Recent Advances in αβ T Cell Biology: Wnt Signaling
... signaling depends on the γ-secretase-mediated release of the Notch intracellular domain (NICD) from the cell membrane. This allows the translocation of NICD into the nucleus, where it directly participates in a core transcriptional complex with the DNA-binding protein Suppressor of Hairless (SU(H)) ...
... signaling depends on the γ-secretase-mediated release of the Notch intracellular domain (NICD) from the cell membrane. This allows the translocation of NICD into the nucleus, where it directly participates in a core transcriptional complex with the DNA-binding protein Suppressor of Hairless (SU(H)) ...
The organic anion transport inhibitor, probenecid, inhibits the
... Because of the properties listed above, LY-CH has been used to demonstrate fluid-phase endocytosis in cultured animal cells (Miller et al. 1983; Swanson et al. 1985, 1986, 1987; Bernardini et al. 1986, 1987; McKinley and Wiley, 1988; Cosson et al. 1989). In these studies, the probe was sequestered r ...
... Because of the properties listed above, LY-CH has been used to demonstrate fluid-phase endocytosis in cultured animal cells (Miller et al. 1983; Swanson et al. 1985, 1986, 1987; Bernardini et al. 1986, 1987; McKinley and Wiley, 1988; Cosson et al. 1989). In these studies, the probe was sequestered r ...
Cells
... to extract intact ER starts the biosynthetic pathways form many protein and lipid molecules in the cell These continue in the Golgi apparatus Rough ER has ribosomes attached to it as opposed to Smooth ER The proteins are made on rough ER will eventually be secreted outside the cell ...
... to extract intact ER starts the biosynthetic pathways form many protein and lipid molecules in the cell These continue in the Golgi apparatus Rough ER has ribosomes attached to it as opposed to Smooth ER The proteins are made on rough ER will eventually be secreted outside the cell ...
Type study Paramecium
... starts off in a new direction. Paramecia feed mostly on bacteria, which are driven into the gullet by the cilia. Two contractile vacuoles regulate osmotic pressure (see Osmosis) and also serve as excretory structures. ...
... starts off in a new direction. Paramecia feed mostly on bacteria, which are driven into the gullet by the cilia. Two contractile vacuoles regulate osmotic pressure (see Osmosis) and also serve as excretory structures. ...
An ARL1 mutation affected autophagic cell death in yeast
... death essential for the development and homeostasis of multicellular organisms. However, in unicellular organisms like yeast, the expression of a number of proapoptotic mammalian genes like bax and p53 leads to cell death, and the cell death is inhibited by expression of mammalian antiapoptotic gene ...
... death essential for the development and homeostasis of multicellular organisms. However, in unicellular organisms like yeast, the expression of a number of proapoptotic mammalian genes like bax and p53 leads to cell death, and the cell death is inhibited by expression of mammalian antiapoptotic gene ...
BOSTON UNIVERSITY SCHOOL OF MEDICINE Thesis THE ROLE
... that newly generated granule neurons found in the olfactory bulb migrate to their final destination by way of the rostral migratory stream (Altman 1969). The scientific community largely ignored these results believing they lacked any functional significance. It wasn’t until the late 1970s that neur ...
... that newly generated granule neurons found in the olfactory bulb migrate to their final destination by way of the rostral migratory stream (Altman 1969). The scientific community largely ignored these results believing they lacked any functional significance. It wasn’t until the late 1970s that neur ...
Myc Requires Distinct E2F Activities to Induce S Phase
... Myc and these E2F genes, employing two assays for Myc function: the ability of Myc to induce entry into S phase and the ability of Myc to induce apoptosis. In all experiments, we tested multiple MEF preparations individually to ensure the reproducibility of the results. E2F2 and E2F3 but Not E2F1 or ...
... Myc and these E2F genes, employing two assays for Myc function: the ability of Myc to induce entry into S phase and the ability of Myc to induce apoptosis. In all experiments, we tested multiple MEF preparations individually to ensure the reproducibility of the results. E2F2 and E2F3 but Not E2F1 or ...
RalA ACTIVATION ASSAY BIOCHEM KIT
... GTP bound (active) RalA is a labile entity, the bound GTP is susceptible to hydrolysis by RalGAPs during and after cell lysis, resulting in RalA inactivation. Rapid processing at 4°C is essential for accurate and reproducible results. The following guidelines are useful for rapid washing of cells. W ...
... GTP bound (active) RalA is a labile entity, the bound GTP is susceptible to hydrolysis by RalGAPs during and after cell lysis, resulting in RalA inactivation. Rapid processing at 4°C is essential for accurate and reproducible results. The following guidelines are useful for rapid washing of cells. W ...
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... involved in both pseudohyphal and haploid invasive growth (Gavrias et al., 1996; Madhani and Fink, 1997; Mosch and Fink, 1997). Other proteins including transcription factors such as Phd1p (Gimeno and Fink, 1994) and Flo8p (Liu et al., 1996), as well as the protein kinase ELM1 (Blacketer et al., 199 ...
