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Chapter 5
Chapter 5

... DNA sequence and cleave at specific DNA sequence (scalpels or scissors); •  Discovered by Werner Arber and Hamilton Smith and first used by Daniel Nathans; •  Usually recognize palindromic sequences. ...
BIOL 221-GENETICS
BIOL 221-GENETICS

... II. Genes on Chromosomes A. Evidence for genes on chromosomes 1. history of the question 2. white-eyed flies and sex determination B. Chromosomal results of mitosis and meiosis 1. events of mitosis and meiosis 2. meiosis and Mendelism C. Linkage and recombination 1. crossing over 2. determining link ...
Intro to Strawberry DNA Extraction Lab
Intro to Strawberry DNA Extraction Lab

... Where have you heard of genes before? What do genes have to do with DNA? Gene = Segments of DNA that control the production of protein ...
KS3 Science
KS3 Science

... e Do you think there is a correlation (relationship) between the size of an organism and the number of chromosomes in its body cells? ...
Prediction practice - unlinked
Prediction practice - unlinked

... • Samples are collected and then broken into fragments using specific enzymes (restriction endonucleases) before being separated using gel electrophoresis. This produces a banding pattern unique to an individual. ...
Vocabulary Quiz Key Terms
Vocabulary Quiz Key Terms

... An enzyme that breaks the hydrogen bonds holding the base pairs together as it unwinds and unzips the double helix, allowing new nucleotides to bind to the 2 single strands by base pairing. An enzyme that adds complementary nucleotides to the template strand of the unzipped double helix until the en ...
Biotechnology
Biotechnology

... DNA is universal language and code for the same amino acids ...
DNA functions worksheet
DNA functions worksheet

... 2. What is the main difference between the structure of chromatin and the structure of chromosomes? ...
understanding dna molecule of heredity - Cal State LA
understanding dna molecule of heredity - Cal State LA

... The nucleotide is held together by a backbone made of sugars and phosphate group The backbone carries four types of molecules called bases It is the sequence of these four bases that encodes information The main job of the DNA is to encode the sequence of amino acids residues ...
Osteogenesis Imperfecta (OI)
Osteogenesis Imperfecta (OI)

Slide 1
Slide 1

... a new yeast cell. • This is by mitosis. ...
Regulation and Expression of Aldehyde Dehydrogenase in Normal
Regulation and Expression of Aldehyde Dehydrogenase in Normal

... Aberrant changes of DNA methylation, histone modification and chromatin compartments are commonly associated with the progression of human cancers. Hypermethylation of CpG islands is the most well categorised epigenetic change to occur in tumours. Many CpG islands associated with transcription of a ...
DNA - TeacherWeb
DNA - TeacherWeb

... the order in which the amino acids bond b. Ribosomal RNA makes up Ribosomes, where proteins are built c. Transfer RNA brings amino acids to the ribosomes to build the protein. 3. Cells use only the genes that directs the making of proteins needed by that cell. ...
File
File

The modern synthesis
The modern synthesis

S-strain (virulent)
S-strain (virulent)

... They are smaller and have only one ring. ...
TIP Translation - dna
TIP Translation - dna

... Name: _____________________ Date: ____________ Class:_________ DNA Translation Quiz Multiple Choice Identify the choice that best completes the statement or answers the question. ____ 1. What materials make up each nucleotide in a DNA molecule? a. amino acid, base, and protein c. mRNA, tRNA, and a r ...
Mutation and Recombination
Mutation and Recombination

... Natural mutation is a very rare event and can only be studied in organisms with very high division rates (such as bacteria). A spontaneous mutation may be detected by using selective media. For example, the antibiotic penicillin, could be added to the culture media. If some of the aseptically transf ...
Exam Review 2B -- Rodermel
Exam Review 2B -- Rodermel

... secondary structures from forming Unwinds DNA at replication fork ...
1 Genetics (BIL-250) Review Questions #1 (2
1 Genetics (BIL-250) Review Questions #1 (2

... major components: (1) 5’ and 3’ ends of each strand, (2) leading strand, (3) lagging strand, (4) single-stranded binding proteins, (5) DNA polymerase, (6)Okazaki fragments, (7) RNA primer, (8) DNA helicase, (9) DNA ligase, (10) primase. (3-2) How did the Meselson-Stahl experiment demonstrate that DN ...
DNA Structure Worksheet
DNA Structure Worksheet

... _______________________ ring(s) in their structure, and pyrimidines have _______________________ ring(s) in their structure. 6. The two bases that are purines are _____________________ and ________________________. These bases are comprised of ___________ rings. 7. The two bases that are pyrimidines ...
Prostate cancer stem cells Ongoing Projects 3
Prostate cancer stem cells Ongoing Projects 3

... Ini-al
therapies
for
prostate
can
be
successful
at
shrinking
the
tumour.
However,
in
 some
cases
a
secondary
tumour
emerges
and
this
is
typically
resistant
to
therapy.
 We
 hypothesise
 that
 prostate
 cancer
 stem
 cells
 are
 resistant
 to
 radia-on
 and
 chemotherapy
and
are
responsible
for
secon ...
Ch 13 Genetic Engineering
Ch 13 Genetic Engineering

... • Scientists can synthesize a DNA strand and connect it to a circular DNA molecule known as a plasmid… which can be found naturally in bacteria. This bacteria can then be injected into a plant, and will insert its DNA into the plant. • If transformation is successful, the recombinant DNA is integra ...
What is RNA? - Manhasset Schools
What is RNA? - Manhasset Schools

... DNA is too ________________ to leave the nucleus, so a smaller molecule called __________ is made to carry the _______________________ out of the _________________ so ____________________ can be made. * This is completed through the process of _________________________________ * ...
Chapter 9 Biotechnology
Chapter 9 Biotechnology

... Chapter 9 Genetic engineering ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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