Proteins As Drugs - The Major Products of the Biotechnology Industry
... • Many normal cells express a small amount of HER2 protein on their plasma membranes in a tissue-specific pattern. • The HER2 receptor has no known ligand; however, HER2 forms heterodimers with HER1 (the epidermal growth factor receptor, EGFR), HER3 and HER4 in a complex with the ligands for these r ...
... • Many normal cells express a small amount of HER2 protein on their plasma membranes in a tissue-specific pattern. • The HER2 receptor has no known ligand; however, HER2 forms heterodimers with HER1 (the epidermal growth factor receptor, EGFR), HER3 and HER4 in a complex with the ligands for these r ...
Assaying … promoter activity
... More cryptic statements Transformants were successfully generated using (the transposome). In the first several transformation attempts, the positive control yielded between 10 and 20 colonies, while the … negative controls yielded none. … Transformants were generated using (the transposome). To te ...
... More cryptic statements Transformants were successfully generated using (the transposome). In the first several transformation attempts, the positive control yielded between 10 and 20 colonies, while the … negative controls yielded none. … Transformants were generated using (the transposome). To te ...
Biogenesis of trans-acting siRNAs, endogenous
... complexes. Thus, tasiRNA production is a model system of secondary siRNA production in plants. This review focuses on the production of tasiRNAs that are endogenous secondary siRNAs. Key words: ARGONAUTE, DICER-LIKE, microRNA, RNA-induced silencing complex, trans-acting siRNA INTRODUCTION Phenomena ...
... complexes. Thus, tasiRNA production is a model system of secondary siRNA production in plants. This review focuses on the production of tasiRNAs that are endogenous secondary siRNAs. Key words: ARGONAUTE, DICER-LIKE, microRNA, RNA-induced silencing complex, trans-acting siRNA INTRODUCTION Phenomena ...
Peer-reviewed Article PDF
... known Cas genes and instead contained unique Cas genes, including one encoding a large protein they predicted to have nuclease activity, which is now known as Cas9. Furthermore, they noted that the spacers, which are homolytic to viral genes, all share a common sequence at one end. This sequence, th ...
... known Cas genes and instead contained unique Cas genes, including one encoding a large protein they predicted to have nuclease activity, which is now known as Cas9. Furthermore, they noted that the spacers, which are homolytic to viral genes, all share a common sequence at one end. This sequence, th ...
Autism Spectrum Disorder (ASD)
... Why is the locus important? ‘The discovery of the first autism locus at genome-wide significance means that basically we found a region of the genome that is part of the chromosomes that we inherit that is highly likely to have within it a gene that predisposes to autism. This is the first step in ...
... Why is the locus important? ‘The discovery of the first autism locus at genome-wide significance means that basically we found a region of the genome that is part of the chromosomes that we inherit that is highly likely to have within it a gene that predisposes to autism. This is the first step in ...
on the enzyme
... place where the target molecule can attach. This place is called the active site. The target molecule/chemical is the substrate. ...
... place where the target molecule can attach. This place is called the active site. The target molecule/chemical is the substrate. ...
Chromosome-encoded gene cluster for the
... responsible for the complete metabolism of aniline to TCA-cycle intermediates. In the gene cluster, the first five genes (tadQTA1A2B) and the subsequent gene (tadR) were predicted to encode a multi-component aniline dioxygenase and a LysR-type regulator, respectively, while the others (tadD1C1D2C2EF ...
... responsible for the complete metabolism of aniline to TCA-cycle intermediates. In the gene cluster, the first five genes (tadQTA1A2B) and the subsequent gene (tadR) were predicted to encode a multi-component aniline dioxygenase and a LysR-type regulator, respectively, while the others (tadD1C1D2C2EF ...
Lab 7: Conjugation/Transformation
... Bacteria reproduce by asexual means (fission), but are able to transfer genes by several mechanisms: conjugation, transformation, and transduction. Conjugation is the transfer of genes via a conjugation bridge, mediated by a fertility plasmid, the F factor in E. coli. Transformation is the uptake of ...
... Bacteria reproduce by asexual means (fission), but are able to transfer genes by several mechanisms: conjugation, transformation, and transduction. Conjugation is the transfer of genes via a conjugation bridge, mediated by a fertility plasmid, the F factor in E. coli. Transformation is the uptake of ...
