca2"-dependent adhesin of Rhizobwum appears to be a soluble surface protein. The supernatant, and not the flagellum-containing pellet, obtained after the ultracentrifugation step in flagellum purification appeared t ...
... this pleiotropic effect causes the altered phenotype with respect to attachment. The Ca2"-dependent adhesin of Rhizobwum appears to be a soluble surface protein. The supernatant, and not the flagellum-containing pellet, obtained after the ultracentrifugation step in flagellum purification appeared t ...
Document
... Homologous chromosomes physically connect and exchange genetic information – At the metaphase plate, there are paired homologous chromosomes (tetrads), instead of individual replicated chromosomes – At anaphase I, it is homologous chromosomes, instead of sister chromatids, that separate ...
... Homologous chromosomes physically connect and exchange genetic information – At the metaphase plate, there are paired homologous chromosomes (tetrads), instead of individual replicated chromosomes – At anaphase I, it is homologous chromosomes, instead of sister chromatids, that separate ...
Plant Cell Vacuoles
... via the Golgi apparatus, as indicated by the typical modifications of glycans. Vesicles containing vacuolar proteins have also been visualized in the process of budding from the Golgi apparatus. Vacuolar proteins harbour positive sorting information that diverts them from the default pathway for solu ...
... via the Golgi apparatus, as indicated by the typical modifications of glycans. Vesicles containing vacuolar proteins have also been visualized in the process of budding from the Golgi apparatus. Vacuolar proteins harbour positive sorting information that diverts them from the default pathway for solu ...
RtoA links initial cell type choice to the cell cycle in
... involved in these mechanisms are active at the time of starvation but are not required for cell division, the screen was designed to select for genes that are expressed in vegetative cells, and when repressed affect development but not growth. A cDNA library was placed downstream of a vegetative pro ...
... involved in these mechanisms are active at the time of starvation but are not required for cell division, the screen was designed to select for genes that are expressed in vegetative cells, and when repressed affect development but not growth. A cDNA library was placed downstream of a vegetative pro ...
C2006/F2402 `07
... C. In people with the genetic condition called Liddle’s syndrome, there is too much Na+ transport. Each individual channel transports Na+ normally, but there is more overall transport than normal. (This causes high blood pressure.) People with Liddle’s syndrome have ENaC that is missing the last 45- ...
... C. In people with the genetic condition called Liddle’s syndrome, there is too much Na+ transport. Each individual channel transports Na+ normally, but there is more overall transport than normal. (This causes high blood pressure.) People with Liddle’s syndrome have ENaC that is missing the last 45- ...
An Abscisic Acid-Activated and Calcium-lndependent
... and to ABA by shrinking (data not shown), as reported previously (Fitzsimons and Weyers, 1986,1987), demonstrating that they are competent to respond to these signals. Proteins from GCPs were assayed for autophosphorylation activity by an in-gel assay (see Methods). When the gel was incubated with y ...
... and to ABA by shrinking (data not shown), as reported previously (Fitzsimons and Weyers, 1986,1987), demonstrating that they are competent to respond to these signals. Proteins from GCPs were assayed for autophosphorylation activity by an in-gel assay (see Methods). When the gel was incubated with y ...
Escherichia coli Evolutionary-conserved and Essential*
... periplasmic region downstream of the first TMs is neither conserved nor essential for YidC functioning per se (23, 36). The fused Oxa1 part consists of the conserved mature Oxa1, lacking its matrix targeting sequence. The hybrid construct was named ecOxa1 for E. coli-targeted Oxa1 and was cloned und ...
... periplasmic region downstream of the first TMs is neither conserved nor essential for YidC functioning per se (23, 36). The fused Oxa1 part consists of the conserved mature Oxa1, lacking its matrix targeting sequence. The hybrid construct was named ecOxa1 for E. coli-targeted Oxa1 and was cloned und ...
Cytokinesis
Cytokinesis (cyto- + kinesis) is the process during cell division in which the cytoplasm of a single eukaryotic cell is divided to form two daughter cells. It usually initiates during the early stages of mitosis, and sometimes meiosis, splitting a mitotic cell in two, to ensure that chromosome number is maintained from one generation to the next. After cytokinesis two (daughter) cells will be formed that are exact copies of the (parent) original cell. After cytokinesis, each daughter cell is in the interphase portion of the cell cycle. In animal cells, one notable exception to the normal process of cytokinesis is oogenesis (the creation of an ovum in the ovarian follicle of the ovary), where the ovum takes almost all the cytoplasm and organelles, leaving very little for the resulting polar bodies, which then die. Another form of mitosis without cytokinesis occurs in the liver, yielding multinucleate cells. In plant cells, a dividing structure known as the cell plate forms within the centre of the cytoplasm and a new cell wall forms between the two daughter cells.Cytokinesis is distinguished from the prokaryotic process of binary fission.