Database Machine
... Success of SAN caches is built upon predictive analytics They work well, if a small percentage of disk accessed most often - The emphasis is on disk; not data Most database systems - Are way bigger than caches - Need to get the data to the memory to process --> I/O at the disk level is still h ...
... Success of SAN caches is built upon predictive analytics They work well, if a small percentage of disk accessed most often - The emphasis is on disk; not data Most database systems - Are way bigger than caches - Need to get the data to the memory to process --> I/O at the disk level is still h ...
Ciliary Microtubule Capping Structures Contain A
... affinity-purified antibodies were tested by immunoblotting as shown in Fig. 4 (lanes 3 and 3') and found to bind exclusively to the 97-kD protein in the cap fraction and to no proteins in the axonemal fraction. The 34-kD polypeptide that occasionally stained in the capping or axonemal fraction was n ...
... affinity-purified antibodies were tested by immunoblotting as shown in Fig. 4 (lanes 3 and 3') and found to bind exclusively to the 97-kD protein in the cap fraction and to no proteins in the axonemal fraction. The 34-kD polypeptide that occasionally stained in the capping or axonemal fraction was n ...
Surface CD81 Via Microparticles Release and Intercellular Transfer
... was also observed in the CD4-positive Jurkat T cell line. We chose Jurkat T cells because of their known responsiveness to anti-CD3 and anti-CD28 mAbs and CD81 surface levels in the same range as primary cells (Fig. 1A). The reduction of surface CD81 occurred as early as 1 h after activation and pro ...
... was also observed in the CD4-positive Jurkat T cell line. We chose Jurkat T cells because of their known responsiveness to anti-CD3 and anti-CD28 mAbs and CD81 surface levels in the same range as primary cells (Fig. 1A). The reduction of surface CD81 occurred as early as 1 h after activation and pro ...
Biological effects of 6 mT static magnetic fields: A comparative study
... A deeper knowledge of the negative and/or positive effects of MFs on cellular processes, including apoptosis is, therefore, important. The unbalance of the apoptotic process could be linked to Ca2þ fluxes that are, in turn, dependent on the effect on the plasma membrane exerted by static MF. In fact ...
... A deeper knowledge of the negative and/or positive effects of MFs on cellular processes, including apoptosis is, therefore, important. The unbalance of the apoptotic process could be linked to Ca2þ fluxes that are, in turn, dependent on the effect on the plasma membrane exerted by static MF. In fact ...
Gram Staining - WordPress.com
... by their cell wall components. The procedure distinguishes between two groups : Gram Positive and Gram Negative by staining them red or violet. Gram Positive bacteria have a thicker layer of peptidoglycan in their cell wall, so they stain violet because the substance retains the crystal violet stain ...
... by their cell wall components. The procedure distinguishes between two groups : Gram Positive and Gram Negative by staining them red or violet. Gram Positive bacteria have a thicker layer of peptidoglycan in their cell wall, so they stain violet because the substance retains the crystal violet stain ...
polyribosomes
... Conveys specific amino acids to site of protein syn thesis. Each amino acid has its own type of transfel RNA. ...
... Conveys specific amino acids to site of protein syn thesis. Each amino acid has its own type of transfel RNA. ...
Protein Import, Replication, and Inheritance of a Vestigial
... that division and segregation of a single centrally positioned mitosome tightly associated with the microtubular cytoskeleton is coordinated with the cell cycle, whereas peripherally located mitosomes are inherited into daughter cells stochastically. ...
... that division and segregation of a single centrally positioned mitosome tightly associated with the microtubular cytoskeleton is coordinated with the cell cycle, whereas peripherally located mitosomes are inherited into daughter cells stochastically. ...
Sec35p, a Novel Peripheral Membrane Protein, Is Required for ER
... negative regulator of v/t-SNARE complex assembly, perhaps by preventing the association of the v- and t-SNAREs ...
... negative regulator of v/t-SNARE complex assembly, perhaps by preventing the association of the v- and t-SNAREs ...
Paramecium
... Paramecium are unicellular protozoans classified in the phylum Ciliophora (pronounced sill-eeuh-FORE-uh), and the Kingdom Protista. Recall that protozoans are animal-like protists, named that way because they move and eat just like animals only they are made of a single cell. Paramecia live in quiet ...
... Paramecium are unicellular protozoans classified in the phylum Ciliophora (pronounced sill-eeuh-FORE-uh), and the Kingdom Protista. Recall that protozoans are animal-like protists, named that way because they move and eat just like animals only they are made of a single cell. Paramecia live in quiet ...
Primary cilia of human endothelial cells disassemble under laminar
... was part of a major rearrangement of the cytoskeleton. It coincided with changes in the acetylation of microtubules and in the organization of microfilaments (Wojciak-Stothard and Ridley, 2003). It was complete and not partial, as observed for primary cilia subjected to low temperature or trypsiniza ...
