Lecture 6
... Two methods have been used recently: 1. Conventional genome sequencing-low resolution maps made by identifying “landmarks” in ~250 kb inserts in YACs. Landmarks are 200-300 bp segments, aka sequence tagged sites(STSs)-2 clones with the same STS overlap. STS-containing inserts are sheared randomly in ...
... Two methods have been used recently: 1. Conventional genome sequencing-low resolution maps made by identifying “landmarks” in ~250 kb inserts in YACs. Landmarks are 200-300 bp segments, aka sequence tagged sites(STSs)-2 clones with the same STS overlap. STS-containing inserts are sheared randomly in ...
Unit 3 Biochemistry
... The stages of mitosis are shown out of order below. Label them with the correct name and put a number next to each to show the correct order, beginning with prophase. ...
... The stages of mitosis are shown out of order below. Label them with the correct name and put a number next to each to show the correct order, beginning with prophase. ...
Biochemistry
... • They make up the structural parts of cells, enzymes, antibodies, hormones and membrane proteins. • Chemically they consist of an amine group (NH2) and a carboxyl group (COOH) and an “R” group. • There are 20 different R groups ...
... • They make up the structural parts of cells, enzymes, antibodies, hormones and membrane proteins. • Chemically they consist of an amine group (NH2) and a carboxyl group (COOH) and an “R” group. • There are 20 different R groups ...
BIOT 3 Lab 3 Handout 1
... defense mechanism to protect against infectious pathogens such as viruses called bacteriophage, also known as phage. Phage viruses reproduce by injecting DNA into a host bacteria and then use the host’s cellular machinery to replicate more copies of phage virus. Bacteria produce restriction enzymes ...
... defense mechanism to protect against infectious pathogens such as viruses called bacteriophage, also known as phage. Phage viruses reproduce by injecting DNA into a host bacteria and then use the host’s cellular machinery to replicate more copies of phage virus. Bacteria produce restriction enzymes ...
Lab Manual: Week 8
... thymine dimers are formed, the DNA is distorted and the thymines fail to pair with the adenines on the opposite strands, thus causing a mutation. The bactericidal effects of ultraviolet rays are limited to a specific region of the ultraviolet spectrum. The radiation found to be both the most lethal ...
... thymine dimers are formed, the DNA is distorted and the thymines fail to pair with the adenines on the opposite strands, thus causing a mutation. The bactericidal effects of ultraviolet rays are limited to a specific region of the ultraviolet spectrum. The radiation found to be both the most lethal ...
formativeassessment - the Biology Scholars Program Wiki
... As a group, write down what you know about DNA and proteins on one side of the white board. On the other side, write what else you need to know to be able to answer this question. ...
... As a group, write down what you know about DNA and proteins on one side of the white board. On the other side, write what else you need to know to be able to answer this question. ...
DNA
... The smallest DNA in nature is virus DNA. The length of φX174 virus DNA is 5,386 bases (a single chain). ...
... The smallest DNA in nature is virus DNA. The length of φX174 virus DNA is 5,386 bases (a single chain). ...
E. coli - JonesHonorsBioBlue
... DNA has been chemically modified by other enzymes in a way that protects it from the restriction enzymes. Most restriction enzymes recognize short nucleotide sequences in DNA molecules and cut at specific points within these recognition sequences. Several hundred restriction enzymes and about a hund ...
... DNA has been chemically modified by other enzymes in a way that protects it from the restriction enzymes. Most restriction enzymes recognize short nucleotide sequences in DNA molecules and cut at specific points within these recognition sequences. Several hundred restriction enzymes and about a hund ...
Protein Synthesis Project
... ____________________________________________________________________________________________________________ ____________________________________________________________________________________________________________ c. Which do you think would cause a more profound biological impact: (1) a deletio ...
... ____________________________________________________________________________________________________________ ____________________________________________________________________________________________________________ c. Which do you think would cause a more profound biological impact: (1) a deletio ...
Esperimento di genetica 17.1
... EG17.1.2. As seen here, the chromosomes show the classic harlequin appearance due to the differential staining of the sister chromatids. Furthermore, examples of SCE are clearly visible. The arrows depict regions where crossing over has taken place. In this study, Perry and Wolff found that SCEs occ ...
... EG17.1.2. As seen here, the chromosomes show the classic harlequin appearance due to the differential staining of the sister chromatids. Furthermore, examples of SCE are clearly visible. The arrows depict regions where crossing over has taken place. In this study, Perry and Wolff found that SCEs occ ...
Exam3-1406_Fall2007ch9-10-11.doc
... C) addition of exons to the mRNA. D) deletion of exons from the mRNA. E) combination of two different genes together. ...
... C) addition of exons to the mRNA. D) deletion of exons from the mRNA. E) combination of two different genes together. ...
Acc_Bio_DNA_Webquest
... 18. Give an example of how an environmental factor can influence a trait. ...
... 18. Give an example of how an environmental factor can influence a trait. ...
DNA Tech WebQuest
... Only a small sample of DNA is needed for DNA fingerprinting - a hair or a semen sample is plenty. A special process called PCR (polymerase chain reaction) is used to copy this DNA millions of times so that the scientists have plenty of copies to work with. It is vital that the sample of DNA is not c ...
... Only a small sample of DNA is needed for DNA fingerprinting - a hair or a semen sample is plenty. A special process called PCR (polymerase chain reaction) is used to copy this DNA millions of times so that the scientists have plenty of copies to work with. It is vital that the sample of DNA is not c ...