... involved in both pseudohyphal and haploid invasive growth (Gavrias et al., 1996; Madhani and Fink, 1997; Mosch and Fink, 1997). Other proteins including transcription factors such as Phd1p (Gimeno and Fink, 1994) and Flo8p (Liu et al., 1996), as well as the protein kinase ELM1 (Blacketer et al., 199 ...
Limbal stem cell deficiency and its management
... Medical or conservative: Lubricants (preservative free) and bandage contact lens wear are the mainstay. Topical steroids and antibiotics (preservative free) for periods of significant ocular surface inflammation and epithelial breakdown. Autologous serum drops have a role in epithelial wound healing ...
... Medical or conservative: Lubricants (preservative free) and bandage contact lens wear are the mainstay. Topical steroids and antibiotics (preservative free) for periods of significant ocular surface inflammation and epithelial breakdown. Autologous serum drops have a role in epithelial wound healing ...
Division, and Morphogenesis in Plants
... bryo is partitioned in regions that will give rise to the seedling shoot apical meristem, embryonic leaves (cotyledons), hypocotyl, root, and root apical meristem (Figs. 2D and 2E). The prospective organ primordia do not correlate with the three early domains, showing that the final definition of or ...
... bryo is partitioned in regions that will give rise to the seedling shoot apical meristem, embryonic leaves (cotyledons), hypocotyl, root, and root apical meristem (Figs. 2D and 2E). The prospective organ primordia do not correlate with the three early domains, showing that the final definition of or ...
Using intrinsically fluorescent proteins for plant cell
... optical characteristics of the IFPs themselves should all significantly impact on this decision. Each of these areas will be discussed in this article. Chimeric gene design, promoter choice and validation of the reporter The location of the IFP in the chimera, the use of linker sequences as spacers ...
... optical characteristics of the IFPs themselves should all significantly impact on this decision. Each of these areas will be discussed in this article. Chimeric gene design, promoter choice and validation of the reporter The location of the IFP in the chimera, the use of linker sequences as spacers ...
Connexin 43 mimetic peptide Gap27 reveals potential differences in
... correlated with decreased gap junctional intercellular communication (GJIC) in cultured human keratinocytes and fibroblasts from young healthy donors [12]. Gap27 is a small synthetic connexin mimetic peptide with a sequence homology to the second extracellular loop of Cx43 [13, 14] and is a benign, ...
... correlated with decreased gap junctional intercellular communication (GJIC) in cultured human keratinocytes and fibroblasts from young healthy donors [12]. Gap27 is a small synthetic connexin mimetic peptide with a sequence homology to the second extracellular loop of Cx43 [13, 14] and is a benign, ...
Skeletal muscle cells
... Muscle Regeneration and Growth Skeletal Muscle • Increase in size (hypertrophy) • Increase in number (regeneration/proliferation) • Satellite cells are proposed source of regenerative cells Smooth Muscle • Increase in size (hypertrophy) • Increase in number (regeneration/proliferation) • Smooth mus ...
... Muscle Regeneration and Growth Skeletal Muscle • Increase in size (hypertrophy) • Increase in number (regeneration/proliferation) • Satellite cells are proposed source of regenerative cells Smooth Muscle • Increase in size (hypertrophy) • Increase in number (regeneration/proliferation) • Smooth mus ...
Presence of exposed phospholipids in the outer membrane of Vibrio
... were done as described by Kamio & Nikaido (1976). Cells were labelled by growing them for 3 generations in media containing [2-3Hlglycerol [specific activity: 1 Ci (37 GBq) mmol-l, 2pCi (74 kBq) ml-l] followed by suspension and shaking for 30 min in fresh growth media containing 0.05 % glycerol. The ...
... were done as described by Kamio & Nikaido (1976). Cells were labelled by growing them for 3 generations in media containing [2-3Hlglycerol [specific activity: 1 Ci (37 GBq) mmol-l, 2pCi (74 kBq) ml-l] followed by suspension and shaking for 30 min in fresh growth media containing 0.05 % glycerol. The ...
Transient pluripotent cell populations in vivo
... (Haub and Goldfarb, 1991; Hebert et al., 1991), Rex1 (Rogers et al., 1991) and Gbx2 (Chapman et al., 1997) genes are expressed differently in the ICM and primitive ectoderm. Moreover, the temporal expression of these genes in the pluripotent cell pool in vivo has only been crudely assessed. We have ...
... (Haub and Goldfarb, 1991; Hebert et al., 1991), Rex1 (Rogers et al., 1991) and Gbx2 (Chapman et al., 1997) genes are expressed differently in the ICM and primitive ectoderm. Moreover, the temporal expression of these genes in the pluripotent cell pool in vivo has only been crudely assessed. We have ...
