Chapter 6
... • Pores regulate the entry and exit of molecules from the nucleus • The shape of the nucleus is maintained by the nuclear lamina, which is composed of protein ...
... • Pores regulate the entry and exit of molecules from the nucleus • The shape of the nucleus is maintained by the nuclear lamina, which is composed of protein ...
New Insights of Transmembranal Mechanism and Subcellular
... incubation with NaN3, both being able to inhibit endocytosis (Figure 2A). These results strongly indicate the transmembranal pathway for mitochondrial localization of SWNT-PLPEG. We also observed the dispersion of mitochondrial SWNT-PL-PEG into other subcellular components when ∆Ψm decreased by the ...
... incubation with NaN3, both being able to inhibit endocytosis (Figure 2A). These results strongly indicate the transmembranal pathway for mitochondrial localization of SWNT-PLPEG. We also observed the dispersion of mitochondrial SWNT-PL-PEG into other subcellular components when ∆Ψm decreased by the ...
(From tl~ Laboratories of The Rockefeller Institute far Medical
... I t has been found that E. coli B and Staphylococcus muscae after treatment with mustard gas [bis(#-chloroethyl)sulfide] can produce phage but apparently can no longer multiply. Most of the experiments were carried out on the coli system so that this will be discussed first and in more detail. Table ...
... I t has been found that E. coli B and Staphylococcus muscae after treatment with mustard gas [bis(#-chloroethyl)sulfide] can produce phage but apparently can no longer multiply. Most of the experiments were carried out on the coli system so that this will be discussed first and in more detail. Table ...
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... Basel). Raeppli allows whole-tissue labeling such that the descendants of the majority of cells in a single organ are labeled and can be followed simultaneously relative to one another. We tested the use of Raeppli in the Drosophila melanogaster wing imaginal disc. Induction of Raeppli during larval ...
... Basel). Raeppli allows whole-tissue labeling such that the descendants of the majority of cells in a single organ are labeled and can be followed simultaneously relative to one another. We tested the use of Raeppli in the Drosophila melanogaster wing imaginal disc. Induction of Raeppli during larval ...
hSSB1 the guardian of your genome
... the right times. A mutation can change a gene’s instructions for making a protein which can cause the protein to malfunction or to be missing entirely. The most prominent enabling characteristic is the development of genomic instability in cancer cells which generates random mutations. Some mutation ...
... the right times. A mutation can change a gene’s instructions for making a protein which can cause the protein to malfunction or to be missing entirely. The most prominent enabling characteristic is the development of genomic instability in cancer cells which generates random mutations. Some mutation ...
The Rice TAL Effector–Dependent Resistance
... The AvrXa10-dependent Xa10 promoter activity was assayed with a b-glucuronidase (GUS) reporter system (Supplemental Figure 6) and transient expression in Nicotiana benthamiana. The Xa10 promoter, consisting of nucleotides from positions 2220 to 21 (PXa10-220) relative to the transcription start site ...
... The AvrXa10-dependent Xa10 promoter activity was assayed with a b-glucuronidase (GUS) reporter system (Supplemental Figure 6) and transient expression in Nicotiana benthamiana. The Xa10 promoter, consisting of nucleotides from positions 2220 to 21 (PXa10-220) relative to the transcription start site ...
msc_botnay_pre_pap1_bl1 - Madhya Pradesh Bhoj Open
... cell. The surfaces of plant and bacterial cells exhibit many of these same properties, but they also exhibit a few unique features that are not shared by the cells of animals. Plant cell walls provide a supporting framework for intact plants. In addition to providing mechanical support & strength fo ...
... cell. The surfaces of plant and bacterial cells exhibit many of these same properties, but they also exhibit a few unique features that are not shared by the cells of animals. Plant cell walls provide a supporting framework for intact plants. In addition to providing mechanical support & strength fo ...
Golgi clusters and vesicles mediate mitotic inheritance
... Cameron, 1980). More is known about the partitioning process that ensures equal amounts of Golgi apparatus are inherited by each daughter cell (Cabrera-Poch et al., 1998; Shima and Warren, 1998). Most partitioning studies have been carried out using animal cells that locate their Golgi apparatus nea ...
... Cameron, 1980). More is known about the partitioning process that ensures equal amounts of Golgi apparatus are inherited by each daughter cell (Cabrera-Poch et al., 1998; Shima and Warren, 1998). Most partitioning studies have been carried out using animal cells that locate their Golgi apparatus nea ...
The added value of single-cell gene expression
... the large natural variation among individual cells. However, the averaging also eliminates much of the differences in expression contributed by subpopulations. There is also risk that the reference genes selected have different expression profiles in existing subpopulations; the Normfinder algorithm ...
