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DNA
DNA

... Before the mRNA can go to the ribosome, it needs to be spliced. – The junk (parts of the DNA that are noncoding regions) called introns need to be cut out. – Exons (coding regions) are then stuck together. This is the correct concise message. ...
Maintaining integrity
Maintaining integrity

... member of the bZIP family of transcription factors. Interestingly, this transcription factor, in response to DSBs, functions as a typical damage response protein: ATM dependent phosphorylation results in migration to damage-associated nuclear foci. Loss of ATF2 attenuated the recruitment of Mre11 to ...
Structure and Function in Biochemistry
Structure and Function in Biochemistry

... chromosomes, before cell division; each daughter cell contains one copy of the chromosome of the parent and several copies of the plasmid. The information in the structural genes of the plasmid is translated into protein so that the cell synthesizes proteins encoded both by its chromosomal and plasm ...
DNA: The Hereditary Molecule
DNA: The Hereditary Molecule

... certainly no molecule is mentioned in the news more than DNA is. DNA evidence makes the news with great regularity. It has been introduced as evidence in highly publicized murder trials and is now regularly being used to reexamine the guilt or innocence of prisoners convicted of violent crimes many ...
Teacher quality grant
Teacher quality grant

... DNA. In eukaryotes, this information can be found in the nucleus of virtually every cell. Eukaryotic cell Nucleus CHROMOSOME One or more unique pieces of DNA—circular in prokaryotes, linear in eukaryotes—that together make up an organism's genome. Chromosomes vary in length and can consist of hundre ...
DNA - Gulf Coast State College
DNA - Gulf Coast State College

... DNA. In eukaryotes, this information can be found in the nucleus of virtually every cell. Eukaryotic cell Nucleus CHROMOSOME One or more unique pieces of DNA—circular in prokaryotes, linear in eukaryotes—that together make up an organism's genome. Chromosomes vary in length and can consist of hundre ...
Study Guide
Study Guide

... A double stranded DNA molecule is broken by helicase, which separates the two strands by  breaking the weak hydrogen bonds that links the nitrogenous bases together. Next, DNA  polymerase adds complementary nucleotides to each strand of DNA. The end product is two  identical copies of double strande ...
9.4 Genetic Engineering
9.4 Genetic Engineering

... • Bacterial plasmids are often used to make recombinant DNA. – plasmids are loops of DNA in bacteria – restriction enzymes cut plasmid and foreign DNA – foreign gene inserted into plasmid – Vector – bacteriophage, plasmid or other agent that transfers genetic material from one cell to another. ...
Chapter 17 Guided Notes
Chapter 17 Guided Notes

... The interpreter is transfer RNA (tRNA), which transfers ____________________________________ ___________________________________________________________________________________. ○ A cell has all _____ amino acids available in its cytoplasm, either by synthesizing them from scratch or by ____________ ...
Homologous chromosomes
Homologous chromosomes

... – Many people have the same number of repeats in a certain region of DNA – The probability that two people share identical numbers of repeats in several locations is very small – Several regions of DNA are used to make a DNA fingerprint to make it more likely the fingerprint is unique. ...
Genetic Mapping
Genetic Mapping

PowerPoint
PowerPoint

... • Occurs during lytic cycle of virulent phage • During viral assembly, fragments of host DNA mistakenly packaged into phage head – generalized transducing particle ...
Mutations
Mutations

... • Occurs during lytic cycle of virulent phage • During viral assembly, fragments of host DNA mistakenly packaged into phage head – generalized transducing particle ...
Molecular Typing Of microorganisms
Molecular Typing Of microorganisms

... instead of constantly running the voltage in one direction, the voltage is periodically switched among three directions; • Time consuming • Expensivethe central axis of the gel  one that runs through LIMITATIONS • Specialized equipment and two that run at an angle of 60 degrees either side ...
doc BIOL200 quiz 4 afternoon
doc BIOL200 quiz 4 afternoon

... Plasmids are naturally occurring in bacteria, and can transfer genetic information to other bacteria. The reason why plasmids engineered by molecular biologists are abundant in transformed bacteria and can yield large amounts of DNA is that they are equally segregated during bacterial division. Fore ...
PH_Genetics__Natural..
PH_Genetics__Natural..

...  The mRNA carries the code from the nucleus to the ribosomes in the cytoplasm.  RNA is a single-stranded polymer of four nucleotide monomers. A RNA nucleotide is identified by the base it contains-adenine, guinine, cyctosine or uracil.  At the ribosome, amino acids are linked to form specific pro ...
Chapter 2. Nucleic Acids
Chapter 2. Nucleic Acids

... physiological and pathological processes in the body at molecular levels, and more importantly, to use the knowledge to . 2. Biochemistry is also a powerful tool in life-scientific studies—prepares you to be a good scientist. ...
Biology CST Practice Questions
Biology CST Practice Questions

... 35. Although there are a limited number of amino acids, many different types of proteins exist because the A. size of a given amino acid can vary. B. chemical composition of a given amino acid can vary. C. sequence and number of amino acids is different. D. same amino acid can have many different p ...
DNA topology and genome organization in higher eukaryotes
DNA topology and genome organization in higher eukaryotes

syllabus components
syllabus components

... understand the relationship between random segregation, independent assortment, and meiosis; test predictions of Mendelian crosses using the Chi-square Test; list sex determination mechanisms in organisms such as mammals, birds, and insects; describe how sex chromosome linked genes affect expected M ...
Biology 102 Lecture 12: From DNA to Proteins
Biology 102 Lecture 12: From DNA to Proteins

... Introns must be removed and exons joined together Called RNA splicing ...
THE NUCLEIC ACIDS
THE NUCLEIC ACIDS

... - the conversion by tRNA to protein at the ribosome (translation) • Transcription takes place in the nucleus, while translation takes place in the cytoplasm • Genetic information is transcribed to form mRNA much the same way it is replicated during cell division ...
DOC - San Juan College
DOC - San Juan College

... understand the relationship between random segregation, independent assortment, and meiosis; test predictions of Mendelian crosses using the Chi-square Test; list sex determination mechanisms in organisms such as mammals, birds, and insects; describe how sex chromosome linked genes affect expected M ...
third of four for Chapter 9
third of four for Chapter 9

... Phage structure ...
Mutations
Mutations

... Chromosome mutations can result in changes in the number of chromosomes in a cell or changes in the structure of a chromosome. ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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