Replication of the DNA
... 2) We have some problems to use this – Our vector would be chopped into pieces, not merely opened coveniently if there were more than one cut site in the vector – We must avoid inserting the cloned gene into any of the genes needed by the plasmid for its own replication and survival within the cell ...
... 2) We have some problems to use this – Our vector would be chopped into pieces, not merely opened coveniently if there were more than one cut site in the vector – We must avoid inserting the cloned gene into any of the genes needed by the plasmid for its own replication and survival within the cell ...
Protein Synthesis Notes
... found in DNA. b. Carries instructions to the ribosomes on how to make a specific protein. ...
... found in DNA. b. Carries instructions to the ribosomes on how to make a specific protein. ...
Lecture 2
... Insertion of DNA into the MCS located within the lacZ gene (codons 6-7 of lacZ are replaced by MCS) inactivates the N-terminal fragment of betagalactosidase and abolishes alfa-complementation. Bacteria carrying recombinant plasmids therefore give rise to white colonies. ...
... Insertion of DNA into the MCS located within the lacZ gene (codons 6-7 of lacZ are replaced by MCS) inactivates the N-terminal fragment of betagalactosidase and abolishes alfa-complementation. Bacteria carrying recombinant plasmids therefore give rise to white colonies. ...
REVIEW for EXAM4-May 12th
... in this process because it is the first step and determines whether the gene will be transcribed in the first place. Note: in prokaryotes or bacteria, both transcription and translation can occur simultaneously because bacterial DNA is not confined in a nuclear membrane. Let’s dissect these mechanis ...
... in this process because it is the first step and determines whether the gene will be transcribed in the first place. Note: in prokaryotes or bacteria, both transcription and translation can occur simultaneously because bacterial DNA is not confined in a nuclear membrane. Let’s dissect these mechanis ...
Chapter 4 - WordPress.com
... • Protein is composed of a series of amino acids linked together by peptide bonds in a specific sequence. • Proteins are used primarily in the synthesis of hormones, enzymes, antibodies, plasma proteins, muscle proteins, hemoglobin, and cell membranes. Proteins are also used as fuel and as raw mater ...
... • Protein is composed of a series of amino acids linked together by peptide bonds in a specific sequence. • Proteins are used primarily in the synthesis of hormones, enzymes, antibodies, plasma proteins, muscle proteins, hemoglobin, and cell membranes. Proteins are also used as fuel and as raw mater ...
11_DNA is the genetic material (MRU)
... While DNA is the genetic material for the vast majority of organisms, there are some viruses that use RNA as their genetic material. These viruses can be either single or double stranded. Examples include SARS, influenza, hepatitis C and polio, as well as the ret ...
... While DNA is the genetic material for the vast majority of organisms, there are some viruses that use RNA as their genetic material. These viruses can be either single or double stranded. Examples include SARS, influenza, hepatitis C and polio, as well as the ret ...
Key
... C. allows crossing over during meiosis. D. removes exons from an RNA molecule. E. occurs in the cytosol. 8. The enhancers located near the albumin gene A. are only present in liver cells. B. bind transcription factors only found in the liver. C. are located in introns. D. change the position at whic ...
... C. allows crossing over during meiosis. D. removes exons from an RNA molecule. E. occurs in the cytosol. 8. The enhancers located near the albumin gene A. are only present in liver cells. B. bind transcription factors only found in the liver. C. are located in introns. D. change the position at whic ...
Reading Guide_08_EB_TandT
... While reading these chapters, constantly ask yourself, “How is this information helping me to understand how my cells respond to the environment and reproduce?” Chapter 1, pg 7-8 (Cells and Their DNA) 1. How do prokaryotes and eukaryotes store their DNA? 2. What are genes? 3. Do bacteria and humans ...
... While reading these chapters, constantly ask yourself, “How is this information helping me to understand how my cells respond to the environment and reproduce?” Chapter 1, pg 7-8 (Cells and Their DNA) 1. How do prokaryotes and eukaryotes store their DNA? 2. What are genes? 3. Do bacteria and humans ...
See DNA Essay possibilities
... A bacterial plasmid is 100 kb in length. The plasmid DNA was digested to completion with two restriction enzymes in three separate treatments: EcoRI, HaeIII, and EcoRI + HaeIII (double digest). The fragments were then separated with electrophoresis, as shown. a) Using the circle provided, CONSTRUCT ...
... A bacterial plasmid is 100 kb in length. The plasmid DNA was digested to completion with two restriction enzymes in three separate treatments: EcoRI, HaeIII, and EcoRI + HaeIII (double digest). The fragments were then separated with electrophoresis, as shown. a) Using the circle provided, CONSTRUCT ...
Biology Chp 13 Gene Technology
... a. DNA found at crime scenes often small amounts b. must be copied to have enough for identification c. Polymerase Chain Reaction (PCR) quickly produces many copies of a DNA fragment. 1. Primers: artificially made single DNA strands 20 to 30 nucleotides long 2. Know 4 steps on page 256 2. CUTTING DN ...
... a. DNA found at crime scenes often small amounts b. must be copied to have enough for identification c. Polymerase Chain Reaction (PCR) quickly produces many copies of a DNA fragment. 1. Primers: artificially made single DNA strands 20 to 30 nucleotides long 2. Know 4 steps on page 256 2. CUTTING DN ...