... the large natural variation among individual cells. However, the averaging also eliminates much of the differences in expression contributed by subpopulations. There is also risk that the reference genes selected have different expression profiles in existing subpopulations; the Normfinder algorithm ...
Verrucomicrobia Capturing Single Cell Genomes of Active Polysaccharide
... (AAA168-F10) and two freshwater phylotypes (AAA202-P16 and AAA204-K13) comprised over 2/3 of polysaccharide-positive SAGs in their respective environments. Sequences that were identical or closely related to the most abundant marine and freshwater phylotypes (AAA168-F10 and AAA202-P16) have been rep ...
... (AAA168-F10) and two freshwater phylotypes (AAA202-P16 and AAA204-K13) comprised over 2/3 of polysaccharide-positive SAGs in their respective environments. Sequences that were identical or closely related to the most abundant marine and freshwater phylotypes (AAA168-F10 and AAA202-P16) have been rep ...
microbiology-1st-edition-wessner-test-bank
... Learning Objective: LO 2.4 Identify the components of the bacterial cytoplasmic membrane and cell wall, and the functions of these structures. Section Reference: Section 2.4 The cell envelope 19) What conditions must be met in order for an “active transport system” to transport of a nutrient into a ...
... Learning Objective: LO 2.4 Identify the components of the bacterial cytoplasmic membrane and cell wall, and the functions of these structures. Section Reference: Section 2.4 The cell envelope 19) What conditions must be met in order for an “active transport system” to transport of a nutrient into a ...
DETECTION OF PROTEIN AND mRNA OF
... NADPH oxidase system include three polypeptides of 40, 47 and 67 kDa known as p40-phox, p47-phox and p67-phox, respectively [31–33]. The exact interaction of these three proteins, along with a GTP-binding protein (p21−rac) and the membrane-bound component of the NADPH oxidase system has not been ful ...
... NADPH oxidase system include three polypeptides of 40, 47 and 67 kDa known as p40-phox, p47-phox and p67-phox, respectively [31–33]. The exact interaction of these three proteins, along with a GTP-binding protein (p21−rac) and the membrane-bound component of the NADPH oxidase system has not been ful ...
Evidence for a non-replicative intracellular stage of
... conjugated to rhodamine or Cy2 were purchased from Jackson Immunological and diluted 1 : 200. Fixable dextran 70 000 (molecular mass) labelled with Texas red (TR-dextran) at 25 mg ml21 (Molecular Probes) was used in two types of assays: (i) TR-dextran was used to analyse the uptake of bacteria by en ...
... conjugated to rhodamine or Cy2 were purchased from Jackson Immunological and diluted 1 : 200. Fixable dextran 70 000 (molecular mass) labelled with Texas red (TR-dextran) at 25 mg ml21 (Molecular Probes) was used in two types of assays: (i) TR-dextran was used to analyse the uptake of bacteria by en ...
-Independent Manner Nucleus in a Cytokine
... not structurally related to the IFN receptor, the data indicated that all members of the cytokine receptor superfamily use Jaks and Stats (2). Furthermore, it is now well known that receptor tyrosine kinases such as epidermal growth factor or insulin-like growth factor activate the Jak and Stat path ...
... not structurally related to the IFN receptor, the data indicated that all members of the cytokine receptor superfamily use Jaks and Stats (2). Furthermore, it is now well known that receptor tyrosine kinases such as epidermal growth factor or insulin-like growth factor activate the Jak and Stat path ...
Cyanidioschyzon merolae Genome. A Tool for
... Figure 1. The basic courses of mitosis in a typical eukaryotic cell and unicellular red alga (C. merolae) cell. As typical cells contain many double membrane- and single membrane-bound organelles, they are not illustrated. During interphase, the centrosome (CEN) forms outside the nucleus (N), inclu ...
... Figure 1. The basic courses of mitosis in a typical eukaryotic cell and unicellular red alga (C. merolae) cell. As typical cells contain many double membrane- and single membrane-bound organelles, they are not illustrated. During interphase, the centrosome (CEN) forms outside the nucleus (N), inclu ...
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... We designed primers corresponding to sequences conserved between each fly gene and its vertebrate homologs, and performed degenerate PCR, using genomic DNA from the leech H. robusta as template, to identify leech homologs of these genes. The dorsal primers amplified a 525-bp PCR fragment encoding 17 ...
... We designed primers corresponding to sequences conserved between each fly gene and its vertebrate homologs, and performed degenerate PCR, using genomic DNA from the leech H. robusta as template, to identify leech homologs of these genes. The dorsal primers amplified a 525-bp PCR fragment encoding 17 ...
