Cell Division Studies of Escherichia Coli: Expression and Protein
... Progress has been made in understanding cell division at the genetic level. Conditional lethal mutants that cannot form septa have been isolated and referred to as filamentation temperature-sensitive (fts) mutants. septum are ...
... Progress has been made in understanding cell division at the genetic level. Conditional lethal mutants that cannot form septa have been isolated and referred to as filamentation temperature-sensitive (fts) mutants. septum are ...
Supplementary Figure Legends (doc 52K)
... Supplementary Figure S2. Distribution of the ‘heavy’ and ‘light’ DNA in separated SIP fractions. The top of each panel shows the DGGE profiles of bacterial PCR products from separated (a) [13C]-phenanthrene fractions aligned to equivalent [12C]-phenanthrene fractions, (b) [13C]naphthalene fractions ...
... Supplementary Figure S2. Distribution of the ‘heavy’ and ‘light’ DNA in separated SIP fractions. The top of each panel shows the DGGE profiles of bacterial PCR products from separated (a) [13C]-phenanthrene fractions aligned to equivalent [12C]-phenanthrene fractions, (b) [13C]naphthalene fractions ...
Strategies for Attaching Oligonucleotides to Solid Supports
... 3.3.1 Liquid Arrays The availability of large sets of fluorescent micro-spheres that can be used together allows for highly multiplexed assays and efficient sample processing. For example, using a set of 100 distinct fluorescent beads in a multiplex format could generate almost 10,000 unique data po ...
... 3.3.1 Liquid Arrays The availability of large sets of fluorescent micro-spheres that can be used together allows for highly multiplexed assays and efficient sample processing. For example, using a set of 100 distinct fluorescent beads in a multiplex format could generate almost 10,000 unique data po ...
슬라이드 1
... With the help of specially programmed supercomputers researchers can test new enzyme structures in a virtual environment. Even slight changes in an enzyme can result in amazing improvements in stability • An enzyme consists of several hundreds of amino acids located in a delicate threedimensional st ...
... With the help of specially programmed supercomputers researchers can test new enzyme structures in a virtual environment. Even slight changes in an enzyme can result in amazing improvements in stability • An enzyme consists of several hundreds of amino acids located in a delicate threedimensional st ...
fulltext
... Tardigrades are known for being resistant to extreme conditions, including tolerance to ionising and UV radiation in both the hydrated and the dehydrated state. It is known that these factors may cause damage to DNA. It has recently been shown that single and double DNA strand breaks occur when tard ...
... Tardigrades are known for being resistant to extreme conditions, including tolerance to ionising and UV radiation in both the hydrated and the dehydrated state. It is known that these factors may cause damage to DNA. It has recently been shown that single and double DNA strand breaks occur when tard ...
209 Original Scientific Article THE INFLUENCE OF
... One of the main reason for the incorrect development of embryos derived from somatic cell nuclear transfer is caused by insufficient demethylation of injected somatic chromatin to a state comparable with an early embryonic nucleus. It is already known that the epigenetic enzymes transcription in ooc ...
... One of the main reason for the incorrect development of embryos derived from somatic cell nuclear transfer is caused by insufficient demethylation of injected somatic chromatin to a state comparable with an early embryonic nucleus. It is already known that the epigenetic enzymes transcription in ooc ...
Sleeping Beauty - Weber State University
... identical or equivalent to an ancient element that dispersed in fish genomes in part by horizontal transmission between species. A consensus sequence of a transposase gene of the salmonid subfamily of elements was engineered by eliminating the inactivating mutations. Sleeping Beauty transposase bind ...
... identical or equivalent to an ancient element that dispersed in fish genomes in part by horizontal transmission between species. A consensus sequence of a transposase gene of the salmonid subfamily of elements was engineered by eliminating the inactivating mutations. Sleeping Beauty transposase bind ...