DNA Technology and Genomes
... Gene Cloning: making multiple identical copies of a gene (specific pieces of DNA) Clone: a group of genetically identical organisms or a group of cells derived from a single parent cell. Plasmid: circular DNA found in bacteria, not part of the nucleoid region Restriction Enzymes: enzymes that protec ...
... Gene Cloning: making multiple identical copies of a gene (specific pieces of DNA) Clone: a group of genetically identical organisms or a group of cells derived from a single parent cell. Plasmid: circular DNA found in bacteria, not part of the nucleoid region Restriction Enzymes: enzymes that protec ...
Proteins
... human antibodies. How is this possible with only ~30,000 genes? • Alternative splicing refers to the different ways of combining a gene’s exons. This can produce different forms of a protein for the ...
... human antibodies. How is this possible with only ~30,000 genes? • Alternative splicing refers to the different ways of combining a gene’s exons. This can produce different forms of a protein for the ...
DNA Mutation
... Recombination This is a breakage and reunion process. Homologous parts of chromosome come into apposition, at which point both strands are broken and then reconnected in a crosswise fashion ...
... Recombination This is a breakage and reunion process. Homologous parts of chromosome come into apposition, at which point both strands are broken and then reconnected in a crosswise fashion ...
Cell Division
... divides into four nuclei each containing half the chromosome number leading to gametes. ...
... divides into four nuclei each containing half the chromosome number leading to gametes. ...
DNA Technology
... Using the technology of recombinant DNA, we are able to introduce specific genes from one organism into another. A transgenic organism is an organism that has been genetically engineered to contain 1 or more genes ...
... Using the technology of recombinant DNA, we are able to introduce specific genes from one organism into another. A transgenic organism is an organism that has been genetically engineered to contain 1 or more genes ...
CELL CHEMISTRY QUESTIONS 1. - Queensland Science Teachers
... 22. Different types of cells have different proteins. How can this be used to identify cell types? 23. Is every protein composed of all possible amino acids? Explain. 24. Why are some amino acids called essential amino acids? 25. What are some of the many functions of proteins? 26. Nucleic acids are ...
... 22. Different types of cells have different proteins. How can this be used to identify cell types? 23. Is every protein composed of all possible amino acids? Explain. 24. Why are some amino acids called essential amino acids? 25. What are some of the many functions of proteins? 26. Nucleic acids are ...
1 Basic Genomics 1. How do you sequence DNA? Two methods
... Repetitive DNA – long stretches of the same DNA sequence repeated many times in tandem. This makes up most of the heterochromatin. It is enriched at centromeres and telomeres. Transposable elements (TE’s) – pieces of DNA that can replicate and move within the genome. Also known as “Interspersed repe ...
... Repetitive DNA – long stretches of the same DNA sequence repeated many times in tandem. This makes up most of the heterochromatin. It is enriched at centromeres and telomeres. Transposable elements (TE’s) – pieces of DNA that can replicate and move within the genome. Also known as “Interspersed repe ...
PowerPoint 簡報
... RNA splicing or introduce premature stop codons, which inactivate them. These dead genes are called pseudogenes. • These processed pseudogenes have probably been produced by the reverse transcription of the mature mRNA transcript of a gene (which will itself lack introns and promoter sequences. • Ps ...
... RNA splicing or introduce premature stop codons, which inactivate them. These dead genes are called pseudogenes. • These processed pseudogenes have probably been produced by the reverse transcription of the mature mRNA transcript of a gene (which will itself lack introns and promoter sequences. • Ps ...
Biol 213 Genetics (13 September 2000) Relationship between
... SQ21. What if Beadle and Tatum analyzed the original irradiated haploid spores and did not analyze spores from the heterozygous strain. What information would they have missed? III. RNA and an overview of gene expression (pp.238-240; pp.321-325) We’ve established a connection between DNA and protein ...
... SQ21. What if Beadle and Tatum analyzed the original irradiated haploid spores and did not analyze spores from the heterozygous strain. What information would they have missed? III. RNA and an overview of gene expression (pp.238-240; pp.321-325) We’ve established a connection between DNA and protein ...
Sc9 - a 4.2 (teacher notes)
... What are the risks of cloning? Reproductive cloning is expensive and highly inefficient. More than 90% of cloning attempts fail to produce viable offspring. More than 100 nuclear transfer procedures could be required to produce one viable clone. In addition to low success rates, cloned animals tend ...
... What are the risks of cloning? Reproductive cloning is expensive and highly inefficient. More than 90% of cloning attempts fail to produce viable offspring. More than 100 nuclear transfer procedures could be required to produce one viable clone. In addition to low success rates, cloned animals tend ...
When Is A Worm Not A Worm? When It`s A Jellyfish
... Its bizarre body-plan is quite unlike other worms, such as nematodes, that despite their simplicity have different ‘organs’ on different sides – and at different ends – of their bodies in the same way that fish and mammals do. Professor Holland said: ‘Buddenbrockia is very unusual in not displaying ...
... Its bizarre body-plan is quite unlike other worms, such as nematodes, that despite their simplicity have different ‘organs’ on different sides – and at different ends – of their bodies in the same way that fish and mammals do. Professor Holland said: ‘Buddenbrockia is very unusual in not displaying ...
Cre-Lox recombination
In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.