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Chapter 13 - dewhozitz.net
Chapter 13 - dewhozitz.net

... Monohybrid Cross: Inheritance of 1 trait ...
Transcription
Transcription

... RNA that is wrapped with proteins to form ribosomes. Purpose Synthesis of primary protein structure ...
Cytology
Cytology

... checkpoints between phases, permit a new phase to begin only when the previous phase is complete and successful. At these checkpoints, gatekeeper molecules block or allow events, depending on their level of phosphorylation. desmosomes: (dezmoh-sohmz) Buttonlike plaques, present on two opposing cell ...
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Slide 1

... So, where were we? • The risk of getting skin cancer, particularly melanoma, can be reduced by taking precautions in the sun. • In this workshop we will focus more on the association between disease and genes. • Scientists have found three possible DNA sequences which are associated with skin cance ...
PCR Lab Notes
PCR Lab Notes

... There are 23 pairs of chromosomes which contains 30,000 to 50,000 genes. These genes only comprise about 5 % of chromosomal DNA. The other 95% is non-coding DNA. The sequence with the genes are introns, which is transcribed into RNA but in the end do not make a protein. ...
Recombinant DNA - Fulton County Schools
Recombinant DNA - Fulton County Schools

... molecules in the lab and then insert those molecules—along with the genes they carry—into living cells. – Machines known as DNA synthesizers are used to produce short pieces of DNA, up to several hundred bases in length. – These synthetic sequences can then be joined to natural sequences using DNA l ...
Study guide for Chapter3: Cell division and Chapter 4
Study guide for Chapter3: Cell division and Chapter 4

... Study guide for Unit: Chapters 3 and 4.3 test (DNA, Mitosis and Meiosis) Please use the following resources as you complete this study guide: Ttextbook pages C 73-92, C117-119 and C 135-136 1. Define the following terms: Chromosomes, Chromatids, DNA, Cell cycle, Haploid, Diploid Cell Division, Unice ...
AP Biology, Chapter 16 The Molecular Basis of Inheritance Life`s
AP Biology, Chapter 16 The Molecular Basis of Inheritance Life`s

... Telomeres shorten with each replication Contain repeated TTAGGG = extra DNA We can lose some at each replication without effect As we age the telomeres get shorter Telomerase has built-in primer, can add more TTAGGG Present in germ cell lines And present in cancer cells 16.3 A chromosome consists of ...
Manipulating DNA - Biology R: 4(A,C)
Manipulating DNA - Biology R: 4(A,C)

...  2. Look carefully at the series, and find this sequence of letters: GTTAAC. It may appear more than once. How many occurrences of the sequence GTTAAC can you find? ...
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... 3 – Sequencing (detection of mutations, paternity tests) 4 – Detection of viruses, parasites and bacteria 5 – Detection of GMOs 6 – In situ PCR (detection of given sequences in given subcellular localizations) 7 – Estimation of gene expression level ...
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REVIEW SHEET FOR GENETIC ENGINEERING AND TRANSGENICS

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ESSAY 1: CONCEPTION

... proteins and send them around the body, but the particular combinations of proteins cannot be predicted. There are also many ‘switches’ within the DNA that can be switched on by external factors. These external factors can control which proteins are made or unmade and that changes the result of the ...
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Cell Division and Fertilization

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... be combined with bacterial DNA so that they can later be inserted into a bacterial cell – The small, circular DNA molecules in bacteria (called plasmids) can be removed and cut with a restriction enzyme. – The cut ends are sticky to the foreign fragment, and can allow for the formation of a recombin ...
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... sequence of bases (A,C,T, and G). 4. Proteins A and B have the same function and the same sequence of bases (A,C,T, and G). ___ 4. Which statement best describes the result of some of the processes involved in genetic engineering? 1. They alter the arrangement of hereditary material. 2. They provide ...
Chapter 12-13 Notes
Chapter 12-13 Notes

... The tips of chromosomes are known as telomeres. The ends of DNA molecules, located at the telomeres, are particularly difficult to copy. Over time, DNA may be lost from telomeres each time a chromosome is replicated. Telomerase: 1. adds short, repeated DNA sequences to telomeres 2. lengthens the chr ...
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BIO 245: Principles of Genetics Course description BIO 245

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... 2. Next, scan the objectives for the topic you are about to study in order to get a sense of what you should be focusing your time and energy on. 3. Start mastering each objective by answering the associated review questions right on this sheet. 4. After you have finished, use this sheet as a study ...
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... 1. dephosphorylation of various proteins to allow the transition from G1 into S. 2. phosphorylation of various proteins to allow the transition from G2 into M. 3. arrest the cell at the G2/M transition until the proper signals are received. 4. dephosphorylation of various proteins to allow the trans ...
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... Originally, the DNA polymerase would have to be added between each heating step because it would fall apart at 75 degrees. Now, an enzyme called Taq DNA polymerase is added. This is a very stable enzyme isolated from bacteria living at thermal vents in the ocean (up to 95 oC) In just 32 rounds of PC ...
Bioprecursor Prodrugs
Bioprecursor Prodrugs

... TWO mechanisms of action: 1. Inhibits DNA polymerase 2. Incorporated into DNA affording incorrect base pairing and template activity ...
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PROTEIN SYNTHESIS

... With the sequencing of the human genome, molecular biologists have isolated the genes for many important genes and their resulting proteins. While most research centers on finding the genes and proteins to help cure disease (ie. Osteoporosis, cancer, diabetes, etc.), a fringe group lead by Dr. Dickm ...
MTaxonom_1
MTaxonom_1

... Here we will be most concerned with Genus species, and strains Homo genus, e.g., Species sapiens (Humans) Escherichia coli O157:H7 ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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