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DNA TRIPLEX Triplex structures are characterized by a single
DNA TRIPLEX Triplex structures are characterized by a single

... Triplex structures are characterized by a single polynucleotide strand residing in the former major groove of a homopurine-homopyrimidine duplex , which are reviewed in Chapter 1 of this book. Two triplex motifs are known. The parallel- or pyrimidine-motif (Py) has a C- or T-rich third strand bound ...
Themes in the Development of DNA Science
Themes in the Development of DNA Science

... 1) Most of the phage DNA remains with the bacterial cells. 2) Most of the phage protein is found in the supernate fluid. 3) Most of the initially infected bacteria (in the cell pellet) remain competent to produce phage. 4) If thew mechanical stirring is omitted, both protein and DNA sdediment with t ...
Lecture #7 Date - Woodland Hills School District
Lecture #7 Date - Woodland Hills School District

... Mendel: modes of heredity in pea plants Morgan: genes located on chromosomes Griffith: bacterial work; transformation: change in genotype and phenotype due to assimilation of external substance (DNA) by a cell Avery: transformation agent was DNA ...
DNA as Videotape: Introductory Fact Sheet
DNA as Videotape: Introductory Fact Sheet

... • DNA can be edited--for example, we can take DNA containing one gene from an animal (for example, the gene for insulin from humans) and splice it biologically into the DNA of a bacterium. • That bacterium can multiply, and its offspring will contain the insulin gene. • Those bacteria can make the i ...
Bulletin 1 - DNA: The Cookbook of Life - ctahr
Bulletin 1 - DNA: The Cookbook of Life - ctahr

... human cell, 23 pairs of chromosomes fit in a structure that is one-tenth the width of a human hair, but if you unwound the chromosomes, the DNA would be six feet long. All living things contain DNA recipes and use them to make proteins. This amazing commonality across all forms of life has made poss ...
Genetic Technology
Genetic Technology

... • Genetic engineering – a faster and more reliable method for increasing the frequency of a specific allele in a population. ...
15.2 Recombinant DNA
15.2 Recombinant DNA

... because they prepare, or prime, a place for DNA polymerase to ...
Microbial Genetics
Microbial Genetics

... Bidirectional replication animation Rolling circle animation Replication always starts at new 5’ end ...
DNA Technology
DNA Technology

Ch 16-17 High
Ch 16-17 High

... •DNA Polymerase •Ligase •Nuclease and DNA Polymerase (both are repair enzymes) ...
Polymerase Chain Reaction
Polymerase Chain Reaction

... Dr. Lalani Yatawara Department of MLS, FAHS ...
16.1 * Producing DNA Fragments
16.1 * Producing DNA Fragments

... called (2). This enzyme initially forms a single strand of DNA called (3) DNA. To form the other strand requires an enzyme called (4). Another method of producing DNA fragments is to use enzymes called (5), which cut up DNA. Some of these leave fragments with straight edges, called (6) ends. Others ...
Statistical Applications in Biology and Genetics
Statistical Applications in Biology and Genetics

Human Genetic Variation - Mediapolis Community School
Human Genetic Variation - Mediapolis Community School

... • A gene is a functional and physical unit of heredity passed from parent to offspring. • Genes are pieces of DNA, and most genes contain information for making a specific protein. • Genes exist in 2 forms at each location on a chromosome. These are called alleles. • Alleles can be dominant or reces ...
Viruses as Pathogens in Bacterial Gene Regulation
Viruses as Pathogens in Bacterial Gene Regulation

... » a cytoplasmic bridge is established and DNA is passed from the bacterium with the F factor (F+) to the recipient (F-) – F-factor may be located in a separate circular region called a plasmid » if the plasmid reinserts itself into the DNA is it called an episome » an episome is an example of a tran ...
DNA Review
DNA Review

... If you were to split this sentence into individual three-letter words, you would probably read it like this: The sun was hot but the old man did not get his hat. This sentence represents a gene. Each letter corresponds to a nucleotide base, and each word represents a codon. What if you shifted the t ...
Biotechnology Content Review
Biotechnology Content Review

...  Medicine: Research in developing cures for diseases ...
Gene Q
Gene Q

... PCR primers were used to amplify Sperm number DNA isolated from one man's ...
Topic 4: Genetics - Peoria Public Schools
Topic 4: Genetics - Peoria Public Schools

... genes. Not only did the project strive to find the total genes but it attempted to find each gene’s location and each gene’s base sequence. 64. Benefits of the Human Genome Project include the ability to study how genes influence human development, the easier identification of genetic diseases, and ...
FlyCutTM XmaI - AP
FlyCutTM XmaI - AP

... with T4 DNA ligase at 25°C. Of these ligated fragments, more than 95% can be recut. ...
Chapter 13 – Genetic Engineering
Chapter 13 – Genetic Engineering

... in meiosis have been used to create plants that have more than two sets of chromosomes (2n). These are called polyploid plants. ...
Streptavidin is a small bacterial protein that binds
Streptavidin is a small bacterial protein that binds

... If the DNA has their own replication sequences and some gene (‘marker’) that allows the cells to survive under certain conditions, it can be maintained in the cells for many generations (as long as in the presence of such selective conditions). Moreover, DNA sequences can also be integrated into the ...
Promoter Analysis
Promoter Analysis

... Cofactors • Frequently the effect of DNA-binding proteins depends on co-factors • E.g. ER sits on the DNA but requires estrogen as a co-factor to function • Myc requires Max as a cofactor to stimulate transcription • If Max is coupled with Mad instead, the genes are repressed ...
File - Cowan Science
File - Cowan Science

... • Since DNA is cut at certain sequences, each piece is a different length and weight • Pieces that are heavier stay at the top of the gel, lighter will “run” to the bottom • Every person is genetically different, so DNA fingerprints are different ...
Recombinant DNA
Recombinant DNA

... jellyfish DNA that had been cut with REs Found fragment that bound exactly to mRNA – this was the gene ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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