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Membrane Trafficking During Plant Cytokinesis
Membrane Trafficking During Plant Cytokinesis

... ADL1A at the cell plate during cytokinesis (Kang and Bednarek, unpublished). More recent studies have suggested a role for ADL1A in polarized vesicular trafficking and endocytosis. ADL1A and other proteins involved in vesicular transport between the TGN, plasma membrane and endocytic compartments inc ...
Cell-A-Brate - 6.LS.1 - Columbus City Schools
Cell-A-Brate - 6.LS.1 - Columbus City Schools

... these you should point out that in both there is a progression of size but in the levels of organization of living things each level is a part of the level above it working from smallest to largest. The final product can be made with random cells, etc., but higher level students should be encouraged ...
Bacterial toxins modifying the actin cytoskeleton
Bacterial toxins modifying the actin cytoskeleton

... which is closely related to ToxB (88% amino acid sequence identity and immunological cross reactions between the two toxins) and a hemorrhagic toxin (HT). C. novyi is an etiological agent of gangrene with major edema. The main toxin produced is the alpha toxin. The large clostridial toxins have a co ...
A role for the DNA-damage checkpoint kinase Chk1 in the virulence
A role for the DNA-damage checkpoint kinase Chk1 in the virulence

... program in pathogenic fungi. It could therefore be assumed that in pathogenic fungi the control of the cell cycle, as well as morphogenesis, is linked somehow to the virulence program. The maize smut fungus Ustilago maydis is an excellent system to address the relationships between cell cycle, morph ...
The Human Arp2/3 Complex Is Composed of Evolutionarily
The Human Arp2/3 Complex Is Composed of Evolutionarily

... WD repeat sequences (also known as b-transducin repeats). We identified seven potential WD repeats in p41Arc (Fig. 1 A) that correspond to the consensus sequence described by Neer et al. (1994) with one to five mismatches. These repeats are highly conserved in both sequence and position within p41-A ...
The Small GTPases Rho and Rac Are Required for the
The Small GTPases Rho and Rac Are Required for the

... and after 15 min, the coated bead suspension was incubated with the cells (105 beads/coverslip) for 45 min at 378C in 5% CO2 atmosphere. After three washes in PBS, cells were fixed in 3% paraformaldehyde, permeabilized, and labeled with phalloidin–Texas red. Controls for recruited cytoplasmic protei ...
Modes of Membrane Transport
Modes of Membrane Transport

... – 2 substances are moved in opposite directions • Some carriers passively transport substances down their respective concentration gradient • Other carriers actively transport substances up their respective concentration gradient – carriers called pumps hydrolyze a molecule of ATP and use the energy ...
Solutions
Solutions

... – 2 substances are moved in opposite directions • Some carriers passively transport substances down their respective concentration gradient • Other carriers actively transport substances up their respective concentration gradient – carriers called pumps hydrolyze a molecule of ATP and use the energy ...
Powerpoint Presentation: Transport in Plants
Powerpoint Presentation: Transport in Plants

... At any point across the root the minerals can be taken up by a cell across a plasma ...
Understanding the role of cholesterol in cellular biomechanics and
Understanding the role of cholesterol in cellular biomechanics and

... liquid ordered and less fluid phases [88,91] (reviewed by [94]). Cholesterol-enriched membrane regions, also called membrane rafts, were first identified as liquid ordered domains, containing high amounts of cholesterol and sphingomyelin and usually resistant to detergent solubilization (reason why ...
Endocytosis, Actin Cytoskeleton, and Signaling
Endocytosis, Actin Cytoskeleton, and Signaling

... were found in the Arabidopsis genome, indicating an essential role of endocytosis in plant cells. Plant Rab GTPases Ara6 and Ara7, related to the mammalian endosomal protein Rab5, as well as Pra2, related to mammalian Rab11, have been localized to endosomes or putative endosomes, respectively (Ueda ...
msc_botnay_pre_pap1_bl1 - Madhya Pradesh Bhoj Open
msc_botnay_pre_pap1_bl1 - Madhya Pradesh Bhoj Open

... reactions that take place in the cell would normally occur too slowly to maintain life as we know it. Cell requires a set of information to carry out the reactions. The extraneous coat of the plasma membrane of the plant cell is known as cell wall. The cell wall is a rigid and protective layer aroun ...
BIOSYNTHESIS IN ISOLATED ACETABULARIA CHLOROPLASTS I
BIOSYNTHESIS IN ISOLATED ACETABULARIA CHLOROPLASTS I

... clear that chloroplasts possess their own proteinsynthesizing system (17, 45). On the other hand, chloroplasts do not carry the genetic information for all of their important constituent enzymes (25, ...
Cytoskeletal Motors in Arabidopsis. Sixty
Cytoskeletal Motors in Arabidopsis. Sixty

... domains are found among most of them. Phylogenetic analysis of their motor domains has placed most of them into established kinesin subfamilies (Reddy and Day, 2001b). When an Arabidopsis kinesin and an animal or fungal one are grouped into the same subfamily, however, one should not simply interpre ...
In yeast, the pseudohyphal phenotype induced by isoamyl alcohol
In yeast, the pseudohyphal phenotype induced by isoamyl alcohol

... that operating during nitrogen-limited growth because it has a distinct set of genetic requirements. For example, butanolinduced pseudohyphal formation is dependent on SWE1 (La Valle and Wittenberg, 2001) but is independent of genes such as FLO8 and FLO11 that are required for pseudohyphal induction ...
Chloroplast The chloroplast is an elongated organelle
Chloroplast The chloroplast is an elongated organelle