Biochemistry
... Proteins are the most complex macromolecules in the cell. They are composed of linear polymers called polypeptides, which contain amino acids connected by peptide bonds. ...
... Proteins are the most complex macromolecules in the cell. They are composed of linear polymers called polypeptides, which contain amino acids connected by peptide bonds. ...
General Biology I (BIOLS 102)
... Elongation factors – proteins that facilitate complementary base pairing between the tRNA anticodon and the mRNA codon at the ribosome The polypeptide is transferred and attached by a peptide bond to the newly arrived amino acid in the A site via a ribozyme and energy (ATP) ...
... Elongation factors – proteins that facilitate complementary base pairing between the tRNA anticodon and the mRNA codon at the ribosome The polypeptide is transferred and attached by a peptide bond to the newly arrived amino acid in the A site via a ribozyme and energy (ATP) ...
Eukaryotic Genomes Chapter 19
... gene duplications that occur as errors during DNA replication and recombination. ► The differences in genes arise from mutations that accumulate in the gene copies over generations. These mutations may even lead to enough changes to form pseudogenes, DNA segments that have sequences similar to rea ...
... gene duplications that occur as errors during DNA replication and recombination. ► The differences in genes arise from mutations that accumulate in the gene copies over generations. These mutations may even lead to enough changes to form pseudogenes, DNA segments that have sequences similar to rea ...
femurhvj
... ancestors, and all things are conceived of as possessing a life force or mauri, since all living things are connected by a common descent through whakapapa or genealogy. Certain people and objects contain manaspiritual power or essence. In accordance with the Polynesian tradition, Tangaroa is god of ...
... ancestors, and all things are conceived of as possessing a life force or mauri, since all living things are connected by a common descent through whakapapa or genealogy. Certain people and objects contain manaspiritual power or essence. In accordance with the Polynesian tradition, Tangaroa is god of ...
Notes to Educators
... Notes to Educators Amino Acid Properties Amino acids share a common backbone, through which the amino acids are joined by peptide bonds to build proteins. The amino acid sidechains, however, vary in structure and chemical properties. The properties of these sidechains (non-polar, polar but uncharged ...
... Notes to Educators Amino Acid Properties Amino acids share a common backbone, through which the amino acids are joined by peptide bonds to build proteins. The amino acid sidechains, however, vary in structure and chemical properties. The properties of these sidechains (non-polar, polar but uncharged ...
Modeling Genetic Engineering Lab
... The enzyme cards illustrate a short DNA sequence that each enzyme can cut. Compare the base sequence on each enzyme card with the base sequence of the plasmid. Some restriction enzymes may be able to cut open the plasmid in multiple locations while others may not be able to cut open the plasmid at a ...
... The enzyme cards illustrate a short DNA sequence that each enzyme can cut. Compare the base sequence on each enzyme card with the base sequence of the plasmid. Some restriction enzymes may be able to cut open the plasmid in multiple locations while others may not be able to cut open the plasmid at a ...
Biology 303 EXAM II 3/14/00 NAME
... cis-acting, variable orientation, variable position trans-acting, fixed position, fixed orientation cis-acting, fixed position, fixed orientation trans-acting, variable position, fixed orientation ...
... cis-acting, variable orientation, variable position trans-acting, fixed position, fixed orientation cis-acting, fixed position, fixed orientation trans-acting, variable position, fixed orientation ...
Inquiry into Life Twelfth Edition
... Using Reverse Transcriptase PCR (RTPCR) in cDNA Cloning • To clone a cDNA from just one mRNA whose sequence is known, a type of PCR called reverse transcriptase PCR (RT-PCR) can be used • Difference between PCR and RT-PCR – Starts with an mRNA, not dsDNA – Begin by converting mRNA to DNA – Use forw ...
... Using Reverse Transcriptase PCR (RTPCR) in cDNA Cloning • To clone a cDNA from just one mRNA whose sequence is known, a type of PCR called reverse transcriptase PCR (RT-PCR) can be used • Difference between PCR and RT-PCR – Starts with an mRNA, not dsDNA – Begin by converting mRNA to DNA – Use forw ...
DNA
... Before the mRNA can go to the ribosome, it needs to be spliced. – The junk (parts of the DNA that are noncoding regions) called introns need to be cut out. – Exons (coding regions) are then stuck together. This is the correct concise message. ...
... Before the mRNA can go to the ribosome, it needs to be spliced. – The junk (parts of the DNA that are noncoding regions) called introns need to be cut out. – Exons (coding regions) are then stuck together. This is the correct concise message. ...
DNA your onions? - ncbe.reading.ac.uk
... household detergent and salty water. Onions are the best material to use because their cells contain a relatively large amount of DNA (1C = 415 Mb). They are cheap and available throughout the year, and unlike some plant materials, are highly unlikely to cause allergic reactions. ...
... household detergent and salty water. Onions are the best material to use because their cells contain a relatively large amount of DNA (1C = 415 Mb). They are cheap and available throughout the year, and unlike some plant materials, are highly unlikely to cause allergic reactions. ...
Structure and Function in Biochemistry
... chromosomes, before cell division; each daughter cell contains one copy of the chromosome of the parent and several copies of the plasmid. The information in the structural genes of the plasmid is translated into protein so that the cell synthesizes proteins encoded both by its chromosomal and plasm ...
... chromosomes, before cell division; each daughter cell contains one copy of the chromosome of the parent and several copies of the plasmid. The information in the structural genes of the plasmid is translated into protein so that the cell synthesizes proteins encoded both by its chromosomal and plasm ...
Cre-Lox recombination
In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.