Putative Autocleavage of Outer Capsid Protein 1, Allowing Release
... the outer capsid protein 2 is responsible for capping the 5⬘ end of each viral plus-strand RNA as it exits the particle during transcription (34; reviewed in reference 58). The other outer capsid proteins—1, 3, and 1—are involved in cell entry. The majority of the outer capsid lattice is formed ...
... the outer capsid protein 2 is responsible for capping the 5⬘ end of each viral plus-strand RNA as it exits the particle during transcription (34; reviewed in reference 58). The other outer capsid proteins—1, 3, and 1—are involved in cell entry. The majority of the outer capsid lattice is formed ...
The Biotechnology Century and Its Workforce
... Spheroplast is a wall-less gram-positive cell Protoplasts and spheroplasts are susceptible to osmotic lysis ...
... Spheroplast is a wall-less gram-positive cell Protoplasts and spheroplasts are susceptible to osmotic lysis ...
Interaction of oxygen-sensitive luminescent probes Ru(phen) and
... freely pass through intact biological membranes. Therefore, they could be deliberately confined to the extracellular space or to endosomes. Moreover, luminescent signals should be sufficiently strong to perform measurements of phase shift and further calculations of luminescence lifetimes [8]. Measu ...
... freely pass through intact biological membranes. Therefore, they could be deliberately confined to the extracellular space or to endosomes. Moreover, luminescent signals should be sufficiently strong to perform measurements of phase shift and further calculations of luminescence lifetimes [8]. Measu ...
Convergence and Extension Movements During Vertebrate
... dorsal convergence (Sepich et al., 2005). Starting from midgastrulation (7.5 hpf ), the progenitors of all three germ layers undergo C&E movements (Concha and Adams, 1998; Pezeron et al., 2008; Sepich et al., 2005). Whereas the pattern of endoderm and ectoderm C&E is less understood, four distinct C ...
... dorsal convergence (Sepich et al., 2005). Starting from midgastrulation (7.5 hpf ), the progenitors of all three germ layers undergo C&E movements (Concha and Adams, 1998; Pezeron et al., 2008; Sepich et al., 2005). Whereas the pattern of endoderm and ectoderm C&E is less understood, four distinct C ...
ATCC® EnTEriC DisEAsE rEsEArCh MATEriAls
... The ATCC® Genuine Nucleics collection represents the largest and most diverse array of both cellular and microbiological genomic, synthetic, and certified reference materials available worldwide. Each preparation is isolated or synthetically derived under aseptic conditions to prevent cross-contamin ...
... The ATCC® Genuine Nucleics collection represents the largest and most diverse array of both cellular and microbiological genomic, synthetic, and certified reference materials available worldwide. Each preparation is isolated or synthetically derived under aseptic conditions to prevent cross-contamin ...
Phorbol Esters Alter Cell Fate during Development of Sea Urchin
... triradiate spicules were formed in the blastocoel (Fig. 2 E). Gut formation in sea urchin normally occurs by the invagination of prospective endoderm at the vegetal pole. In TPAtreated embryos, gut tissue can be formed by invagination (or by exogastrulation), but is sometimes formed by the aggregati ...
... triradiate spicules were formed in the blastocoel (Fig. 2 E). Gut formation in sea urchin normally occurs by the invagination of prospective endoderm at the vegetal pole. In TPAtreated embryos, gut tissue can be formed by invagination (or by exogastrulation), but is sometimes formed by the aggregati ...
Paclitaxel-induced microtubule stabilization causes mitotic block
... strain. In the presence of paclitaxel, cytoplasmic microtubules were stable to cold depolymerization. Paclitaxel-treated cells showed evidence of a mitotic block, with an increase in large-budded cells and cells with a 2N DNA content and DNA fragmentation, identified by FACS analysis and the TUNEL a ...
... strain. In the presence of paclitaxel, cytoplasmic microtubules were stable to cold depolymerization. Paclitaxel-treated cells showed evidence of a mitotic block, with an increase in large-budded cells and cells with a 2N DNA content and DNA fragmentation, identified by FACS analysis and the TUNEL a ...
Cytokinesis
Cytokinesis (cyto- + kinesis) is the process during cell division in which the cytoplasm of a single eukaryotic cell is divided to form two daughter cells. It usually initiates during the early stages of mitosis, and sometimes meiosis, splitting a mitotic cell in two, to ensure that chromosome number is maintained from one generation to the next. After cytokinesis two (daughter) cells will be formed that are exact copies of the (parent) original cell. After cytokinesis, each daughter cell is in the interphase portion of the cell cycle. In animal cells, one notable exception to the normal process of cytokinesis is oogenesis (the creation of an ovum in the ovarian follicle of the ovary), where the ovum takes almost all the cytoplasm and organelles, leaving very little for the resulting polar bodies, which then die. Another form of mitosis without cytokinesis occurs in the liver, yielding multinucleate cells. In plant cells, a dividing structure known as the cell plate forms within the centre of the cytoplasm and a new cell wall forms between the two daughter cells.Cytokinesis is distinguished from the prokaryotic process of binary fission.