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lec4-5-biosynthesis_specificity
lec4-5-biosynthesis_specificity

... • a good 3-D structure should be available • It is often based on molecular graphics or more advanced molecular modeling (structure-based computational design techniques) • Such models place an emphasis on enthalpic interactions and omit entropic contributions ...
Enzymes - WordPress.com
Enzymes - WordPress.com

... • Temperature – increases rate due to increased collisions then after optimum the enzyme structure breaks down • pH – Either side of optimum pH the H+ and OH- ions interrupt the tertiary structure and this changes the active site • Concentration of enzyme and substrate – Both increase until a platea ...
Answer Set 2
Answer Set 2

... forward reactions does not take place to a significant extent. Under intracellular conditions, ΔG is -1.3 kJ/mol. Using the equation ΔG = ΔGo’ + RT ln [products]/[reactants] and solving for [products]/[reactants] gives a ratio of 3.7 x 10-5. Thus, a reaction that is endergonic under standard conditi ...
1. A Draw the structure of glucose using either a ring or straight
1. A Draw the structure of glucose using either a ring or straight

... C. Ferredoxin (Fd) is an iron containing protein present in the photosynthetic apparatus; during photosynthesis the iron undergoes a 1-electron redox reaction with an Eo' = -0.42v. Similarly cytochrome b5 is an iron-protein present in the microsomes with an Eo' = 0.0 v. For the reaction at pH = 7 ...
enzyme - Clayton State University
enzyme - Clayton State University

... • External signals can regulate the activity of enzymes (e.g. neurotransmitters or hormones) • Chemical messenger initiates a signal cascade which activates enzymes called protein kinases • Protein kinases phosphorylate target enzymes to affect activity Example ...
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File

... carboxylase/oxygenase (rubisco) catalyzes the reaction.  Rubisco is a slug, relatively speaking ...
free energy - Thunderbird High School
free energy - Thunderbird High School

... ones • The synthesis of protein from amino acids is an example of anabolism • Bioenergetics is the study of how organisms manage their energy resources In your own words describe the difference between catabolic pathways and anabolic pathways. ...
Fatty Acid Degradation Catabolism Overview TAG and FA
Fatty Acid Degradation Catabolism Overview TAG and FA

... • Odd chain fatty acids – Rare, but do occur in diet – One of 2 requirements for Vitamin B12 (cobalamine) in human diet ...
Acid-Base Catalysis
Acid-Base Catalysis

... reaction to occur, the substrate most come into close proximity to catalytic functional groups (side chains of amino acids which are involved in catalytic reactions) with in the active site. In addition, the substrate must be precisely oriented to the catalytic groups. Once the substrate is correctl ...
Chapter Nine - The Krebs Cycle
Chapter Nine - The Krebs Cycle

... • Hans Kreb discovered its cyclic nature • Goes by three names – Citric acid cycle – Tricarboxylic cycle – Krebs cycle ...
University of - Biochemistry at the University of Maryland, College Park
University of - Biochemistry at the University of Maryland, College Park

... (a; 12 points) Listed below are three enzymes from the glycolysis pathway. For any two (your choice) of these enzymes, draw the complete structure (including all H atoms) of the reactants and products for the reaction catalyzed by that enzyme, and write the names of the reactants and products. You m ...
SI Worksheet 7
SI Worksheet 7

... 6. The ultimate source of the energy the enables cellular respiration to occur is a. glucose b. fermentation c. oxygen d. biosynthesis e. the sun 7. Unlike turkey breast, the breast of duck is “dark meat.” Why? a. ducks fly longer distances, so their breast muscles consist of fast fibers b. ducks fl ...
FACTORS AFFECTING ENZYME ACTION
FACTORS AFFECTING ENZYME ACTION