Defining the Epigenetic Mechanism of Asymmetric Cell Division of
... 2007). The three cassettes reside in direct orientation and encompass a 30-kb region in the middle of chromosome 2, which also contains genes unrelated to the mating process. Thus far the S. pombe mating is the only system where the double helical structure of DNA has been demonstrated to confer asy ...
... 2007). The three cassettes reside in direct orientation and encompass a 30-kb region in the middle of chromosome 2, which also contains genes unrelated to the mating process. Thus far the S. pombe mating is the only system where the double helical structure of DNA has been demonstrated to confer asy ...
Coffee, B, Zhang, F, Warren, ST and Reines, D: Acetylated histones are associated with the FMR1 gene in normal but not fragile X syndrome cells. Nature Genetics 22:98-101 (1999).
... Cell culture and drug treatments. EBV-transformed lymphoblastoid cell lines were derived from normal males or males with the typical clinical phenotype of fragile X syndrome. In normal cells, the FMR1 repeat is of normal length and methylation status, whereas those cells derived from patients exhibi ...
... Cell culture and drug treatments. EBV-transformed lymphoblastoid cell lines were derived from normal males or males with the typical clinical phenotype of fragile X syndrome. In normal cells, the FMR1 repeat is of normal length and methylation status, whereas those cells derived from patients exhibi ...
Drosophila - The Solomon H Snyder Department of Neuroscience
... segments might be rearranged by a particular insertion such as an FRT site; 4) which gene might be overexpressed by an inserted regulatory element; or 5) the location of a transgene for recombination with other genetic elements, such as a mutation or other transgenes. Since transposon integrated tra ...
... segments might be rearranged by a particular insertion such as an FRT site; 4) which gene might be overexpressed by an inserted regulatory element; or 5) the location of a transgene for recombination with other genetic elements, such as a mutation or other transgenes. Since transposon integrated tra ...
Transformation Lab - Towson University
... environment. It has a single circular chromosome that contains about five million DNA base pairs, only 1/600th the haploid amount of DNA in a human cell. E.coli may also contain small circular DNA molecules called plasmids (1,000-200,000 DNA base pairs). These plasmids carry genetic information and ...
... environment. It has a single circular chromosome that contains about five million DNA base pairs, only 1/600th the haploid amount of DNA in a human cell. E.coli may also contain small circular DNA molecules called plasmids (1,000-200,000 DNA base pairs). These plasmids carry genetic information and ...
the Role of DNA Sequence Data - International Journal of
... Second, because the adaptive mutant cannot outcompete cells from other ecological populations, each periodic selection event has very little effect on the divergence between populations (14,15,18). Because it purges the diversity within but not between populations, periodic selection increases the d ...
... Second, because the adaptive mutant cannot outcompete cells from other ecological populations, each periodic selection event has very little effect on the divergence between populations (14,15,18). Because it purges the diversity within but not between populations, periodic selection increases the d ...
HLRCC Science
... attach to those three nucleotides and that tRNA will have the amino acid tyrosine attached to it. Tyrosine will then be added to the growing chain of amino acids. There is a third kind of RNA called ribosomal RNA (rRNA) which, with the addition of some proteins, forms a ribosome. A ribosome attaches ...
... attach to those three nucleotides and that tRNA will have the amino acid tyrosine attached to it. Tyrosine will then be added to the growing chain of amino acids. There is a third kind of RNA called ribosomal RNA (rRNA) which, with the addition of some proteins, forms a ribosome. A ribosome attaches ...
Omni-Tube PCR, PCR Reaction,Digest, Ligation, and the 3
... he Omni-Tube PCR system is designed and optimized for performing PCR, digestion, ligation, and purification all in a single tube. No extra tubes, spin-columns, filter plates, silica membrane, magnetic beads, vacuum or filtration steps are needed for purification, and no transfer of reaction mixtures ...
... he Omni-Tube PCR system is designed and optimized for performing PCR, digestion, ligation, and purification all in a single tube. No extra tubes, spin-columns, filter plates, silica membrane, magnetic beads, vacuum or filtration steps are needed for purification, and no transfer of reaction mixtures ...
