• Study Resource
  • Explore Categories
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
You are going to experiment (on paper) with the somatastatin
You are going to experiment (on paper) with the somatastatin

... If the codon is changed or mutated such that a C-G pair is substituted for the correct A-T pair that is normally found in the third position, the degeneracy of the code will prevent any change in the overall resulting protein. The codon UGC will also code for Cys and the protein will not be changed. ...
Pentose sugars
Pentose sugars

Karina Espinoza - Werner Syndrome
Karina Espinoza - Werner Syndrome

...  Avoidance of smoking, excess weight, & inactivity (increase the risk of atherosclerosis)  Skin care ...
Intro, show Jurassic Park, relate to all other units, Discuss history
Intro, show Jurassic Park, relate to all other units, Discuss history

... The sides of the ladder are made up of sugars and phosphates. The rungs are nitrogen bases. The bases exhibit complementary base pairing. 10 base pairs per turn of the helix. The sugar and phosphates never change but the nitrogen bases are unique for each gene. Gene –sequence of nucleotides that con ...
Isolation and amplification of ancient DNA
Isolation and amplification of ancient DNA

... Ancient DNA (aDNA) analyses rely on the extraction of the minute amount of DNA remaining in a sample which can be hundreds to tens of thousands of years old. Obviously, the efficiency of DNA extraction from archaeological sample plays a key role for the whole analytical process. aDNA studies share a ...
5.3 Presentation: Protein Synthesis
5.3 Presentation: Protein Synthesis

... • Cells respond to their environments by producing different types and amounts of proteins • The cell produces proteins that are structural (forms part of cell materials) or functional (enzymes and hormones). • All of an organisms cells have the same DNA, but the cells differ on the expression of th ...
Chapter 12-1 Part 2
Chapter 12-1 Part 2

... 3. Bacteria Extract + Carb destroying enzymes = transformation occurred (mouse alive or dead?) 4. Bacteria Extract + RNA destroying enzymes = transformation occurred (mouse alive or dead?) 5. Bacteria Extract + DNA destroying enzymes = transformation DID NOT OCCUR (mouse alive or dead?) ...
Document
Document

... 14. What is the haploid number of chromosomes for a human sperm or egg cell? 15. What is the number of chromosomes in a body cell known as? 16. Who is responsible for discovering the structure of DNA (two names)? 17. _______________stands for deoxyribonucleic acid. 18. _______________stands for ribo ...
Genetics Module B, Anchor 2 Basic Mendelian Genetics: 1. Different
Genetics Module B, Anchor 2 Basic Mendelian Genetics: 1. Different

... 5. Which of the following forms a Barr body: C. one of the X chromosomes in a female cell Genetic Engineering: 1. Organisms that contain genes from other organisms are called A. transgenic 2. Describe what happens during a polymerase chain reaction. What is the use of PCR? The first step in using th ...
The Genetic Code and Transcription Chapter 12 Honors Genetics
The Genetic Code and Transcription Chapter 12 Honors Genetics

Lesson 34 - Science with Mr Thompson
Lesson 34 - Science with Mr Thompson

... Why do cells make an exact copy of their DNA in S phase? What is mitosis? What terms have you come across so far that are closely associated with the term DNA? ...
Manipulating DNA
Manipulating DNA

...  2. Look carefully at the series, and find this sequence of letters: GTTAAC. It may appear more than once. How many occurrences of the sequence GTTAAC can you find? ...
Concepts of Genetics Necessities of Life Reproduction: DNA DNA
Concepts of Genetics Necessities of Life Reproduction: DNA DNA

... –22 pairs of autosomal chromosomes affecting almost all aspects of the individual other than sex –1 set of sex determining chromosomes •A pair of X chromosomes for Females •One X and one Y chromosome for Males ...
Chapter 12: Genetic Engineering
Chapter 12: Genetic Engineering

... DNA fragments cannot function all by themselves They must become a part of the genetic material of living cells before the genes they contain can be activated In the second step of genetic engineering, DNA fragments are incorporated into part of the recipient cell’s genetic material ...
The Tools of Molecular Biology How do scientists make changes to
The Tools of Molecular Biology How do scientists make changes to

... The Tools of Molecular Biology How do scientists make changes to DNA? ...
Cells and Enzymes
Cells and Enzymes

... _________________________________________________________________ _________________________________________________(1) b. Name the enzyme responsible for the primary transcript. ________________________________________________________(1) c. Name the process that involves the modification of the prim ...
2013 Gen Tech Part 2
2013 Gen Tech Part 2

... The Tools of Molecular Biology How do scientists make changes to DNA? ...
5 In vivo gene cloning
5 In vivo gene cloning

Genetic conditions - Centre for Genetics Education
Genetic conditions - Centre for Genetics Education

Genetics 200A 2009 Prokaryotes Lecture 1 (Cox)
Genetics 200A 2009 Prokaryotes Lecture 1 (Cox)

... Results: Alan Campbell isolated 130 mutants: they grow in bacterial strain C600 (suII+) but not in wild-type bacterial strain such as 594 (su°). Do the mutations affect different functions/genes? This can be determined by doing pairwise co-infections with individual mutants. It is important that mo ...
PCR and Its Applications
PCR and Its Applications

... What is PCR? : The “Reaction” Components 1) Target DNA - contains the sequence to be amplified. 2) Pair of Primers - oligonucleotides that define the sequence to be amplified. 3) dNTPs - deoxynucleotidetriphosphates: DNA building blocks. 4) Thermostable DNA Polymerase - enzyme that catalyzes the re ...
BIOLOGY 12 MUTATIONS FRAMESHIFT MUTATIONS
BIOLOGY 12 MUTATIONS FRAMESHIFT MUTATIONS

... sequence. This type of mutation can change the gene's protein product in the following ways: • Missense mutations are point mutations that result in a single amino acid change within the protein. UAC – CAC – this leads to a change in shape of protein and its function. The ...
Cellulase gene cloning
Cellulase gene cloning

... amplified from the plasmid pGREGbgl1using primers pRSPGK_F and pRSCYC_R (Table S1), which each contain 35 nts homologous to the multicloning site (MCS) of the pRSH plasmid (3). The amplified DNA fragment was mixed in a molar ratio of 10:1 with pRSH, linearised with KpnI and SacI within the MCS, and ...
Microbial Genetics
Microbial Genetics

... structures, enzymes or regulators. • Most gene products will be a polypeptide, which fold-up into functions proteins. • The instructions are manifest as a unique sequence of nucleotide base pairs within a larger DNA molecule. • A universal genetic code is followed to convert base pair sequence infor ...
ppt
ppt

... The difference is based on two ring forms for glucose: ...
< 1 ... 517 518 519 520 521 522 523 524 525 ... 766 >

Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
  • studyres.com © 2026
  • DMCA
  • Privacy
  • Terms
  • Report