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DNA - Mrs. Barrett`s Biology Site
DNA - Mrs. Barrett`s Biology Site

... DNA profiling is a method of making a unique pattern of bands from the DNA of a person, which is used to distinguish that DNA from other DNA.  DNA is extracted from cells e.g. blood or semen by breaking up the cell membrane.  DNA amplification can be used if the quantity of DNA is low. Increasing ...
Transcription PPT
Transcription PPT

Chapter 7
Chapter 7

1 •Mitosis •Meiosis •Sex and Genetic Variability •Cloning
1 •Mitosis •Meiosis •Sex and Genetic Variability •Cloning

... DNA Replication & Mitosis is the same in all organisms •Both strands of DNA are used as a TEMPLATE for replication •The entire genome is faithfully replicated ...
Protein Synthesis Simulation
Protein Synthesis Simulation

... DNA carries the information for the synthesis of all the proteins of an organism. Protein molecules are large and complex, composed of hundreds of amino acids. The sequence of amino acids in a protein molecule is determined by the sequence of the nucleotides in the DNA of an organism. In the first s ...
DNA and PROTEIN SYNTHESIS
DNA and PROTEIN SYNTHESIS

... ex) If mRNA codes UUU, t RNA anticodon is AAA and it will get the amino acid PHE ...
NUCLEIC ACIDS
NUCLEIC ACIDS

... 1. Gene is composed of – exons and introns a. code for protein – exons – “expressed” in making proteins b. do not code for protein – introns 2. Both introns and exons are transcribed by mRNA -but introns are non-coding for proteins and – do not leave nucleus (may be old DNA no longer used, or may re ...
DNA, RNA and Protein Synthesis
DNA, RNA and Protein Synthesis

... Polymerase) unzips DNA 2. RNA Polymerase attaches RNA nucleotides following base pairing rules. – This makes single stranded mRNA 3. Single stranded mRNA leaves the nucleus and goes to the ribosome. ...
Yeasts
Yeasts

... Plasmid vectors can replicate with ori, an ori from one yeast strain can normally function in different yeast hosts, albeit not always with the same degree of efficiency. Up to 200 copies can be present in a single cell via additional selection. ...
Can environmental factors acting on an organism cause inherited
Can environmental factors acting on an organism cause inherited

... Epigenetics as understood in modern biology is “the study of heritable changes in gene function that occur without a change in the sequence of nuclear DNA”.1 this refers to any modification in the genes other than the change in the DNA sequences itself. It includes how environmental factors acting o ...
Build Your DNA and Eat It Too
Build Your DNA and Eat It Too

... 12. backbone “DNA- 1” or “DNA-2” depending on which sequence you used. Write the label on the left end of the licorice. 13. Label your model. Make flags to label the parts of your DNA out of paper clips and tape. Label one of each of the following: adenine, thymine, cytosine, guanine, and the backbon ...
LE - 7 - Genetic Engineering
LE - 7 - Genetic Engineering

... Gene Encoded on Plasmid ...
MCQ- V-Semester 2015 - KLE College of Pharmacy
MCQ- V-Semester 2015 - KLE College of Pharmacy

... The first drug produced using recombinant DNA technology was: A) Streptokinase ...
DNA PROFILING
DNA PROFILING

... Cells are broken down to release DNA ...
Molecular Biology of the Gene
Molecular Biology of the Gene

... – ATG, GCG, TCA, GGT, CAT… (64 different possible combinations) – each triplet codes for a amino acid of the protein encoded by the gene • a gene that is contains 3,000 nucleotides (1,000 triplets) will code for a protein that consists of 1,000 amino acids ...
- English Longitudinal Study of Ageing
- English Longitudinal Study of Ageing

... Often genes do not actually give rise to a specific illness but may pre-dispose to one. Two people may both be pre-disposed to a particular illness, but only one person actually suffers from it. Why? What triggers the onset of the illness? Is it something to do with the environment? Or is it other g ...
cloning vectors
cloning vectors

... Lysogenic infection cycle: The pattern of phage infection that involves integration of the phage DNA into the host chromosome. Lytic infection cycle: The pattern of infection displayed by a phage that replicates and lyses the host cell immediately after the initial infection. Integration of the phag ...
Molecular Biology
Molecular Biology

... – ATG, GCG, TCA, GGT, CAT… (64 different possible combinations) – each triplet codes for a amino acid of the protein encoded by the gene • a gene that is contains 3,000 nucleotides (1,000 triplets) will code for a protein that consists of 1,000 amino acids ...
DNA TECHNOLOGY AND GENOMICS
DNA TECHNOLOGY AND GENOMICS

... • Thus, pieces of DNA that have been cut with the same restriction enzyme can bind together to form a new sequence of nucleotides. ...
Molecular Biology
Molecular Biology

... Lysogenic infection cycle: The pattern of phage infection that involves integration of the phage DNA into the host chromosome. Lytic infection cycle: The pattern of infection displayed by a phage that replicates and lyses the host cell immediately after the initial infection. Integration of the phag ...
Wiki - DNA Fingerprinting, Individual Identification and Ancestry
Wiki - DNA Fingerprinting, Individual Identification and Ancestry

... Discussion Forums @ AMNH ...
ENZYMES AS TOOLS IN GENE MANIPULATION
ENZYMES AS TOOLS IN GENE MANIPULATION

... The vast majority of type II enzymes recognize and break a double stranded DNA sequence that possesses a twofold axis of rotational symmetry, reading the same 5´ to 3´ direction along the upper strand as it does along the lower strand: ...
Too good to be true? DNA sequencing by Oxford Nanopore. Now.
Too good to be true? DNA sequencing by Oxford Nanopore. Now.

DNA Sequencing as a Method for Larval Identification in Odonates
DNA Sequencing as a Method for Larval Identification in Odonates

... In addition to identification purposes, DNA sequencing is important in phylogenetic research. The 16S rRNA and rDNA regions of the mitochondrial genome have often been used to identify odonate species relationships (Misof et al 2000; Hasegawa & Kasuya 2006; Pilgrim & Dohlen 2008). In more recent st ...
Do = 228 (155-432) [tg/ml
Do = 228 (155-432) [tg/ml

... somal aberrations induced by radiation and by chemicals, even if the cells were exposed to mutagens in the same phase of cell cycle. INTERACTION OF ACTINOMYCIN Non-random distribution of breaks of indiD, RADIATION AND YEAST CELL vidual chromosomes as well as especially SURVIVAL. E. VAN DUYSE, A. DUN ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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