High-resolution haplotype structure in the human genome
... block in which it fell. Our analysis of this region of chromosome 5q31 in a European-derived population indicates the following: the region may be largely divided into discrete blocks of 10–100 kb; each block has only a few common haplotypes; and the haplotype correlation between blocks gives rise t ...
... block in which it fell. Our analysis of this region of chromosome 5q31 in a European-derived population indicates the following: the region may be largely divided into discrete blocks of 10–100 kb; each block has only a few common haplotypes; and the haplotype correlation between blocks gives rise t ...
Transposable elements, genes and recombination in a 215
... a genome designated Am that is closely related to the genome of T. urartu, the A genome donor for tetraploid and hexaploid wheats. The large genome of T. monococcum (1C =5600 Mb; Bennett and Leitch 1995) is approximately 12 times larger than the genome of rice and 40 times larger than the genome of ...
... a genome designated Am that is closely related to the genome of T. urartu, the A genome donor for tetraploid and hexaploid wheats. The large genome of T. monococcum (1C =5600 Mb; Bennett and Leitch 1995) is approximately 12 times larger than the genome of rice and 40 times larger than the genome of ...
Course leader - University of Warwick
... In order to search the database it is necessary to define some fields. It is useful to supply as much information as is known about the protein. For example searching under Lysozyme alone reveals 121 hits. Useful fields to complete include: Description; Organism from which the protein is from; Keywo ...
... In order to search the database it is necessary to define some fields. It is useful to supply as much information as is known about the protein. For example searching under Lysozyme alone reveals 121 hits. Useful fields to complete include: Description; Organism from which the protein is from; Keywo ...
Methods for detection of point mutations
... Limit of detection. Approximately one mutant cell is detectable in the presence of 10 normal cells [22]. Methods of detection. Initially, fragments were labeled radioactively and detected by autoradiography. Nonradioactive detection, e.g., by silver staining, is feasible. Performance and quality ass ...
... Limit of detection. Approximately one mutant cell is detectable in the presence of 10 normal cells [22]. Methods of detection. Initially, fragments were labeled radioactively and detected by autoradiography. Nonradioactive detection, e.g., by silver staining, is feasible. Performance and quality ass ...
Assessment by Molecular Dynamics Simulations of the Structural
... Sp1 consists of three consecutive zinc fingers that recognize a number of nucleotide sequences different from, but related to and sometimes overlapping, those recognized by the structurally better characterized early growth response protein 1 (EGR1, also known as Zif268, Krox-24, and NGFI-A). The ac ...
... Sp1 consists of three consecutive zinc fingers that recognize a number of nucleotide sequences different from, but related to and sometimes overlapping, those recognized by the structurally better characterized early growth response protein 1 (EGR1, also known as Zif268, Krox-24, and NGFI-A). The ac ...
RNA-Mediated Programming of Developmental
... line genome of the micronuclei remains wild type. The maternal deletion effect was observed with all sequences tested, including noncoding sequences; targeting a subtelomeric gene resulted in terminal deletions, while targeting an internal sequence resulted in heterogeneous internal deletions of the ...
... line genome of the micronuclei remains wild type. The maternal deletion effect was observed with all sequences tested, including noncoding sequences; targeting a subtelomeric gene resulted in terminal deletions, while targeting an internal sequence resulted in heterogeneous internal deletions of the ...
Chromosomal G + C Content Evolution in Yeasts
... (Gerton et al. 2000; Birdsell 2002; Mancera et al. 2008). For several years, it was unclear which of these two factors drove the other: whether a high local G þ C content could increase the local recombination rate (Gerton et al. 2000; Petes and Merker 2002) or whether the presence of a recombinatio ...
... (Gerton et al. 2000; Birdsell 2002; Mancera et al. 2008). For several years, it was unclear which of these two factors drove the other: whether a high local G þ C content could increase the local recombination rate (Gerton et al. 2000; Petes and Merker 2002) or whether the presence of a recombinatio ...
Identification of Human Polymorphisms in the Phenylthio
... Two ‘dry lab’ versions of this project have been developed for instructors who do not have the time and/or laboratory resources to do DNA isolation, PCR, and DNA sequencing. In the ‘dry lab’ activities, the project is once again introduced using the Power Point slides, and all study participants t ...
... Two ‘dry lab’ versions of this project have been developed for instructors who do not have the time and/or laboratory resources to do DNA isolation, PCR, and DNA sequencing. In the ‘dry lab’ activities, the project is once again introduced using the Power Point slides, and all study participants t ...