... was part of a major rearrangement of the cytoskeleton. It coincided with changes in the acetylation of microtubules and in the organization of microfilaments (Wojciak-Stothard and Ridley, 2003). It was complete and not partial, as observed for primary cilia subjected to low temperature or trypsiniza ...
Regulation of the Discs Large Tumor Suppressor by a
... (17, 18). Although some hDlg isoforms are associated with the cell membrane, others show nuclear localization, possibly performing signaling functions (19). Similarly to its Drosophila homolog, hDlg appears to have dual roles in governing both polarity and proliferation of epithelial cells. It has b ...
... (17, 18). Although some hDlg isoforms are associated with the cell membrane, others show nuclear localization, possibly performing signaling functions (19). Similarly to its Drosophila homolog, hDlg appears to have dual roles in governing both polarity and proliferation of epithelial cells. It has b ...
20. A Cellular Adventure
... Teacher: Today we are going to look at eukaryote cells. Let me pass out the direction so we can get started. Joe: Eureka cells? What are those? Jenny: Eukaryote not eureka. Eukaryote cells are cells that have a nucleus and organelles surrounded by a membrane. Joe: Oh, I get it. Teacher: Look through ...
... Teacher: Today we are going to look at eukaryote cells. Let me pass out the direction so we can get started. Joe: Eureka cells? What are those? Jenny: Eukaryote not eureka. Eukaryote cells are cells that have a nucleus and organelles surrounded by a membrane. Joe: Oh, I get it. Teacher: Look through ...
Reactive oxygen species promote chloroplast dysfunction and
... mechanism by which FB1 regulates light-dependent plant PCD is still largely unknown. Salicylic acid (SA) is a hormone signaling molecule functioning in plant-microbe interactions in the induction of HR. In addition, SA synthesis is influenced by the light condition and closely associated with the chl ...
... mechanism by which FB1 regulates light-dependent plant PCD is still largely unknown. Salicylic acid (SA) is a hormone signaling molecule functioning in plant-microbe interactions in the induction of HR. In addition, SA synthesis is influenced by the light condition and closely associated with the chl ...
Bactericidal activity of the organo
... and causes a six orders of magnitude decrease in viability in a protein-rich medium, but not in a protein-poorer medium, unless 2-mercaptoethanol is added. Subinhibitory concentrations inhibit motility and biofilm formation. AS101 enters the bacterium through its porins and causes bacterial damage t ...
... and causes a six orders of magnitude decrease in viability in a protein-rich medium, but not in a protein-poorer medium, unless 2-mercaptoethanol is added. Subinhibitory concentrations inhibit motility and biofilm formation. AS101 enters the bacterium through its porins and causes bacterial damage t ...
Thermal Diffusion as a Mechanism for Biological
... tween (a) the rate of heat production within the cell and (b) the rate of heat removal from the outer surface of the cell by conduction only. An estimate for the rate of heat production can be m ade from data on the bacterial cells Escherichia coli [36], Production of protein constitutes about 90% o ...
... tween (a) the rate of heat production within the cell and (b) the rate of heat removal from the outer surface of the cell by conduction only. An estimate for the rate of heat production can be m ade from data on the bacterial cells Escherichia coli [36], Production of protein constitutes about 90% o ...
New Insights on Plant Cell Elongation: A Role for Acetylcholine
... could involve secretion via an endocytic vesicle recycling process, at least at the root apex [3–8]. This vesicular secretion closely resembles the synaptic communication in animals [9–12] and, at the same time, supports the neurotransmitter-like concept of auxin [13]. Plants possess many homologous ...
... could involve secretion via an endocytic vesicle recycling process, at least at the root apex [3–8]. This vesicular secretion closely resembles the synaptic communication in animals [9–12] and, at the same time, supports the neurotransmitter-like concept of auxin [13]. Plants possess many homologous ...
The dynamics of plant plasma membrane proteins
... factors appear to function as chaperones primarily in the maturation of non-glycosylated proteins, whereas calreticulin (CRT) controls the fate of glycosylated membrane proteins (Fontes et al., 1991; Denecke et al., 1995; Gupta and Tuteja, 2011). Evidence for the significance of these basic control ...
... factors appear to function as chaperones primarily in the maturation of non-glycosylated proteins, whereas calreticulin (CRT) controls the fate of glycosylated membrane proteins (Fontes et al., 1991; Denecke et al., 1995; Gupta and Tuteja, 2011). Evidence for the significance of these basic control ...
Cell movements driving neuruiation in avian embryos
... further restricted to just two of these stages, shaping and bending of the neural plate, and will be based principally on information obtained, largely during the last five years in my laboratory, from chick and quail embryos. Recent studies have shown that shaping and bending of the neural plate ar ...
... further restricted to just two of these stages, shaping and bending of the neural plate, and will be based principally on information obtained, largely during the last five years in my laboratory, from chick and quail embryos. Recent studies have shown that shaping and bending of the neural plate ar ...
