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the active site
the active site

... EE ...
1MBO Lopez kin
1MBO Lopez kin

... release free iron and is not utilized as a source of cellular heme3. While the traditional textbook roles for heme as a cofactor include hemo- and myoglobins, cytochromes and a handful of enzymes, considerable evidence has emerged that demonstrates a central role for heme in regulation of gene trans ...
enzyme
enzyme

... EE ...
Answer: ( c ) Relative specificity One of the main characteristics
Answer: ( c ) Relative specificity One of the main characteristics

... One of the main characteristics of enzymes is their high specificity. Enzymes are specific for: a) the substrate b) the reaction It means that they catalyze the transformation of just one substrate or a family of substrates that are structurally related, catalyzing only one of the possible reactions ...
TriStar² LB 942 - Berthold Technologies USA
TriStar² LB 942 - Berthold Technologies USA

... format with multiple selection options as well as the choice of an individual directory per measurement protocol. ...
lec27_2013 - Andrew.cmu.edu
lec27_2013 - Andrew.cmu.edu

... A. Phosphatase: Removes a phosphate group from a substrate, no ATP/ADP required. A phosphatase is distinct from an ATPase. B. Kinase: transfers a phosphate group from ATP to another compound (i.e. hexokinase, galactose kinase, pyruvate kinase). A kinase is distinct from an ATPase C. Mutase: Common u ...
Review session for exam-I
Review session for exam-I

... enzymes. resembles the transition-state structure of the normal enzyme-substrate complex. stabilizes the transition state for the normal enzyme-substrate complex. typically reacts more rapidly with an enzyme than the normal substrate. ...
22. Think of two different proteins: both are enzymes. a) What
22. Think of two different proteins: both are enzymes. a) What

... Phosphorylation/Dephosphorylation: requires donor of phosphate group (ATP=P1+energy) d) What is the main difference between the mechanism in c) and allosteric control? Two enzymes are needed to regulate for covalent binding in phosphorylation/de. and no enzymes are used for non covalent allosteric b ...
Protein enzyme
Protein enzyme

... Acts as a basic catalyst (proton 'sink') ...
Biochem 330 Fall 2011 Problem Set II Enzyme Catalysis, Glycolysis
Biochem 330 Fall 2011 Problem Set II Enzyme Catalysis, Glycolysis

... 1. a) The reactions catalyzed by the ten enzymes of glycolysis can be chemically classified into the five following groups. What is the general name for an enzyme which catalyzes this kind of chemical reaction and which enzymes of glycolysis fall into these categories. Answers for the first group ha ...
ch3a FA11 - Cal State LA
ch3a FA11 - Cal State LA

... • Mechanism: form an Enzyme-Substrate (ES) complex at active site – Enhance substrate reactivity • Enhance polarity of bonds via interaction with amino acid functional groups • Possibly form covalent bonded intermediates with amino acid side chains ...
PHOTOSYNTHESIS - Green Local Schools
PHOTOSYNTHESIS - Green Local Schools

... 6CO2+6H2O+ energy(ATP)  The complex process in which cells make ATP by breaking down organic compounds  Heterotrophs – Organisms that obtain energy from eating autotrophs or other heterotrophs ...
Document
Document

... substrate but cannot be converted to products ...
Artificial Photosynthesis - The Mars Homestead Project
Artificial Photosynthesis - The Mars Homestead Project

... Calcium Magnesium Potassium Sodium Iron Phosphorus ...
What enzymes do do!! Increase the rate of a reaction by lowering the
What enzymes do do!! Increase the rate of a reaction by lowering the

... Increase the rate of a reaction by lowering the activation energy ∆G# Reaction specificity and the activation energy of a reaction Enzymes have evolved special active site pockets which recognise only certain substrates and allow them to bind. This makes enzymes generally more specific than non-prot ...
1 Lecture 27: Metabolic Pathways Part I: Glycolysis
1 Lecture 27: Metabolic Pathways Part I: Glycolysis