... green line. The lower energy allows the reaction to happen inside the body/organism at a lower temperature and at a faster rate. HOW USEFUL! Tip – without enzymes we would not be able to function properly. ...
ENZYME STRUCTURE AND FUNCTION
ENZYME STRUCTURE AND FUNCTION

... very difficult problem that has not yet been solved. Most enzymes are much larger than the substrates they act on, and only a small portion of the enzyme (around 2–4 amino acids) is directly involved in catalysis. The region that contains these catalytic residues, binds the substrate, and then carri ...
Photosynthesis Chloroplasts Light Reactions (photons → NADPH +
Photosynthesis Chloroplasts Light Reactions (photons → NADPH +

... Internal conversion - electronic energy converted to heat, time frame <10-11 s Fluorescence - excited state decays to ground state by emitting photon, time frame ~10-8 s Exciton transfer (resonance energy transfer) - excited molecule transfers its excitation energy to nearby unexcited molecules, imp ...
Biochemistry I, Spring Term 2000 - Third Exam
Biochemistry I, Spring Term 2000 - Third Exam

... A mutant lysozyme was made such that Asp53 was replaced with Asn. Would this enzyme still be active? Why? Do you expect the activity to be the same, decrease, or increase? Justify your answer. Asp53 stabilizes the carbocation. It is not essential for activity, but replacement of it with Asn would re ...
Fatty Acid Catabolism
Fatty Acid Catabolism

... 3. The conversion of the laurate CoA (a 12‐carbon acylCoA) to carbon dioxide via β‐ oxidation, the citric acid cycle and oxidative phosphorylation yield approximately  ________ ATP equivalents.  A) 78  B) 80  C) 82  D) 84 4. A patient is found to have a high concentration of cholesterol in the blood ...
Chem*3560 Lecture 16: Reciprocal regulation of glycolysis and
Chem*3560 Lecture 16: Reciprocal regulation of glycolysis and

... Although pyruvate dehydrogenase does not lie in either the glycolysis or gluconeogenesis sequences, its activity could divert pyruvate into the TCA cycle. Pyruvate dehydrogenase is regulated by product inhibition as well as by phosphorylation, which decrease activity. Product inhibition occurs when ...
Patrick_Chapter_4
Patrick_Chapter_4

... Acts as a basic catalyst (proton 'sink') ...
Chapter 6-Photosynthesis
Chapter 6-Photosynthesis

... (1) Without a concentration gradient, there would be no tendency for protons to move from the thylakoid into the stroma. As a result, ATP would not be made by ATP synthase. Also, there would be fewer protons in the stroma to combine with NADP and make NADPH. (2) Increasing the carbon dioxide concent ...
Citric Acid Cycle Overview
Citric Acid Cycle Overview

... Dihydrolipoamide Acyltransferase (E2) • Transfer catalyzed  by E1 • Serves as an linker  to “swing”  substrate through  subunits • Mechanism of  redox ...
Structures and mechanisms
Structures and mechanisms

... activities of enzymes are determined by their three-dimensional structure.[20] However, although structure does determine function, predicting a novel enzyme's activity just from its structure is a very difficult problem that has not yet been solved.[21] Most enzymes are much larger than the substra ...
Topic 3 Proteins as Drug Targets
Topic 3 Proteins as Drug Targets

... EE ...
ch3b_SP13x
ch3b_SP13x

... – NAD+ regenerated by electron transport chain (oxidative phosphorylation) ...
Rational design_substrate specificity
Rational design_substrate specificity

... Nitrous Acid or NH3 +CO2 ...
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Luciferase



Luciferase is a generic term for the class of oxidative enzymes used in bioluminescence and is distinct from a photoprotein. The name is derived from Lucifer, the root of which means 'light-bearer' (lucem ferre). One example is the firefly luciferase (EC 1.13.12.7) from the firefly Photinus pyralis. ""Firefly luciferase"" as a laboratory reagent often refers to P. pyralis luciferase although recombinant luciferases from several other species of fireflies are also commercially available.
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