The cell walls of streptococci
... The methods developed by Salton (1953)made possible the study of the cell-wall composition as a relatively simple procedure and recent work by others has suggested that cell-wall composition may be of use in classifying streptococci (Roberts & Stewart, 1961 ; Slade & Slamp, 1962). As part of a gener ...
... The methods developed by Salton (1953)made possible the study of the cell-wall composition as a relatively simple procedure and recent work by others has suggested that cell-wall composition may be of use in classifying streptococci (Roberts & Stewart, 1961 ; Slade & Slamp, 1962). As part of a gener ...
Photoactivation of GFP reveals protein dynamics within the
... observed in GFP-HDEL expressing plants. These sheets remain connected in a network by normal-appearing ER tubules and plants appear unaffected by the change in ER morphology. When activated, the calnexin-PAGFP (CXPAGFP) construct fluorescently marks the ER membrane and, if a small enough region of m ...
... observed in GFP-HDEL expressing plants. These sheets remain connected in a network by normal-appearing ER tubules and plants appear unaffected by the change in ER morphology. When activated, the calnexin-PAGFP (CXPAGFP) construct fluorescently marks the ER membrane and, if a small enough region of m ...
Targeting of Active Sialyltransferase to the Plant Golgi Apparatus
... microscopy. As shown in Figure 4, significant numbers of gold particles were observed over cisternae and membranes closely associated with the Golgi stack in conventionally fixed callus tissue. There was no significant labeling of mitochondria and ER (Figure 4A) or the cell wall and vacuole (Figure ...
... microscopy. As shown in Figure 4, significant numbers of gold particles were observed over cisternae and membranes closely associated with the Golgi stack in conventionally fixed callus tissue. There was no significant labeling of mitochondria and ER (Figure 4A) or the cell wall and vacuole (Figure ...
Cell death in Leishmania induced by stress and differentiation
... degradation have been described.8,12,30 However, none of these reports provide answers about the implicated pathways except in the case of death in Trypanosoma brucei, which was shown to be Ca2+-dependent when induced by reactive oxygen species (ROS). In this case, however, parasites expressing mous ...
... degradation have been described.8,12,30 However, none of these reports provide answers about the implicated pathways except in the case of death in Trypanosoma brucei, which was shown to be Ca2+-dependent when induced by reactive oxygen species (ROS). In this case, however, parasites expressing mous ...
Paper 2
... and have proven fruitful systems for studies of this phenomenon. We have recently shown that tubule extension from vacuoles in hyphae of Pisolithus tinctorius requires the presence of an intact microtubule network [Hyde et al., 1999]. Tubule formation in nearly all endomembrane compartments of other ...
... and have proven fruitful systems for studies of this phenomenon. We have recently shown that tubule extension from vacuoles in hyphae of Pisolithus tinctorius requires the presence of an intact microtubule network [Hyde et al., 1999]. Tubule formation in nearly all endomembrane compartments of other ...
The src
... exhibit various cellular alterations v-src affects a wide variety of cellular targets The first BIG step: using serum to immunoprecipitate the v-Src protein ...
... exhibit various cellular alterations v-src affects a wide variety of cellular targets The first BIG step: using serum to immunoprecipitate the v-Src protein ...
Syndecan-1 regulates αvß5 integrin activity in B82L fibroblasts
... Simons, 2002). The mechanisms by which these domains regulate cell morphology are unknown, although they presumably interact with partners that modulate cellular signaling pathways. One technique for testing syndecan-dependent regulation of cell morphology is to plate cells on syndecan-specific anti ...
... Simons, 2002). The mechanisms by which these domains regulate cell morphology are unknown, although they presumably interact with partners that modulate cellular signaling pathways. One technique for testing syndecan-dependent regulation of cell morphology is to plate cells on syndecan-specific anti ...
Cytokinesis
Cytokinesis (cyto- + kinesis) is the process during cell division in which the cytoplasm of a single eukaryotic cell is divided to form two daughter cells. It usually initiates during the early stages of mitosis, and sometimes meiosis, splitting a mitotic cell in two, to ensure that chromosome number is maintained from one generation to the next. After cytokinesis two (daughter) cells will be formed that are exact copies of the (parent) original cell. After cytokinesis, each daughter cell is in the interphase portion of the cell cycle. In animal cells, one notable exception to the normal process of cytokinesis is oogenesis (the creation of an ovum in the ovarian follicle of the ovary), where the ovum takes almost all the cytoplasm and organelles, leaving very little for the resulting polar bodies, which then die. Another form of mitosis without cytokinesis occurs in the liver, yielding multinucleate cells. In plant cells, a dividing structure known as the cell plate forms within the centre of the cytoplasm and a new cell wall forms between the two daughter cells.Cytokinesis is distinguished from the prokaryotic process of binary fission.