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Identification of markers tightly linked to tomato yellow
Identification of markers tightly linked to tomato yellow

... The tomato (Solanum lycopersicon) is an economically important species of the Solanaceae family, and it is cultivated all over the world for human consumption. Recently, tomato crops have often been infected by tomato yellow leaf curl virus (TYLCV), which causes significant yield losses in tomato (S ...
Genomic scars as biomarkers of homologous recombination
Genomic scars as biomarkers of homologous recombination

... Kbp in length (top box), structural copy number aberrations (CNAs) (bottom left box), and structural rearrangements (bottom right box). The initial state in the germline is shown followed by the corresponding change in the tumor. Mutations that affect regions of less than 1 Kbp are of three basic ty ...
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...  Evolutionary history can tell us a lot about properties of a given gene  Homology can be inferred from similarity between the genes  Searching for Proteins with same or similar functions ...
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DNA Methylation
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... Coprinus cinereus is defective in meiotic DNA repair, chromatid cohesin and homolog pairing [1]; mis4 adherin mutants of the fission yeast Schizosaccharomyces pombe display DNA repair and mitotic sister chromatid cohesion defects [2]; and scc2 adherin mutations in the budding yeast Saccharomyces cer ...
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genes. Numbers of 6-10 copies per genome have

... The SSU genes and flanking regions in TSSU3-2 and TSSU3-8 were sequenced. Using sequences of other SSU genes, the leader peptide, mature coding regions and introns were located. In Figure 2, the sequence of these two genes is shown and compared to that of a previously sequenced tobacco SSU gene, NtS ...
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Homologous recombination in budding yeast expressing the human

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... human Ll promoter, Ll site A, and several cis elements of the type found in eukaryotic genes. Within a region of about 500 bp, there are nine “E boxes,” cis elements that are recognized by the basic helix-loop-helix (bHLH) family of proteins. This observation raises the possibility that cellular tra ...
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Bioinformatics with basic local alignment search tool (BLAST) and

... directional polymerisation of single nucleotide units. Each nucleotide unit of a DNA molecule comprises a deoxyribose sugar, a nitrogenous base (adenine, guanine, cytosine, thymine) and a phosphate group. The nucleotides units are linked by phosphoester bonds which occur between 5' and 3' carbon ato ...
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... It is relatively straightforward to develop an empirical system for estimating gene conversion tract length by modifying the transgenic system for estimating the gene conversion rate. Figure 1 illustrates such a strategy, which has a selected marker at position 432 bp. In addition to the selected ma ...
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... Based on the team’s animal model and tissue culture studies, there is a clear double whammy when alcohol and cigarette smoke are combined, Dr. Wyatt said. In the lung, chronic use of alcohol prevents the stimulation of increased cilia beating and enhanced clearance by blocking the activation of a sp ...
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... importance is underscored by its conservation from bacteriophages to humans (1). Based on amino acid sequence similarities, Rad50 belongs to the structural maintenance of chromosomes (SMC) family of proteins. Their shared amino acid sequence predicts a very distinct structure, including a bipartite ...
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... Know that the process of the information encoding by DNA being copied into mRNA is called transcription; know the steps of transcription and what happens at each step; know the enzyme complex involved in transcription is called RNA polymerase ...
Large scale features in DNA genomic signals
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... that bear a close resemblance to the DNA of independent entities like viruses and bacteria. Nevertheless, signi7cant parts of the inter-gene chromosomal DNA most likely play an important role in the control of protein synthesis, conjointly with the recently identi7ed gene regulatory networks. The ma ...
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... INVESTIGATION 1: EXAMINING RNA-SEQ DATA We will continue to focus on isoform A of transformer (referred to as tra-RA). Here we will focus on data from experiments that assess the RNA population in cells. This data can be used to help us identify exons and introns for the gene under study. All RNAs i ...
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Review Molecular Biology in Arteriosclerosis Research

... cloned into a bacteriophage lambda vector, and protein fragments encoded by the cDNA were expressed under control of a regulated lambda gene. The expressed apo E determinants were detected with an antibody to apo E. Similar schemes for the ...
arsenic life
arsenic life

... Here are some of the highlights of Rosie’s critique of the paper’s methodologies and conclusions: A. Felisa’s team claims that the cells grown in a medium with no added phosphate or arsenate failed to grow. A closer inspection of the data shows this to be inaccurate; the cells did grow, albeit more ...
DNA walker circuits - PRISM Model Checker
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Gentile, Margaret: Computational Methods for the Design of PCR Primers for the Amplification of functional Markers from Environmental Samples
Gentile, Margaret: Computational Methods for the Design of PCR Primers for the Amplification of functional Markers from Environmental Samples

... Challenges of primer design for unknown, diverse sequences The design of a primer to amplify a gene of interest from all species present differs from the applications described above, because the sequence to be amplified is not actually known and can be quite different from known sequences of the ge ...
Molecular Biology
Molecular Biology

... which is located 25 nucleotides away from the site where transcription is initiated. (B) The TATA box is recognized and bound by transcription factor TFIID, which then enables the adjacent binding of TFIIB (C). (D) The rest of the general transcription factors as well as the RNA polymerase itself as ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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