... This completes the “first stage” of glycolysis. Overall Δ G for the first 5 steps under cellular conditions is -53 kJ/mol. So far, 2 ATP molecules have been consumed. ...
Sample Question Set 5a
Sample Question Set 5a

... maximum number of ATPs that can be synthesized, assuming standard conditions and 100% conservation of energy? (ΔG°’ = 30.5 kJ mol-1 for ATP synthesis) 18. When the F1 prtion of the ATP synthase complex is removed from the mitochondrial membrane and studied in solution, it functions as an ATPase, i.e ...
PHY3072 - MUSCLE AND EXERCISE LECTURE 2: Introduction to
PHY3072 - MUSCLE AND EXERCISE LECTURE 2: Introduction to

... optimum range - Structure of the enzyme may be changed - Active site is distorted and the substrate no longer fits ...
Mammalian Two-Hybrid Assay Kit
Mammalian Two-Hybrid Assay Kit

... detecting protein-protein interactions in vivo in mammalian cells. The twohybrid assay kit utilizes hybrid genes to detect protein-protein interactions via the activation of reporter-gene expression. The mammalian two-hybrid reporter plasmid, pFR-Luc (see Figure 5), contains a synthetic promoter wit ...
06_Isoenzymes. Enzymodiagnostics. Enzymopathy. Enzymotherapy
06_Isoenzymes. Enzymodiagnostics. Enzymopathy. Enzymotherapy

... enzyme is stable. The rate of most enzymatic reactions doubles for each 100 C rise in temperature. This is true only up to about 500 C. Above this temperature, we observe heat inactivation of enzymes. The optimum temperature of an enzyme is that temperature at which the greatest amount of substrate ...
1 Enzyme Mechanisms Topics: TIM, Chymotrypsin, Rate
1 Enzyme Mechanisms Topics: TIM, Chymotrypsin, Rate

... charge and was therefore expected to kill the enzyme based on the above model. As it turned out, the activity did decrease, but only from about 108 to 10 4 . Similarly, adding a methyl group to His and mutating Ser each decreased the activity but only partially. Thus, something else had to be going ...
Chapter 4
Chapter 4

... A regulatory molecule binds to a site separate from the active site (like small molecules to repressors in operons) Induced conformational changes regulate the activity of the enzyme These enzymes usually have catalytic and regulatory domains Can have multiple domains or subunits for different regul ...
Fatty Acid Catabolism
Fatty Acid Catabolism

... D) Lysophospholipid CoA. 2. There are four steps in the β-oxidation pathway. Some reaction types are listed below. Give the proper reaction types in the order that they occur in the β-oxidation pathway. ...
No Slide Title
No Slide Title

... 4) What are the reactants of cellular respiration? 5) What are the products of cellular respiration? 6) What are the three parts of an ATP molecule? 7) What is the energy content of ATP relative to that of glucose? 8) What produces the “high energy” nature of ATP? 9) What happens when a molecule bec ...
1. The graph shows the relative levels of Cdk1 and cyclin B
1. The graph shows the relative levels of Cdk1 and cyclin B

... prematurely. Otherwise, because trypsin can activate trypsinogen, there is potential for an escalating series of activations that would be catastrophic for the pancreas. When the zymogens are secreted into the duodenum, they encounter enteropeptidase, which is an membrane-anchored enzyme on the extr ...
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Luciferase



Luciferase is a generic term for the class of oxidative enzymes used in bioluminescence and is distinct from a photoprotein. The name is derived from Lucifer, the root of which means 'light-bearer' (lucem ferre). One example is the firefly luciferase (EC 1.13.12.7) from the firefly Photinus pyralis. ""Firefly luciferase"" as a laboratory reagent often refers to P. pyralis luciferase although recombinant luciferases from several other species of fireflies are also commercially available.
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