Horizontal, agarose systems
... real-time monitoring of DNA or RNA migration with the E-Gel® Safe Imager Real-Time Transilluminator. Easy access to the gel The gel cassette is designed to open with Invitrogen’s Gel Knife, so that the gel inside can be accessed readily for excision of specific bands or transferred to a membrane for ...
... real-time monitoring of DNA or RNA migration with the E-Gel® Safe Imager Real-Time Transilluminator. Easy access to the gel The gel cassette is designed to open with Invitrogen’s Gel Knife, so that the gel inside can be accessed readily for excision of specific bands or transferred to a membrane for ...
Aberrant replication timing induces defective chromosome
... Conclusions: The results not only reveal novel functions for ORC2 in chromosome architecture in metazoans, they also suggest that the correct timing of DNA replication may be essential for the assembly of chromatin that is fully competent to undergo mitotic condensation. ...
... Conclusions: The results not only reveal novel functions for ORC2 in chromosome architecture in metazoans, they also suggest that the correct timing of DNA replication may be essential for the assembly of chromatin that is fully competent to undergo mitotic condensation. ...
Here - Personal Genome Project Study Guide
... A. The structure that houses the chromosomes B. The structure that produces energy for cells C. A type of DNA D. Proteins Question 5: Which statement about genes and human disease is most accurate? A. Knowing the DNA sequence of all your genes is sufficient information to predict the occurrence of a ...
... A. The structure that houses the chromosomes B. The structure that produces energy for cells C. A type of DNA D. Proteins Question 5: Which statement about genes and human disease is most accurate? A. Knowing the DNA sequence of all your genes is sufficient information to predict the occurrence of a ...
Rapid detection of multidrug-resistant tuberculosis
... eye, and stored using any simple word-processing programme. Strain-typing itself does not provide direct information about drug susceptibility, but a register of spoligotypes from known MDR isolates may be particularly useful in providing early warning of possible infection with these strains. Whils ...
... eye, and stored using any simple word-processing programme. Strain-typing itself does not provide direct information about drug susceptibility, but a register of spoligotypes from known MDR isolates may be particularly useful in providing early warning of possible infection with these strains. Whils ...
functional analysis of chromatin assembly genes in tetrahymena
... The basic structural unit of chromatin is the nucleosome composed of ~147 base pairs of DNA wrapped around an octamer of histone proteins. Post-translational modifications such as histone acetylation or the substitution of histone variants in place of core histones have been implicated in various ch ...
... The basic structural unit of chromatin is the nucleosome composed of ~147 base pairs of DNA wrapped around an octamer of histone proteins. Post-translational modifications such as histone acetylation or the substitution of histone variants in place of core histones have been implicated in various ch ...
Vitis 37 (3), 119
... The group of "Muscat grapes" ("Moschoudia" in Greece) includes some very interesting cultivars grown all over Europe. The most important are Muscat de Frontignan, Moschato aspro (production of dessert wines), Muscat of Alexandria (a multipurpose variety) and Muscat Hamburg (a table grape variety). T ...
... The group of "Muscat grapes" ("Moschoudia" in Greece) includes some very interesting cultivars grown all over Europe. The most important are Muscat de Frontignan, Moschato aspro (production of dessert wines), Muscat of Alexandria (a multipurpose variety) and Muscat Hamburg (a table grape variety). T ...
PDF
... genome duplication (autopolyploids) or via interspecific hybridization (allopolyploids). The polyploidy-associated effects have been extensively reviewed in regard to expression patterns, environmental stress and chromosomal behavior, to explain changes between the established polyploids and diploid ...
... genome duplication (autopolyploids) or via interspecific hybridization (allopolyploids). The polyploidy-associated effects have been extensively reviewed in regard to expression patterns, environmental stress and chromosomal behavior, to explain changes between the established polyploids and diploid ...
Cre-Lox recombination
In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.