Functional Divergence of the Nuclear Receptor NR2C1
... receptors (NRs) are attractive candidates for a combined evolutionary and functional investigation of hominids (e.g., the clade that includes modern great apes and their last common ancestors). As transcription factors, NRs control many aspects of development, metabolism, reproduction, and endocrine ...
... receptors (NRs) are attractive candidates for a combined evolutionary and functional investigation of hominids (e.g., the clade that includes modern great apes and their last common ancestors). As transcription factors, NRs control many aspects of development, metabolism, reproduction, and endocrine ...
Identifying Unknown Bacteria Using Biochemical
... presence of that particular enzyme(s). Each positive result is used in generating a five-digit number. This five-digit number, or “biocode,” can then be looked up in either the Enterotube or Oxi/Ferm tube code book, as appropriate; the number will correspond to a species of bacteria that produces th ...
... presence of that particular enzyme(s). Each positive result is used in generating a five-digit number. This five-digit number, or “biocode,” can then be looked up in either the Enterotube or Oxi/Ferm tube code book, as appropriate; the number will correspond to a species of bacteria that produces th ...
Analysis of the 3′-terminal nucleotide sequence of vesicular
... general approach require the use of a "phased" primer for reverse transcription, that is, an oligonucleotide of general formula (dT)n-dN or (dT) -dN,-dN_, which will anneal specifically to the mRNA at the junction of the poly(A) tract and heteropolymeric RNA (14). We have used this general approach ...
... general approach require the use of a "phased" primer for reverse transcription, that is, an oligonucleotide of general formula (dT)n-dN or (dT) -dN,-dN_, which will anneal specifically to the mRNA at the junction of the poly(A) tract and heteropolymeric RNA (14). We have used this general approach ...
STPM 2011 - Soalan-Percubaan-STPM
... both alleles in a heterozygote are dominant and fully expressed in phenotype B both alleles that give partial influence in heterozygous form C more than two alternative forms a particular gene that occupy the same locus D genes that results in the death of an organism. ...
... both alleles in a heterozygote are dominant and fully expressed in phenotype B both alleles that give partial influence in heterozygous form C more than two alternative forms a particular gene that occupy the same locus D genes that results in the death of an organism. ...
Exercise II - GEP Community Server
... 13. Compare the Augustus prediction and the BLASTN evidence. You will find that they share the same exon-intron structure, but differ in the overall lengths: the gene model starts and ends further down-stream than the BLASTN evidence. 14. Use Exon Detail Editor to adjust the lengths of the flanking ...
... 13. Compare the Augustus prediction and the BLASTN evidence. You will find that they share the same exon-intron structure, but differ in the overall lengths: the gene model starts and ends further down-stream than the BLASTN evidence. 14. Use Exon Detail Editor to adjust the lengths of the flanking ...
GIN Transposons: Genetic Elements Linking Retrotransposons and
... built including them and also sequences from Mdg1 retrotransposons, which were to be used as outgroups. The results are shown in figure 1, and the aligned sequences can be found in supplementary file 2 (Supplementary Material online). The detailed analysis of these new sequences totally changes our ...
... built including them and also sequences from Mdg1 retrotransposons, which were to be used as outgroups. The results are shown in figure 1, and the aligned sequences can be found in supplementary file 2 (Supplementary Material online). The detailed analysis of these new sequences totally changes our ...
BMC Genomics
... tes present a bidirectional replication starting from the origin of replication and reading in both directions until reaching a terminus (replication inset). The bidirectional replication therefore defines a leading and a lagging strand in the double helix. In the contrary, nucleotide sequence readi ...
... tes present a bidirectional replication starting from the origin of replication and reading in both directions until reaching a terminus (replication inset). The bidirectional replication therefore defines a leading and a lagging strand in the double helix. In the contrary, nucleotide sequence readi ...
Gene regulation in three dimensions
... Last, but not least I would like to thank Julie my fiancé who has supported me and been there for me when I needed it the most. K.T.W ...
... Last, but not least I would like to thank Julie my fiancé who has supported me and been there for me when I needed it the most. K.T.W ...
Cre-Lox recombination
In the field of genetics, Cre-Lox recombination is known as a site-specific recombinase technology, and is widely used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems.The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1.Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally.The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system.