... The energy used to convert water plus atmospheric carbon dioxide into sugars (photosynthesis) occurs in the c______. The outer and inner membranes, which regulate the flow of sugars and proteins in and out of the chloroplast, are called the c______ e______. The inside of the chloroplast has fluid ca ...
The Cell - University of South Carolina
The Cell - University of South Carolina

... A mitochondrion (plural mitochondria) is a membraneenclosed organelle found in most eukaryotic cells. These organelles range from 0.5 to 10 micrometers (µm) in diameter. Mitochondria are sometimes described as "cellular power plants" because they generate most of the cell's supply of adenosine triph ...
Organelle Project - WLPCS Upper School
Organelle Project - WLPCS Upper School

... Organelle Frayer Model Directions: Create a Frayer model of a cell organelle. Use past work and Sec 7.3 in textbook as resources A colored, labeled drawing of the organelle and description of the structure general location in the cell. (Ex. The nucleus is generally in the ...
Jamming prokaryotic cell-to-cell communications in a model biofilm†
Jamming prokaryotic cell-to-cell communications in a model biofilm†

... bacterial reproduction, we allow b to vary with time according to bg2t/v, where n is the rate constant for doubling. Using eqn (1) we simulated and fit the GFP-LVA expression measured by the fluorescence data to extract parameters that described GFP production and degradation, and the C6-HSL concent ...
Sample pages 1 PDF
Sample pages 1 PDF

... The Arabidopsis genome has 54 SNARE genes (18 Qa-SNAREs/Syntaxins, 11 Qb-SNAREs, 8 Qc-SNAREs, 14 R-SNAREs/VAMPs and 3 SNAP-25s) (Uemura et al. 2004), many of which are plant-specific and those associated with the PM have roles in cytokinesis, hormone responses, and pathogen resistance (Collins et al ...
Organelle Fission. Crossing the Evolutionary Divide
Organelle Fission. Crossing the Evolutionary Divide

... that localizes to the matrix surface of the inner membrane has recently been described in Schizosaccharomyces pombe (Pelloquin et al., 1999). In chloroplasts FtsZ appears to operate on both membrane surfaces, though this does not preclude the involvement of dynamins or other cytoskeletal proteins as ...
Signaling tip growth in plants Zhenbiao Yang
Signaling tip growth in plants Zhenbiao Yang

... growth in root hairs, therefore, will not only reveal tip growth mechanisms but also shed light on other fundamental cellular processes. ...
7-2 Eukaryotic Cell Structure
7-2 Eukaryotic Cell Structure

... proteins into small molecules that can be used by the rest of the cell. Lysosomes also break down organelles that have outlived their usefulness. Slide 26 of 49 Copyright Pearson Prentice Hall ...
7-2 Eukaryotic Cell Structure
7-2 Eukaryotic Cell Structure

... proteins into small molecules that can be used by the rest of the cell. Lysosomes also break down organelles that have outlived their usefulness. Slide 26 of 49 Copyright Pearson Prentice Hall ...
7-2 Eukaryotic Cell Structure
7-2 Eukaryotic Cell Structure

... proteins into small molecules that can be used by the rest of the cell. Lysosomes also break down organelles that have outlived their usefulness. Slide 26 of 49 Copyright Pearson Prentice Hall ...
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Cytoplasmic streaming



Cytoplasmic streaming, also called protoplasmic streaming and cyclosis, is the directed flow of cytosol (the liquid component of the cytoplasm) and organelles around large fungal and plant cells through the mediation of actin. This movement aids in the delivery of organelles, nutrients, metabolites, genetic information, and other materials to all parts of the cell. Cytoplasmic streaming occurs along actin filaments in the cytoskeleton of the cell.Cytoplasmic streaming was first discovered in the 1830s. The scientific breakthrough assisted scientists in developing an understanding of the different roles of cells and how they function as the basic operating systems of life.This process occurs through the operation of motor proteins called myosins.These proteins use energy of adenosine triphosphate (ATP) to act as a molecular motor, which slides along actin filaments. This works in a manner that tows the organelles and other cytoplasmic contents in the same direction. Myosin proteins consist of two conjoined proteins. If one protein remains attached to the substrate, the substance acted upon by the protein, such as a microfilament, has the ability to move organelles through the cytoplasm.The green alga genus Chara and other genera in the Division Charophyta, such as Coleochaete, are thought to be the closest relatives of land plants. These haploid organisms contain some of the largest plant cells on earth, a single cell of which can reach up to 10 cm in length. The large size of these cells demands an efficient means to distribute resources, which is enabled via cytoplasmic streaming.Cytoplasmic streaming is strongly dependent upon intracellular pH and temperature. It has been observed that the effect of temperature on cytoplasmic streaming created linear variance and dependence at different high temperatures in comparison to low temperatures. This process is complicated, with temperature alterations in the system increasing its efficiency, with other factors such as the transport of ions across the membrane being simultaneously affected. This is due to cells homeostasis depending upon active transport which may be affected at some critical temperatures.In plant cells, chloroplasts may be moved around with the stream, possibly to a position of optimum light absorption for photosynthesis. The rate of motion is usually affected by light exposure, temperature, and pH levels.In reference to pH, because actin and myosin are both proteins, strong dependence on pH is expected. The optimal pH at which cytoplasmic streaming is highest, is achieved at neutral pH and decreases at both low and high pH.The flow of cytoplasm may be stopped by:Adding Lugol's iodine solutionAdding Cytochalasin D (dissolved in dimethyl sulfoxide)↑ ↑ ↑ ↑ ↑ ↑
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