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Milestone10
Milestone10

... under the same conditions at the same times. The evidence comes from microarray experiments, i.e., yeast microarray data was clustered and these 21 genes clustered in the same group. Again, we extracted the upstream sequences for each of these 21 genes. Download the following file: http://cs.wellesl ...
werribee secondary college vce unit planner ~ 2004
werribee secondary college vce unit planner ~ 2004

... Thursday: generalized view of how information is received by a cells receptor and transducted to an effector for lipid and protein based signaling molecules Friday: ANZAC day Homework: hormone revision and exam questions Monday: PD: apoptosis as an example of the cellular response to specific signal ...
1 Synthesis, Characterization, and Biological Reactivities of Metal
1 Synthesis, Characterization, and Biological Reactivities of Metal

... ability to intercalate and cleave plasmid is interesting because, like a biological enzyme, the ligand “targets” the reactive metal center to DNA. In addition to work on the complexation of and cleavage of plasmid DNA, the interaction between saphen complexes and G-quadruplexes has also been investi ...
Novel Research Starts with GAPDH - Bio-Rad
Novel Research Starts with GAPDH - Bio-Rad

... • DNA can be extracted from the fresh plant tissue and multiple copies of the GAPDH gene can be amplified using PCR ...
Lec 16 - RNA and IT`s Structure
Lec 16 - RNA and IT`s Structure

... 2. Noncoding region 1 (NC1). The cap is followed by a region of 10 to 100 nucleotides. This region is rich in A and U residues, and does not translate protein. 3. The initiation codon is AUG in both prokaryotes and eukaryotes 4. The coding region consists of about 1,500 nucleotides on the average an ...
Determining whether Huntaway dogs treated with AAV2/8 viral
Determining whether Huntaway dogs treated with AAV2/8 viral

... on whether replication-defective Adeno-Associated Viral (AAV) vectors are new organisms for the purposes of the Hazardous Substances and New Organisms (HSNO) Act 1996 (the Act). They also requested that the EPA determine whether Huntaway dogs treated with AAV2/8 vectors are considered genetically mo ...
in Power-Point Format
in Power-Point Format

... • Small molecules can act directly on 5’-UTRs of mRNAs to control gene expression • Riboswitches –regions of 5’-UTRs that can alter structure to control gene expression after bind ligand • Region that binds ligand is called aptamer • Expression platform is other module in riboswitch: ...
Chapter 7 Operons: Fine Control of Bacterial Transcription Bacterial
Chapter 7 Operons: Fine Control of Bacterial Transcription Bacterial

... • Small molecules can act directly on 5’-UTRs of mRNAs to control gene expression • Riboswitches –regions of 5’-UTRs that can alter structure to control gene expression after bind ligand • Region that binds ligand is called aptamer • Expression platform is other module in riboswitch: ...
Diapositive 1 - LBGI Bioinformatique et Génomique Intégratives
Diapositive 1 - LBGI Bioinformatique et Génomique Intégratives

... Actin Related Proteins (ARPs) are key players in major biological processes important for cell life. In cytoskeleton activities, the ARP2/3 complex is essential for actin dynamics, ARP1 and ARP11 are involved in microtubule based vesicle trafficking, in nuclear functions (transcriptional activation, ...
Gene transfer in bacteria - McGraw Hill Higher Education
Gene transfer in bacteria - McGraw Hill Higher Education

... Genomic analyses in bacteria have created an information explosion Complete genome sequence known for hundreds of prokaryotic species and partial genome sequence known for thousands of species New avenues of research are possible with genome studies • Metagenomics – analysis of genomic DNA from a c ...
DNA-Sorb-B
DNA-Sorb-B

Characterization of the Human Gene for a Newly Discovered
Characterization of the Human Gene for a Newly Discovered

... near the addition signal (nucleotide 7152). The presence of a 17-nucleotide GT-rich segment (7185-7201) at a position (28 nucleotides downstream of the poly(A)+ signal) where similar sequences have been found to be necessary for the formation of proper 3’ termini in various mRNAs (McLauchlan et al., ...
Geuvadis Analysis Meeting
Geuvadis Analysis Meeting