Roles of ARFRP1 (ADP-ribosylation factor
... membrane profiles on one side of the Golgi where the GFPCI-MPR-positive structures and clathrin-coated vesicles (arrowheads) were found. This result indicates that ARFRP1 is localized on the trans side of the Golgi complex and the TGN. In immunofluorescence analyses, although the anti-ARFRP1 antibod ...
... membrane profiles on one side of the Golgi where the GFPCI-MPR-positive structures and clathrin-coated vesicles (arrowheads) were found. This result indicates that ARFRP1 is localized on the trans side of the Golgi complex and the TGN. In immunofluorescence analyses, although the anti-ARFRP1 antibod ...
CD44 Mediated Endocytosis of Hyaluronan by Chondrocytes
... metacarpophalangeal joints of 18-24 month old adult bovine steers and grown as high-density monolayers (2.0 x 106 cells/ 35mm dish) in a 1:1 mixture of DMEM/Ham’sF12 medium containing 10% FBS and incubated at 37ºC in an atmosphere containing 5% CO2 [2]. Immunofluorescene microscopy: Chondrocytes wer ...
... metacarpophalangeal joints of 18-24 month old adult bovine steers and grown as high-density monolayers (2.0 x 106 cells/ 35mm dish) in a 1:1 mixture of DMEM/Ham’sF12 medium containing 10% FBS and incubated at 37ºC in an atmosphere containing 5% CO2 [2]. Immunofluorescene microscopy: Chondrocytes wer ...
Fanconi anemia genes act to suppress a cross-linker-inducible p53-
... of apoptosis (-60%), indicating that this high concentration no longer distinguishes a FA from a non-FA phenotype. In addition, MMC treatment appeared to induce a small population of polyploid cells (>G2) in HSCS36P cells, whereas in FAC-corrected cells, whichhadan elevated background level of polyp ...
... of apoptosis (-60%), indicating that this high concentration no longer distinguishes a FA from a non-FA phenotype. In addition, MMC treatment appeared to induce a small population of polyploid cells (>G2) in HSCS36P cells, whereas in FAC-corrected cells, whichhadan elevated background level of polyp ...
Motion of red blood cells in a capillary with an - AJP
... points fixed in the cell membrane. The system of differential equations is solved for dr/dt, dz/dt, p, and ts by a finite difference method; then the cell shape at the next time step is computed. To improve the stability, a partially implicit approach is used. At each time step, membrane curvature ( ...
... points fixed in the cell membrane. The system of differential equations is solved for dr/dt, dz/dt, p, and ts by a finite difference method; then the cell shape at the next time step is computed. To improve the stability, a partially implicit approach is used. At each time step, membrane curvature ( ...
Surface Charge Distribution on the Endothelial Cell of Liver Sinusoids
... used in our experiments, the hepatocytes did not bind or internalize any of the anionic markers . The luminal front of the hepatocyte plasma membrane appeared to expose only negatively charged sites that bound cationized ferritin which is internalized via coated vesicles. Both anionic and cationic m ...
... used in our experiments, the hepatocytes did not bind or internalize any of the anionic markers . The luminal front of the hepatocyte plasma membrane appeared to expose only negatively charged sites that bound cationized ferritin which is internalized via coated vesicles. Both anionic and cationic m ...
Mitosis
Mitosis is a part of the cell cycle in which chromosomes in a cell nucleus are separated into two identical sets of chromosomes, each in its own nucleus. In general, mitosis (division of the nucleus) is often followed by cytokinesis, which divides the cytoplasm, organelles and cell membrane into two new cells containing roughly equal shares of these cellular components. Mitosis and cytokinesis together define the mitotic (M) phase of an animal cell cycle—the division of the mother cell into two daughter cells, genetically identical to each other and to their parent cell.The process of mitosis is divided into stages corresponding to the completion of one set of activities and the start of the next. These stages are prophase, prometaphase, metaphase, anaphase, and telophase. During mitosis, the chromosomes, which have already duplicated, condense and attach to fibers that pull one copy of each chromosome to opposite sides of the cell. The result is two genetically identical daughter nuclei. The cell may then divide by cytokinesis to produce two daughter cells. Producing three or more daughter cells instead of normal two is a mitotic error called tripolar mitosis or multipolar mitosis (direct cell triplication / multiplication). Other errors during mitosis can induce apoptosis (programmed cell death) or cause mutations. Certain types of cancer can arise from such mutations.Mitosis occurs only in eukaryotic cells and the process varies in different organisms. For example, animals undergo an ""open"" mitosis, where the nuclear envelope breaks down before the chromosomes separate, while fungi undergo a ""closed"" mitosis, where chromosomes divide within an intact cell nucleus. Furthermore, most animal cells undergo a shape change, known as mitotic cell rounding, to adopt a near spherical morphology at the start of mitosis. Prokaryotic cells, which lack a nucleus, divide by a different process called binary fission.