Department of Microbiology, Lab 016 instructions Standard gel
... Standard gel electrophoresis of nucleic acids or PCR products – for other applications with other agarose types, check specific literature Reagents / equipment: Standard agarose 1-1,5 %. Dissolve 1-1,5 gram in 100 ml 1xTAE buffer (diluted with Aqua dest from a 100 x stock solution). Heat in micro ov ...
... Standard gel electrophoresis of nucleic acids or PCR products – for other applications with other agarose types, check specific literature Reagents / equipment: Standard agarose 1-1,5 %. Dissolve 1-1,5 gram in 100 ml 1xTAE buffer (diluted with Aqua dest from a 100 x stock solution). Heat in micro ov ...
CANADIAN TRANSLATION OF FISHERIES AND AQUATIC
... a cathodal position in relation to the serum deposit among all males examined, or an anodal position 2mong females. ...
... a cathodal position in relation to the serum deposit among all males examined, or an anodal position 2mong females. ...
Prezentace aplikace PowerPoint
... 1. It is the process of moving charged biomolecules in solution by applying an electrical field across the mixture. 2. Biomolecules moved with a speed dependent on their charge, shape, and size and separation occures on the basis of molecular size. Electrophoresis is used: for analysis and purificat ...
... 1. It is the process of moving charged biomolecules in solution by applying an electrical field across the mixture. 2. Biomolecules moved with a speed dependent on their charge, shape, and size and separation occures on the basis of molecular size. Electrophoresis is used: for analysis and purificat ...
100bp DNA Ladder RTU (Ready-to-Use) Cat. No. MWD100 Size
... A unique combination of PCR products and a number of proprietary plasmids digested with appropriate restriction enzymes to yield 12 fragments, suitable for use as molecular weight standards for agarose gel electrophoresis. The DNA includes fragments ranging from 100-3,000 base pairs. The 500 and 1,5 ...
... A unique combination of PCR products and a number of proprietary plasmids digested with appropriate restriction enzymes to yield 12 fragments, suitable for use as molecular weight standards for agarose gel electrophoresis. The DNA includes fragments ranging from 100-3,000 base pairs. The 500 and 1,5 ...
Document
... • Sodium Dodecyl Sulfate denatures protein and covers it with negative charges : moves to + end • Vertical gels are designed so the top of the gel box is attached to the negative power outlet • The bottom of the gel box is attached to the ...
... • Sodium Dodecyl Sulfate denatures protein and covers it with negative charges : moves to + end • Vertical gels are designed so the top of the gel box is attached to the negative power outlet • The bottom of the gel box is attached to the ...
Bacterial Evolution: Sample Risk Assessment
... Activity closely supervised Technician to prepare agarose and handle concentrated stain solution Tips and gloves disposed of in chemical waste Students told to avoid touching gels Students to wear gloves when removing combs from gels and removing gel trays from tanks for visualisation De ...
... Activity closely supervised Technician to prepare agarose and handle concentrated stain solution Tips and gloves disposed of in chemical waste Students told to avoid touching gels Students to wear gloves when removing combs from gels and removing gel trays from tanks for visualisation De ...
Electrophoresis
... In CE, the classic techniques of electrophoresis are carried out in a small-bore, fused silica capillary tube, the outer diameter of such tubes typically varies from 180 to 375 micrometer, the inner diameter from 20 to 180 micrometer, and the total length from 20 cm up to several meters. This capill ...
... In CE, the classic techniques of electrophoresis are carried out in a small-bore, fused silica capillary tube, the outer diameter of such tubes typically varies from 180 to 375 micrometer, the inner diameter from 20 to 180 micrometer, and the total length from 20 cm up to several meters. This capill ...
Gel electrophoresis
Gel electrophoresis is a method for separation and analysis of macromolecules (DNA, RNA and proteins) and their fragments, based on their size and charge. It is used in clinical chemistry to separate proteins by charge and/or size (IEF agarose, essentially size independent) and in biochemistry and molecular biology to separate a mixed population of DNA and RNA fragments by length, to estimate the size of DNA and RNA fragments or to separate proteins by charge.Nucleic acid molecules are separated by applying an electric field to move the negatively charged molecules through a matrix of agarose or other substances. Shorter molecules move faster and migrate farther than longer ones because shorter molecules migrate more easily through the pores of the gel. This phenomenon is called sieving.Proteins are separated by charge in agarose because the pores of the gel are too large to sieve proteins. Gel electrophoresis can also be used for separation of nanoparticles.Gel electrophoresis uses a gel as an anticonvective medium and/or sieving medium during electrophoresis, the movement of a charged particle in an electrical field. Gels suppress the thermal convection caused by application of the electric field, and can also act as a sieving medium, retarding the passage of molecules; gels can also simply serve to maintain the finished separation, so that a post electrophoresis stain can be applied. DNA Gel electrophoresis is usually performed for analytical purposes, often after amplification of DNA via PCR, but may be used as a preparative technique prior to use of other methods such as mass spectrometry, RFLP, PCR, cloning, DNA sequencing, or Southern blotting for further characterization.