... could be explainable by allele-specific expression ~4000 cases where DNA is homozygous and RNA not (!!!) remove FPs from computational or experimental artifacts (PCR artifacts?) ...
Intelligent Icons: Integrating Lite-Weight Data Mining
Intelligent Icons: Integrating Lite-Weight Data Mining

... The three files in the example are ASCII text files, each of which contains approximately 16,000 base pairs of mitochondrial DNA. Here we used string edit distance as suggested in [15] to measure the distance between file names, and Euclidean distance to measure the distance between the file icons ( ...
DNA MUTATION, REPAIR, AND TRANSPOSITION
DNA MUTATION, REPAIR, AND TRANSPOSITION

... repair DNA when excessive damage has occurred. The RecA protein interacts with single-stranded DNA, which stimulates a protease activity that is normally silent in the RecA protein. The RecA* protease activity cleaves the LexA protein. LexA is a transcriptional repressor of about 18 genes, many of w ...
Chapter 5
Chapter 5

... During the 1850s, Mendel studied genetics by doing controlled breeding experiments with pea plants. Pea plants were ideal for genetic studies because • they reproduce quickly. This enabled Mendel to grow many plants and collect a lot of data. • they have easily observed traits, such as flower color ...
Physical map of the aromatic amine and m-toluate
Physical map of the aromatic amine and m-toluate

... present in Pseudomonasputida UCC22, a derivativeof P.putida mt-2. The plasmid is 79 1kbp in size and can be divided into a restriction-site-deficient region of 51 f 1 kbp and a restriction-site-profuse region of 28 kbp which begins and ends with directly repeating sequences of at least 2 kbp in leng ...
A GENETIC LINKAGE MAP OF Phycomyces blakesleeanus
A GENETIC LINKAGE MAP OF Phycomyces blakesleeanus

... mycelium also shows responses by photo-induction of β-carotene synthesis and the initiation of sporangiophores. The responses to light have been most thoroughly analyzed, in part driven by the efforts of Nobel laureate Max Delbrück who aimed to develop Phycomyces into the “phage of vision” (Cohen e ...
Lecture 6 - U of L Class Index
Lecture 6 - U of L Class Index

... in regulating the expression of the other genes. 9 Regulator gene codes for a protein that controls transcription by binding to particular sites on DNA. This may regulate the gene in a positive manner – turns the gene on or in negative manner – turns the gene off. 9 The sequences that mark the begin ...
Genome Rearrangements Caused by Depletion of Essential DNA
Genome Rearrangements Caused by Depletion of Essential DNA

... et al. 2004; Yu et al. 2006), destabilization of target gene mRNAs through the insertion of a selectable marker in the 39-UTR of essential genes (Schuldiner et al. 2005), systematic addition of a heat-inducible degron to the amino terminus of the protein product (Labib et al. 2000), systematic gene ...
Virginia State Science Olympiad Regional Tournament
Virginia State Science Olympiad Regional Tournament

... Two (2) non-programmable non-graphing calculators, one (1) standard-size (8.5”x11”) double-sided sheet (not two one-side sheets) of paper containing any information, and writing implements may be used. Any other electronic devices are not allowed for this event; please consult the proctor about a sa ...
Introduction to Molecular Diagnostics
Introduction to Molecular Diagnostics

19.1 Somatostatin Was the First Human Peptide Hormone Produced
19.1 Somatostatin Was the First Human Peptide Hormone Produced

... express somatostatin in bacteria, the coding sequence for somatostatin must be inserted next to a bacterial promoter that is contained within a plasmid. Rather than obtaining the gene from the human genome that encodes this 14-amino acid hormone, the researchers took a different approach. As shown b ...
Supplementary Notes S1 (doc 64K)
Supplementary Notes S1 (doc 64K)

... 3. Add flanking sequence to uniform length of 1200 bp in size. Flanking regions were added to all regions less than 600 bp so that their final size will be 1200 bpto accommodate >8 50-60mer probes per region. Regions that are already 1200 bp or greater were left untouched. The resulting region is al ...
Lab Meeting, Oct 16 2003
Lab Meeting, Oct 16 2003

... • The bands which are approximately similar in size to the length of the original degenerate sequence are then cloned and sequenced to see if they share a homology to the QTL markers in tomato • The amplified PCR samples are inserted into a cloning Vector which is then inserted into E. coli. – Only ...
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Cre-Lox recombination



In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.
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